Abstract：
:Heat-stable antigen (HSA)/mouse CD24 (formerly termed Nectadrin) is a membrane glycoprotein with an unusual structure consisting of a small protein core and extensive glycosylation. It is expressed by hematopoietic cells but not by mature T lymphocytes. HSA on accessory cells is an important costimulatory molecule required for the clonal expansion of T lymphocytes. HSA is also involved in cell-cell adhesion events and the isolated antigen has been shown to possess self-binding properties. In the present study we have re-investigated the role of HSA in T cell proliferation. We find that following stimulation of T lymphocytes with concanavalin A or of CD4+ T lymphocytes with a combination of anti-CD3/CD28 monoclonal antibodies (mAb) the HSA antigen is transiently expressed. The expression correlated with the appearance of CD25 and a CD2 activation epitope at the cell surface. Induction of HSA was also seen in vivo on V beta 8+ T lymphocytes in BALB/c mice that were injected with Staphylococcal enterotoxin B. Biosynthetic labeling and analysis of mRNA by reverse transcriptase-polymerase chain reaction showed that HSA was synthesized by activated T lymphocytes. A combination of anti-CD3 and mAb 79 to HSA was incapable of inducing proliferation of purified CD4+ T lymphocytes. However, the antibody strongly enhanced the response obtained with a combination of anti-CD3/CD28 mAb. The augmenting effect of the HSA-specific mAb was dose dependent. Since HSA is bound to the membrane via a glycosyl-phosphatidylinositol (GPI) anchor and GPI-anchored molecules have been implicated in lymphocyte activation, it is conceivable that HSA is not only a costimulatory molecule on accessory cells but is also a signaling molecule in T lymphocytes. The possibility of a homotypic HSA/HSA interaction between T lymphocytes and accessory cells is discussed.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Hubbe M,Altevogt Pdoi
10.1002/eji.1830240336subject
Has Abstractpub_date
1994-03-01 00:00:00pages
731-7issue
3eissn
0014-2980issn
1521-4141journal_volume
24pub_type
杂志文章abstract：:Indirect immunofluorescence revealed that 13% of BALB/c and 33% of CBA spleen cells of B type carry specific binding sites at their surface for double-stranded poly(I).poly(C). Pretreatment of BALB/c spleen cells with anti-mouse immunoglobulin serum increased the number of B cells capable of binding poly(I).poly(C) in...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830081213
更新日期：1978-12-01 00:00:00
abstract：:The expression of IgG Fc receptor (FcR) molecules was examined on Ly-1+ B cells and B cells tumors. FcR molecules were found on a representative Ly-1+ B cell lymphoma of the pre-B and as well as of the mature cell B phenotypes. The expression of the FcR did not change on these cells during their differentiation to B c...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830170621
更新日期：1987-06-01 00:00:00
abstract：:The human butyrophilin (BTN) 3 or CD277 molecules belong to the B7 family members and are expressed in various immune cells such as T and NK cells. Here, we show that CD277 triggering considerably enhances TCR-induced cytokine production and cell proliferation, even when another co-stimulatory molecule, CD28, is engag...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.201141404
更新日期：2011-12-01 00:00:00
abstract：:The internalization and intracellular processing of monoclonal antibody to immunoglobulin mu heavy chain (Mamu) have been investigated in two human Burkitt lymphoma cell lines (Ramos and Raji), in a human B cell lymphoma and in normal human peripheral blood B cells. In addition to the degradation of 125I-labeled Mamu ...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830160314
更新日期：1986-03-01 00:00:00
abstract：:Antisera were prepared in rabbits against the idiotypic (Id) determinants of antiallergen antibodies. These antibodies were isolated from the plasma of 10 unrelated patients by immunoabsorption. Three major allergens isolated from the house dust mite, Dermatophagoides pteronyssinus (DPT), were used. The anti-Id antise...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830160520
更新日期：1986-05-01 00:00:00
abstract：:The major role of cells of the dendritic family in immunity and tolerance has been amply documented. Since their discovery in 1973, these cells have gained increasing interest from immunologists, as they are able to detect infectious agents, migrate to secondary lymphoid tissue, and prime naive T lymphocytes, thereby ...
