Abstract:
:Hybrid virus-like particles (VLP) were prepared by self-assembly of the modified porcine parvovirus (PPV) VP2 capsid protein carrying a CD8+ or CD4+ T cell epitope. Immunization of mice with a single dose of these hybrid pseudo-particles, without adjuvant, induced strong cytotoxic T lymphocyte and T helper (Th) responses against the reporter epitope. The Th response was characterized by a Th1 phenotype. We also analyzed in vitro the uptake mechanism of these parvovirus-like particles and the processing requirements associated with presentation by MHC molecules. Although previously shown to be presented by MHC class I molecules, these particles also enter very efficiently the MHC class II endocytic pathway, and behave as conventional exogenous antigens. Indeed, the processing of chimeric PPV:VLP was performed in endosomal/lysosomal acidic vesicles and the presentation of the foreign epitope carried by these particles was sensitive to brefeldin A and cycloheximide, showing that the foreign peptide was loaded on nascent MHC class II molecules. These results give some indication of how PPV:VLP can be presented by MHC class I and class II molecules, and underscore the wide potency of such VLP system to deliver foreign antigens for vaccine design.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Lo-Man R,Rueda P,Sedlik C,Deriaud E,Casal I,Leclerc Cdoi
10.1002/(SICI)1521-4141(199804)28:04<1401::AID-IMMsubject
Has Abstractpub_date
1998-04-01 00:00:00pages
1401-7issue
4eissn
0014-2980issn
1521-4141pii
10.1002/(SICI)1521-4141(199804)28:04<1401::AID-IMMjournal_volume
28pub_type
杂志文章abstract::Fc gamma receptor-bearing U937 cells, when incubated in serum-free buffer, were found to release spontaneously a suppressor material for pokeweed mitogen-drigen IgG synthesis which could be retained on Sepharose 4B-IgG immunosorbents. Immunosorbents coupled with IgM or F (ab')2 fragments of IgG were unable to retain t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160106
更新日期:1986-01-01 00:00:00
abstract::BALB/c mice were immunized intranasally with either soluble ovalbumin (OVA) or OVA entrapped in liposomes. The effect of adding Sigma cholera toxin B subunit (sCT-B), which contained low amounts of cholera holotoxin (CT), or recombinant CT-B (rCT-B) which was free from CT, as mucosal adjuvants was also investigated. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250417
更新日期:1995-04-01 00:00:00
abstract::The regulation of neutrophil half-life by members of the coagulation cascade is critical for the resolution of the inflammatory response. We have demonstrated that soluble fibrinogen (sFbg) delays human neutrophil (PMN) apoptosis through a mechanism that involves CD11b interactions, and phosphorylation of focal adhesi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323512
更新日期:2003-05-01 00:00:00
abstract::Several drugs targeting members of the TNF superfamily or TNF receptor superfamily (TNFRSF) are widely used in medicine or are currently being tested in therapeutic trials. However, their mechanism of action remains poorly understood. Here, we explored the effects of TNFRSF co-stimulation on murine Foxp3+ regulatory T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948393
更新日期:2020-07-01 00:00:00
abstract::It is widely accepted that females have superior immune responses than males, but the ways by which sex hormones may enhance T cell responses are still poorly understood. In the present study, we analyzed the effect of estrogens on CD4 T cell activation and differentiation after immunization with exogenous antigens. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/immu.200310027
更新日期:2003-02-01 00:00:00
abstract::The role of the invariant chain (Ii), an MHC class II-associated chaperone, in B cell development is controversial. Ii deficient mice (Ii(-/-) mice) show a defect in B cell development. This defect has been attributed to the absence of a fragment liberated from the Ii by intramembranous proteolysis. It was proposed th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425246
更新日期:2004-08-01 00:00:00
abstract::Thapsigargin (TG), which inhibits endoplasmic reticulum-dependent Ca(2 +)-ATPase and thereby increases cytosolic Ca(2 +), has been reported to cause apoptosis in T lymphocytes another cell types. In this study, we investigated the molecular mechanisms that are involved in the apoptosis induced by TG in T cell hybridom...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200106)31:6<1757::aid-immu1757>3
更新日期:2001-06-01 00:00:00
