Abstract:
:The problem of the structural relationship between suppressive IgG-binding factor and low-affinity receptors for the Fc portion of IgG (Fc gamma RII) has not yet been solved. In the present work we have isolated a recombinant soluble Fc gamma RII containing only the two external domains of Fc gamma RII, and analyzed its biochemical characteristics and biological activity. A cDNA encoding Fc gamma RII was mutated by the creation of a stop codon at the Lys175 codon. L cells have been transfected with this cDNA inserted into an expression vector. A cell line was obtained that secretes recombinant soluble Fc gamma RII which reacts with a monoclonal anti-Fc gamma RII antibody and binds to IgG1, IgG2a and IgG2b murine isotypes but not to IgG3 or F(ab')2 fragments of IgG2a. The secreted molecule contains two molecular species of relative molecular mass (Mr) 44,000 and 34,000-38,000 and of pI 4.5 and 6.3. They correspond to different glycosylations of a single polypeptide of Mr 19,000. After purification to homogeneity, soluble Fc gamma RII has found to suppress secondary and primary in vitro antibody responses in a dose-dependent way. The present work shows that recombinant soluble Fc gamma RII has biochemical characteristics, immunoreactivity and biological activity similar to those of suppressive IgG-binding factor.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Varin N,Sautès C,Galinha A,Even J,Hogarth PM,Fridman WHdoi
10.1002/eji.1830191213subject
Has Abstractpub_date
1989-12-01 00:00:00pages
2263-8issue
12eissn
0014-2980issn
1521-4141journal_volume
19pub_type
杂志文章abstract::An anti-T cell receptor (TcR) monoclonal antibody (mAb), LC4, directed against a human leukemic T cell line, SUP-T13, caused DNA fragmentation ("apoptosis") and cell death upon binding to this cell line. Cross-linking of receptor molecules was necessary for this effect since F(ab')2, but not Fab', fragments of LC4 cou...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191023
更新日期:1989-10-01 00:00:00
abstract::To elucidate the rapid destruction of allogeneic bone marrow (BM) grafts in nonsensitized rats we have assessed: (a) the 21-h tissue localization of 51Cr-labeled allogeneic vs. syngeneic BM cells, (b) the ability of allogeneic BM cells to proliferate in the BM or irradiated recipients, (c) the survival of allogeneic v...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140906
更新日期:1984-09-01 00:00:00
abstract::This report describes the study of the expression of an idiotype in the human population which is associated with antibodies to N-acetyl-D-glucosamine (GlcNAc) present in most human sera presumably due to streptococcal infections. The idiotype is identified with antisera and monoclonal antibodies prepared against the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130402
更新日期:1983-04-01 00:00:00
abstract::Multi-component glycopeptide libraries and single glycopeptides were used for immunization of mice with the aim of inducing strong T helper cell responses to the repetitive sequence of MUC1 expressed by human tumor cells. The glycopeptides and glycopeptide libraries were modeled upon the native human MUC1 amino acid v...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323698
更新日期:2003-06-01 00:00:00
abstract::Many pathogens are initially encountered in the gut, where the decision is made to mount an immune response or induce tolerance. The mesentric lymph node (mLN) has been shown to be involved in immune response and much more in oral tolerance induction. Furthermore, using an in vivo transplantation model, we showed rece...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040681
更新日期:2011-03-01 00:00:00
abstract::A panel of monoclonal antibodies (mAb) specific for the C gamma 2, C gamma 3 or inter C gamma 2/C gamma 3 domain epitopes was tested for inhibition of antibody-dependent cellular cytotoxicity (ADCC) specific for anti-D IgG-coated erythrocytes. Significant inhibition of ADCC was demonstrable for some antibodies having ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151015
更新日期:1985-10-01 00:00:00
abstract::Th17 cells are defined by their capacity to produce IL-17, and are important mediators of inflammation and autoimmunity. Human Th17 cells express high levels of the retinoic acid-related orphan receptor variant 2 (RORC2), but it is currently unclear whether expression of this transcription factor alone is sufficient t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838908
更新日期:2009-06-01 00:00:00
