Abstract:
:The immunocytochemical characteristics of large granular lymphocytes (LGL), isolated from the liver sinusoids of euthymic and athymic (nude) rats, were investigated in electron microscopy by the immunoperoxidase technique. The LGL were found positive for MRC OX-8 (natural killer cells and cytotoxic/suppressor T cells) and negative for MRC OX-19 (pan-T marker) in both rat strains. The LGL were heterogeneous in the expression of the natural killer cell marker asialo-GM1 which was found on 56% of the LGL from euthymic and on 71% of the LGL from athymic rats. LGL were easily distinguished from the other cells in the preparations, "conventional" lymphocytes and monocytes, thanks to their highly characteristic ultrastructural features, in particular by the presence of specific electron-dense cytoplasmic granules and rod-cored vesicles. These features have been described formerly for the so-called "pit cells" and are more reliable than the classical LGL characteristics at the light microscopic level, i.e. the presence of azurophilic granules. Our results give further support for the existence of an important population of natural killer cells in the liver sinusoids.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Bouwens L,Wisse Edoi
10.1002/eji.1830171006subject
Has Abstractpub_date
1987-10-01 00:00:00pages
1423-8issue
10eissn
0014-2980issn
1521-4141journal_volume
17pub_type
杂志文章abstract::This study was performed as a means to examine the effects of environment and stem cell origin on the molding of T cell receptor gene rearrangements during T cell development. Lethally irradiated adult mice were first reconstituted with (C57BL/6 x DBA/2)F1 stem cells, which derived either from a fetal liver or adult b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211208
更新日期:1991-12-01 00:00:00
abstract::Adoptive lymphocyte transfers between Iga, Igb and Igd allotype-congenic mouse strains revealed host barriers against the production of certain donor allotypes. First, as recipients of Igb cells, Iga and Igd mice permitted the production of donor Ig-4b but not that of Ig-1 b. The apparent mediators of this Ig-1 b barr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830080805
更新日期:1978-08-01 00:00:00
abstract::Gradual depletion of CD4+ T cells is a typical characteristic of pathogenic SIV infection. Intriguingly, we find a spontaneous CD4+ T-cell homeostasis in northern pig-tailed macaques (Macaca leonina) during SIVmac239 infection. ...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201747284
更新日期:2018-02-01 00:00:00
abstract::The purpose of this work was to study the interrelationship of proliferation and secretion of plasminogen activator (PA) and interferon (IFN) by murine macrophages. For induction of macrophage proliferation and secretion of PA, concanavalin A (Con A) was used. Secretion of IFN was induced by polyinosinic polycytidylic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130210
更新日期:1983-02-01 00:00:00
abstract::Cells from the monoclonal B cell lymphoma I.29 expressing surface IgM (mu +) are capable of differentiating in vitro to IgM secretion and of switching to IgA or IgE production in response to lipopolysaccharide (LPS) stimulation. To determine whether a single mu + B cell is capable of undertaking both differentiative p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170419
更新日期:1987-04-01 00:00:00
abstract::Cholesterol- and sphingolipid-rich membrane microdomains termed lipid rafts appear to play a central role in B cell activation. In mature B cells, signaling through the B cell antigen receptor(BCR) is initiated from within rafts and leads to activation. In immature B cells, the BCR is excluded from rafts and signaling...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200112)31:12<3789::aid-immu3789>
更新日期:2001-12-01 00:00:00
abstract::To detect possible differences in immunogenicity between tumors induced in T cell-deficient mice and phenotypically normal congenic mice, 16 sarcomas, 8 having developed in nude BALB/c mice and 8 having developed in congenic normal (nu/+) mice, were transplanted to normal BALB/c recipients and the rates of rejection o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260827
更新日期:1996-08-01 00:00:00
abstract::DNase II in macrophages cleaves the DNA of engulfed apoptotic cells and of nuclei expelled from erythroid precursor cells. Macrophages in DNase II-deficient mice accumulate undigested DNA and constitutively produce IFN-beta as well as TNF-alpha. The IFN-beta causes severe anemia in the DNase II(-/-) embryos, which die...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838559