abstract::The immunocytochemical characteristics of large granular lymphocytes (LGL), isolated from the liver sinusoids of euthymic and athymic (nude) rats, were investigated in electron microscopy by the immunoperoxidase technique. The LGL were found positive for MRC OX-8 (natural killer cells and cytotoxic/suppressor T cells)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171006
更新日期:1987-10-01 00:00:00
abstract::Riboflavin, also known as vitamin B2 , is converted by riboflavin kinase (RFK) into flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors of dehydrogenases, reductases, and oxidases including the phagocytic NADPH oxidase 2 (Nox2). Riboflavin deficiency is common in young adul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343940
更新日期:2014-03-01 00:00:00
abstract::NK cells can kill transformed, infected and stressed cells while most normal cells are spared. NK cells are activated by various endogenous self-ligands, some of which are actually expressed by normal cells. Thus, NK cells are inherently self-reactive and consequently, potentially auto-aggressive. How these cells are ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200838755
更新日期:2008-11-01 00:00:00
abstract::NKp44 (NCR2) is a distinct member of natural cytotoxicity receptors (NCRs) family that can induce cytokine production and cytolytic activity in human NK cells. Heparan sulfate proteoglycans (HSPGs) are differentially expressed in various normal and cancerous tissues. HSPGs were reported to serve as ligands/co-ligands ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445177
更新日期:2015-04-01 00:00:00
abstract::The biochemical behavior and peptide binding properties of a soluble form of the human class II DR4Dw4 molecule (PI-DR4Dw4) were compared to DR4Dw4 molecules containing the transmembrane and cytoplasmic domains that were purified both from B and transfected chinese hamster ovary cells. Recombinant and B cell-derived D...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230112
更新日期:1993-01-01 00:00:00
abstract::Although originally described in tumor-bearing hosts, myeloid-derived suppressor cells (MDSC) have been detected under numerous pathological situations that cause enhanced demand of myeloid cells. Thus, MDSC might be part of a conserved response to different endogenous and exogenous stress signals, including inflammat...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200939892
更新日期:2009-10-01 00:00:00
abstract::The responses of a single cloned T cell line to three different class II major histocompatibility complex (MHC) ligands have been compared for avidity, determined by inhibition with anti-T cell receptor Fab fragments directed at two different receptor epitopes, and for ease of inhibition with anti-CD4 antibody. It has...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190114
更新日期:1989-01-01 00:00:00
abstract::Although alterations in CD3-associated signal-transducing molecules in tumor-infiltrating T cells of patients with advanced cancer have been previously described, the mechanism behind these changes is not known. We demonstrate that macrophages isolated from metastatic lymph nodes of patients with malignant melanoma do...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260620
更新日期:1996-06-01 00:00:00
abstract::Previous studies have shown that the fecal flora of patients with Crohn's disease (CD) differed from the flora of healthy subjects by a higher number of anaerobic gram-positive coccoid rods. Sera from patients with CD agglutinated four strains of coccoid rods (Me44, C18, Me46 and Me47) more frequently and stronger tha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150823
更新日期:1985-08-01 00:00:00
abstract::The pathogenesis of the decline of CD4 lymphocyte counts accompanying the typical course of HIV-1 infection is not completely defined and might be related to a differential susceptibility of naive and memory cells to HIV-1 exposure. Here, we examined the effects induced by heat-inactivated HIV-1 virions on these lymph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199906)29:06<1879::AID-IMM
更新日期:1999-06-01 00:00:00
abstract::Colony-stimulating factors are required for survival proliferation, differentiation and functional activation of granulocytes, macrophages and their precursor cells. In the present report, however, we demonstrate antiproliferative activity of recombinant human (rh) granulocyte-macrophage colony-stimulating factor (GM-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180308
更新日期:1988-03-01 00:00:00
abstract::The anti-PC antibodies of BALB/c origin bear predominantly the idiotype characteristic of the phosphorylcholine (PC)-binding T15 idiotype than sera from adult mice, and, unlike the latter, they also contain detectable amounts of anti-T15 antibodies. By 2 weeks of age the anti-T15 antibodies are no longer detectable wh...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060710
