Abstract:
:The infiltration and activation of monocytes is a hallmark of chronic inflammation, including that associated with a variety of disease states such as rheumatoid arthritis, atherosclerosis, and various autoimmune conditions. Recently, a family of small molecular mass proteins has been described which appear to have inflammatory properties, including chemoattractant effects on monocytes. We report here on the molecular cloning, characterization, and functional expression of mu RANTES, a new murine member of this family. mu RANTES expressed in a mammalian expression system is an approximately 8-kDa protein exhibiting immune cross-reactivity with a rabbit polyclonal antiserum generated against human RANTES. Boyden chamber chemotaxis experiments reveal some lack of species specificity in monocyte chemoattractant potential, as recombinant mu RANTES attracts human monocytes in a dose-dependent fashion in vitro. mu RANTES and its human homolog share approximately 85% amino acid identity, a higher level of conservation than that seen with any other species homologs in this cytokine family, and second only to transforming growth factor-beta among reported immune cytokines.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Schall TJ,Simpson NJ,Mak JYdoi
10.1002/eji.1830220621keywords:
subject
Has Abstractpub_date
1992-06-01 00:00:00pages
1477-81issue
6eissn
0014-2980issn
1521-4141journal_volume
22pub_type
杂志文章abstract::The immunosuppressive drugs FK506 and rapamycin bind to a family of intracellular proteins termed FK506-binding proteins (FKBP). FK506 and rapamycin inhibit lymphocyte-activation pathways by forming complexes with an FKBP; subsequently, the drug/FKBP complexes interact with target molecules involved in signal transduc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250239
更新日期:1995-02-01 00:00:00
abstract::We have investigated perforin and granzyme B expression in graft-infiltrating lymphocytes of patients who underwent heart transplantation. Those proteins are commonly present in the cytoplasmic granules of cytotoxic T lymphocytes and are released upon effector-target cell interaction. From 28 patients 103 endomyocardi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240136
更新日期:1994-01-01 00:00:00
abstract::CD25+ FOXP3+CD4+ T cells (Treg) have been considered to play an important role in immune tolerance against several tumor antigens. It has also been indicated that high-level expression of FOXP3 (FOXP3high) is sufficient to confer suppressive activity to normal non-Treg. Here, we showed for the first time that vascular...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939887
更新日期:2010-01-01 00:00:00
abstract::CD72 has been reported to regulate BCR-mediated signals both positively and negatively. SHP-1 and Grb2 bind, respectively, to ITIM1 and ITIM2 of CD72. We generated transformed B cell lines with an immature phenotype following J2 virus infection of splenocytes from CD72(-/-) and wild-type (Wt) mice. The transformed lin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425775
更新日期:2005-05-01 00:00:00
abstract::Unexpected fetal loss is one of the common complications of pregnancy; however, the pathogenesis of many miscarriages, particularly those not associated with infections, is unknown. We previously found that activated DEC-205+ dendritic cells (DCs) and NK1.1+ invariant natural killer T (iNKT) cells are recruited into t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747162
更新日期:2018-06-01 00:00:00
abstract::CD30 is a costimulatory molecule of the TNF receptor superfamily, expressed on activated T and B cells. Previously, we have shown in a murine asthma model the crucial role of CD30 signaling for the development of this Th2-cell-mediated disease. In the present study, we investigated the role of CD30 in the maintenance ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839004
更新日期:2009-07-01 00:00:00
abstract::Incomplete allelic exclusion of TCRa gene rearrangement permits the generation of dual Valpha T cells, though the issues of their frequency and whether both alphabeta pairs participate in thymic selection have not been resolved. Both questions have been investigated using lymphocytes from mice hemizygous at the TCRa l...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199807)28:07<2115::AID-IMM
更新日期:1998-07-01 00:00:00
abstract::Influenza matrix peptide 58-66 is shown to be the optimal nonamer for binding to HLA-A2 and presentation to cytotoxic T lymphocytes (CTL). If titered out to 2 x 10(-10) - 4 x 10(-10) M in CTL-mediated lysis assays and to 3 x 10(-9) M in an HLA-A2 assembly-stabilization assay in cell lysates. The peptide was shown to m...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220404
