Abstract:
:Two interleukin 5 (IL5)-specific retroviral expression vectors have been constructed containing the neomycin gene as selectable marker and either the mouse IL5 cDNA region or the rat genomic IL5 gene under the control of the thymidine kinase promoter. High viral titer supernatants derived from the transfected or infected packaging cell line psi 2 were used to infect the two cell lines B13 and T88M whose growth is dependent on exogenous IL 5. Infection resulted in G418 resistance and IL 5-independent growth with a high frequency. Clones were established which secrete between 2 and greater than 1000 U IL5. The proliferation of the IL5 autocrine growing cells could be inhibited by an antibody directed against the IL5 receptor indicating that they grow as a result of the endogenously produced IL5. Regardless of the amount of IL5 they produced, all of the clones were highly tumorigenic in nucle mice. The phenotype of the tumors was indistinguishable from that of the injected cells. T88M or B13 cells infected with a control virus neither produced IL5, nor became factor independent, nor produced tumors. Together, the IL5 gene transfer and expression into IL5-dependent growing cells are in accordance with the "autocrine growth" hypothesis and contrast analogous experiments with IL4.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Blankenstein T,Li WQ,Uberla K,Qin ZH,Tominaga A,Takatsu K,Yamaguchi N,Diamantstein Tdoi
10.1002/eji.1830201226subject
Has Abstractpub_date
1990-12-01 00:00:00pages
2699-705issue
12eissn
0014-2980issn
1521-4141journal_volume
20pub_type
杂志文章abstract::Dendritic cells (DC) are unique in their ability to present antigen to naive T cells, and therefore play a central role in the initiation of immune responses. Characterization of DC-specific genes may help to unravel the mechanism underlying their potent antigen presenting capacity. Here we describe the identification...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3585::AID-IMMU3585>
更新日期:2000-12-01 00:00:00
abstract::Patients with X-linked lymphoproliferative syndrome type 2 (XLP-2) (BIRC4 deficiency) suffer from hyperinflammation often observed during the conditioning regimen prior to allogeneic bone marrow transplant. This article shows that in mice hematopoietic recipient cells contribute to graft-versus-host disease by the sec...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201847818
更新日期:2019-03-01 00:00:00
abstract::Human natural killer (NK) cells can be subdivided into two populations based on the density of cell surface CD56 antigen. The great majority (approximately 90%) of NK cells express CD56 at low levels (the CD56dim phenotype), whereas a small NK cell subset (approximately 10%) exhibits approximately fivefold greater den...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220102
更新日期:1992-01-01 00:00:00
abstract::DNA vaccination is an efficient way to induce CD8+ T cell memory, but it is still unclear to what extent such memory responses afford protection in vivo. To study this, we induced CD8+ memory responses directed towards defined viral epitopes, using DNA vaccines encoding immunodominant MHC class I-restricted epitopes o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323928
更新日期:2003-07-01 00:00:00
abstract::CD23-deficient and anti-CD23 monoclonal antibody-treated mice were used to investigate the role of the low-affinity receptor for IgE (CD23) in allergic airway inflammation and airway hyperresponsiveness (AHR). While there were no significant differences in ovalbumin (OVA)-specific IgE titers and tissue eosinophilia, e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199909)29:09<2957::AID-IMM
更新日期:1999-09-01 00:00:00
abstract::Follicular helper T (Tfh) cells provide crucial help to germinal center B (GCB) cells for proper antibody production, and a specialized subset of regulatory T cells, follicular regulatory T (Tfr) cells, modulate this process. However, Tfr-cell function in the GC is not well understood. Here, we define Tfr cells as a C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546094
更新日期:2016-05-01 00:00:00
abstract::Helicobacter pylori is a Gram-negative bacterium that causes a variety of gastrointestinal diseases, such as duodenal ulcer and gastric carcinoma. The T cell response against H. pylori is thought to contribute to the pathogenesis of these diseases. Here, we show that mouse-adapted H. pylori is able to polyclonally act...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636676
更新日期:2007-07-01 00:00:00
