Abstract:
:By comparing the restriction patterns of the DNA from 23 unrelated individuals 16 polymorphisms were defined which allowed us to differentiate between the duplicated copies Op, Ap, Lp and Od, Ad, Ld of the kappa locus (p for the C kappa proximal, d for the distal copy). Some of these duplication-differentiating polymorphisms or DDP revealed also allelic differences between individuals; they are therefore restriction fragment length polymorphism (RFLP) markers at the same time. Three RFLP in the single copy B-J kappa-C kappa region were included into the study. Three basic haplotypes were derived from the combined genotype data, haplotypes N, G and 11. The latter haplotype in which the whole distal copy of the kappa locus is missing was found three times among the 46 haploid genomes studied. The genotypes of the family members of an individual who is homozygous for haplotype 11 are consistent with Mendelian inheritance. Haplotypes N and G are distinguished from each other by eight RFLP markers. Six additional haplotypes, which were found in one or several individuals each, can be derived from the basic haplotypes N and G by hypothetical recombination and/or mutation events.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Pargent W,Schäble KF,Zachau HGdoi
10.1002/eji.1830210808subject
Has Abstractpub_date
1991-08-01 00:00:00pages
1829-35issue
8eissn
0014-2980issn
1521-4141journal_volume
21pub_type
杂志文章abstract::It has been reported that the 45-kDa low-affinity Fc epsilon receptor (Fc epsilon RII) on B cells is cleaved spontaneously from the cell surface to release soluble fragments. This study demonstrates an additional fate of the Fc epsilon RII. 125I-labeled CD23+ B cells were cultured for 24 h at 37 degrees C. After lysis...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220634
更新日期:1992-06-01 00:00:00
abstract::High BDCA3 expression is associated with a specific human IFN-λ-producing dendritic cell (DC) subset. However, BDCA3 has also been detected on other DC subsets. Thus far, development and function of BDCA3 expression on DCs remains poorly understood. Human Langerhans cells (LCs) and interstitial DCs (intDCs) can be gen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444802
更新日期:2015-05-01 00:00:00
abstract::The stimulatory as well as the inhibitory capacity of alloantisera has been investigated with respect to rat mast cell functions. Alloantibody against alloantigens coded for by the major histocompatibility (H-1) gene region promoted histamine release from purified LEW mast cells. This process was found to be complemen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060813
更新日期:1976-08-01 00:00:00
abstract::We have utilized the sensitive polymerase chain reaction (PCR) to determine whether cytotoxic T lymphocyte (CTL) hybridomas generated from peritoneal exudate lymphocytes (PEL) and mixed lymphocyte cultures (MLC) express transcripts for perforin and the cytotoxic cell proteinases CCP1 to CCP5. We could readily detect l...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221225
更新日期:1992-12-01 00:00:00
abstract::Among 15 anti-DNA antibody-producing hybridomas derived from a single NZB X NZW F1 mouse, an IgM and an IgG were shown to use the same VH gene of the Q52 family. Using a combination of two primers both consisting of a mixture of oligonucleotides (one complementary to the 5' end of VH segment and one to the 3' end of V...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220417
更新日期:1992-04-01 00:00:00
abstract::Cartilage-specific proteins are considered potential autoantigens that could continuously fuel autoimmune responses directed to the joints in rheumatoid arthritis (RA). Using recombinant chimeric collagen type II we have identified one major type II collagen (CII) epitope (denoted U1) recognized by RA sera. The U1 epi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200106)31:6<1666::aid-immu1666>3
更新日期:2001-06-01 00:00:00
abstract::Interleukin-18 (IL-18) is not only a co-stimulus for the induction of interferon-gamma but also has direct proinflammatory effects by inducing tumor necrosis factor-alpha (TNF-alpha), IL-1, IL-8 and IL-6. However, the cascade of events leading to induction of cytokines by IL-18 is unclear. The aim of the present study...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200010)30:10<3057::AID-IMMU3057>
更新日期:2000-10-01 00:00:00
abstract::B cell development is dependent on both direct interactions with stromal cells and their secreted cytokines. The precise mechanisms by which these interactions regulate B cell differentiation are currently unknown. We report here that a novel growth factor thymic stromal-derived lymphopoietin (TSLP) can replace the ac...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260103
