Abstract:
:The proteins p40 (Mr = 40 000), p32 (Mr = 32 000), p28 (Mr = 28 000), p20 (Mr = 20 000) and p10 (Mr = 10 000) are described which occur in noncovalent association with the polymorphic alpha, beta heterodimer of class II antigens. They were investigated with respect to their molecular characteristics and their mutual structural relationship. p32, the predominant species of this group corresponds to the invariant chain gamma (Ii). In contrast to the polymorphic subunits alpha and beta, proteins p40, p28, p20 and p10 migrated like gamma in electrophoretically constant positions, when class II molecules of different subregions and different alleles were assessed by two-dimensional gel electrophoresis [1st dimension, isoelectric focusing; 2nd dimension, sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis]. Analogous to gamma, they are therefore designated invariant chains. The low Mr species of this group do not arise from higher Mr forms as preparation artefacts. Short-term pulse-chase analysis and cell-free translation of sucrose gradient-fractionated mRNA in conjunction with specific immunoprecipitation rendered the possibility unlikely that individual components of this set of proteins existed in a precursor-product relationship within the cell. Comparative enzymatic fragmentation on SDS polyacrylamide gels as well as tryptic peptide map comparisons by high performance liquid chromatography revealed a high structural relatedness among all members of this group of invariant proteins.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Zecher R,Ballhausen W,Reske K,Linder D,Schlüter M,Stirm Sdoi
10.1002/eji.1830140606subject
Has Abstractpub_date
1984-06-01 00:00:00pages
511-7issue
6eissn
0014-2980issn
1521-4141journal_volume
14pub_type
杂志文章abstract::Polypeptides synthesized in a rabbit reticulocyte lysate system directed by mRNA from the T cell line LH8-105, obtained by radiation leukemia virus-induced transformation of hen egg-white lysozyme (HEL)-specific suppressor T lymphocytes, are able, when injected into mice, to specifically suppress the antibody response...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150409
更新日期:1985-04-01 00:00:00
abstract::Cognate interactions between major histocompatibility complex class II antigen (Ag)-reactive CD4+ T helper (Th) and Ag-presenting B cells induce first the activation of B cells and their subsequent differentiation into Ig-secreting cells (IgSC). The Th cell-associated homodimeric glycoprotein CD28 has been implicated ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210527
更新日期:1991-05-01 00:00:00
abstract::Pathogens have developed a plethora of strategies to undermine host immune defenses in order to guarantee their survival. For large DNA viruses, these immune evasion mechanisms frequently rely on the expression of genes acquired from host genomes. Horizontally transferred genes include members of the immunoglobulin su...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201746984
更新日期:2017-05-01 00:00:00
abstract::In recent years, factors potentially involved in pathogenesis of rheumatoid arthritis have mostly been identified by studying well-defined patient cohorts. Characterization of antibodies from sera of affected patients, family-based heritability studies, genome-wide association scans, the analysis of environmental fact...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939514
更新日期:2009-08-01 00:00:00
abstract::It is well established that therapeutic impairment of Foxp3+ Treg in mice and humans favors immune rejection of solid tumors. Less explored is the impact Foxp3 allelic variants may have on tumor incidence, progression and therapy. In this work, we tested and demonstrate that the Foxp3fgfp reporter allele, found previo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948251
更新日期:2020-03-01 00:00:00
abstract::Dopamine by itself has not up to now been reported to activate T cell function. We show here that dopamine interacts directly with dopaminergic receptors on normal human T cells and triggers beta1 integrin-mediated T cell adhesion to a major extracellular matrix component, fibronectin (FN). Such adhesion is a characte...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200112)31:12<3504::aid-immu3504>
更新日期:2001-12-01 00:00:00
abstract::The developmental stages and the role of protein tyrosine kinases (PTK) in the maturation of CD3+CD8 alpha alpha+ intraepithelial lymphocytes (IEL) have not been extensively characterized. However, comparisons of thymic and extrathymic T cell development indicate that these processes involve some distinct signaling an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270229