abstract::Limited studies have addressed the ability of gammadelta T cells to become memory populations. We previously demonstrated that WC1.1(+) gammadelta T cells from ruminants vaccinated with killed Leptospira borgpetersenii proliferate and produce IFN-gamma in recall responses. Here we show that this response is dependent ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636216
更新日期:2007-05-01 00:00:00
abstract::Cyclosporin A (CsA), an immunosuppressive drug which completely suppresses the humoral response to thymus-dependent antigens, does not affect the generation of T helper (Th) cells during an in vitro stimulation of murine spleen cells with sheep red blood cells. The appearance of Th cells depends on time and their deve...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210304
更新日期:1991-03-01 00:00:00
abstract::The magnitude of the output of lymphocytes from Peyer's patches (PP), the morphology of the lymphocytes and their route of exit from PP has been examined in sheep. An extracorporeal perfusion system was used to selectively label a 3-4 m length of the terminal ileum of lambs at 6-10 weeks of age (the mesenteric lymph n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140103
更新日期:1984-01-01 00:00:00
abstract::A monoclonal antibody (mAb), 3A35, produced against mouse macrophages (M phi) was found to react against certain activated T cells. This mAb, a rat IgM, resulted from a cell fusion between a mouse plasmacytoma and rat lymphocytes immunized against mouse M phi. It bound more avidly to activated than to resident M phi. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170305
更新日期:1987-03-01 00:00:00
abstract::Expression of the complement-regulatory proteins (CRP) CD46, CD55 and CD59 represents a strategy used by tumor cells to evade complement-dependent cell cytoxicity stimulated by monoclonal antibodies. We have isolated two single-chain variable fragments (scFv) to CD55 and CD59 from a human phage-display library and fro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425920
更新日期:2005-07-01 00:00:00
abstract::MicroRNAs control developmental pathways and effector functions in immune cells. Previous studies have studied the role of microRNAs in natural killer (NK) cells. However, the mouse models of microRNA depletion used were nonNK-specific and only partially depleting, hampering the interpretation of the data obtained. To...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546163
更新日期:2016-08-01 00:00:00
abstract::We have previously reported the development of a two-step culture system in which soluble anti-mu monoclonal antibodies prime small resting murine B cells to secrete immunoglobulin (Ig) in response to restimulation with a mixture of interleukin-4 (IL-4) and IL-5. Here we have extended these studies to investigate the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230243
更新日期:1993-02-01 00:00:00
abstract::The analysis of an Ia specificity was performed in several H-2 haplotypes by evaluating the affinity of the binding to mouse spleen cells of a monoclonal anti-Ia alloantibody. We used the monoclonal antibody 10-2.16 (Oi et al., Curr. Top. Microbiol. Immunol. 1978. 81: 115.) which recognizes on I-A gene products a publ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110912
更新日期:1981-09-01 00:00:00
abstract::To study allelic exclusion of TcR genes we analyzed two types (I and II) of TcR beta transgenic mice. T cells derived from both types of mice contained similar amounts of transgenic RNA transcripts; however, surface expression of the transgenic beta chain was drastically reduced in type II compared to type I. In type ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200227
更新日期:1990-02-01 00:00:00
abstract::Major advances in preventing, delaying, or curing individual pathologies are responsible for an increasingly long life span in the developed parts of our planet, and indeed reaching eight to nine decades of life is nowadays extremely frequent. However, medical and sanitary advances have not prevented or delayed the un...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201546178
更新日期:2016-10-01 00:00:00
abstract::Recent studies have suggested that T cell memory for recall antigens resides in clones of primed T cells with a short inter-mitotic half-life. In humans such cells express an isoform of the leukocyte common antigen termed CD45RO. Nevertheless, little is known of the fate of these primed T cells after initial activatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240417
更新日期:1994-04-01 00:00:00
abstract::Changes in endothelial permeability are crucial in the pathogenesis of many diseases. Adenosine is one of the endogenous mediators controlling endothelial permeability under normal conditions, and an endothelial cell surface enzyme CD73 is a key regulator of adenosine production. Here we report that IFN-beta is a nove...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737793