journal_title：European journal of immunology
pub_type： 杂志文章,评审
doi：10.1002/eji.201242480
更新日期：2012-10-01 00:00:00
abstract：:Treatment of systemic lupus erythematosus patients with epratuzumab (Emab), a humanized monoclonal antibody targeting CD22, leads to moderately reduced B-cell numbers but does not completely deplete B cells. Emab appears to induce immunomodulation of B cells, but the exact mode of action has not been defined. In the p...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.201646383
更新日期：2016-09-01 00:00:00
abstract：:Multi-component glycopeptide libraries and single glycopeptides were used for immunization of mice with the aim of inducing strong T helper cell responses to the repetitive sequence of MUC1 expressed by human tumor cells. The glycopeptides and glycopeptide libraries were modeled upon the native human MUC1 amino acid v...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200323698
更新日期：2003-06-01 00:00:00
abstract：:Among 15 anti-DNA antibody-producing hybridomas derived from a single NZB X NZW F1 mouse, an IgM and an IgG were shown to use the same VH gene of the Q52 family. Using a combination of two primers both consisting of a mixture of oligonucleotides (one complementary to the 5' end of VH segment and one to the 3' end of V...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830220417
更新日期：1992-04-01 00:00:00
abstract：:Respiratory syncytial virus (RSV) causes severe bronchiolitis in infants worldwide. The immunological factors responsible for RSV susceptibility in infants are poorly understood. Here, we used the BALB/c mouse model of neonatal RSV infection to study the mechanisms leading to severe disease upon reexposure to the viru...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.201545929
更新日期：2016-04-01 00:00:00
abstract：:The immune and endocrine systems ensure two vital functions in the body. The immune system protects us from lethal pathogens, whereas the endocrine system ensures proper metabolic function of peripheral organs by regulating systemic homeostasis. These two systems were long thought to operate independently. The immune ...
journal_title：European journal of immunology
pub_type： 杂志文章,评审
doi：10.1002/eji.201847895
更新日期：2019-07-01 00:00:00
abstract：:Human peripheral blood mononuclear leukocytes (PBL) cocultured with WISH human amnion cells rapidly produced alpha-type interferon (IFN-alpha) and in an IFN-dependent manner protected the WISH cells against the cytopathic effects caused by vesicular stomatitis virus. When the number of PBL per WISH cell monolayer cult...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830120515
更新日期：1982-05-01 00:00:00
abstract：:The glycolipid alpha-galactosylceramide (alpha-GalCer) is a potent activator of invariant natural killer T (iNKT) cells and has been shown to be an effective agent against cancer, infections and autoimmune diseases. The effectiveness of alpha-GalCer and its alkyl chain analogues depends on efficient loading and presen...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200737518
更新日期：2008-03-01 00:00:00
abstract：:IL-23, a proximal regulator of IL-17, may be a major driving force in the induction of autoimmune inflammation. We have used a model of subdiabetogenic treatment with multiple low doses of streptozotocin (MLD-STZ; 4 x 40 mg/kg body weight) in male C57BL/6 mice to study the effect of IL-23 on immune-mediated beta cell ...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200535325
更新日期：2006-01-01 00:00:00
abstract：:Interleukin-12 (IL-12) and Flt3 ligand (FL) regulate hematopoiesis by stimulating proliferation, differentiation and migration of progenitor and/or stem cells. In this study, we evaluated the in vivo effect of IL-12 alone or in combination with FL on dendritic cell (DC) generation and accumulation in murine spleen, ly...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/1521-4141(200009)30:9<2565::AID-IMMU2565>3
更新日期：2000-09-01 00:00:00
abstract：:We have studied a secondary IgG anti-dinitrophenyl response in dissociated mouse spleen cell cultures which is comparable in magnitude to the responses detected after adoptive transfer. The data indicate that the responsive precursor cell is not a recirculating memory cell but is an intermediate B cell type which appe...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830060104
更新日期：1976-01-01 00:00:00
abstract：:Human plasmacytoid dendritic cells (PDC) are believed to link innate and adaptive immunity by producing type I interferon (IFN-I) and triggering adaptive T cell-mediated immunity. However, it remains elusive to which degree both PDC functions are linked. Here we show that CMV antigen targeted to PDC using a CD303 (blo...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200737552
更新日期：2008-07-01 00:00:00
abstract：:In man, B cell maturation factors obtained from T cells or T cell lines have been shown to induce antibody formation in mitogen or anti-immunoglobulin activated B cells, and in some continuous B cell lines, but the relationships between these factors and B cell differentiation factors in antigen-specific antibody resp...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830150111
更新日期：1985-01-01 00:00:00
abstract：:Indirect immunofluorescence performed with 2 polyclonal and 4 monoclonal antibodies directed against human beta 2-microglobulin (beta 2m) revealed the presence of a beta 2m-like determinant on the membrane of a subpopulation of earthworm leukocytes classified as weakly adherent acidophils. Two out of the 4 monoclonal ...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830131216
更新日期：1983-12-01 00:00:00
abstract：:We have established IgG rheumatoid factor (RF)-secreting hybridoma cell lines from the synovial tissues of three patients from whom we have previously characterized several IgM RF. The IgG RF bind human and rabbit IgG and form intracellular complement-fixing complexes indicative of a self association process in vivo. ...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830230604
更新日期：1993-06-01 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSC) play an important role in the cellular network regulating immune responses in cancer, chronic infectious diseases, autoimmunity, and in other pathological conditions. Morphological, phenotypic and functional heterogeneity is a hallmark of MDSC. This heterogeneity demonstrates th...