更新日期:2008-11-01 00:00:00
abstract::The thymus provides the microenvironment in which thymocytes develop into mature T cells, and interactions with thymic stromal cells are thought to provide the necessary signals for thymocyte maturation. Recognition of self-MHC by T cells is a basic requirement for mature T-cell functions, and those thymocytes that do...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201142338
更新日期:2012-07-01 00:00:00
abstract::Current models of lymphocyte traffic suggest that homing specificities of T cells to tissues such as skin are generated outside the thymus as a result of activation of naive T cells by antigen in lymph nodes. Virgin T cells are thought to home to high endothelial venules in lymph nodes, but are thought to be unable to...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250314
更新日期:1995-03-01 00:00:00
abstract::Psoriasis is an inflammatory disease of the skin affecting 2-3% of the population, characterized by a thickening of the epidermis and immune infiltrates throughout the dermis and epidermis, causing skin lesions that can seriously affect quality of life. The study of psoriasis has historically been hampered by the lack...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343801
更新日期:2013-12-01 00:00:00
abstract::NK cells can kill transformed, infected and stressed cells while most normal cells are spared. NK cells are activated by various endogenous self-ligands, some of which are actually expressed by normal cells. Thus, NK cells are inherently self-reactive and consequently, potentially auto-aggressive. How these cells are ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200838755
更新日期:2008-11-01 00:00:00
abstract::The stimulatory as well as the inhibitory capacity of alloantisera has been investigated with respect to rat mast cell functions. Alloantibody against alloantigens coded for by the major histocompatibility (H-1) gene region promoted histamine release from purified LEW mast cells. This process was found to be complemen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060813
更新日期:1976-08-01 00:00:00
abstract::CD11a/CD18 (beta2)-integrins are expressed on leukocytes and are involved in cell adhesion and signaling. Despite extensive studies the signaling pathways and molecular mechanisms involved in integrin regulation in T cells remain not completely understood. We have now studied the involvement of the tyrosine kinase Lck...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1670::AID-IMMU1670>3
更新日期:2002-06-01 00:00:00
abstract::Polymorphonuclear neutrophils (PMN) modulate the adaptive immune response through interactions with immature dendritic cells (iDC) while spontaneous apoptotic neutrophils PMNapo (PMNapo) may have an inhibitory effect on DC functions. We investigate the effect exerted by PMNapo in DC maturation and the role of Mycobact...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636771
更新日期:2007-06-01 00:00:00
abstract::MHC class II tetramers are attractive tools to study antigen-specific CD4(+) T cell responses in various clinical situations in humans. HLA-DRA1*0101/DRB1*0401 MHC class II heterodimers were produced as empty molecules using the Drosophila melanogaster expression system. Peptide binding experiments revealed that these...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425281
更新日期:2004-10-01 00:00:00
abstract::IL-10, produced by a wide variety of cells, is a highly pleiotropic cytokine that plays a critical role in the control of immune responses. However, its regulatory activity in tumor immunity remains poorly understood. In this study, we report that IL-10 deficiency robustly suppressed the formation of malignant pleural...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048574
更新日期:2020-11-01 00:00:00
abstract::Cellular metabolism is dynamically regulated in NK cells and strongly influences their responses. Metabolic dysfunction is linked to defective NK cell responses in diseases such as obesity and cancer. The transcription factors, sterol regulatory element binding protein (SREBP) and cMyc, are crucial for controlling NK ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048784
更新日期:2020-09-18 00:00:00
abstract::Natural killer (NK) cell activity can be stimulated by different surface receptors. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating human NK cell cytotoxicity and cytokine production. Here we show that stimulation of human NK cells by anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636146
