Abstract:
:A commonly held postulate regarding the etiology of rheumatoid arthritis (RA) is that of antigenic mimicry. Recent interest has focused on the mycobacterial 65-kDa heat-shock protein (hsp) as a putative causal agent. The 65-kDa hsp has over 40% sequence homology with the human hsp 60, and elevated synovial T cell responses to both antigens have been demonstrated in RA and juvenile rheumatoid arthritis patients. Such T cells should, therefore, be specific for shared epitopes on the two antigens. To investigate this, we screened synovial fluid mononuclear cells from two early RA patients with peptides of the 65-kDa hsp which have the greatest homology with the human hsp 60. We also raised a panel of T cell clones from one of the patients with the 65-kDa hsp. The synovial T cell population from both patients and one of the T cell clones recognized a peptide representing the amino-acid sequence 241-255. This clone also responded to the peptide of the equivalent human sequence, and was restricted by HLA-DQ. A second T cell clone recognized an adjacent epitope (amino acid sequence 251-265) which is also highly homologous with the human sequence, but this clone was restricted by HLA-DR. The clones utilized different V beta gene segments but the same D beta and J beta gene elements, and both exhibited specific cytotoxicity against autologous antigen-pulsed macrophages. Our findings, therefore, do not disagree with the postulate that autoimmune disease could possibly be triggered by bacterial epitopes with homology to self protein. However, it is also noted that there are alternative interpretations of this data.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Quayle AJ,Wilson KB,Li SG,Kjeldsen-Kragh J,Oftung F,Shinnick T,Sioud M,Førre O,Capra JD,Natvig JBdoi
10.1002/eji.1830220529keywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
1315-22issue
5eissn
0014-2980issn
1521-4141journal_volume
22pub_type
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2007-04-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1982-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171209
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journal_title:European journal of immunology
pub_type: 评论,杂志文章
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journal_title:European journal of immunology
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pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
abstract::Th cells can adopt a number of different phenotypes. We performed microarray-assisted mRNA profiling on antigen-stimulated, TCR transgenic murine splenocytes that were cultured in the presence of cytokines. Transcriptome snapshots of Th cells differentiating into Th1 and Th2 phenotypes were obtained at various time po...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242979
更新日期:2013-04-01 00:00:00
abstract::An experimental model of adenosine deaminase deficiency was established on the human T cell line Jurkat by using 2'-deoxycoformycin, a strong specific inhibitor of the enzyme. When deoxyadenosine was added to the inhibited cells, the nucleotide profile was modified reproducing that found in lymphocytes from adenosine ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200322
更新日期:1990-03-01 00:00:00
abstract::Indoleamine 2,3-dioxygenase (IDO) suppresses T cell responses by its action in catabolising tryptophan. It is important in maintenance of immune privilege in the placenta. We investigated the activity of IDO in the cornea, following corneal transplantation and the effect of IDO over-expression in donor corneal endothe...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100705
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journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
abstract::Single-positive thymocytes are the immediate precursors of peripheral recent thymic emigrants (RTE) which develop into mature peripheral T cells. The functional ability of RTE is unclear but their state of differentiation may be relevant to the development of tolerance to peripheral "self" antigens. Since RTE are diff...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240738
更新日期:1994-07-01 00:00:00
abstract::This is the first report on the establishment of the readily identifiable and functioning thymic nurse cell (TNC) clones from the mouse thymus. In the course of the culture of an epithelial cell line using a medium with a low concentration of Ca2+ from the spontaneous BALB/c thymic tumor, lymphocytes as well as thymic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180525
更新日期:1988-05-01 00:00:00