Abstract:
:Adoptive T-cell therapy (ATCT) can result in tumor rejection, yet the behavior and fate of the introduced T cells remain unclear. We developed a novel bioluminescence mouse model, which enabled highly sensitive detection of T-cell signals at the single-cell level. Transferred T cells preferentially accumulated within antigen-positive tumors, relative to the unaffected areas in each mouse, and remarkably, expanded within both lymphopenic and P14 mice. This expansion was controlled and efficient, as evaluated by bioluminescence imaging (BLI) of the T-cell signals and by tumor rejection respectively. Analysis of the population dynamics of transferred T cells in ATCT of large tumors revealed that proliferation did not always follow a simple linear pattern of expansion, but showed an oscillating pattern of expansion and contraction that was often followed by a rebound, until full tumor rejection was achieved. Furthermore, visualizing the recall response showed that the transferred T cells responded expeditiously, indicating the ability of these cells to survive, establish memory and compete with endogenous T cells for as long as 1 year after rejecting the tumor.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Charo J,Perez C,Buschow C,Jukica A,Czeh M,Blankenstein Tdoi
10.1002/eji.201141452subject
Has Abstractpub_date
2011-11-01 00:00:00pages
3187-97issue
11eissn
0014-2980issn
1521-4141journal_volume
41pub_type
杂志文章abstract::Intrathymic deletion of immature thymocytes that express self-reactive TCR specificities is essential in the generation of self tolerance. Medullary thymic epithelial cells (mTEC) expressing the transcriptional regulator Aire play a key role in this process by regulating expression of tissue-restricted antigens to ens...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200738052
更新日期:2008-04-01 00:00:00
abstract::Specific monoclonal antibodies (mAb) directed against the CD23 antigen were used to study human interleukin 4 (hIL4)-induced IgE production by blood and tonsillar mononuclear cells. Both peripheral blood and tonsillar mononuclear cells stimulated by hIL4 expressed membrane CD23 as detected by the binding of all anti-C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200120
更新日期:1990-01-01 00:00:00
abstract::The transferrin receptor (TfR) was recently proposed as putative natural killer (NK) cell target structure. Here data are presented against this hypothesis and it is shown that low TfR expression and high NK sensitivity can occur concommitantly . K562 cells were studied at various stages of cell proliferation. No chan...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140412
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220529
更新日期:1992-05-01 00:00:00
abstract::Ptak and Askenase showed that both alphabeta and gammadelta cells are required for transfer of contact sensitivity (CS). This study confirms that day 4 immune cells depleted of gammadelta cells fail to transfer CS to trinitrochlorobenzene (TNP-Cl) systemically and demonstrates that administration of anti-gammadelta mo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270131
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abstract::Mice carrying the H-2b and H-2s haplotypes are genetically nonresponsive to hen egg-white lysozyme (HEL). Analysis of the anti-HEL response patterns of F1, F2 and backcross progeny showed that responsiveness was dominant and H-2 linked. From plaque-forming cell and serum assays in intra-H-2 recombinant mice, it was es...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100513
更新日期:1980-05-01 00:00:00
abstract::The responses of resting human B lymphocytes to a variety of activation signals were studied. Human tonsillar B lymphocytes were separated according to size by countercurrent elutriation. The small B lymphocytes were then stimulated in vitro with various concentrations of anti-mu antibody in the presence or absence of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140203
更新日期:1984-02-01 00:00:00
abstract::Persistent polyclonal B cell lymphocytosis (PPBL) is a hematological disorder diagnosed predominantly in women, characterized by a polyclonal increase in the number of peripheral blood B lymphocytes. Abnormality of the B cell population was evidenced by the finding of multiple bcl-2/Ig gene rearrangements and an addit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200212)32:12<3678::AID-IMMU3678>
更新日期:2002-12-01 00:00:00
abstract::Natural killer (NK) cell activity can be stimulated by different surface receptors. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating human NK cell cytotoxicity and cytokine production. Here we show that stimulation of human NK cells by anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636146
更新日期:2006-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120804
更新日期:1982-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200002)30:2<705::AID-IMMU705>3.0
更新日期:2000-02-01 00:00:00
abstract::We have previously demonstrated that mycobacterial lipoproteins engage TLR2 on human CD4(+) T cells and upregulate TCR-triggered IFN-γ secretion and cell proliferation in vitro. Here we examined the role of CD4(+) T-cell-expressed TLR2 in Mycobacterium tuberculosis (MTB) Ag-specific T-cell priming and in protection ag...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344100
更新日期:2014-05-01 00:00:00
abstract::The distribution of the murine receptor for interleukin (IL) 6 was examined on a variety of cells. Binding sites for IL 6 were found on many cell lines including fibroblasts and bone marrow-derived macrophages. The highest density of binding sites was found on B cell hybridomas and plasmacytomas, irrespective of their...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191121
更新日期:1989-11-01 00:00:00
