Abstract:
:Cell ablation is a valuable complement to mutagenesis for experimentally defining specific cell functions in physiology and pathophysiology in small animal models. One of the most popular ablation strategies involves transgenic expression of a primate diphtheria toxin receptor (DTR) on murine cells that are otherwise resistant to the bacterial exotoxin. The efforts of many laboratories using the DTR approach over the years have yielded numerous valuable insights into specific cell functions. Here, we will discuss the technical aspects of the DTR approach, including the strengths, pitfalls, and future strategies to overcome the shortcomings, highlighting a recent paper published in the European Journal of Immunology [El Hachem et al. Eur. J. Immunol. 2018 https://doi.org/10.1002/eji.201747351]. A particular focus will be given to the application of DTR approach to decipher in vivo functions of the murine myeloid cell compartment.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Ruedl C,Jung Sdoi
10.1002/eji.201847527subject
Has Abstractpub_date
2018-07-01 00:00:00pages
1114-1119issue
7eissn
0014-2980issn
1521-4141journal_volume
48pub_type
评论,杂志文章abstract::Resistance and susceptibility to the intestinal nematode Trichuris muris has been shown to be dependent upon the induction of T helper type 2 (Th2) or Th1 cells, respectively. This study demonstrates that in a normally resistant strain of mouse, i.e. BALB/K which mounts a dominant Th2 response, sub-threshold levels of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241230
更新日期:1994-12-01 00:00:00
abstract::We have fractionated human tonsillar B cells on the basis of CD5 expression and determined the nucleotide sequences of immunoglobin light chain variable (V) regions encoded by the single member of the V chi 4 gene family in both CD5+ and CD5- populations. The majority of cDNA from both CD5+ and CD5- B cells population...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230636
更新日期:1993-06-01 00:00:00
abstract::Viruses encode immune evasion mechanisms to survive in the immunocompetent host. Chemokines mediate the migration of immune cells and their critical role in immunity is emphasized by the numerous virus-encoded strategies to modulate their activity. Evidence published in this issue of the European Journal of Immunology...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737215
更新日期:2007-04-01 00:00:00
abstract::Programmed cell death (PCD) is involved in the physiological regulation of lymphocyte turnover, as well in the antigen-driven selection of T and B cells. Here it is shown that the immunomodulator linomide (quinoline-3-carboxamide) inhibits the apoptotic decay of peripheral T lymphocytes in response to three different ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240108
更新日期:1994-01-01 00:00:00
abstract::Surfactant protein A (SP-A) and surfactant protein D (SP-D) are collectins, and both proteins were shown to interact with influenza A virus and alveolar macrophages. However, it is not known whether SP-A and SP-D can serve as opsonins for the phagocytosis of influenza A virus by alveolar macrophages. In the present st...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270413
更新日期:1997-04-01 00:00:00
abstract::The involvement of Lyt-2+ T cells in the immunological control of cutaneous leishmaniasis was assessed by studying the course of the disease in mice in which the number of these cells was reduced by treatment with anti-Lyt-2 monoclonal antibody (mAb). Administration of anti-Lyt-2 mAb exacerbated Leishmania major-induc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171007
更新日期:1987-10-01 00:00:00
abstract::Hypoxia and glucose deprivation are often observed in the microenvironment surrounding solid tumors in vivo. However, how they interfere with MHC class I antigen processing and CD8(+) T-cell responses remains unclear. In this study, we analyzed the production of antigenic peptides presented by classical MHC class I in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545835
更新日期:2016-04-01 00:00:00
abstract::CD22 is a B lymphocyte-specific membrane protein that functions as an adhesion molecule via its interactions with a subset of alpha 2-6-linked sialic acid-containing glycoproteins. Engagement of CD22 with a monoclonal antibody (HB22.23) that blocks the binding of CD22 to its ligands results in rapid CD22 tyrosine phos...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260610
更新日期:1996-06-01 00:00:00
