Abstract:
:Ptak and Askenase showed that both alphabeta and gammadelta cells are required for transfer of contact sensitivity (CS). This study confirms that day 4 immune cells depleted of gammadelta cells fail to transfer CS to trinitrochlorobenzene (TNP-Cl) systemically and demonstrates that administration of anti-gammadelta monoclonal antibodies (mAb) in vivo abolishes the CS reaction. Moreover, gammadelta cells accumulate at the antigen challenge site: these cells have the unusual phenotype CD8alpha+, CD8beta-, IL-4 R+ which we suggest is due to their state of activation. Following immunization with contact sensitizer on the skin, the absolute number of gammadelta cells increases in the regional lymph nodes with a peak at 4 days. Of the gammadelta cells, 80 %, both in the lymph nodes of TNP-Cl-immune mice and accumulating at the antigen challenge site are Vgamma3+. The gammadelta cells expressing Vgamma3, which is characteristic of dendritic epithelial T cells (DETC), obtained 4 days after sensitization, proliferate in response to interleukin (IL)-7, but only poorly to IL-2 and IL-4. They also respond to concanavalin A and immobilized anti-gammadelta mAb, but not to haptens or heat-shocked syngeneic spleen cells. Furthermore, injection of mice with mAb to IL-7 inhibits accumulation of Vgamma3+ cells both in the lymph nodes after skin sensitization and at the antigen-challenge site. Altogether, these results strongly support the view that DETC are related to, or the original source of, the gammadelta cells found in the lymph node after skin sensitization and at the site of challenge, and that IL-7 is implicated in these phenomena.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Dieli F,Asherson GL,Sireci G,Dominici R,Gervasi F,Vendetti S,Colizzi V,Salerno Adoi
10.1002/eji.1830270131subject
Has Abstractpub_date
1997-01-01 00:00:00pages
206-14issue
1eissn
0014-2980issn
1521-4141journal_volume
27pub_type
杂志文章abstract::Six different cell-mediated lympholysis (CML) combinations were established, four of which generated effector cells against the I-E, and two against the I-A molecule. The cell surface phenotype of effector cells was then determined by depletion of cytotoxic T lymphocyte (CTL) activity with antisera and rabbit compleme...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110611
更新日期:1981-06-01 00:00:00
abstract::Hybrid virus-like particles (VLP) were prepared by self-assembly of the modified porcine parvovirus (PPV) VP2 capsid protein carrying a CD8+ or CD4+ T cell epitope. Immunization of mice with a single dose of these hybrid pseudo-particles, without adjuvant, induced strong cytotoxic T lymphocyte and T helper (Th) respon...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199804)28:04<1401::AID-IMM
更新日期:1998-04-01 00:00:00
abstract::Lipids are key regulators of immune responses. In this study we investigated the direct impact of oxidized phospholipids (ox-PL) on T cell activation and function. We could demonstrate that ox-PL strongly inhibit proliferation of purified human T cells induced with anti-CD3/CD28 or anti-CD3/CD63 mAb, whereas prolifera...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737619
更新日期:2008-03-01 00:00:00
abstract::Twelve continuous rat tissue cultured mast cell (MC) lines were established by prolonged culture of rat peritoneal MC in the absence of added factors or feeder layers. Two of these lines, RCMC1 and RCMC2, have been briefly described previously, seven others are now also described. Both RCMC1 and RCMC2 lack a marker ch...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200127
更新日期:1990-01-01 00:00:00
abstract::CD59 is a 18-20-kDa membrane glycoprotein that inhibits formation of the membrane attack complex of complement (C) on homologous cells. In the present study we analyzed the expression and function of CD59 on human endothelial cells. Immunohistochemical analysis of renal cortex demonstrated a predominant expression of ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220324
更新日期:1992-03-01 00:00:00
abstract::CD72 has been reported to regulate BCR-mediated signals both positively and negatively. SHP-1 and Grb2 bind, respectively, to ITIM1 and ITIM2 of CD72. We generated transformed B cell lines with an immature phenotype following J2 virus infection of splenocytes from CD72(-/-) and wild-type (Wt) mice. The transformed lin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425775
更新日期:2005-05-01 00:00:00
abstract::Barrier surfaces of multicellular organisms are in constant contact with the environment and infractions to the integrity of epithelial surfaces is likely a frequent event. Interestingly, components of the immune system, that can be activated by environmental compounds such as the microbiota or nutrients, are interspe...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201747183