journal_title：European journal of immunology
pub_type： 杂志文章,评审
doi：10.1002/eji.201040895
更新日期：2010-11-01 00:00:00
abstract：:AMPK (adenosine monophosphate-activated protein kinase) is phosphorylated (AMPK-P) in response to low energy through allosteric activation by Adenosine mono- or diphosphate (AMP/ADP). Folliculin (FLCN) and the FLCN-interacting proteins 1 and 2 (FNIP1, 2) modulate AMPK. FNIP1 deficiency patients have a AMPK-P gain of f...
journal_title：European journal of immunology
pub_type： 信件
doi：10.1002/eji.201948504
更新日期：2020-07-01 00:00:00
abstract：:The immunocytochemical characteristics of large granular lymphocytes (LGL), isolated from the liver sinusoids of euthymic and athymic (nude) rats, were investigated in electron microscopy by the immunoperoxidase technique. The LGL were found positive for MRC OX-8 (natural killer cells and cytotoxic/suppressor T cells)...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830171006
更新日期：1987-10-01 00:00:00
abstract：:The thymus is a primary lymphoid organ supporting the development of self-tolerant T cells. Key events in T-cell development in the thymus include lineage commitment, selection events, and thymic emigration. This review discusses the proposed role of sphingosine-1-phosphate and its receptors in the emigration of both ...
journal_title：European journal of immunology
pub_type： 杂志文章,评审
doi：10.1002/eji.200838912
更新日期：2009-04-01 00:00:00
abstract：:The Fas antigen (Fas), which is a cell surface protein belonging to the tumor necrosis factor receptor family, mediates apoptosis. To assess the contribution of Fas to the pathogenesis of retrovirus-induced immunodeficiency, we examined the kinetics of Fas expression on the lymphocytes during the course of murine acqu...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830241028
更新日期：1994-10-01 00:00:00
abstract：:Sporozoite surface protein 2 has been identified as a target of malaria vaccines designed to produce protective CD8+ cytotoxic T lymphocytes (CTL) because mice immunized with mastocytoma cells expressing a fragment of Plasmodium yoelii sporozoite surface protein 2 (PySSP2) are protected against malaria by an immune re...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830240705
更新日期：1994-07-01 00:00:00
abstract：:The present experiments address functional antibody diversity and clonal distribution in murine available repertoires. IgM-containing supernatants were prepared by unbiased, polyclonal stimulation of resting splenic B cells from C57BL/6 mice, to ensure similar numbers of responding clones/culture and equivalent growth...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/(SICI)1521-4141(199804)28:04<1204::AID-IMM
更新日期：1998-04-01 00:00:00
abstract：:We have investigated perforin and granzyme B expression in graft-infiltrating lymphocytes of patients who underwent heart transplantation. Those proteins are commonly present in the cytoplasmic granules of cytotoxic T lymphocytes and are released upon effector-target cell interaction. From 28 patients 103 endomyocardi...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830240136
更新日期：1994-01-01 00:00:00
abstract：:Atopic dermatitis (AD) or eczema is the most common chronic inflammatory skin disease. It is a multifactorial disease with local and systemic immune changes. Current therapies focus on restoring the local skin barrier or inhibiting immune responses. In this issue of the European Journal of Immunology, Sehra et al. [Eu...
journal_title：European journal of immunology
pub_type： 评论,杂志文章
doi：10.1002/eji.201646708
更新日期：2016-11-01 00:00:00
abstract：:We here report the cDNA sequences of 11 new rat Ly49 genes with full and three with incomplete open reading frames. Although obtained from different inbred rat strains, these as well as six previously published cDNA represent non-allelic genes matching different loci in the Brown Norway (BN) rat genome, which is predi...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200425429
更新日期：2005-01-01 00:00:00