更新日期:1997-02-01 00:00:00
abstract::Dendritic cells (DCs) are the key APCs not only for the priming of naïve T cells, but also for the induction and maintenance of peripheral T-cell tolerance. We have recently shown that cognate interactions between Foxp3(+) Tregs and steady-state DCs are crucial to maintain the tolerogenic potential of DCs. Using DIETE...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343738
更新日期:2014-04-01 00:00:00
abstract::Age-associated thymic involution results in a diminished capacity to regenerate T cell populations, although the magnitude of this effect is unknown. In this report, thymic function was studied in aged vs. young adult mice after lethal irradiation and administration of T cell-depleted bone marrow (BM) from young mice....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199806)28:06<1886::AID-IMM
更新日期:1998-06-01 00:00:00
abstract::There is limited insight into the mechanisms involved in the counterregulation of TLR. Given the important role of TLR3/TIR domain-containing adaptor-inducing IFN-β (TRIF)-dependent signalling in innate immunity, novel insights into its modulation is of significance in the context of many physiological and pathologica...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040547
更新日期:2010-11-01 00:00:00
abstract::Macrophages can be stimulated to produce a relatively large amount of nitric oxide, which is an important component in macrophage-mediated defense mechanisms and regulation of T cell activities. It has been known that T helper (Th) cell activation requires intimate physical interaction between T helper cells and macro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250152
更新日期:1995-01-01 00:00:00
abstract::The major role of cells of the dendritic family in immunity and tolerance has been amply documented. Since their discovery in 1973, these cells have gained increasing interest from immunologists, as they are able to detect infectious agents, migrate to secondary lymphoid tissue, and prime naive T lymphocytes, thereby ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201242480
更新日期:2012-10-01 00:00:00
abstract::A recombinant Plasmodium vivax circumsporozoite (CS) protein (rPvCS-1) has been investigated as a possible malaria sporozoite vaccine candidate. Experiments were carried out to determine whether sporozoite-specific memory cells develop in Swiss Webster mice immunized with rPvCS-1. Challenge of rPvCS-1-immunized mice w...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171213
更新日期:1987-12-01 00:00:00
abstract::The early activation antigen CD69 is a member of a supergene family of type II integral membrane proteins with a C-type lectin domain. In recent reports the genes encoding the natural killer (NK) cell-related molecules of this supergene family, NKR-P1, NK1.1 and Ly-49, were shown to be clustered in a chromosomal regio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231051
更新日期:1993-10-01 00:00:00
abstract::We recently reported that differentiation of CD8(+) T cells from the naïve to the effector state involves the upregulation of glucose-dependent metabolism. Glucose deprivation or inhibition of glycolysis by 2-deoxy-D-glucose (2-DG) selectively inhibited production of IFN-gamma but not of IL-2. To determine a more glob...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838289
更新日期:2008-09-01 00:00:00
abstract::It is now recognized that Th17 cells constitute the third effector arm of Th cells, complementing the Th1 and Th2 lineages. Although secretion of IL-17 is a primary defining characteristic of Th17 cells, the expression profile of specific chemokine receptors, cell surface molecules and cytokines are also part of the T...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200939540
更新日期:2009-06-01 00:00:00
abstract::Ia. W39 is a B cell differentiation antigen whose membrane expression is controlled by the xid gene. In this report we show that, analogous to its B cell expression, Ia. W39 is also present on a subset of Ia+ macrophages, indicating heterogeneity within that cell population. The Ir gene(s) for the antigenic determinan...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120109
更新日期:1982-01-01 00:00:00
abstract::Human NK cells can be subdivided into CD56(dim) and CD56(bright) NK cells, which exhibit different phenotypical and functional characteristics. As murine NK cells lack CD56 or a distinct correlate, direct comparative studies of NK cells in mice and humans are limited. Although CD27 is currently proposed as a feasible ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940056
