Abstract:
:Indirect immunofluorescence performed with 2 polyclonal and 4 monoclonal antibodies directed against human beta 2-microglobulin (beta 2m) revealed the presence of a beta 2m-like determinant on the membrane of a subpopulation of earthworm leukocytes classified as weakly adherent acidophils. Two out of the 4 monoclonal antibodies were found to bind to 15% of the earthworm leukocytes. Similar results were obtained with antibodies specific for beta 2m and isolated by affinity chromatography from either polyclonal antiserum or anti-beta 2m hybridoma supernatant. Additional binding inhibition and competition experiments strengthened the results and suggested that the homology between the structure present on earthworm leukocytes and human beta 2m may be restricted to only one major determinant. The existence of a beta 2m-like molecule in earthworms may provide important clues for understanding protein evolution and basic immune mechanisms.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Roch P,Cooper EL,Eskinazi DPdoi
10.1002/eji.1830131216subject
Has Abstractpub_date
1983-12-01 00:00:00pages
1037-42issue
12eissn
0014-2980issn
1521-4141journal_volume
13pub_type
杂志文章abstract::A series of rat monoclonal antibodies (mAb) directed against the Thy-1 molecule have been evaluated for their ability to stimulate immature (CD4-8-) murine thymocytes. CD4-8- thymocytes could be induced to proliferate by several anti-Thy-1 mAb provided that exogenous interleukin 1 or interleukin 2 was provided. Appare...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171127
更新日期:1987-11-01 00:00:00
abstract::In this study we examined the effect of interleukin 4 (IL 4) on T cell activation and proliferation via the alternative CD2 pathway. To this end highly purified human resting T cells were cultured with a stimulating pair of anti-CD2 monoclonal antibodies in the absence of accessory signals from monocytes. Addition of ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200724
更新日期:1990-07-01 00:00:00
abstract::The mechanisms responsible for the down-modulation of the activation of separated CD4(+) or CD8(+) human T cell blasts were studied using cells obtained from healthy donors. In the presence of IL-2, human CD8(+) T cell blasts were more sensitive than CD4(+) T cell blasts to regulation by APO2 ligand/TNF-related apopto...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526046
更新日期:2005-06-01 00:00:00
abstract::Current concepts of activation of the alternative pathway of complement (APC) focus on the central role of an amplification mechanism triggered by C3b which is covalently bound to the surfact of activating substances. Using sulfated polyanions as model substances, an efficient fluid-phase activation of complement is d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110405
更新日期:1981-04-01 00:00:00
abstract::Two cloned murine helper T lymphocyte (HTL) lines were used to investigate the immunoregulatory properties of highly purified interleukin 2 (IL2). The clone, designated J6.19, secretes lymphokines, including IL2, and proliferates when stimulated with ovalbumin in the presence of I-Ak-bearing spleen cells, while the al...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160302
更新日期:1986-03-01 00:00:00
abstract::To study the control of immunoglobulin kappa light chain gene rearrangement, we generated transgenic mice carrying a germ-line human kappa minilocus (HK) containing the J kappa-proximal V gene, V kappa IV, the V-J intergenic region, the five J kappa segments and the C kappa gene. This construct includes the intronic, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::A majority of immunodominant epitopes of mycobacterial antigens are known to be recognized by murine T cells in the context of several H-2 haplotypes. In this study, we established the frequency of T cells able to recognize these peptides promiscuously, i.e. in the context of allogeneic antigen-presenting cells, using...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251009
更新日期:1995-10-01 00:00:00
abstract::The ganglioside GD3 has been described as a membrane component of human T cells which is involved in T cell growth. In the present study the activating function of GD3 for human CD4+ and CD8+ T cells was analyzed by five different monoclonal antibodies (mAb) directed against the GD3 molecule. Three mAb U5, Z21 and R24...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240530
更新日期:1994-05-01 00:00:00
abstract::The migration of immunocytes within the extracellular matrix (ECM) is influenced by the activation state of the incoming cell and its responses to the presence of chemokines and cytokines. We studied the regulatory role of TGF-beta1 on T cell homing to secondary lymphatic organs, such as the spleen, and chemotaxis wit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200201)32:1<193::AID-IMMU193>3.0
更新日期:2002-01-01 00:00:00
abstract::Cells from the monoclonal B cell lymphoma I.29 expressing surface IgM (mu +) are capable of differentiating in vitro to IgM secretion and of switching to IgA or IgE production in response to lipopolysaccharide (LPS) stimulation. To determine whether a single mu + B cell is capable of undertaking both differentiative p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170419
更新日期:1987-04-01 00:00:00
abstract::An association between T-cell lymphopenia and autoimmunity has long been proposed, but it remains to be elucidated whether T-cell lymphopenia affects B-cell responses to autoantigens. Human neonatal thymectomy (Tx) results in a decrease in T-cell numbers and we used this model to study the development of autoreactivit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201746971
更新日期:2017-11-01 00:00:00
abstract::Antigen-induced stimulation of T cells is mediated via the CD3 antigen receptor (Ti) complex and monoclonal antibodies (mAb) reacting with CD3 and Ti result in rapid intracellular Ca2+ mobilization, followed by monocyte-dependent proliferation. Combinations of mAb to CD2, the sheep red blood cell receptor, also mobili...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160521
更新日期:1986-05-01 00:00:00
abstract::A study was made of the ability of the superantigen staphylococcal enterotoxin B (SEB) to induce relapses of experimental allergic encephalomyelitis (EAE) in SJL mice that had partially or completely recovered from acute EAE. We find that a single injection of 0.05 mg SEB i.v. induces mild relapses in 50% of such mice...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251108
更新日期:1995-11-01 00:00:00