abstract::X-linked agammaglobulinemia (XLA) is a humoral immunodeficiency disease in man, characterized by an arrest in B lymphocyte differentiation at the precursor B cell stage. The structure of expressed immunoglobulin (Ig) kappa light (L) chain rearrangements of nine B lymphoblastoid cell lines from one XLA patient was inve...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230306
更新日期:1993-03-01 00:00:00
abstract::A novel surface molecule, Tp90, is described which appears to be involved in an antigen-independent pathway of human T lymphocyte activation. The Tp90 molecule was identified by a monoclonal antibody (mAb), MX20, obtained from a fusion using spleen cells of a mouse immunized with cells from two T cell leukemia lines, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170616
更新日期:1987-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190102
更新日期:1989-01-01 00:00:00
abstract::The concept that naive CD4+ and CD8+ T cells require co-stimulatory signals for activation and proliferation is well documented. Less clear is the need for co-stimulation during the effector phase of the T cell response. Here we examined the influence of B7-1 (CD80) during the effector phase of an autoimmune response ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<949::AID-IMMU
更新日期:1998-03-01 00:00:00
abstract::Genetic control has been studied of the response to the encephalitogenic nonapeptide (NP) determinant of myelin basic protein (BP) in inbred guinea pigs of strains resistant or susceptible to induction of experimental autoimmune encephalomyelitis (EAE). By studying bone marrow-reconstituted animals, we found that susc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110409
更新日期:1981-04-01 00:00:00
abstract::MHC-peptide-specific Fab antibodies binding to HLA-A*0201 complexes presenting the wild-type EAAGIGILTV (EAA) or analogue Melan-A 10-mer ELAGIGILTV (ELA) peptide were generated to study efficacy of peptide processing and presentation. None of the selected Fab antibodies detected the naturally processed EAA/HLA-A*0201 ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636545
更新日期:2007-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535615
更新日期:2006-07-01 00:00:00
abstract::Tertiary lymphoid organs can form at sites of chronic inflammation. Their presence has been mainly associated with tissue destruction. In transplantation, there is a dynamic immune response as in chronic inflammation. Indeed, the presence of tertiary lymphoid organs has been associated with chronic rejection. In addit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040759
更新日期:2011-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150514
更新日期:1985-05-01 00:00:00
abstract::Disseminated infections by the opportunistic yeast Cryptococcus neoformans are characterized by accumulation in tissues of glucuronoxylomannan (GXM), the major component of the capsular polysaccharide. We investigated binding, uptake, and disposal of GXM by peripheral blood neutrophils and monocytes, and the effect of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323388
更新日期:2003-04-01 00:00:00
abstract::The invariant chain (Ii) binds to newly synthesized major histocompatibility complex (MHC) class II molecules and is targeted to an acidic compartment where it is degraded. To evaluate its role on the conformation and the subcellular distribution of murine MHC class II molecules we have established stable L cell trans...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231218
更新日期:1993-12-01 00:00:00
abstract::Signaling via the IL-7 receptor complex (IL-7Ralpha/CD127 and IL-2Rgamma/CD132) is required for T-cell development and survival. Decreased CD127 expression has been associated with persistent viral infections (e.g. HIV, HCV) and cancer. Many IL-2Rgamma-sharing (gammaC) cytokines decrease CD127 expression on CD4+ and C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940093
更新日期:2010-05-01 00:00:00
abstract::Cells expressing the chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) and the chemokine C receptor (CCR)4 were consistently detected in the circulation of healthy subjects, whereas numbers of cells expressing CCR3 were much lower. While all CCR4+ cells were T cells, a small proportion of CRT...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200010)30:10<2972::AID-IMMU2972>
更新日期:2000-10-01 00:00:00
abstract::The tendency of isolated germinal center (GC) B cells to undergo apoptosis was suppressed by recombinant cell-bound CD40 ligand (CD40L): after 2 days at 37 degrees C, > 80% of cells remained viable in the presence of CD40L as compared to < 1% in control cultures. CD40L sustained a high rate of DNA synthesis in GC cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230948
更新日期:1993-09-01 00:00:00
abstract::The mer receptor tyrosine kinase mediates phagocytosis of apoptotic cells and modulates cytokine production; it is also required for prevention of systemic autoimmune disease. Using a mer-specific antibody, we have confirmed the presence of mer on macrophages and now report its expression on NK cells, NKT cells, and d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324076
更新日期:2003-08-01 00:00:00
abstract::Single-positive thymocytes are the immediate precursors of peripheral recent thymic emigrants (RTE) which develop into mature peripheral T cells. The functional ability of RTE is unclear but their state of differentiation may be relevant to the development of tolerance to peripheral "self" antigens. Since RTE are diff...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240738
更新日期:1994-07-01 00:00:00
abstract::Co-infection of HCV with HIV has been associated with more rapid progression of HCV-related disease. HCV-specific T-cell immune responses, which are essential for disease control, are attenuated in co-infection with HIV. T-cell exhaustion has recently been implicated in the deficient control of chronic viral infection...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040340
更新日期:2010-09-01 00:00:00