Abstract:
:The physical association of CD5 and the T cell antigen receptor (TcR)/CD3 complex on the surface of intact human lymphocytes was investigated using co-capping experiments and fluorescence resonance energy transfer (FRET) analyses. Antibody-induced capping of CD5 or CD3 and double indirect immunofluorescence labeling revealed a specific co-localization of a significant fraction of CD3 and CD5 molecules on the T cell surface. By means of FRET measurements we studied further the physical proximity of CD5 and the TcR/CD3 complex at the surface of normal lymphocytes. Significant fluorescence energy transfer was measured between CD5 and CD3 molecules indicating that the associated molecules were within 10 nm of one another. No energy transfer was observed between the integrin alpha 4 beta 7 and CD3 or CD5. The close physical proximity measured between CD5 and CD3 correlates with our co-capping data and taken together the results show that the association of CD5 and the TcR/CD3 complex first detected by immunoprecipitation occurs on the surface of human T cells under physiologically relevant conditions.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Osman N,Lazarovits AI,Crumpton MJdoi
10.1002/eji.1830230530subject
Has Abstractpub_date
1993-05-01 00:00:00pages
1173-6issue
5eissn
0014-2980issn
1521-4141journal_volume
23pub_type
杂志文章abstract::Mouse peritoneal macrophages activated with interferon-gamma (IFN-gamma) and lipopolysaccharide produce substantial amounts of nitric oxide (NO), which correlates with the elimination of the intracellular protozoan parasite Leishmania major. Both the production of NO and the leishmanicidal function of the activated ma...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220223
更新日期:1992-02-01 00:00:00
abstract::T cell stimulation of B cell proliferation during T-B collaboration requires membrane-bound stimulatory ligands, such as CD40 ligand and the secretion of soluble cytokines, such as IL-4. Nevertheless, it remains unclear whether T cell contact is required to provoke each consecutive B cell division, or whether B cells ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200104)31:4<1150::aid-immu1150>3
更新日期:2001-04-01 00:00:00
abstract::The immune system relies on homeostatic mechanisms in order to adapt to the changing requirements encountered during steady-state existence and activation by antigen. For T cells, this involves maintenance of a diverse repertoire of naïve cells, rapid elimination of effector cells after pathogen clearance, and long-te...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200939444
更新日期:2009-08-01 00:00:00
abstract::Lipid antigens are presented to T cells by the non-polymorphic MHC class I-related CD1 molecules. Microsomal triglyceride transfer protein (MTP) is an endoplasmic reticulum (ER)-resident chaperone that has been shown to lipidate the group 2 CD1 molecule CD1d and thus to regulate its function. We now report that MTP al...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200738102
更新日期:2008-08-01 00:00:00
abstract::Since the discovery of antibody-producing B cells in chickens six decades ago, chickens have been a model for B-cell development in gut-associated lymphoid tissue species. Here we describe targeting of the immunoglobulin light chain locus by homologous recombination in chicken primordial germ cells (PGCs) and generati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546171
更新日期:2016-09-01 00:00:00
abstract::The most common reason for heart failure in young adults is dilated cardiomyopathy often resulting from myocarditis. Clinical studies and animal models provide evidence that an autoimmune response against heart myosin is the underlying reason for the disease. IL-12 has been suggested to play a key role in development ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636484
更新日期:2006-11-01 00:00:00
abstract::The specificity and efficiency of endogenous peptides in the HLA class I binding have been investigated by the use of a simple procedure that is based on the serological detection of the folding of HLA class I alpha chains that is induced by the binding to specific peptides in the presence of beta 2 microglobulin. HLA...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220834
更新日期:1992-08-01 00:00:00
abstract::There is a current need for safe, cheap, and effective vaccine adjuvants, to combine with sub-unit antigens to enhance their immunogenicity. In this study we have used probiotic Bacillus subtilis spores, known to be safe and fully tolerated by ingestion in man, and explored their ability to influence the magnitude and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636875
