Abstract:
:The surface expression of given HLA class I alleles protects target cells from lysis mediated by natural killer (NK) clones specific for these (or related) alleles. We could define two groups of NK clones specifically recognizing either Cw4 and related C alleles ("group 1") or Cw3 and related C alleles ("group 2"), respectively. Monoclonal antibodies (mAb) to class I molecules should interfere with the interaction between NK receptors and class I molecules, thus resulting in lysis of protected target cells. However, none of the numerous available mAb to class I molecules had this effect. Therefore, we attempted to select new mAb on the basis of their ability to induce lysis of Cw4- or Cw3-protected lymphoblastoid cell lines by "group 1" or "group 2" NK clones, respectively. From mice immunized with phytohemagglutinin (PHA)-activated lymphocytes expressing either Cw3 or Cw4 alleles, two mAb were selected, the 6A4 (IgG1) and the A6-136 (IgM), on the basis of their ability to induce lysis of protected target cell. Both mAb immunoprecipitated molecules which, in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gave two bands of 45 and 12 kDa, typical of the class I heavy chain and beta 2 microglobulin, respectively. It has been proposed (but not proven), that self major histocompatibility complex class I molecules protect normal cells from autologous NK cell lysis. Thus, we used the 6A4 and A6-136 mAb to assess this possibility directly. Cw4-specific ("group 1") and Cw3-specific ("group 2") NK clones were isolated from donors expressing the corresponding (or related) protective C alleles. None of these clones lysed autologous PHA-induced blasts, used as target cells. However, addition of the F(ab')2 of 6A4 mAb or the A6-136 mAb resulted in lysis of autologous target cells by "group 1" or "group 2" NK clones, respectively. These data provide direct evidence that the expression of class I molecules protects normal cells from lysis by autologous NK cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Ciccone E,Pende D,Vitale M,Nanni L,Di Donato C,Bottino C,Morelli L,Viale O,Amoroso A,Moretta Adoi
10.1002/eji.1830240434subject
Has Abstract,Author List Incompletepub_date
1994-04-01 00:00:00pages
1003-6issue
4eissn
0014-2980issn
1521-4141journal_volume
24pub_type
杂志文章abstract::Because of their plasticity and central role in orchestrating immunity and tolerance, DCs can respond to pregnancy-specific signals, thus promoting the appropriate immune response in order to support pregnancy. Here, we show that pregnancy-specific glycoprotein (PSG1a), the major variant of PSG released into the circu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142140
更新日期:2012-06-01 00:00:00
abstract::Dendritic cells (DC) are the main orchestrators of specific immune responses. Depending on microbial information they encounter in peripheral tissues, they promote the development of Th1, Th2 or unpolarized Th cell responses. In this study we have investigated the immunomodulatory effect of non-pathogenic intestinal G...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324815
更新日期:2004-05-01 00:00:00
abstract::NLRP3 inflammasome is a protein complex crucial to caspase-1 activation and IL-1β and IL-18 maturation. This receptor participates in innate immune responses to different pathogens, including the bacteria of genus Brucella. Our group recently demonstrated that Brucella abortus-induced IL-1β secretion involves NLRP3 in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848016
更新日期:2019-07-01 00:00:00
abstract::CD40 ligand (CD40L) and IL-4 are sufficient to induce resting murine B cells to divide and switch isotypes from IgM and IgD to IgG1 and IgE. Tracking of cell division following (5- and 6) carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling revealed that B cells expressed IgG1 after three cell divisions, an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<1040::AID-IMM
更新日期:1998-03-01 00:00:00
abstract::Changes in endothelial permeability are crucial in the pathogenesis of many diseases. Adenosine is one of the endogenous mediators controlling endothelial permeability under normal conditions, and an endothelial cell surface enzyme CD73 is a key regulator of adenosine production. Here we report that IFN-beta is a nove...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737793
更新日期:2007-12-01 00:00:00
abstract::Chronic macrophage inflammation is a hallmark of type 2 diabetes (T2D) and linked to the development of secondary diabetic complications. T2D is characterized by excess concentrations of saturated fatty acids (SFA) that activate innate immune inflammatory responses, however, mechanism(s) by which SFAs control inflamma...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048651
更新日期:2020-12-01 00:00:00
