Abstract:
:Defined tumor-associated antigens (TAA) are attractive targets for anti-tumor immunotherapy. Here, we describe a novel genome-wide approach to identify multiple TAA from any given tumor. A panel of transplantable thymomas was established from an inbred p53-/- mouse strain. The resulting tumors were examined for gene expression by mRNA microarray scanning. This analysis revealed heterogeneity of the tumors in agreement with the assumption that they represent different tumorigenic events. Several genes were overexpressed in one or more of the tumors. To examine whether overexpressed genes might be used to identify TAA, mice were immunized with mixtures of peptides representing putative cytotoxic T cell epitopes derived from one of the gene products. Indeed, such immunized mice were partially protected against subsequent tumor challenge. Despite being immunized with bona fide self antigens, no clinical signs of autoimmune reactions were observed. Thus, it appears possible to evaluate the entire metabolism of any given tumor and use this information rationally to identify multiple epitopes of value in the generation of tumor-specific immunotherapy. We expect that human tumors express similar tumor-specific metabolic imprints, which may be used to identify patient-specific arrays of TAA. This may enable a multi-epitope based immunotherapy with improved prospects of clinical tumor rejection.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Mathiassen S,Lauemøller SL,Ruhwald M,Claesson MH,Buus Sdoi
10.1002/1521-4141(200104)31:4<1239::aid-immu1239>3keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
1239-46issue
4eissn
0014-2980issn
1521-4141pii
10.1002/1521-4141(200104)31:4<1239::AID-IMMU1239>3journal_volume
31pub_type
杂志文章abstract::Incomplete allelic exclusion of TCRa gene rearrangement permits the generation of dual Valpha T cells, though the issues of their frequency and whether both alphabeta pairs participate in thymic selection have not been resolved. Both questions have been investigated using lymphocytes from mice hemizygous at the TCRa l...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199807)28:07<2115::AID-IMM
更新日期:1998-07-01 00:00:00
abstract::We have investigated the vaccine potential of two peptides derived from the 6-kDa early secretory antigenic target (ESAT)-6 antigen in the mouse model of tuberculosis. The peptides were both strongly immunogenic in B6CBAF1 (H-2b,k) mice and primed recall responses of the same intensity after immunization. However, bot...
journal_title:European journal of immunology
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doi:10.1002/1521-4141(200006)30:6<1724::AID-IMMU1724>3
更新日期:2000-06-01 00:00:00
abstract::The problem of the structural relationship between suppressive IgG-binding factor and low-affinity receptors for the Fc portion of IgG (Fc gamma RII) has not yet been solved. In the present work we have isolated a recombinant soluble Fc gamma RII containing only the two external domains of Fc gamma RII, and analyzed i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191213
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abstract::Previous studies suggested that trinitrophenyl (TNP)-modified syngeneic red cells induced humoral autoimmune response in mice with defective T cell function but not in normal mice. The ability of modified self antigen to induce autoimmune response in immunodeficient mice was further explored using the delayed-type hyp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121112
更新日期:1982-11-01 00:00:00
abstract::Reciprocal expression of CD45RA and CD45RO in human CD4+ T cells defines populations understood to be naive cells (CD45RA+CD45RO-) and memory cells (CD45RA-CD45RO+). We investigate two subsets of CD45RA-CD45RO+ CD4+ human T cells which differ by fourfold in their expression of the CD45RB isoform; one is CD45RBbright a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240536
更新日期:1994-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
abstract::A novel surface molecule, Tp90, is described which appears to be involved in an antigen-independent pathway of human T lymphocyte activation. The Tp90 molecule was identified by a monoclonal antibody (mAb), MX20, obtained from a fusion using spleen cells of a mouse immunized with cells from two T cell leukemia lines, ...
journal_title:European journal of immunology
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doi:10.1002/eji.1830170616
更新日期:1987-06-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.1830210438
更新日期:1991-04-01 00:00:00
abstract::T cell activation and cytokine secretion are important mediators of inflammation in allergic asthma. The costimulatory pathway CD28/CD80/CD86 has been shown to play an important role in T cell activation in allergic asthma, but less is known about the effect of other costimulatory molecules in allergy. The costimulato...
journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 评论,杂志文章
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更新日期:2009-06-01 00:00:00
abstract::We generated cell hybrids between mouse T cell lymphoma EL4 cells and mouse fibroblast B82 cells (BELIII and BELIV) to examine the expression of T cell receptor (TcR)/CD3 genes and their lymphoid-specific transcription factor genes, which are normally detected in EL4 cells. In BELIII and BELIV, expression of the TcR a...
journal_title:European journal of immunology
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doi:10.1002/eji.1830250947
更新日期:1995-09-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2020-02-01 00:00:00
abstract::Several groups have described that a low percentage of in vitro cultured T cell receptor (TcR) gamma/delta cells express CD8. Contrary to TcR alpha/beta cells, however, CD8 on these TcR gamma/delta cells was shown to be a CD8 alpha homodimer. We describe here that addition of interleukin-7 (IL-7) to a short-term in vi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220902
更新日期:1992-09-01 00:00:00
abstract::During the course of many human autoimmune diseases, antibodies which recognize negatively charged epitopes on self antigens are detected. Trypanosoma cruzi, an intracellular protozoan parasite capable of infecting a wide variety of vertebrates, is the cause of Chagas disease in humans. Infection with the parasite fre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221204
更新日期:1992-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636887
更新日期:2007-08-01 00:00:00
abstract::It has been reported that, following the initial expression of the T cell receptor (TcR) alpha/beta, newly generated thymocytes pass through a developmental window characterized by ineffective coupling between the alpha/beta and CD3 components resulting in resistance to deletion (negative selection). However, we now p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201125
更新日期:1990-11-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1992-06-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240108
更新日期:1994-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830070807
更新日期:1977-08-01 00:00:00
abstract::Caspases are essential mediators of cytokine release and apoptosis. Additionally, caspase activity is required for the proliferation of naive T lymphocytes. It remained unclear how proliferating cells are able to cope with the pro-apoptotic activity especially of effector caspases-3 and -7. Possible reasons might incl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838211
更新日期:2008-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838985
更新日期:2009-08-01 00:00:00
abstract::Deficiencies in terminal complement components, including the component C7, are uncommon and associated with an increased risk of recurrent systemic neisserial infection. A total of 22 molecular defects have been reported in the C7 gene with both complete (C7Q0) and subtotal (C7SD) C7 deficiencies. In this study we re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636812
更新日期:2007-05-01 00:00:00
abstract::A mouse lymphocyte surface molecule which is selectively expressed by mucosal T cells and detected with the monoclonal antibody (mAb) M290 has provisionally been identified as a beta 7 integrin. Identification was based on close homology of its beta subunit at the N terminus with the recently reported, highly distinct...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211041
更新日期:1991-10-01 00:00:00
abstract::In the present study, interleukin 1 (IL 1)-containing media from different sources, namely a murine macrophage cell line (P388D1), rabbit peritoneal macrophages, and human peripheral blood mononuclear cells, were compared for their effect on thymocyte proliferation and on collagenase and PGE2 secretion by chondrocytes...
journal_title:European journal of immunology
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更新日期:1984-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200209)32:9<2460::AID-IMMU2460>3
更新日期:2002-09-01 00:00:00
abstract::The immunosuppressor sanglifehrin A (SfA) is a member of a family of immunophilin cyclophilin A-binding molecules and does not inhibit calcineurin activity. Sanglifehrin A inhibits M-CSF-dependent macrophage proliferation by arresting the G1 phase of the cell cycle but does not affect cell viability. This immunosuppre...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
abstract::RNA from 170 different Ig-secreting hybridomas was hybridized with VH gene probes 7183, QUPC52, S107, J558, J606, 36-60, V31, X-24 and V-GAM 3.8, with the aim of comparing xid to non-xid mice, neonatal NZB to adult NZB, and the peritoneal cavity with the splenic compartments for VH gene family expression. Our results ...
journal_title:European journal of immunology
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更新日期:1988-12-01 00:00:00