Abstract:
:In humans and in BALB/c mice, immune responses to the hormone insulin use evolutionarily related VHV (human) and VHIX (murine) gene families. To determine if these structural relationships include regulatory elements, BALB/c mice were pretreated with autologous immunoglobulin G (IgG) monoclonal antibodies (mAb) that recognize shared idiotopes on human anti-insulin antibodies and the subsequent immune response to human insulin assessed. One mAb, Id227, was found to augment and accelerate the insulin response by inducing a human idiotype that is expressed on both insulin-binding and non-insulin-binding BALB/c antibodies. Analysis of VH gene utilization by Id227 shows that it expresses a VHIX gene similar to that of anti-insulin mAb 125, but the anti-Id has no anti-insulin activity. Using DNA amplification, four germ-line VHIX genes were isolated from BALB/c liver DNA and sequence analysis shows that the anti-insulin and anti-Id are derived from the same germ-line gene. Consistent with its role as a regulatory idiotype, IgG Id227 entirely preserves germ-line sequence in the complementary determining regions and contains only three mutations in framework regions. These studies show that both structural and regulatory features of immune responses to conserved self antigens extend beyond species boundaries.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Thomas JWdoi
10.1002/eji.1830220938keywords:
subject
Has Abstractpub_date
1992-09-01 00:00:00pages
2445-8issue
9eissn
0014-2980issn
1521-4141journal_volume
22pub_type
杂志文章abstract::The HIV-1 Tat protein plays a critical role in the pathogenesis of HIV and has been considered as a candidate vaccine antigen. In an effort to design a non-invasive vaccination strategy against HIV-1 that stimulates the induction of systemic and mucosal immune responses, we studied the transcutaneous delivery of a syn...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425313
更新日期:2004-12-01 00:00:00
abstract::Using a new mAb, 2F1, we characterize a mouse natural killer (NK) cell antigen termed 'killer cell lectin-like receptor G1' (KLRG1; formerly mouse MAFA or 2F1-Ag). KLRG1 is expressed on 30-60% of murine NK cells, and a small fraction of T cells, and is composed of a homodimer of glycosylated 30-38-kDa subunits. Striki...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200003)30:3<920::AID-IMMU920>3.0
更新日期:2000-03-01 00:00:00
abstract::The glycolipid alpha-galactosylceramide (alpha-GalCer) is a potent activator of invariant natural killer T (iNKT) cells and has been shown to be an effective agent against cancer, infections and autoimmune diseases. The effectiveness of alpha-GalCer and its alkyl chain analogues depends on efficient loading and presen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737518
更新日期:2008-03-01 00:00:00
abstract::Leukosialin (CD43) is a major glycoprotein of T lymphocytes which has an extracellular domain of 45 nm in length that is heavily O-glycosylated. Monoclonal antibodies (mAb) to the extracellular domain of human leukosialin induce aggregation of T lymphocytes, monocytes and some cell lines that express leukosialin. The ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221015
更新日期:1992-10-01 00:00:00
abstract::The involvement of the CD2 (T11) molecule, an alternative activation pathway for T lymphocytes, in the regulation of tumor necrosis factor (TNF)-alpha/TNF receptor system in human T lymphocytes has been investigated. It has been found that both TNF-alpha synthesis and secretion were induced after incubation of purifie...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221219
更新日期:1992-12-01 00:00:00
abstract::Previously we have reported on the production of two sets of human monoclonal antibodies reacting with mouse mammary tumor virus (MMTV) and human mammary tumor virus (HuMTV) cross-reacting antigens. B11 is a monoclonal IgG generated by the human-human hybridoma technique using axillary lymph node of breast cancer pati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181109
更新日期:1988-11-01 00:00:00
abstract::B cell development is dependent on both direct interactions with stromal cells and their secreted cytokines. The precise mechanisms by which these interactions regulate B cell differentiation are currently unknown. We report here that a novel growth factor thymic stromal-derived lymphopoietin (TSLP) can replace the ac...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260103
更新日期:1996-01-01 00:00:00