更新日期:2007-12-01 00:00:00
abstract::Proliferation of BCL1 cells, generally used for the measurement of murine interleukin (IL)5, is shown to be strongly suppressed by interferon (IFN)-gamma. This effect can be abrogated by the addition of anti-IFN-gamma antibodies. Thus, the IL5 activity of culture supernatants derived from T cells simultaneously produc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190726
更新日期:1989-07-01 00:00:00
abstract::The germ-line V gene segments encoding the subgroups A and G of the BALB/c V kappa 21 family were cloned and assigned to the previously described 30-kb cluster of the V kappa 21 family. Sequence comparison revealed close homology between the two gene segments at the DNA and the predicted amino acid sequence level, ind...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191031
更新日期:1989-10-01 00:00:00
abstract::The present report describes functional characteristics of a monoclonal antibody (mAb), TA/211, which is reactive with a common determinant of the human CD45 molecules. When added to purified testing human T cells, TA/211, which is itself not mitogenic, leads to a strong proliferative response in combination with subm...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190226
更新日期:1989-02-01 00:00:00
abstract::Invariant NKT cells were stimulated with cholesteryl O-acyl α-glycosides in the context of CD1d. The activated NKT cells have potential to sustain the homeostasis in the body exposed to excess in either Th1- or Th2-immunity. ...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201847825
更新日期:2019-02-01 00:00:00
abstract::An anti-T cell receptor (TcR) monoclonal antibody (mAb), LC4, directed against a human leukemic T cell line, SUP-T13, caused DNA fragmentation ("apoptosis") and cell death upon binding to this cell line. Cross-linking of receptor molecules was necessary for this effect since F(ab')2, but not Fab', fragments of LC4 cou...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191023
更新日期:1989-10-01 00:00:00
abstract::DNase II in macrophages cleaves the DNA of engulfed apoptotic cells and of nuclei expelled from erythroid precursor cells. Macrophages in DNase II-deficient mice accumulate undigested DNA and constitutively produce IFN-beta as well as TNF-alpha. The IFN-beta causes severe anemia in the DNase II(-/-) embryos, which die...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838559
更新日期:2008-11-01 00:00:00
abstract::The murine T cell response to heterologous insulins provides a good model system for studying the mechanism of immune response (Ir)-gene function, since insulin is a small, chemically well-defined molecule. H-2b mice respond predominantly to A chain loop determinants of beef insulin, presented by the I-A epitope Ia. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830141208
更新日期:1984-12-01 00:00:00
abstract::When cytotoxic titrations were made of rabbit anti-mouse brain and anti-Thy-1.2 sera against mouse lymphoid cells in the presence of sodium barbital or xylocaine, it was observed that a proportion of T cells were much more sensitive (50 x) than the remainder. This effect could be detected with ethidium or fluorescein ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050408
更新日期:1975-04-01 00:00:00
abstract::Bcl-3 is an atypical member of the IκB family. Bcl-3 functions as a cofactor of p50/NF-κB1 or p52/NF-κB2 homodimers in nuclei, where it modulates NF-κB-regulated transcription in a context-dependent way. Bcl-3 has tumorigenic potential, is critical in host defense of pathogens, and has been reported to ameliorate or e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444994
更新日期:2015-04-01 00:00:00
abstract::The effect of Trypanosoma brucei infections on parasite-specific antibody responses was examined by immunization of infected mice with radio-attenuated trypanosomes of a noncross-reacting clone. Antibody titers (total Ig) against variant surface antigen were determined by indirect immunofluorescence. The suppression r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101216
更新日期:1980-12-01 00:00:00
abstract::The present study was designed to elucidate the role of Valpha14(+) NKT cells in the host defense against pulmonary infection with Streptococcus pneumoniae using Jalpha281 gene-disrupted mice (Jalpha281KO mice) that lacked this lymphocyte subset. In these mice, pneumococcal infection was severely exacerbated, as shown...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324254
更新日期:2003-12-01 00:00:00