更新日期:2007-06-01 00:00:00
abstract::Antigen-specific immunotherapy is an attractive strategy for cancer control. In the context of antiviral vaccines, adenoviral vectors have emerged as a favorable means for immunization. Therefore, we chose a strategy combining use of these vectors with another successful approach, namely linkage of the vaccine antigen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939543
更新日期:2009-10-01 00:00:00
abstract::Interferon-gamma inducing factor (IGIF) is a recently identified cytokine which stimulates the production of interferon-gamma (IFN-gamma) by T cells and enhances natural killer (NK) cell cytolytic activity. Protein fold recognition, structure prediction and comparative modeling have revealed that IGIF is a member of t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271107
更新日期:1997-11-01 00:00:00
abstract::Immunomodulatory treatment paradigms have been applied to animal models of T cell-mediated autoimmune diseases in an attempt to develop an immunospecific and non-toxic form of therapy which can be applied to humans. These treatment paradigms are often directed to T cells with a restricted T cell receptor repertoire or...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240926
更新日期:1994-09-01 00:00:00
abstract::Bone marrow-derived macrophages were stained with a variety of monoclonal antibodies (mAb) with specificity for the mycobacterial heat shock protein (hsp) 60 or with specific rabbit antiserum raised against mycobacterial hsp 60. The mAb ML30 as well as the specific antiserum brightly stained bone marrow-derived macrop...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210437
更新日期:1991-04-01 00:00:00
abstract::Mucosa-associated lymphoid tissue 1 (Malt1) regulates immune cell function by mediating the activation of nuclear factor κB (NF-κB) signaling through both its adaptor and proteolytic function. Malt1 is also a target of its own protease activity and this self-cleavage further contributes to NF-κB activity. Until now, t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847597
更新日期:2018-10-01 00:00:00
abstract::Dendritic cells (DC) are unique in their ability to present antigen to naive T cells, and therefore play a central role in the initiation of immune responses. Characterization of DC-specific genes may help to unravel the mechanism underlying their potent antigen presenting capacity. Here we describe the identification...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3585::AID-IMMU3585>
更新日期:2000-12-01 00:00:00
abstract::We recently constructed IL-15 transgenic (Tg) mice using cDNA encoding a secretable isoform of the IL-15 precursor protein under the control of an MHC class I promoter. The IL-15 Tg mice exhibited resistance against a primary infection with Listeria monocytogenes. The numbers of memory CD8(+) T cells were markedly inc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200103)31:3<757::aid-immu757>3.0
更新日期:2001-03-01 00:00:00
abstract::Mice carrying the lpr gene, SCG and MRL-lpr/lpr mice, were used to characterize the phenotype and lpr gene of abnormally proliferating T cells in these mice. A major population which expanded in these mice were T cells expressing intermediate (int) levels of T cell receptor (TCR) (and CD3) and the phenotype of interle...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260702
更新日期:1996-07-01 00:00:00
abstract::Cytomegaloviruses (CMVs) are highly prevalent, persistent human pathogens that not only evade but also shape our immune responses. Natural killer (NK) cells play an important role in the control of CMV and CMVs have in turn developed a plethora of immunoevasion mechanisms targeting NK cells. This complex interplay can...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201646762
更新日期:2018-01-01 00:00:00
abstract::A human lymphoid cell ine (S95) with surface-located IgG is described. The line was derived from a patient with Hodgkin's disease and is aneuploid. Its characteristics are suggestive of a malignant B lymphocyte derivation, but no ultimate proof of malignancy can be forwarded. The line produces immunoglobulin (IgG) whi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050803
更新日期:1975-08-01 00:00:00
abstract::IFN-gamma plays a crucial role in the defense against infection with Candida albicans. Since IL-18 and IL-12 are strong stimuli of IFN-gamma production, we investigated whether endogenous IL-18 and IL-12 are involved in the host defense during disseminated candidiasis. IL-18 knockout (IL-18-/-) mice, but not IL-12-/- ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323737
