Abstract:
:The spleen is anatomically and functionally divided into two compartments: the red pulp, where particles are effectively removed from the blood, and the white pulp, where specific immune responses are generated. Here the isolation of white pulp from red pulp is described, allowing a detailed analysis of the cellular components of both red and white pulp separately. A striking abundance of memory T cells was found in the white and red pulp with an overall ratio of T and B cells in the white pulp being similar to that in lymph nodes. Both NK and gamma delta T cells can be found in white pulp and lymph nodes, but granulocytes are absent. The distribution of dendritic cell subsets showed significant differences between white pulp and lymph nodes. Furthermore, short-term homing experiments showed that migration of lymphocytes into the white pulp greatly exceeded that into lymph nodes, with significant differences in migration of various lymphocytes subsets. This suggests a different migration and retention mechanism in the white pulp. This new isolation technique will allow further analysis of the functional capacities of the splenic compartments.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Nolte MA,Hoen EN,van Stijn A,Kraal G,Mebius REdoi
10.1002/1521-4141(200002)30:2<626::AID-IMMU626>3.0keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
626-34issue
2eissn
0014-2980issn
1521-4141pii
10.1002/(SICI)1521-4141(200002)30:02<626::AID-IMMUjournal_volume
30pub_type
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