Abstract:
:Cartilage-specific proteins are considered potential autoantigens that could continuously fuel autoimmune responses directed to the joints in rheumatoid arthritis (RA). Using recombinant chimeric collagen type II we have identified one major type II collagen (CII) epitope (denoted U1) recognized by RA sera. The U1 epitope is a triple helical structure formed by 11 amino acids (triple helical position 494-504) and colocalizes with the recently described alpha1beta1/alpha2beta1 integrin binding site. It is a major epitope, found in 14/22 RA sera positive for antibodies to CII. One individual could be followed for a long time and the results showed that IgG antibodies specific for the U1 epitope were maintained along the chronic disease course but suppressed during periods of cyclosporin A and anti-CD4 treatment. We also found that the U1 epitope was recognized in rats susceptible to collagen-induced arthritis. A monoclonal autoantibody (mAb 126.30) was raised from DA rats, which bound the same epitope. The antibodies bound the cartilage in vivo showing that the epitope is exposed to the immune system for immune complex formation in the intact joint.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Kraetsch HG,Unger C,Wernhoff P,Schneider C,Kalden JR,Holmdahl R,Burkhardt Hdoi
10.1002/1521-4141(200106)31:6<1666::aid-immu1666>3keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
1666-73issue
6eissn
0014-2980issn
1521-4141pii
10.1002/1521-4141(200106)31:6<1666::AID-IMMU1666>3journal_volume
31pub_type
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