更新日期:2018-01-01 00:00:00
abstract::The recognition of lipid antigens by T cells is a complex and fascinating phenomenon. The MHC-like molecules of the CD1 family have evolved to present a wide variety of both self and foreign lipids for recognition by T-cell receptors. While much progress has been made in our understanding of the NKT cells that recogni...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201141408
更新日期:2011-03-01 00:00:00
abstract::Single-positive thymocytes are the immediate precursors of peripheral recent thymic emigrants (RTE) which develop into mature peripheral T cells. The functional ability of RTE is unclear but their state of differentiation may be relevant to the development of tolerance to peripheral "self" antigens. Since RTE are diff...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240738
更新日期:1994-07-01 00:00:00
abstract::Vgamma9/Vdelta2+ T cells specifically recognize Mycobacterium tuberculosis in vitro and are precociously recruited in early mycobacterial lesions. Even if gammadelta T cells are only fortuitously detected in granulomas or bronchoalveolar lavages of patients with active pulmonary tuberculosis, a role in shaping the mat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199806)28:06<1798::AID-IMM
更新日期:1998-06-01 00:00:00
abstract::T-cell activation and the subsequent transformation of activated T cells into T-cell blasts require profound changes in cell volume. However, the impact of cell volume regulation for T-cell immunology has not been characterized. Here we studied the role of the cell-volume regulating osmolyte transporter Taut for T-cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141690
更新日期:2012-04-01 00:00:00
abstract::Granzyme A (TSP-1) is a serine esterase expressed in cytotoxic T and natural killer (NK) cell lines. Its presence in in vivo primed T cells is disputed at present. Here we show that normal rat NK cells contain granzyme A in vivo and that its expression is augmented by their short-term in vitro treatment with IL 2. Gra...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190328
更新日期:1989-03-01 00:00:00
abstract::Continuous antigen stimulation of CD4(+)CD25(-) T cells leads to generation of adaptive CD4(+)CD25(+)FOXP3(+) regulatory T (T(R)) cells. Here, we show that highly suppressive adaptive CD8(+)CD25(+)FOXP3(+) T cells can be generated in the same manner by continuous antigen stimulation in the presence of CD14(+) monocyte...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737529
更新日期:2008-03-01 00:00:00
abstract::Previous studies have suggested that T-lymphocyte dysfunction might be attributable to nitrative stress induced by reactive nitrogen species (RNS). In this manuscript, we explored this hypothesis and provided a direct demonstration of the inhibitory effects of RNS on human T-cell signaling, activation, and migration. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040868
更新日期:2011-07-01 00:00:00
abstract::Acute inflammatory activation of macrophages by Toll-like and related receptors is characterized by transient activation of MAPK-, NF-κB- and IRF-mediated signaling pathways and expression of pro-inflammatory genes. This activation state is inherently unstable and often transitions into a state of 'tolerance' characte...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201141783
更新日期:2011-09-01 00:00:00
abstract::One of the clear paradoxes in tumor immunology is the fact that cross-presentation of cell-associated tumor antigens to CD8(+) T cells is efficient, yet CTL generation is weak, and tumors continue to grow. We examined, for the first time whether this may be due to alterations in the phenotype or function of cross-pres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940153
更新日期:2010-06-01 00:00:00
abstract::The human T cell leukemia virus type-1 (HTLV-1) is the etiologic agent of adult T cell leukemia (ATL). Since the HTLV-I-encoded transactivator Tax has been shown to activate many cellular genes including cytokine genes interleukin (IL-)1alpha, 2, 5, 6, 8, 10 and 15, we ask whether Tax also affects IL-4 expression. In ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200109)31:9<2623::aid-immu2623>3
更新日期:2001-09-01 00:00:00
abstract::Under physiological conditions, activation of CD4+ T cells by major histocompatibility complex (MHC)antigen complexes requires engagement of both the T cell receptor and the CD4 molecule. However, CD4 ligands binding to the CD4 molecule has also been shown to inhibit T cell proliferation and interleukin (IL)-2 product...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270209
更新日期:1997-02-01 00:00:00
