Abstract:
:Dendritic cells (DC) are unique in their ability to present antigen to naive T cells, and therefore play a central role in the initiation of immune responses. Characterization of DC-specific genes may help to unravel the mechanism underlying their potent antigen presenting capacity. Here we describe the identification of a novel transcript, isolated by random sequencing of a cDNA library prepared from monocyte-derived DC, which we termed DC-specific transmembrane protein (DC-STAMP). DC-STAMP is specifically expressed by DC, and not in a panel of other leukocytes or non-hematopoietic cells. Interestingly, DC-STAMP was also detected in activated but not resting blood DC. The DC-STAMP transcript encodes a 470-amino acid protein containing seven putative transmembrane domains. Expression of a DC-STAMP-GFP fusion protein in 293 cells indicates that DC-STAMP is expressed at the cell surface, and has an intracellular C terminus. Surprisingly, no sequence homology was found with any other protein or multimembrane-spanning receptor. Therefore, we propose that DC-STAMP is a novel DC-specific multimembrane-spanning protein, representing a new group of transmembrane proteins.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Hartgers FC,Vissers JL,Looman MW,van Zoelen C,Huffine C,Figdor CG,Adema GJdoi
10.1002/1521-4141(200012)30:12<3585::AID-IMMU3585>keywords:
subject
Has Abstractpub_date
2000-12-01 00:00:00pages
3585-90issue
12eissn
0014-2980issn
1521-4141pii
10.1002/1521-4141(200012)30:12<3585::AID-IMMU3585>journal_volume
30pub_type
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