更新日期:1996-01-01 00:00:00
abstract::We have shown previously that complement factor H (CFH) and complement factor B (CFB) are constitutively expressed by retinal pigment epithelial cells and their production is regulated by inflammatory cytokines, suggesting that the alternative pathway (AP) of complement activation might play a role in retinal inflamma...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040323
更新日期:2010-10-01 00:00:00
abstract::Tissue factor (TF) is a transmembrane glycoprotein that plays an essential role in hemostasis by activating coagulation. TF is also expressed by monocytes/macrophages as part of the innate immune response to infections. In the current study, we determined the role of TF expressed by myeloid cells during Mycobacterium ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545817
更新日期:2016-02-01 00:00:00
abstract::ME3738 is a new compound that attenuates liver disease in several models of acute and chronic liver inflammation. We used the concanavalin A (Con A) model to elucidate the molecular mechanisms of ME3738 to block liver cell damage. Pretreatment of BALB/c mice with ME3738 prior to Con A injection resulted in a significa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323651
更新日期:2003-08-01 00:00:00
abstract::It has been reported that Thy-1+CD3+CD4-CD8- cells as well as Langerhans cells migrate from organ-cultured murine skin into culture medium. We examined whether these Thy-1+ populations of migrating cells were derived from Thy-1+ dendritic epidermal T cells (Thy-1+ DEC) and found that they were Thy-1+CD3+CD4-CD8-gamma/...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230746
更新日期:1993-07-01 00:00:00
abstract::In the thymus, in order to become MHC-restricted self-tolerant T cells, developing thymocytes need to interact with cortical and medullary thymic epithelial cells (TECs). Although the presence of a common bipotent progenitor for these functionally and structurally distinct epithelial subsets has been clearly establish...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201343323
更新日期:2013-03-01 00:00:00
abstract::IL-22 is an IFN-IL-10 cytokine family member, which is produced by activated Th1 and NK cells and acts primarily on epithelial cells. Here we demonstrate that IL-22, in contrast to its relative IFN-gamma, regulates the expression of only a few genes in keratinocytes. This is due to varied signal transduction. Gene exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535503
更新日期:2006-05-01 00:00:00
abstract::Deoxyguanosine (dGuo) has been implicated as the toxic metabolite causing a severe impairment of cellular immunity in children with a genetic deficiency of purine nucleoside phosphorylase (PNP). In peripheral blood T cells of normal donors both the pathway which leads to phosphorylation of dGuo (ultimately resulting i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160412
更新日期:1986-04-01 00:00:00
abstract::Regulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we character...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747041
更新日期:2017-12-01 00:00:00
abstract::Inflammatory bowel disease (IBD) is characterized by a dysregulated intestinal immune response with elevated levels of the Th1 cytokines TNF, IL-12 and IFN-gamma. The luminal flora has been implicated as a major factor contributing to the initiation and perpetuation of chronic intestinal inflammation by as yet unknown...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200207)32:7<2084::AID-IMMU2084>3
更新日期:2002-07-01 00:00:00
abstract::Adenosine monophosphate-activated protein kinase (AMPK) is a serine/threonine kinase and is crucial for cellular energy homeostasis. The exact role of AMPK during memory CD8(+) T-cell differentiation, a process that changes from the metabolically active state of effector T cells to one of quiescence in memory cells is...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201343483
更新日期:2013-04-01 00:00:00
abstract::A panel of closely and distantly related lysozymes and lysozyme-peptide fragments were utilized in assessing the specificity repertoire of murine anti-hen egg white lysozyme hybridomas. The 44 monoclonal antibodies could be divided into a minimum of 18 fine specificity groups in tests using the lysozyme panel. Two hyb...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140116
更新日期:1984-01-01 00:00:00
