Abstract:
:Current concepts of activation of the alternative pathway of complement (APC) focus on the central role of an amplification mechanism triggered by C3b which is covalently bound to the surfact of activating substances. Using sulfated polyanions as model substances, an efficient fluid-phase activation of complement is demonstrated in contrast to solid-phase activation. It is shown that particulate high-molecular weight sulfated polyanions are capable of reversible binding the guinea pig and human regulatory protein beta1H. This fixation leads to an extensive activation of C3 and factor B because the regulatory function of beta1H is blocked in the fluid-phase C3b-dependent amplification system of the APC. Addition of beta1H-depleted C4-deficient guinea pig serum reconstitutes the physiological control mechanisms of the APC. Guinea pig beta1H, purified to homogeneity, is described as a 160000 dalton protein of a single-chain structure. In addition, highly specific and sensitive test systems for beta1H are described.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Bitter-Suermann D,Burger R,Hadding Udoi
10.1002/eji.1830110405subject
Has Abstractpub_date
1981-04-01 00:00:00pages
291-5issue
4eissn
0014-2980issn
1521-4141journal_volume
11pub_type
杂志文章abstract::Fc gamma receptor I-positive (CD64+) and Fc gamma receptor I-negative (CD64-) monocytes were prepared from highly purified (elutriation-derived) human monocytes by cytofluorograph cell sorting, and a phenotypical and functional dissociation of the isolated CD64+ and CD64- monocyte subsets is demonstrated. Surface anal...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231213
更新日期:1993-12-01 00:00:00
abstract::Peptides associated with HLA-B35 commonly have a proline or occasionally a serine residue in the P2 anchor position of the peptide, with a tyrosine at the C terminus. Based on this motif, we identified an octamer epitope from influenza A matrix protein which is presented by HLA-B35. The requirements for MHC binding an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260210
更新日期:1996-02-01 00:00:00
abstract::Transcription of the gene encoding the transcriptional coactivator Oct-binding factor 1 (OBF-1)/OCA-B/Bob.1 is largely restricted to B cells. During B cell development OBF-1 expression shows two peaks, one in immature B cells in the bone marrow and the other in germinal center B cells. Promoter analysis has identified...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323882
更新日期:2003-10-01 00:00:00
abstract::Immunodominant T cell epitopes from the autoantigen human cartilage glycoprotein 39 have previously been mapped in the context of HLA-DR*0401 and *0402, using mice expressing HLA-DR4 transgenes. We measured the dissociation rates of these epitopes from soluble recombinant DR*0401 and DR*0402 to assess the relationship...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200203)32:3<662::AID-IMMU662>3.0
更新日期:2002-03-01 00:00:00
abstract::Plasmodium falciparum stimulates peripheral blood gamma delta + T cells from unexposed donors. The responding cells bear V gamma 9+ chains of the T cell receptor, the majority of which, but not all, are associated with V delta 2 chains. We have analyzed whether the precursor frequency of these V gamma 9+ cells approac...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221045
更新日期:1992-10-01 00:00:00
abstract::Double-negative (DN) thymocyte subsets were examined in mice deficient in the CD3 zeta chain (zeta-/-). The HSA+CD44-CD25- subset was found to be missing, and DN thymocytes seemed to differentiate directly from HSA+CD25+CD44- cells to double-positive (DP) cells. When fetal thymic ontogeny was examined, we found a mark...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240828
更新日期:1994-08-01 00:00:00
abstract::Adoptive lymphocyte transfers between Iga, Igb and Igd allotype-congenic mouse strains revealed host barriers against the production of certain donor allotypes. First, as recipients of Igb cells, Iga and Igd mice permitted the production of donor Ig-4b but not that of Ig-1 b. The apparent mediators of this Ig-1 b barr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830080805
更新日期:1978-08-01 00:00:00
abstract::CBA/N mice have an X-linked immune defect in B lymphocyte function which leads to their inability to respond to several thymus-independent antigens. We report here that these mice and immunologically defective F1 male (CBA/N X DBA/2N) mice can respond to Brucella abortus and to 2,4,6-trinitrophenyl derivatives of Bruc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830080703