更新日期:2003-12-01 00:00:00
abstract::CD30 is a costimulatory molecule of the TNF receptor superfamily, expressed on activated T and B cells. Previously, we have shown in a murine asthma model the crucial role of CD30 signaling for the development of this Th2-cell-mediated disease. In the present study, we investigated the role of CD30 in the maintenance ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839004
更新日期:2009-07-01 00:00:00
abstract::Only activated and effector memory T cells are thought to access non-lymphoid tissues. In contrast, naive T cells are thought to circulate only between the blood, lymph and secondary lymphoid organs. We examined the phenotype of endogenous T cells in various non-lymphoid organs and showed that a subset of cells exhibi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535539
更新日期:2006-06-01 00:00:00
abstract::Mice with homologous disruption of the gene coding for either the p35 subunit or the p40 subunit of interleukin-12 (IL-12) and derived from a strain genetically resistant to infection with Leishmania major have been used to study further the role of this cytokine in resistance to infection and the differentiation of f...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260722
更新日期:1996-07-01 00:00:00
abstract::This study was performed to investigate whether gamma delta T cells could also be divided into subsets, identified by a cytokine profile, as described for alpha beta T helper (Th) cell subsets. Cytokine production was studied in 22 gamma delta T cell clones obtained from the synovial fluid and peripheral blood of one ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240923
更新日期:1994-09-01 00:00:00
abstract::The death of individual cells is a frequent and physiological event in the mammalian immune system and most often occurs by apoptosis. It is becoming increasingly clear that cell death is also induced during bacterial infections. Here we report that, in addition to the apoptotic form already established, a necrosis-li...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324582
更新日期:2004-05-01 00:00:00
abstract::The recent discovery of IL-17-producing CD4(+) Th subset significantly revised the Th1/Th2 dichotomy model proposed by Mosmann and Coffman almost two decades ago. Th17 cells are involved in the pathogenesis of many human autoimmune diseases. Th17 cells, their developmental pathways and their effector functions, theref...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839105
更新日期:2009-03-01 00:00:00
abstract::At the surface of the murine T lymphoma cell line RMA-S, the expression of "empty" class I molecules can be dramatically enhanced by culture at 26 degrees C. These class I molecules are unstable following transfer to 37 degrees C unless they are loaded with exogenously added peptides. Class I heterodimers that have fa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220639
更新日期:1992-06-01 00:00:00
abstract::Granzyme A (TSP-1) is a serine esterase expressed in cytotoxic T and natural killer (NK) cell lines. Its presence in in vivo primed T cells is disputed at present. Here we show that normal rat NK cells contain granzyme A in vivo and that its expression is augmented by their short-term in vitro treatment with IL 2. Gra...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190328
更新日期:1989-03-01 00:00:00
abstract::Murine splenic T lymphocytes display maximal cellular myc gene (c-myc) expression already 3 h after concanavalin A stimulation and subsequent down-regulation before the onset of DNA synthesis. Stimulation by leucoagglutinin in the presence or absence of interleukin 2 leads to only low initial levels of c-myc-specific ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160319
更新日期:1986-03-01 00:00:00
abstract::Mice deficient for the adapter protein SLP65 (BLNK) show a partial block in early B cell development, reduced numbers of mature B cells in the periphery, an absence of B1 cells and a reduction of IgM and IgG3 serum immunoglobulin levels. A strikingly similar phenotype is observed in Btk-deficient mice. To investigate ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<2164::aid-immu2164>3
更新日期:2001-07-01 00:00:00
abstract::Autoimmune diseases including multiple sclerosis (MS) are the result of an imbalanced immune tolerance network. Dendritic cells (DCs) are key players in both initiating immunity (immunogenic DCs) and regulating immune responses (tolerogenic DCs = tolDCs) and are potential targets for the treatment of MS. While the imm...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201242402
更新日期:2012-03-01 00:00:00