abstract::The effect of fractionated total lymphoid irradiation (TLI) on the maturation of B cells was examined in mice. At various times after irradiation of the mice, their spleen cells were tested for the mitogenic responses to dextran sulfate and lipopolysaccharide. In parallel the cells were stained with fluorescent anti-T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130109
更新日期:1983-01-01 00:00:00
abstract::We have earlier described (Cardell, S. and Sander, B., Eur. J. Immunol. 1990. 20:389) mitogen-induced production of interleukin (IL)2, IL4 and IL5 mRNA by murine spleen cells, analyzed by in situ hybridization. In the present study we have investigated the potential of CD8 T cells to produce these interleukins, normal...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211028
更新日期:1991-10-01 00:00:00
abstract::To determine the molecular mechanisms of T cell stimulation by staphylococcal enterotoxin A (SEA), we examined the expression of T cell receptor (TcR) V beta on the T cells from four strains of mice stimulated in vitro with SEA, using flow cytometric analysis for the number of T cells bearing V beta 3, V beta 6, V bet...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200323
更新日期:1990-03-01 00:00:00
abstract::The transcription factor Hhex (hematopoietically expressed homeobox gene) is critical for development of multiple lymphoid lineages beyond the common lymphoid progenitor. In addition, Hhex regulates hematopoietic stem cell (HSC) self-renewal, emergency hematopoiesis, and acute myeloid leukemia initiation and maintenan...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948371
更新日期:2020-07-01 00:00:00
abstract::MHC class I molecules are important components of immune surveillance. There are no available methods to directly visualize and determine the quantity and distribution of MHC/peptide complexes on individual cells or to detect such complexes on antigen-presenting cells in tissues. MHC-restricted recombinant antibodies ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737524
更新日期:2008-06-01 00:00:00
abstract::Multinucleated giant cells (MGC), which are a common feature of various pathologic states, were generated in vitro by cytokine-stimulation of human peripheral blood monocytes. As expected, conditioned medium, i.e. the supernatant of concanavalin A-stimulated peripheral blood mononuclear cells, readily caused generatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200807
更新日期:1990-08-01 00:00:00
abstract::We identified the presence in the Aleutian skate, Bathyraja aleutica, of two classes of immunoglobulins (Ig), a high molecular weight Ig analogous to mammalian IgM and a low molecular weight Ig, similarly to the spiny rasp skate, Raja kenojei, (Kobayashi, K. et al., Mol. Immunol. 1984. 21: 397), using an immunological...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150916
更新日期:1985-09-01 00:00:00
abstract::The purpose of this work was to study the interrelationship of proliferation and secretion of plasminogen activator (PA) and interferon (IFN) by murine macrophages. For induction of macrophage proliferation and secretion of PA, concanavalin A (Con A) was used. Secretion of IFN was induced by polyinosinic polycytidylic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130210
更新日期:1983-02-01 00:00:00
abstract::For long-term attack on tumor cells in patients with prostate cancer, induction of cytolytic T cells is desirable. Several lineage-specific target proteins are known and algorithms have identified candidate MHC class I-binding peptides, particularly for HLA-A*0201. We have designed tolerance-breaking DNA fusion vaccin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141518
更新日期:2011-08-01 00:00:00
abstract::To understand further the roles that negative regulatory signals may play in B cell immune responses, we compared three inhibitors of B cell proliferation: cross-linking CD19 with monoclonal antibody (mAb), signaling through Fc receptors by intact anti-mu mAb, and transforming growth factor-beta (TGF-beta). Each agent...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200516
更新日期:1990-05-01 00:00:00
abstract::Type B T cells recognize peptide-MHC class II (pMHCII) isoforms that are structurally distinct from those recognized by conventional type A T cells. These alternative type B conformers result from peptide loading in the absence of HLA-DM. Type A conformers are more stable than type B pMHCII conformers but bind the sam...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344065
更新日期:2014-04-01 00:00:00
abstract::Studies of experimental autoimmune encephalomyelitis (EAE) in rodents have revealed that encephalitogenic T cell lines reactive with myelin basic protein (BP) are frequently dominated by clones expressing a restricted T cell receptor repertoire. Using the rat EAE model, we have begun to examine the basis for clonal do...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250114
更新日期:1995-01-01 00:00:00