Abstract:
:MHC class II tetramers are attractive tools to study antigen-specific CD4(+) T cell responses in various clinical situations in humans. HLA-DRA1*0101/DRB1*0401 MHC class II heterodimers were produced as empty molecules using the Drosophila melanogaster expression system. Peptide binding experiments revealed that these molecules could be loaded efficiently with appropriate MHC class II tumor epitopes. Interestingly, MHC class II tetramer staining was influenced by modifications in membrane lipid rafts, and could in itself induce activation changes of stained CD4(+) T cells at 37 degrees C. In order to increase the threshold of detection of poorly represented peripheral antigen-specific CD4(+) T cells, we combined cell sorting using MHC class II multimer beads together with TCR analysis using the immunoscope technology. This strategy greatly increased the sensitivity of detection of specific CD4(+) T cells to frequencies as low as 4 x 10(-6) among peripheral blood mononuclear cells. Such a combined approach may have promising applications in the immunomonitoring of patients under vaccination protocols to tightly follow induced antigen-specific CD4(+) T cells expressing previously identified TCR.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Lemaître F,Viguier M,Cho MS,Fourneau JM,Maillère B,Kourilsky P,Van Endert PM,Ferradini Ldoi
10.1002/eji.200425281keywords:
subject
Has Abstractpub_date
2004-10-01 00:00:00pages
2941-9issue
10eissn
0014-2980issn
1521-4141journal_volume
34pub_type
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