abstract::Since signals via CD40 and the B cell receptor are known to synergize to induce B cell activation, we have analyzed the pocket protein/E2F complexes in mouse B lymphocytes following stimulation by anti-IgM, anti-CD40, alone or together. We find that E2F4 and DP1 form the predominant E2F heterodimers in the G0 and G1 p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199910)29:10<3380::AID-IMM
更新日期:1999-10-01 00:00:00
abstract::In this study, we investigated whether the phenotype-related differentiation of human 2H4+ (CD45R) naive cells to 2H4- (CDw29) memory CD4 cells corresponded to modulation of interleukin (IL) 2, IL 4, interferon (IFN)-gamma and granulocyte-monocyte colony-stimulating factor (GM-CSF) gene expression, a phenomenon which ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190908
更新日期:1989-09-01 00:00:00
abstract::This evidence-based clinical guideline provides consensus-recommendations for the treatment and care of patients with primary antibody deficiencies (PADs). The guideline group comprised 20 clinical and scientific expert associations of the German, Swiss, and Austrian healthcare system and representatives of patients. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048713
更新日期:2020-10-01 00:00:00
abstract::The expression of an X-linked defect in the CBA/N strain of mice has been found to result in a number of immune abnormalities. These include low responsiveness to antigens and a greatly reduced ability to respond to many of the common B cell mitogens. An in vitro manifestation of this condition is the virtual inabilit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220419
更新日期:1992-04-01 00:00:00
abstract::We have previously identified a molecule on the T cell surface, which, in addition to CD2 is involved in the rosette phenomenon. This is a 32-kDa single polypeptide chain which we have termed E2. The studies reported here show striking patterns on the glycosylation status of E2. It is a heavily sialylated and glycosyl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190813
更新日期:1989-08-01 00:00:00
abstract::Several groups have described that a low percentage of in vitro cultured T cell receptor (TcR) gamma/delta cells express CD8. Contrary to TcR alpha/beta cells, however, CD8 on these TcR gamma/delta cells was shown to be a CD8 alpha homodimer. We describe here that addition of interleukin-7 (IL-7) to a short-term in vi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220902
更新日期:1992-09-01 00:00:00
abstract::Natural killer (NK) cell activity can be stimulated by different surface receptors. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating human NK cell cytotoxicity and cytokine production. Here we show that stimulation of human NK cells by anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636146
更新日期:2006-12-01 00:00:00
abstract::IL-10, produced by a wide variety of cells, is a highly pleiotropic cytokine that plays a critical role in the control of immune responses. However, its regulatory activity in tumor immunity remains poorly understood. In this study, we report that IL-10 deficiency robustly suppressed the formation of malignant pleural...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048574
更新日期:2020-11-01 00:00:00
abstract::In this work the suppressive activity of splenic T cells from young and aged BALB/c mice infected with Trypanosoma cruzi were compared and correlated with the development of autoimmune myocarditis. The T cells from young adult BALB/c mice with acute T. cruzi infection exhibit suppressor activity when added to full all...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231033
更新日期:1993-10-01 00:00:00
abstract::The use of fetal bovine serum (FBS) for the culture and expansion of mesenchymal stromal cells (MSCs) limits their possible clinical applications. Although some recent studies recommended substituting FBS with human platelet lysate (HPL) for the expansion of MSCs for clinical use, the functional capacity of the expand...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141542
更新日期:2011-11-01 00:00:00
abstract::Cholera toxin (CT) can function as a potent adjuvant in the mucosal immune response. However, we have found that treatment of A20-HL murine B lymphoma cells with CT severely inhibits the presentation of ovalbumin (OVA) to cells of the T cell clone 42-6A specific for OVA(323-339)/I-Ad, whereas it does not affect the pr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199905)29:05<1561::AID-IMM
更新日期:1999-05-01 00:00:00
abstract::By combining velocity and linear density fractionations as well as target cell rosetting techniques we have isolated and morphologically identified the human effector cell type responsible for spontaneous, trypsin-augmentable cytotoxicity against chicken red cells and human myeloma cell line targets. This cell is a la...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830071104