abstract::Injection of incomplete Freund's adjuvant (IFA) into the footpads of BALB/c mice induced an acute inflammation. Draining popliteal lymph nodes showed major histocompatibility complex (MHC) class II-restricted proliferation when challenged in vitro with recombinant Mycobacterium bovis 65-kDa heat shock protein (hsp65)....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230107
更新日期:1993-01-01 00:00:00
abstract::By stimulating peripheral blood mononuclear cells of four healthy donors with a mixture of overlapping peptides representing the core domain of p53, we established two CD4+ alphabeta T cell clones and four lines that recognized wild-type and mutant p53 proteins as well as p53 self peptides in an HLA class II-restricte...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199801)28:01<305::AID-IMMU
更新日期:1998-01-01 00:00:00
abstract::HSP gp96-based vaccines have been trialled in rodent models and, more recently, in humans. Better understanding of gp96's immunomodulatory role will help with the design of more effective strategies for treatment of cancer and infectious diseases. In this study, we monitored the activities of T cells and activation of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939593
更新日期:2009-11-01 00:00:00
abstract::The activity of granzyme B, a main effector molecule of cytotoxic T lymphocytes (CTL) and natural killer cells, is regulated by the human intracellular serpin proteinase inhibitor 9 (PI9). This inhibitor is particularly expressed by CTL and dendritic cells, in which it serves to protect these cells against endogenous ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425949
更新日期:2005-04-01 00:00:00
abstract::Immunotherapies that augment antitumor T cells have had recent success for treating patients with cancer. Here we examined whether tumor-specific CD4(+) T cells enhance CD8(+) T-cell adoptive immunotherapy in a lymphopenic environment. Our model employed physiological doses of tyrosinase-related protein 1-specific CD4...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343718
更新日期:2014-01-01 00:00:00
abstract::Regulation of the development of thymocytes into mature T cells within the thymus is now known to involve antigen-induced deletion, by apoptosis, of potentially autoreactive thymocytes, and it can be mimicked either by stimulating the T cell receptor (TcR) complex by monoclonal antibody (mAb) or by ionophore-induced e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250706
更新日期:1995-07-01 00:00:00
abstract::The requirement for cell interactions in T cell activation has been studied with two continuously in vitro growing T cell clones. These clones are specific for minor histocompatibility antigens, are H-2K restricted, and one clone is functionally a cytolytic T lymphocyte. Both can proliferate when interleukin 2 is adde...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830141011
更新日期:1984-10-01 00:00:00
abstract::Changes in the epigenetic landscape of immune cells are a crucial component of gene activation during the induction of inflammatory responses, therefore it has been hypothesized that epigenetic modulation could be employed to restore homeostasis in inflammatory scenarios. Fungal pathogens cause a large burden of morbi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848081
更新日期:2019-11-01 00:00:00
abstract::The covalent binding reaction of the third component of complement (C3) with rabbit IgG immune aggregates has been studied by enzymic digestion of C3b-IgG adducts. In these adducts C3b was radioactively labeled in the free thiol group generated during activation of the internal thioester of C3. Trypsin digestion of 14...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240316
更新日期:1994-03-01 00:00:00
abstract::T helper cell type 2 (Th2) cells when triggered by antibodies to CD3 acquire the capacity to stimulate the polyclonal proliferation of syngeneic, resting B cells. Here, we tested the ability of various monoclonal antibodies (mAb) to block the B cell proliferation-inducing potential of such activated Th2 cells. We demo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220246
更新日期:1992-02-01 00:00:00
abstract::Human T cells expressing Vgamma9Ndelta2-encoded TCR recognize several nonpeptide phosphoantigens in the absence of major histocompatibility complex restriction. As these cells respond differentially to increasing concentrations of structurally related phosphoantigens, such ligands constitute agonists of different stre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200105)31:5<1628::AID-IMMU1628>3
更新日期:2001-05-01 00:00:00