abstract::Studies of chronic viral infections have highlighted the phenotypic and functional heterogeneity of antigen-specific T cells and suggested that polyfunctional T cells that secrete multiple cytokines and are able to proliferate on encounter with antigen are more likely than single cytokine secretors to represent correl...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201040731
更新日期:2010-08-01 00:00:00
abstract::The in vitro recognition of acetylsalicilyl ovalbumin, penicilloylated bovine IgG (BPO-BGG) and the multichain copolymer poly-L-(Tyr, Glu)-poly-D L-Alapoly-L-Lys [(T, G)-A-L] by primed (2 x 13) F1 cells could be markedly and specifically blocked by alloantisera directed against "receptors" (R) or recognition structure...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830051012
更新日期:1975-10-01 00:00:00
abstract::The effect of Trypanosoma brucei infections on parasite-specific antibody responses was examined by immunization of infected mice with radio-attenuated trypanosomes of a noncross-reacting clone. Antibody titers (total Ig) against variant surface antigen were determined by indirect immunofluorescence. The suppression r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101216
更新日期:1980-12-01 00:00:00
abstract::The turnover of mature and immature B cells defined by a range of cell surface markers was investigated by feeding normal or bcl-2-transgenic (bcl-2-Tg) mice 5'-bromo-2-deoxyuridine (BrdUrd) for up to 6 weeks. In peripheral lymphoid tissue, B cells accumulated BrdUrd with a 50% labeling time of 4.3 weeks and a pattern...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270521
更新日期:1997-05-01 00:00:00
abstract::Monocytes differentiate into dendritic cells (DC) in response to GM-CSF combined with other cytokines including IL-4 and IL-15. Here, we show that IL15-DC are efficient in priming naive CD8+ T cells to differentiate into melanoma antigen-specific cytotoxic T lymphocytes (CTL). While both melanoma peptide-pulsed IL15-D...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636329
更新日期:2007-06-01 00:00:00
abstract::Age-associated thymic involution results in a diminished capacity to regenerate T cell populations, although the magnitude of this effect is unknown. In this report, thymic function was studied in aged vs. young adult mice after lethal irradiation and administration of T cell-depleted bone marrow (BM) from young mice....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199806)28:06<1886::AID-IMM
更新日期:1998-06-01 00:00:00
abstract::Antigen-presenting macrophages (M phi) were derived from day 7 cultures of bone marrow stem cells using L cell conditioned medium. The adherent bone marrow-derived macrophages (BMM phi) were 100% esterase-positive, 95% positive for C3 receptors, 93% positive for Fc receptors, and 95% actively phagocytic. Indirect immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121202
更新日期:1982-12-01 00:00:00
abstract::Immunization leads to the generation of both antibody-forming cells (AFC) and memory B cells which are thought to arise in germinal centers within lymphoid follicles. The findings that the precursors to memory B cells reside in the J11Dlo subpopulation of the spleens in non-immune mice and that this subpopulation is d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220526
更新日期:1992-05-01 00:00:00
abstract::Mucosa-associated lymphoid tissue 1 (Malt1) regulates immune cell function by mediating the activation of nuclear factor κB (NF-κB) signaling through both its adaptor and proteolytic function. Malt1 is also a target of its own protease activity and this self-cleavage further contributes to NF-κB activity. Until now, t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847597
更新日期:2018-10-01 00:00:00
abstract::The endoplasmic reticulum (ER) protein tapasin is essential for the loading of high-affinity peptides onto MHC class I molecules. It mediates peptide editing, i.e. the binding of peptides of successively higher affinity until class I molecules pass ER quality control and exit to the cell surface. The molecular mechani...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939342
更新日期:2010-01-01 00:00:00
abstract::The effect of phorbol 12-myristate 13-acetate (PMA) on the synthesis, assembly and processing of the components of the T cell receptor (TcR) was studied with special focus on the CD3 omega chain. Treatment of the human leukemic T cell line Jurkat with PMA increased the synthesis of the Ti alpha, CD3 gamma and CG3 zeta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230625
更新日期:1993-06-01 00:00:00
abstract::The adoptive transfer of CD4(+)CD25(+) natural regulatory T cells (Treg) is a promising strategy for the treatment of autoimmune diseases and the prevention of alloresponses after transplantation. Clinical trials exploring this strategy require efficient in vitro expansion of this rare cell population. Protocols devel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838904
更新日期:2009-04-01 00:00:00