更新日期:1978-07-01 00:00:00
abstract::The lymph node cells (LNC) activated in vivo by liver regeneration following partial hepatectomy of mice (primed lymph node cells) respond to regenerating liver cells in vitro with typical secondary immune response characteristics (Miyahara, S. et al., Eur. J. Immunol. 1983. 13: 878). These LNC activated in vivo suppr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131105
更新日期:1983-11-01 00:00:00
abstract::Mast cells are important cellular constituents of epithelial-mesenchymal interactions, densely located at sites of microbial entry into the host where they are continuously exposed to products from commensals. In order to avoid excessive activation and the associated pathology, mast cell responses to TLR agonists must...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242786
更新日期:2013-04-01 00:00:00
abstract::A method is described for achieving the efficient production of proteins of biological interest by the establishment of hybridomas from lymphocytes of transgenic animals carrying a fusion gene having promoter-regulatory sequences functional in lymphocytes fused to the coding sequences of the protein to be produced. Wh...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160829
更新日期:1986-08-01 00:00:00
abstract::Lymphoid follicles of the ileal Peyer's patch (PP) of young sheep function as the major source of B cells and a site of immunoglobulin (Ig) receptor diversification. However, extensive cell death in culture has restricted investigations of ileal PP follicular (iPf)B cell biology. We investigated the possibility that s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240220
更新日期:1994-02-01 00:00:00
abstract::Growing evidence suggests that some immune responses are mediated not only by conventional and distinct NK cells and CTL, but also by T cell subsets expressing NK receptors and NK cell-associated molecules. Consistent with our previously published finding that the mAb 1F8 identifies non-T/non-B cells in Xenopus that e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1574::AID-IMMU1574>3
更新日期:2002-06-01 00:00:00
abstract::Using a model of 2,4-dinitro-1-fluorobenzene-induced contact hypersensitivity (CHS) we found that, as compared with wild-type mice, IL-15 receptor alpha chain (IL-15Ralpha)-deficient mice showed significantly less ear swelling. This decreased response was associated with diminished expression of CCL5/RANTES and CXCL10...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425577
更新日期:2005-03-01 00:00:00
abstract::We have analyzed the T cell receptor (TcR) V alpha and TcR V beta regions in the spontaneous mouse model for insulin-dependent diabetes mellitus, the NOD mouse, and compared it to the regions in the two sister strains, the NON and CTS strains. Based on restriction fragment length polymorphism analysis the TcR V alpha ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220337
更新日期:1992-03-01 00:00:00
abstract::The proteins p40 (Mr = 40 000), p32 (Mr = 32 000), p28 (Mr = 28 000), p20 (Mr = 20 000) and p10 (Mr = 10 000) are described which occur in noncovalent association with the polymorphic alpha, beta heterodimer of class II antigens. They were investigated with respect to their molecular characteristics and their mutual s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140606
更新日期:1984-06-01 00:00:00
abstract::In M54 mice transgenic for a completely rearranged mu(a) heavy chain there is a decrease in total B cells and the rearrangement of endogenous immunoglobulin genes is partially inhibited. Surprisingly, however, endogenous immunoglobulin gene rearrangement and significant heavy chain polypeptide production does occur. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220909
更新日期:1992-09-01 00:00:00
abstract::Stilbene disulfonates are known to competitively inhibit Cl-/HCO3- flux through Band 3-related anion exchange (AE) proteins. To study the role of AE in lymphocyte activation, stilbene disulfonates were added to cultures of rat splenocytes (SPL). Four different stilbene derivatives were tested and each directly stimula...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220509
更新日期:1992-05-01 00:00:00
abstract::Toll-like receptors (TLRs) are playing important roles in stimulating the innate immune response and intensifying adaptive immune response against invading pathogens. Appropriate regulation of TLR activation is important to maintain a balance between preventing tumor activation and inhibiting autoimmunity. Toll-like r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545567
更新日期:2016-03-01 00:00:00