abstract::NK cells can kill transformed, infected and stressed cells while most normal cells are spared. NK cells are activated by various endogenous self-ligands, some of which are actually expressed by normal cells. Thus, NK cells are inherently self-reactive and consequently, potentially auto-aggressive. How these cells are ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200838755
更新日期:2008-11-01 00:00:00
abstract::The expression of interferon (IFN) β is highly induced in immune and nonimmune cells in response to virus infection. This upregulation is mediated at both transcriptional and posttranscriptional levels. Whereas the signaling pathways triggered by innate virus receptors leading to transcription factor activation have b...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201545616
更新日期:2015-05-01 00:00:00
abstract::A monoclonal antibody (mAb) directed to a human leukocyte 55-kDa cell surface molecule with identical cellular distribution and biochemical properties to the CD4 was able to inhibit T cell proliferation induced either in a mixed lymphocyte culture or by activation with mAb anti-CD3, anti-CD2 or phytohemagglutinin. The...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170205
更新日期:1987-02-01 00:00:00
abstract::Respiratory syncytial virus (RSV) causes severe bronchiolitis in infants worldwide. The immunological factors responsible for RSV susceptibility in infants are poorly understood. Here, we used the BALB/c mouse model of neonatal RSV infection to study the mechanisms leading to severe disease upon reexposure to the viru...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545929
更新日期:2016-04-01 00:00:00
abstract::AMPK (adenosine monophosphate-activated protein kinase) is phosphorylated (AMPK-P) in response to low energy through allosteric activation by Adenosine mono- or diphosphate (AMP/ADP). Folliculin (FLCN) and the FLCN-interacting proteins 1 and 2 (FNIP1, 2) modulate AMPK. FNIP1 deficiency patients have a AMPK-P gain of f...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201948504
更新日期:2020-07-01 00:00:00
abstract::Even after repeated immunizations with alpha(1-3)dextran (Dex) male (CBA/N x BALB/c)F1 mice fail to produce specific antibodies whereas nondefective female littermates express an idiotype-positive anti-Dex immune response. This failure of xid mice to express serum anti-Dex immunoglobulins is not only limited to immuni...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181111
更新日期:1988-11-01 00:00:00
abstract::As discussed in this review, human cytomegalovirus (HCMV) infection in healthy individuals is associated with a variable and persistent increase of NK cells expressing the CD94/NKG2C activating receptor. The expansion of NKG2C(+) NK cells reported in other infectious diseases is systematically associated with HCMV co-...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201243117
更新日期:2013-05-01 00:00:00
abstract::Large granular lymphocyte clones were prepared from normal human mononuclear cells. Seven clones were studied in detail. All had high cytotoxic activity against the natural killer (NK) cell target K562 and all were T11- and DR-positive but T4- and T8-negative: none released migration inhibitory factor, a characteristi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150614
更新日期:1985-06-01 00:00:00
abstract::Mycoplasma arthritidis produces in culture a polyclonal mitogen which is active for murine and human T lymphocytes in the presence of accessory cells (AC). We studied the requirements for processing and presentation by AC of Mycoplasma arthritidis supernatant (MAS) mitogen to human T cells. As inhibitors of AC process...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181239
更新日期:1988-12-01 00:00:00
abstract::It is widely believed that DC, but not macrophages, prime naïve T cells in vivo. Here, we investigated the ability of CD68-expressing cells (commonly defined as macrophages) in priming autoreactive T cells and initiating collagen-induced arthritis (CIA) in the mouse. For this purpose, a transgenic mouse was developed ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040598
更新日期:2011-02-01 00:00:00
abstract::A monoclonal antibody (mAb), 3A35, produced against mouse macrophages (M phi) was found to react against certain activated T cells. This mAb, a rat IgM, resulted from a cell fusion between a mouse plasmacytoma and rat lymphocytes immunized against mouse M phi. It bound more avidly to activated than to resident M phi. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170305
更新日期:1987-03-01 00:00:00
abstract::The covalent binding reaction of the third component of complement (C3) with rabbit IgG immune aggregates has been studied by enzymic digestion of C3b-IgG adducts. In these adducts C3b was radioactively labeled in the free thiol group generated during activation of the internal thioester of C3. Trypsin digestion of 14...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240316