abstract::Beside diabetes, non-obese diabetic (NOD) mice develop sporadic lymphoid infiltration of the thyroid gland, mimicking Hashimoto's thyroiditis. We have examined the prevalence of those manifestations in NOD mice, the influence of the major histocompatibility complex (MHC) and the association with autoantibodies. The in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271117
更新日期:1997-11-01 00:00:00
abstract::The B cell line 721.174 has lost the ability to present intracellular antigens to major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTL). This phenotype results from a homozygous deletion in the MHC that includes the peptide transporter genes TAP1 and TAP2, and the proteasome subunits ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250238
更新日期:1995-02-01 00:00:00
abstract::Mice carrying the nude mutation (nu/nu) lack a functioning thymus and do not contain detectable levels of immunocompetent T cells. We now report that nu/nu mice to have lymphocytes which can be activated in vivo by heterologous erythrocytes and a Lyt-1 T cell-derived factor (interleukin 2) to generate T helper cells. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100912
更新日期:1980-09-01 00:00:00
abstract::Gene targeting in hybridoma cells provides a tool for generating chimeric antibodies with great ease and at high yield. We present an evaluation of integration vectors for the chimerization of the immunoglobulin heavy chain locus which are universally applicable to hybridomas of different isotypes and mouse strains. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250325
更新日期:1995-03-01 00:00:00
abstract::The structure of two T-immunogenic peptides, one from the gag p24 protein of the human immunodeficiency virus, the other from the 11.1 gene product of Plasmodium falciparum, was studied by circular dichroism spectroscopy in various pH and solvent conditions. Although both sequences are predicted to adopt an alpha-heli...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191033
更新日期:1989-10-01 00:00:00
abstract::Tissue macrophages and their precursors-the blood monocytes-respond rapidly to a bacterial infection with the release of inflammatory mediators. These mediators are involved in the recruitment of phagocytic cells, principally neutrophils, from the blood to the site of infection. To initiate this process macrophages an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250545
更新日期:1995-05-01 00:00:00
abstract::The zinc-finger transcription factor GATA3 serves as a master regulator of T-helper-2 (Th2) differentiation by inducing expression of the Th2 cytokines IL-4, IL-5 and IL-13 and by suppressing Th1 development. Here, we investigated how GATA3 affects Th17 differentiation, using transgenic mice with enforced GATA3 expres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737840
更新日期:2008-09-01 00:00:00
abstract::Good responder (C57/Br/cd) and poor responder (C57BL/10) mice were immunized with mouse thyroid extract in Freund's complete adjuvant. The good responder mice first showed antibody to thyroglobulin on the seventh day while the poor responder animals had slightly lower titers and antibody did not appear until day 12. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830070110
更新日期:1977-01-01 00:00:00
abstract::CD22 is a B lymphocyte-specific membrane protein that functions as an adhesion molecule via its interactions with a subset of alpha 2-6-linked sialic acid-containing glycoproteins. Engagement of CD22 with a monoclonal antibody (HB22.23) that blocks the binding of CD22 to its ligands results in rapid CD22 tyrosine phos...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260610
更新日期:1996-06-01 00:00:00
abstract::Adoptive T-cell therapy (ATCT) can result in tumor rejection, yet the behavior and fate of the introduced T cells remain unclear. We developed a novel bioluminescence mouse model, which enabled highly sensitive detection of T-cell signals at the single-cell level. Transferred T cells preferentially accumulated within ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141452
更新日期:2011-11-01 00:00:00
abstract::MRL/MP-lpr/lpr mice are homozygous for the lpr mutation that results in the accumulation of phenotypically abnormal cells (CD3+CD4+CD8-) in all lymphoid issues. Although no major abnormalities in the T cell receptor repertoire expressed by such lpr cells have been reported, the lpr mutation is a major disease-accelera...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220432
更新日期:1992-04-01 00:00:00
abstract::A majority of immunodominant epitopes of mycobacterial antigens are known to be recognized by murine T cells in the context of several H-2 haplotypes. In this study, we established the frequency of T cells able to recognize these peptides promiscuously, i.e. in the context of allogeneic antigen-presenting cells, using...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251009
更新日期:1995-10-01 00:00:00