abstract::cDNA of the T cell receptor beta (TCRB) have been isolated from the anuran amphibian Xenopus and they show strong structural homology to TCRB sequences of other vertebrates. Ten BV families, two D segments, ten J segments, and a single C region have been defined so far. Each V family consists of one to two members per...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270327
更新日期:1997-03-01 00:00:00
abstract::The nature of Ig receptors carried by lipopolysaccharide (LPS)-stimulated mouse spleen B cells was analyzed by surface iodination, direct antiserum precipitation and polyacrylamide gel electrophoresis. LPS activation led to a rapid decrease of surface IgD to 20 % and 80 % of the original level on days three and five o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830070307
更新日期:1977-03-01 00:00:00
abstract::The heterogeneous nuclear ribonucleoprotein A2 (hnRNP-A2) has been described as an important autoantigen in rheumatoid arthritis (RA) since it is targeted by autoantibodies, autoreactive T cells, and is aberrantly expressed in synovial cells in patients. To identify hnRNP-A2-specific T-cell epitopes possibly associate...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939482
更新日期:2010-06-01 00:00:00
abstract::HSP gp96-based vaccines have been trialled in rodent models and, more recently, in humans. Better understanding of gp96's immunomodulatory role will help with the design of more effective strategies for treatment of cancer and infectious diseases. In this study, we monitored the activities of T cells and activation of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939593
更新日期:2009-11-01 00:00:00
abstract::We examined T cell development and T cell repertoire in transgenic mice expressing a single T cell receptor (TCR) alpha chain derived from the H-2Db-lymphocytic choriomeningitis virus (LCMV)-specific cytolytic T lymphocyte (CTL) clone P14. To generate these alpha P14 mice, mice transgenic for the P14 TCR alpha chain w...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250937
更新日期:1995-09-01 00:00:00
abstract::The role of TGF-beta1 in the regulation of T cell responses has been perplexing, possibly because it is dependent on the type of T cell being regulated and its cytokine microenvironment. In the present study, we demonstrate that TGF-beta1 has a profound inhibitory effect on naive CD4+ T cell undergoing differentiation...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<2101::AID-IMMU2101>3
更新日期:2000-07-01 00:00:00
abstract::We have investigated the vaccine potential of two peptides derived from the 6-kDa early secretory antigenic target (ESAT)-6 antigen in the mouse model of tuberculosis. The peptides were both strongly immunogenic in B6CBAF1 (H-2b,k) mice and primed recall responses of the same intensity after immunization. However, bot...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200006)30:6<1724::AID-IMMU1724>3
更新日期:2000-06-01 00:00:00
abstract::Caspases are essential mediators of cytokine release and apoptosis. Additionally, caspase activity is required for the proliferation of naive T lymphocytes. It remained unclear how proliferating cells are able to cope with the pro-apoptotic activity especially of effector caspases-3 and -7. Possible reasons might incl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838211
更新日期:2008-07-01 00:00:00
abstract::Spleen cells from trypanosome-infected mice strongly suppressed lymphocyte stimulation induced in normal spleen cell populations by lipopolysaccharide, concanavalin A or allogeneic (H-2-different) stimulator cells. This suppression was not H-2-restricted, as responses of spleen lymphocytes both allogeneic and syngenei...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090306
更新日期:1979-03-01 00:00:00
abstract::NKR-P1 is a type II transmembrane protein which acts as an activation receptor on natural killer (NK) cells. The cytoplasmic domains of the CD4, CD8 and 4-1BB receptors contain the sequence Cys-X-Cys-Pro which is directly involved in coupling to another pair of cysteines in the N-terminal domain of the src family tyro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270111
更新日期:1997-01-01 00:00:00
abstract::BALB/c mice were repeatedly immunized with microgram doses of benzylpenicilloylated Ascaris protein(s) (BPO9Asc) in alum. At different stages of the immune response, BPO21 eicosa-L-lysine or two analogs containing one or two hydrophobic p-oxymethylbenzyl-3 beta-cholestanyl succinate (OSuco) groups were injected. When ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140113
更新日期:1984-01-01 00:00:00