abstract::T lymphocytes can be activated and induced to proliferate through stimulation of the CD2 glycoprotein with functional combinations of CD2 antibodies. However, this mechanism of signal transduction via CD2 is still not fully understood. We have investigated which molecules on the T cell surface preferentially associate...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200205)32:5<1509::AID-IMMU1509>3
更新日期:2002-05-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated autoimmune disease inducible in rodents by immunization of brain-specific antigens such as myelin basic protein (MBP). It is also well known that various strains of rats differ in their susceptibility to EAE upon active immunization. To elucidate the...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200934
更新日期:1990-09-01 00:00:00
abstract::The transcription factor c-Maf is critical for IL-4 production and the development of Th2 cells, which promote humoral immunity and protect against extracellular parasites. Yet, little else is known of c-Maf function in CD4 cells. Here, we identify a novel role for c-Maf in regulating susceptibility to apoptosis. Over...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636979
更新日期:2007-10-01 00:00:00
abstract::We have investigated the role of TNF-alpha in mast cell-mediated late airway hyperresponsiveness (AHR) using mast cell-deficient WBB6F1-W/W(v) (W/W(v)) mice in a murine model of asthma, which exhibits a biphasic increase in AHR. TNF-alpha levels in the airway and magnitude of late AHR in response to airway allergen ch...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636612
更新日期:2007-04-01 00:00:00
abstract::Mast cells are important cellular constituents of epithelial-mesenchymal interactions, densely located at sites of microbial entry into the host where they are continuously exposed to products from commensals. In order to avoid excessive activation and the associated pathology, mast cell responses to TLR agonists must...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242786
更新日期:2013-04-01 00:00:00
abstract::The antagonistic effects of macrophage (M phi)-derived interleukin 1 (IL 1) and prostaglandin E2 (PGE2) on thymocyte proliferation were uncoupled and studied. Elimination of PGE2 from the culture medium of prostaglandin E2-stimulated M phi was achieved by dialysis of the media or by indomethacin treatment of the M phi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150109
更新日期:1985-01-01 00:00:00
abstract::The recent discovery of IL-17-producing CD4(+) Th subset significantly revised the Th1/Th2 dichotomy model proposed by Mosmann and Coffman almost two decades ago. Th17 cells are involved in the pathogenesis of many human autoimmune diseases. Th17 cells, their developmental pathways and their effector functions, theref...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839105
更新日期:2009-03-01 00:00:00
abstract::Recent studies have suggested that T cell memory for recall antigens resides in clones of primed T cells with a short inter-mitotic half-life. In humans such cells express an isoform of the leukocyte common antigen termed CD45RO. Nevertheless, little is known of the fate of these primed T cells after initial activatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240417
更新日期:1994-04-01 00:00:00
abstract::T-cell activation and the subsequent transformation of activated T cells into T-cell blasts require profound changes in cell volume. However, the impact of cell volume regulation for T-cell immunology has not been characterized. Here we studied the role of the cell-volume regulating osmolyte transporter Taut for T-cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141690
更新日期:2012-04-01 00:00:00
abstract::Aging is accompanied by altered immunity, resulting in a variable state of poorly understood immunodeficiency. While both the numbers and the functionality of naïve T cells are decreased by aging, the impact of these changes upon immune defense against bacterial pathogens in vivo remains understudied. Using a model of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041141
更新日期:2011-05-01 00:00:00
abstract::The relative complement-mediated lytic capability of the IgG subclass isotypes was studied using a matched set of mouse-human chimeric anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies. The subclass pattern was shown to be highly dependent on variations in antigen concentration and to lesser extent on variation in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210103
更新日期:1991-01-01 00:00:00
abstract::Anti-HLA alloantisera inhibit mixed lymphocyte responses in which normal lymphocytes are used as stimulator cells. These same antisera are unable to inhibit lymphocyte proliferative responses stimulated by lymphoblastoid cells from cultured lymphoid cell lines. They also fail to inhibit either the generation of cytoto...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060402
更新日期:1976-04-01 00:00:00
abstract::Major histocompatibility complex (MHC) class II-associated antigen presentation is mainly linked to processing of exogenous antigens upon cellular uptake by endocytosis, but has also been observed for endogenously synthesized antigens. We have studied the MHC class II-associated presentation of the endogenously synthe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251230
更新日期:1995-12-01 00:00:00
abstract::We have analyzed the T cell receptor (TcR) V alpha and TcR V beta regions in the spontaneous mouse model for insulin-dependent diabetes mellitus, the NOD mouse, and compared it to the regions in the two sister strains, the NON and CTS strains. Based on restriction fragment length polymorphism analysis the TcR V alpha ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220337
更新日期:1992-03-01 00:00:00
abstract::We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747019
更新日期:2017-08-01 00:00:00
abstract::Interleukin-12 (IL-12) has been shown to be produced by monocytes by the ligation of CD40. In the present experiments, IL-12 is shown to be produced by murine spleen antigen-presenting cells (APC) by interaction with T helper 1 (Th1) clones through CD40-CD40 ligand (CD40L) interaction, but not with Th2 clones. The IL-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260318
更新日期:1996-03-01 00:00:00
abstract::Spleen cells from chickens injected with sheep erythrocytes (SE) intravenously 2 to 14 days prior to culture were found to give faster and higher plaque-forming cell responses upon addition of antigen on day 2 rather than on day 0 of culture. Cell mixture experiments showed that this was due to the induction of suppre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100309
更新日期:1980-03-01 00:00:00