Abstract:
:Cytokines are the main agents known to regulate Th1 / Th2 commitment, where they may operate through paracrine activity within clusters of T cells gathered around dendritic cells (DC). An in vitro system is used here to test this possibility, using clusters around DC composed of naive TCR-transgenic ovalbumin peptide 323 - 339-specific CD4(+) T cells as targets plus TCR-transgenic pigeon cytochrome C peptide 88 - 104-specific CD4(+) polarized Th1 or Th2 cells as inducers. The polarized inducer cells exerted their maximum effect when the two T cell populations were activated within the same cluster, implemented by allowing a single DC to present both their epitopes. This finding thus supports the paracrine hypothesis. The system was then employed to explore the role of individual cytokines by means of inhibition by monoclonal antibodies. Development of Th2 commitment proved strictly dependent on the IL-4 produced by the Th2 inducers. For Th1 commitment, IFN-gamma and IL-12 were both needed, but with IFN-gamma required only during the initial period of culture. The rapid timing observed under these conditions places constraints on the molecular basis of commitment, and appears accurately to reflect the physiological response in vivo.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Schuhbauer DM,Mitchison NA,Mueller Bdoi
10.1002/(SICI)1521-4141(200005)30:5<1255::AID-IMMUkeywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
1255-62issue
5eissn
0014-2980issn
1521-4141pii
10.1002/(SICI)1521-4141(200005)30:5<1255::AID-IMMUjournal_volume
30pub_type
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