更新日期:1976-07-01 00:00:00
abstract::CTLA-4 is a crucial immune regulator that mediates both negative costimulation signals to T cells, and regulatory T (Treg)-cell extrinsic control of effector responses. Here we present evidence supporting a novel mechanism for this extrinsic suppression, executed by the alternatively spliced soluble CTLA-4 isoform (sC...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242529
更新日期:2013-05-01 00:00:00
abstract::Thymectomy was performed on the urodele amphibian Pleurodeles waltlii Michah. from the 5th to the 20th week of larval development. Thymectomized animals were allografted at 7 months of age. The results obtained are as follows: (1) Pleurodeles thymectomized before 10 weeks always tolerated allografts: (2) Pleurodeles t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050215
更新日期:1975-02-01 00:00:00
abstract::During mammalian pregnancy, one or more semiallogeneic fetuses gestate in direct contact with the maternal circulation and uterine tissue. However, a damaging maternal immune response is not normally provoked. We studied two possible mechanisms for this maternal-fetal tolerance, alone and in combination. First, we dir...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3479::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::The lymph node cells (LNC) activated in vivo by liver regeneration following partial hepatectomy of mice (primed lymph node cells) respond to regenerating liver cells in vitro with typical secondary immune response characteristics (Miyahara, S. et al., Eur. J. Immunol. 1983. 13: 878). These LNC activated in vivo suppr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131105
更新日期:1983-11-01 00:00:00
abstract::E-selectin is a cytokine-inducible membrane glycoprotein capable of mediating adhesion of leukocytes to endothelial cells. It is highly glycosylated, containing 11 sites for N-linked glycosylation. N-Glycosylation of E-selectin was analyzed by endoglycosidase treatment. Analysis of immunoprecipitated E-selectin from h...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250907
更新日期:1995-09-01 00:00:00
abstract::The T cell receptor (TcR) on CD8+ T lymphocytes recognizes a complex which consists of a major histocompatibility complex (MHC) heavy chain, beta 2-microglobulin (beta 2M), and peptide on the surface of antigen-presenting cells. Mutational analyses have suggested that the TcR recognizes both the alpha 1 and alpha 2 do...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231145
更新日期:1993-11-01 00:00:00
abstract::To identify environmental stimuli that induce dendritic cell (DC) maturation, we exposed human monocyte-derived immature DC to apoptotic or necrotic cells and measured the levels of expression of costimulatory molecules and cytokine production. While most necrotic or apoptotic cells did not have any effect, some induc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200002)30:2<705::AID-IMMU705>3.0
更新日期:2000-02-01 00:00:00
abstract::Upon antigen-specific or allogeneic activation, T cells sharply increase their metabolic activity to cope with augmented needs for proliferation and effector functions. Therefore, enzymes involved in energy metabolism constitute attractive targets to modulate the activity of pathogenic effector T cells in the setting ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546152
更新日期:2016-09-01 00:00:00
abstract::A reverse hemolytic plaque assay has been developed which specifically detects secretion of human immune interferon (Hu IFN-gamma) at the single cell level. Unstimulated peripheral blood lymphocytes from healthy adult volunteers spontaneously secreted IFN-gamma. Stimulation of these cells with concanavalin A, phytohem...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130308
更新日期:1983-03-01 00:00:00
abstract::Role of CD45 in B cell antigen receptor (BcR)-mediated signaling events in mature B cells was examined using BAL-17 and its CD45-negative clones. In the CD45-negative clones, BcR stimulation induced tyrosine phosphorylation almost identical to the parental cells, with a few exceptions of reduced phosphorylation, espec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250823
更新日期:1995-08-01 00:00:00
abstract::The physical association of CD5 and the T cell antigen receptor (TcR)/CD3 complex on the surface of intact human lymphocytes was investigated using co-capping experiments and fluorescence resonance energy transfer (FRET) analyses. Antibody-induced capping of CD5 or CD3 and double indirect immunofluorescence labeling r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230530
更新日期:1993-05-01 00:00:00