更新日期:2006-12-01 00:00:00
abstract::The delivery of T-cell help to B cells is antigen-specific, MHC-restricted, and CD40L (CD154) dependent. It has been thought that when a T cell recognizes an antigen-presenting B cell, CD40L expressed on the T-cell surface engages with CD40 on the surface of B cells as long as the cells remain conjugated. By adding fl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646504
更新日期:2017-01-01 00:00:00
abstract::The aim of this study was to investigate the effects of IL-17-producing T cells, including Th17 and Tc17 cells, on acute graft-versus-host disease (aGVHD) in patients who had undergone granulocyte colony-stimulating factor (G-CSF)-mobilised peripheral blood progenitor cell (PBPC) and G-CSF-primed bone marrow (G-BM) tr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040793
更新日期:2011-02-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated autoimmune disease inducible in rodents by immunization of brain-specific antigens such as myelin basic protein (MBP). It is also well known that various strains of rats differ in their susceptibility to EAE upon active immunization. To elucidate the...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200934
更新日期:1990-09-01 00:00:00
abstract::Plaque-forming cell (PFC) assays were used to investigate in vitro the immunoregulatory mechanism operating in the self-limiting anti-glomerular basement membrane (GBM) autoantibody response of Brown Norway (BN) rats given HgCl2. The peak splenic PFC response occurred at day 9; thereafter the response fell sharply and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140516
更新日期:1984-05-01 00:00:00
abstract::The influence of Th2 cytokines in tuberculosis has been a matter of dispute. Here we report that IL-4 has a profound regulatory effect on the infection of BALB/c mice with Mycobacterium tuberculosis. Depletion of IL-4 with a neutralizing mAb caused only evanescent reduction of lung infection, but when combined with i....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636764
更新日期:2007-03-01 00:00:00
abstract::There is a current need for safe, cheap, and effective vaccine adjuvants, to combine with sub-unit antigens to enhance their immunogenicity. In this study we have used probiotic Bacillus subtilis spores, known to be safe and fully tolerated by ingestion in man, and explored their ability to influence the magnitude and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636875
更新日期:2007-06-01 00:00:00
abstract::The atypical chemokine receptor CXCR7 binds the chemokines CXCL12 and CXCL11. The receptor is widely expressed and was shown to tune CXCR12-induced responses of CXCR4. Here, the function of CXCR7 was examined at late stages of human B-cell maturation, when B cells differentiate into Ab-secreting plasmablasts. We ident...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343907
更新日期:2014-03-01 00:00:00
abstract::Mature T cells may be produced in the thymus, or by expansion in the periphery. While thymus output of virgin cells ensures repertoire diversity, peripheral expansion increases the size of rare clones, and thus the efficiency of immune responses. We studied the role of both phenomena in the generation of the CD8+ T ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250802
更新日期:1995-08-01 00:00:00
abstract::The TCR structure of T cell hybridomas recognizing a tumor glycan-defined epitope has been studied using reverse transcriptase-PCR and gene sequencing. The hybridomas had been raised against a glycopeptide, T72(Tn), consisting of the mouse hemoglobin-derived decapeptide Hb(67 - 76), O-glycoslated in position 72 with a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199909)29:09<2759::AID-IMM
更新日期:1999-09-01 00:00:00
abstract::The migration of monocytes to sites of inflammation is largely determined by their response to chemokines. Although the chemokine specificities and expression patterns of chemokine receptors are well defined, it is still a matter of debate how cells integrate the messages provided by different chemokines that are conc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838906
更新日期:2009-04-01 00:00:00
abstract::Major histocompatibility complex (MHC) class II-associated antigen presentation is mainly linked to processing of exogenous antigens upon cellular uptake by endocytosis, but has also been observed for endogenously synthesized antigens. We have studied the MHC class II-associated presentation of the endogenously synthe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251230
更新日期:1995-12-01 00:00:00