更新日期:1994-03-01 00:00:00
abstract::The contribution of specific antibodies (Ab) to successful clearance of Pneumocystis carinii from host pulmonary tissues has received increasing attention. Sera collected from diseased recombinase-activating gene (RAG)-1(-/-), TCRbetaxdelta(-/-), TCRbeta(-/-) and Abeta(-/-) mutants as well as from aerogenic parasite-e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199908)29:08<2464::AID-IMM
更新日期:1999-08-01 00:00:00
abstract::Macrophage inflammatory protein-3alpha (MIP-3alpha)/CCL20 and MIP-3beta/CCL19 are members of the CC chemokine subfamily which exert their effects through specific receptors, CCR6 and CCR7, respectively. Previously, we have reported that human neutrophils have the capacity to produce a number of chemokines, including I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<1981::aid-immu19
更新日期:2001-07-01 00:00:00
abstract::Gout manifests as recurrent episodes of acute joint inflammation and pain due to the deposition of monosodium urate (MSU) crystals within the affected tissue in a process dependent on NLRP3 inflammasome activation. The synthesis, activation, and release of IL-1β are crucial for MSU-induced inflammation. The current st...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545798
更新日期:2016-01-01 00:00:00
abstract::Mature T cells may be produced in the thymus, or by expansion in the periphery. While thymus output of virgin cells ensures repertoire diversity, peripheral expansion increases the size of rare clones, and thus the efficiency of immune responses. We studied the role of both phenomena in the generation of the CD8+ T ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250802
更新日期:1995-08-01 00:00:00
abstract::GITR (glucocorticoid-induced TNFR family related gene) is a member of the TNFR superfamily (TNFRSF) that is expressed in different cell types, including T lymphocytes. Because of a high homology in its cytoplasmic region with other known costimulatory members of the TNFRSF, we investigated whether GITR played a costim...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324804
更新日期:2004-03-01 00:00:00
abstract::Treatment of human peripheral blood lymphocytes with cloned N alpha-desacetylthymosin alpha 1 induced interferon production. The kinetics is similar to that of mitogen-induced interferon induction. N alpha-desacetylthymosin alpha 1, in combination with mitogen, augments the amount of interferon produced. This interfer...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120314
更新日期:1982-03-01 00:00:00
abstract::To investigate the role of membrane lymphotoxin (LT)alpha1 / beta2 and its LTbeta receptor (LTbetaR) in the protective immune response to Mycobacterium bovis bacillus Calmette-Guérin (BCG) infection, we have used a soluble fusion molecule (LTbetaR-IgG1). LTbetaR-Ig treatment interferes with granuloma formation mainly ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<4002::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::Differential screening of a subtracted cDNA library led to the detection of two distinct but homologous mouse cDNA, called CTLA-2 alpha and CTLA-2 beta. The corresponding transcripts have a tissue distribution restricted to T lymphocytes, where they are inducible upon activation, and to mast cells. The open-frame regi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190409
更新日期:1989-04-01 00:00:00
abstract::Because of their plasticity and central role in orchestrating immunity and tolerance, DCs can respond to pregnancy-specific signals, thus promoting the appropriate immune response in order to support pregnancy. Here, we show that pregnancy-specific glycoprotein (PSG1a), the major variant of PSG released into the circu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142140
更新日期:2012-06-01 00:00:00
abstract::Activation pathways inducing the expression of the interferon (IFN)-gamma gene in a cytotoxic T lymphocyte (CTL) clone were studied for their effects on transcription and on mRNA stability. IFN-gamma was secreted by the CTL clone in response to the Ca2+ ionophore ionomycin when used in conjunction with either protein ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250405
更新日期:1995-04-01 00:00:00
abstract::In this study, we demonstrated that antibodies reactive with a common beta 1 subunit (CD29) of the VLA family of antigens could synergize with CD3 but not CD2 in inducing CD4 cell proliferation in a serum-free culture system. The extent of activation was similar to that obtained when CD4 cells were cultured with anti-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210212
更新日期:1991-02-01 00:00:00