abstract::Induction of cytolytic activity of peripheral blood mononuclear cells towards target cells was studied by preparing bispecific F(ab')2 which was composed of two Fab' fragments, one of which was derived from anti-CD3 monoclonal antibody and the other from anti-tumor monoclonal antibody. After reduction of the interchan...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190814
更新日期:1989-08-01 00:00:00
abstract::The T cell receptor is a molecular complex compriSed of a clonally-restricted heterodimer (Ti) responsible for specific antigen recognition and a set of invariant CD3 peptides termed gamma, delta, epsilon, zeta and eta. The latter are believed to be involved in transmembrane signaling events given that monoclonal anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190525
更新日期:1989-05-01 00:00:00
abstract::Hybrid virus-like particles (VLP) were prepared by self-assembly of the modified porcine parvovirus (PPV) VP2 capsid protein carrying a CD8+ or CD4+ T cell epitope. Immunization of mice with a single dose of these hybrid pseudo-particles, without adjuvant, induced strong cytotoxic T lymphocyte and T helper (Th) respon...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199804)28:04<1401::AID-IMM
更新日期:1998-04-01 00:00:00
abstract::The in vitro immune response of murine spleen cells to the T cell-independent antigen, 2,4-dinitrophenyl-beta-alanyl-glycyl-glycyl-N(2-aminoethyl)carbamyl-methylated Ficoll (DAGG-Ficoll), requires the presence of macrophages, or 24-hr culture supernates from peritoneal cells. Complete reconstitution of the response by...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081105
更新日期:1978-11-01 00:00:00
abstract::Previously we implicated c-Jun N-terminal kinase (JNK) as an element that is involved in signal integration during co-stimulation of T lymphocytes. This pathway has now been traced to an upper level, comprising MAPKK SEK1/MKK4/JNKK1 which, similarly to JNK, must receive input both from the TCR and CD28. A large portio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199808)28:08<2320::AID-IMM
更新日期:1998-08-01 00:00:00
abstract::To elucidate the mechanism of autoimmune disease in neonatally thymectomized (NTX) mice, we have investigated the responsiveness of the self-reactive T cells which have not undergone clonal deletion in such animals. Consistent with a recent report (Yuuki et al., Eur. J. Immunol. 1990. 20: 1475), T cells bearing V beta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210310
更新日期:1991-03-01 00:00:00
abstract::Regulatory T (Treg) cells are a constitutively immunosuppressive subtype of T cells that contribute to the maintenance of immunological self-tolerance and immune homeostasis. However, the molecular mechanisms involved in the regulation of Treg cells remain unclear. In the present study, we identified ubiquitously expr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343394
更新日期:2014-02-01 00:00:00
abstract::IL-7 plays many fundamental roles during murine B lineage development. One reported function is to maintain progenitors in a developmentally immature state by preventing differentiation to the surface IgM (sIgM)(+) stage. Withdrawal of IL-7 from cultures has been shown to lead to increases in mature traits such as RAG...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425400
更新日期:2004-10-01 00:00:00
abstract::Major histocompatibility complex (MHC) class II-associated antigen presentation is mainly linked to processing of exogenous antigens upon cellular uptake by endocytosis, but has also been observed for endogenously synthesized antigens. We have studied the MHC class II-associated presentation of the endogenously synthe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251230
更新日期:1995-12-01 00:00:00
abstract::The biochemical behavior and peptide binding properties of a soluble form of the human class II DR4Dw4 molecule (PI-DR4Dw4) were compared to DR4Dw4 molecules containing the transmembrane and cytoplasmic domains that were purified both from B and transfected chinese hamster ovary cells. Recombinant and B cell-derived D...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230112
更新日期:1993-01-01 00:00:00
abstract::In previous publications it was shown by avidity measurements, cross-reactivity patterns and genetic analyses, that the tetrapeptide T-T-G-G is the immuno-dominant epitope of the synthetic polypeptide (T, G)-A--L. In the present study this close immunological relationship between the random multichain copolymer (T, G)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060905
更新日期:1976-09-01 00:00:00