Abstract:
:We recently constructed IL-15 transgenic (Tg) mice using cDNA encoding a secretable isoform of the IL-15 precursor protein under the control of an MHC class I promoter. The IL-15 Tg mice exhibited resistance against a primary infection with Listeria monocytogenes. The numbers of memory CD8(+) T cells were markedly increased in the IL-15 Tg mice following Listeria infection accompanied by sustained IL-15 production. The increased CD44(+)CD8(+) T cells in the infected IL-15 Tg mice were not specialized to recognize Listeria-specific antigen but produced a large amount of IFN-gamma in response to bystander stimulation exogenous IL-15 in combination with IL-12. Furthermore, Listeria-specific Th1 response by CD4(+) T cells was significantly augmented in the IL-15 Tg mice compared with control mice following Listeria infection. In vivo depletion of the CD8(+) T cells by anti-CD8 monoclonal antibody and adoptive transfer of the T cells from naive IL-15 Tg mice indicated that the CD8(+) T cells functioned not only to eliminate bacteria at the early stage of infection but also to promote Th1 response to L. monocytogenes. Overexpression of IL-15 shed light on a novel role of memory CD8(+) T cells in early protection and promotion of Th1 response against a primary infection with L. monocytogenes.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Yajima T,Nishimura H,Ishimitsu R,Yamamura K,Watase T,Busch DH,Pamer EG,Kuwano H,Yoshikai Ydoi
10.1002/1521-4141(200103)31:3<757::aid-immu757>3.0keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
757-66issue
3eissn
0014-2980issn
1521-4141pii
10.1002/1521-4141(200103)31:3<757::AID-IMMU757>3.0journal_volume
31pub_type
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journal_title:European journal of immunology
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