更新日期:1977-11-01 00:00:00
abstract::Antigenic peptides have been found associated with heat shock proteins (HSP) including cytoplasmic HSP70 and heat shock cognate protein 70 as well as the endoplasmic reticulum-resident glucose-regulated protein 94. Recently, HSP70 transfection has been reported to increase MHC class I cell surface expression and antig...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<3925::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::Regulatory T (Treg) cells are critically important for the maintenance of immunological tolerance. Both centrally arising natural nTreg cells and those emerging in the periphery in response to TGF-β, iTreg cells, play a role in the control of unwanted immune responses. Treg cells adopt multiple mechanisms to inhibit e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142207
更新日期:2012-06-01 00:00:00
abstract::Atopic dermatitis (AD) or eczema is the most common chronic inflammatory skin disease. It is a multifactorial disease with local and systemic immune changes. Current therapies focus on restoring the local skin barrier or inhibiting immune responses. In this issue of the European Journal of Immunology, Sehra et al. [Eu...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201646708
更新日期:2016-11-01 00:00:00
abstract::In this study, after immunization with NK cells from a KIR2DS5(+) donor and screening on cell transfectants expressing different members of the killer immunoglobulin-like receptor (KIR) family, we generated a mAb, DF200, reacting with several KIR2D receptors including KIR2DL1/L2/L3, KIR2DS1/S2 and KIR2DS5. By the anal...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838434
更新日期:2008-08-01 00:00:00
abstract::We investigated the effect of recombinant murine interleukin 4 (IL 4) in the absence or presence of recombinant murine interferon-gamma (IFN-gamma) on adherent bone-marrow macrophages (M phi), peritoneal exudate and resident peritoneal M phi from susceptible BALB/c M phi, which were pulse-infected with Leishmania majo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210213
更新日期:1991-02-01 00:00:00
abstract::Interleukin-HP1 (HP1) is a murine T cell-derived lymphokine, originally described as a growth factor for B cell hybridomas and plasmacytomas, that was recently shown to stimulate growth and differentiation of normal B and T lymphocytes. Here, we describe a cDNA for HP1 that was isolated from a library prepared using m...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180202
更新日期:1988-02-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection frequently develops into liver disease and is accompanied by extra-hepatic autoimmune manifestations. The tetraspanin CD81 is a putative HCV receptor as it binds the E2 envelope glycoprotein of HCV and bona fide HCV particles. Here we show that HCV E2 binding to CD81 on human ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200101)31:1<166::aid-immu166>3.0
更新日期:2001-01-01 00:00:00
abstract::Spleen cells from BALB/c mice were fused with the nonsecreting myeloma line X63.Ag8.6.5.3 seven days after immunization with NP-CG (4-hydroxy-3-nitrophenyl) acetyl-chicken gamma-globulin. The hybrid cell lines obtained were analyzed for heavy and light chain distribution, fine specificity, and idiotype. The majority o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830111109
更新日期:1981-11-01 00:00:00
abstract::Viruses encode immune evasion mechanisms to survive in the immunocompetent host. Chemokines mediate the migration of immune cells and their critical role in immunity is emphasized by the numerous virus-encoded strategies to modulate their activity. Evidence published in this issue of the European Journal of Immunology...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737215
更新日期:2007-04-01 00:00:00
abstract::IgG complexes bind to Fc receptor family members FcgammaRI (CD64), FcgammaRII (CD32) and FcgammaRIII (CD16), activating cell MAPK and PI3K resulting in increased cytokine production from particular leukocytes. The signaling molecules involved in cytokine production after cross-linking CD16 have not been determined in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838801
更新日期:2009-02-01 00:00:00
abstract::The active role of chemokines in the central nervous system (CNS) during the pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been clearly established. In this study the expression pattern of several chemokines and cytokines was elucidated using reverse transcription-PCR and immunohistochemistry in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1911::AID-IMMU1911>3
更新日期:2000-07-01 00:00:00