更新日期:1992-04-01 00:00:00
abstract::An experimental model of adenosine deaminase deficiency was established on the human T cell line Jurkat by using 2'-deoxycoformycin, a strong specific inhibitor of the enzyme. When deoxyadenosine was added to the inhibited cells, the nucleotide profile was modified reproducing that found in lymphocytes from adenosine ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200322
更新日期:1990-03-01 00:00:00
abstract::Mice deficient for the adapter protein SLP65 (BLNK) show a partial block in early B cell development, reduced numbers of mature B cells in the periphery, an absence of B1 cells and a reduction of IgM and IgG3 serum immunoglobulin levels. A strikingly similar phenotype is observed in Btk-deficient mice. To investigate ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<2164::aid-immu2164>3
更新日期:2001-07-01 00:00:00
abstract::Local graft-vs.-host reaction (GVHR) was induced in rats by injecting parental cells into young F1 recipients. As a consequence of antigenic stimulation in the course of developing GVHR in the responding lymph nodes, the number of interleukin 2-receptor (IL 2R)-bearing T cells increased from less than 1% up to 10% of ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161020
更新日期:1986-10-01 00:00:00
abstract::Maintenance of homeostasis at body barriers that are constantly challenged by microbes, toxins and potentially bioactive (macro)molecules requires complex, highly orchestrated mechanisms of protection. Recent discoveries in respiratory research have shed light on the unprecedented role of airway epithelial cells (AEC)...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201847811
更新日期:2020-05-01 00:00:00
abstract::AMPK (adenosine monophosphate-activated protein kinase) is phosphorylated (AMPK-P) in response to low energy through allosteric activation by Adenosine mono- or diphosphate (AMP/ADP). Folliculin (FLCN) and the FLCN-interacting proteins 1 and 2 (FNIP1, 2) modulate AMPK. FNIP1 deficiency patients have a AMPK-P gain of f...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201948504
更新日期:2020-07-01 00:00:00
abstract::Cell ablation is a valuable complement to mutagenesis for experimentally defining specific cell functions in physiology and pathophysiology in small animal models. One of the most popular ablation strategies involves transgenic expression of a primate diphtheria toxin receptor (DTR) on murine cells that are otherwise ...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201847527
更新日期:2018-07-01 00:00:00
abstract::T lymphocytes can be activated and induced to proliferate through stimulation of the CD2 glycoprotein with functional combinations of CD2 antibodies. However, this mechanism of signal transduction via CD2 is still not fully understood. We have investigated which molecules on the T cell surface preferentially associate...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200205)32:5<1509::AID-IMMU1509>3
更新日期:2002-05-01 00:00:00
abstract::Current concepts on the pathogenesis of chronic asthma emphasize the role of several inflammatory cell populations and their respective mediators that interact in a complex network. However, beside inflammatory cells, lymphocytes are also present in asthmatic airways. Although little is known about their involvement i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230404
更新日期:1993-04-01 00:00:00
abstract::In this report, we provide new evidence of a crosstalk between T cell activation and adhesion processes through a functional cytokeleton. We show that CD82 signaling induces long-lasting adhesion, spreading and development of membrane extensions, involving actin polymerization. Addition of various co-stimuli (phorbol ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199812)28:12<4332::AID-IMM
更新日期:1998-12-01 00:00:00
abstract::This report describes the study of the expression of an idiotype in the human population which is associated with antibodies to N-acetyl-D-glucosamine (GlcNAc) present in most human sera presumably due to streptococcal infections. The idiotype is identified with antisera and monoclonal antibodies prepared against the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130402
更新日期:1983-04-01 00:00:00
abstract::A detailed restriction map of a 430-kb contig comprising the single Ckappa, the 5 Jkappa and the adjoining 22 Vkappa gene segments is presented. The first 12 Vkappa genes following the JkappaCkappa region belong to the Vkappa21 family, the subsequent ones to the closely related families Vkappa8 and Vkappal 9/ 28. Prev...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199805)28:05<1458::AID-IMM