abstract::The binding of antigen to the multicomponent T cell antigen receptor (TcR)-CD3 complex leads to the activation of several signal transduction pathways which result in T lymphocyte proliferation and lymphokine secretion by molecular mechanisms and catalytic molecules as yet poorly defined. One of the earliest events th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220142
更新日期:1992-01-01 00:00:00
abstract::The thymus is a primary lymphoid organ supporting the development of self-tolerant T cells. Key events in T-cell development in the thymus include lineage commitment, selection events, and thymic emigration. This review discusses the proposed role of sphingosine-1-phosphate and its receptors in the emigration of both ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200838912
更新日期:2009-04-01 00:00:00
abstract::Cytomegaloviruses (CMVs) are highly prevalent, persistent human pathogens that not only evade but also shape our immune responses. Natural killer (NK) cells play an important role in the control of CMV and CMVs have in turn developed a plethora of immunoevasion mechanisms targeting NK cells. This complex interplay can...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201646762
更新日期:2018-01-01 00:00:00
abstract::A comparison of the clonal nature of the immune response to phosphorylcholine (PC) was made in nine different inbred mouse strains. Quantitative idiotypic analysis showed that anti-PC antibodies from each strain were composed of antibodies bearing binding-site idiotypic determinants indistinguishable from two differen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830061002
更新日期:1976-10-01 00:00:00
abstract::The functional immunoglobulin (Ig) genes of B lymphocytes undergo somatic mutations during immune responses. These mutations modify the antigen binding site of the immunoglobulins, thereby enhancing the average affinity of the antibodies produced. The molecular mechanism underlying these B cell hypermutations remains ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251210
更新日期:1995-12-01 00:00:00
abstract::Inhibition of leukocyte trafficking via alpha4-integrin antibody blockade has recently become a validated therapeutic approach for several inflammatory diseases, including multiple sclerosis, ulcerative colitis and Crohn's disease. In the midst of this recent success, 3 patients receiving chronic treatment with the an...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200535195
更新日期:2005-08-01 00:00:00
abstract::Using the patch-clamp technique in combination with fluorescence microscopy we have found an abnormality in voltage-gated K+ channel expression in T cells that represents the first molecular marker linking three disparate autoimmune diseases in mice. CD4-CD8-Thy-1.2+ (double-negative or DN) lymphocytes from every know...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200406
更新日期:1990-04-01 00:00:00
abstract::Intrathymic deletion of immature thymocytes that express self-reactive TCR specificities is essential in the generation of self tolerance. Medullary thymic epithelial cells (mTEC) expressing the transcriptional regulator Aire play a key role in this process by regulating expression of tissue-restricted antigens to ens...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200738052
更新日期:2008-04-01 00:00:00
abstract::CD82, a member of tetraspan family (tetraspanin), is a multifunctional molecule that is involved in cell activation, costimulation, and cell spreading of T cells. Here we show that immobilized anti-CD82 monoclonal antibody (mAb) as well as anti-alpha4beta1 integrin mAb induced tyrosine phosphorylation of pp105/Crk-ass...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200205)32:5<1328::AID-IMMU1328>3
更新日期:2002-05-01 00:00:00
abstract::NK cells are the main cells of the innate immune system that produce IFN-γ, and they express this cytokine at early stages of maturation in response to cytokine stimulation. Conversely, acquisition of IFN-γ-competence in CD4(+) T helper cells requires a differentiation process from naïve toward type 1 (Th1) cells, whi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344072
更新日期:2014-07-01 00:00:00
abstract::Accumulating evidence suggests that alloreactive memory T cells (Tm) may form a barrier to tolerance induction in large animals and humans due in part to a resistance to suppression by Treg. However, why Tm are resistant to regulation and how the Tm response to an allograft differs from that of naïve T cells, which ar...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040671
更新日期:2010-11-01 00:00:00