更新日期:1998-05-01 00:00:00
abstract::Epidermal keratinocytes differentiate and form a multilayered epidermis, which is the primary barrier between the body and the outer environment. As the epidermis is constantly exposed to a variety of microbial pathogens, its function of resisting microbial pathogens is vital. This characteristic feature is formed dur...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425827
更新日期:2005-06-01 00:00:00
abstract::IL-7 is a major regulator of lymphocyte homeostasis; however, little is known about the mechanisms that regulate IL-7 production. To study Il7 gene regulation in vivo, we generated a novel IL-7-reporter mouse, which allows the non-invasive quantification of Il7 gene activity in live mice and, additionally, the simulta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040441
更新日期:2010-09-01 00:00:00
abstract::We have developed a long-term culture system using the murine bone marrow stromal cells MS-5 to support the growth of progenitor B cells with CD34-, CD10+, CD19+, and cytoplasmic mu chain (C mu)-negative surface phenotype from human CD34+ cells purified from umbilical cord blood (CB). When 10(3) CD34+ cells/well were ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<855::AID-IMMU
更新日期:1998-03-01 00:00:00
abstract::T-cell activation and the subsequent transformation of activated T cells into T-cell blasts require profound changes in cell volume. However, the impact of cell volume regulation for T-cell immunology has not been characterized. Here we studied the role of the cell-volume regulating osmolyte transporter Taut for T-cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141690
更新日期:2012-04-01 00:00:00
abstract::In the past 15 years, it became clear that the symbiotic microbiota has an important impact on the development and regulation of the immune system. Consequently, it is incorrect to interpret a phenotype solely as a direct result of the genotype, without considering the impact of the microbiota. In fact, ignorance of t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048615
更新日期:2020-06-01 00:00:00
abstract::Cycling lymphocytes may express the enzyme telomerase which is involved in maintenance of telomere length and cell proliferation potential. In CD8(+) T cells freshly isolated from peripheral blood, we found that in vivo cycling cells expressed HLA-DR. Furthermore, CD28-positive cells are known to have longer telomeres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200102)31:2<459::aid-immu459>3.0
更新日期:2001-02-01 00:00:00
abstract::B cells isolated from germinal centers (GC) of immune mice 2-5 days after antigen (Ag) challenge migrate in response to chemotactic signals, whereas GC B cells isolated at other times and resting B cells do not. Since B cells are in direct contact with follicular dendritic cells (FDC) in GC we reasoned that FDC might ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250437
更新日期:1995-04-01 00:00:00
abstract::A hallmark of parasite infection is the accumulation of innate immune cells, notably granulocytes and mast cells, at the site of infection. While this is typically viewed as a transient response, with the tissue returning to steady state once the infection is cleared, we found that mast cells accumulated in the large-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646738
更新日期:2017-02-01 00:00:00
abstract::The active role of chemokines in the central nervous system (CNS) during the pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been clearly established. In this study the expression pattern of several chemokines and cytokines was elucidated using reverse transcription-PCR and immunohistochemistry in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1911::AID-IMMU1911>3
更新日期:2000-07-01 00:00:00
abstract::Missing-self-reactivity can be mimicked by blocking self-specific inhibitory receptors on NK cells, leading to increased rejection of syngeneic tumor cells. Using a mouse model, we investigated whether Ab-mediated blocking of inhibitory receptors, to a degree where NK cells rejected syngeneic tumor cells, would still ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939755
更新日期:2010-03-01 00:00:00
abstract::CBA/J mice immunized with thyroglobulin (Tg) develop an experimental autoimmune thyroiditis (EAT) with lymphocytic infiltration of the thyroid glands, autoantibodies to Tg and occurrence of EAT-specific T cells. When these mice were treated for 4 weeks after immunization with 1 mg/week of a monoclonal antibody (mAb) t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230143
更新日期:1993-01-01 00:00:00