更新日期:2010-05-01 00:00:00
abstract::Antigen-presenting cells (APC), including dendritic cells and macrophages, produce a large amount of interferon (IFN)-gamma, a crucial cytokine for the control of infectious diseases. To elucidate the role of IFN-gamma from APC in vivo, we employed cytokine receptor common gamma subunit (gamma c) and recombination-act...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323292
更新日期:2003-10-01 00:00:00
abstract::Previous studies have demonstrated that immunization of BALB/c mice with alpha(1----3) dextran (Dex) is accompanied by a reduction in the subsequent immune response to the acetylcholine receptor (AChR), depending on the timing of the Dex administration relative to AChR challenge. Here, we report that suppression of th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200803
更新日期:1990-08-01 00:00:00
abstract::A multiparametric analysis of the resident immune populations in the parotid, submandibular and sublingual salivary glands was done in single-cell suspensions. The incidence of T and B cells and of macrophages was assessed using phenotypic markers in immunofluorescent staining and a functional assay was used to enumer...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160313
更新日期:1986-03-01 00:00:00
abstract::Dendritic cells (DC) are sentinels of the immune system, transporting antigens from the periphery to secondary lymphoid organs. This study investigates the interactions of DC with B cells for the induction of anti-viral neutralizing antibody responses. Using the vesicular stomatitis virus glycoprotein (VSV-G) as a mod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200001)30:1<185::AID-IMMU185>3.0
更新日期:2000-01-01 00:00:00
abstract::Cyclosporin A (CsA), an immunosuppressive drug which completely suppresses the humoral response to thymus-dependent antigens, does not affect the generation of T helper (Th) cells during an in vitro stimulation of murine spleen cells with sheep red blood cells. The appearance of Th cells depends on time and their deve...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210304
更新日期:1991-03-01 00:00:00
abstract::The capacity of human peripheral blood lymphocytes (PBL) to bind antigen-IgG antibody (EA(IgG)) and antigen-IgM antibody (EA(IgM)) complexes was investigated using a rosette technique with ox erythrocytes coated with rabbit IgG or IgM antibody. It was found that while EA(IgG)-rosette-forming cells (RFC) were detected ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050812
更新日期:1975-08-01 00:00:00
abstract::The F1 progeny of autoimmune NZB and normal SWR mice uniformly develop severe and accelerated lupus nephritis. The (SWR x NZB)F1 mice produce an oligoclonally expanded population of nephritogenic anti-DNA autoantibodies that share a recurrent cross-reactive idiotype (Id564), use highly homologous VH genes and surprisi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220302
更新日期:1992-03-01 00:00:00
abstract::A serological cross-reactivity between env gp120 glycoprotein of the human immunodeficiency virus (HIV) and a human cellular surface protein has been defined by a monoclonal antibody (M38) raised against HIV. The cellular antigen is a protein of ca. 80 kDa expressed on a small fraction of mononuclear cells in peripher...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171218
更新日期:1987-12-01 00:00:00
abstract::In rheumatoid arthritis (RA), T cells in the inflamed joint are considered to play a crucial role in the pathogenesis. However, despite the fact that synovial T cells have an activated memory phenotype, they are functionally suppressed upon combined CD3 and CD28 stimulation. Here, we analyzed the contribution of both ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199805)28:05<1554::AID-IMM
更新日期:1998-05-01 00:00:00
abstract::Antigen-presenting macrophages (M phi) were derived from day 7 cultures of bone marrow stem cells using L cell conditioned medium. The adherent bone marrow-derived macrophages (BMM phi) were 100% esterase-positive, 95% positive for C3 receptors, 93% positive for Fc receptors, and 95% actively phagocytic. Indirect immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121202
更新日期:1982-12-01 00:00:00
abstract::Human ILCs are classically categorized into five subsets; cytotoxic CD127- CD94+ NK cells and non-cytotoxic CD127+ CD94- , ILC1s, ILC2s, ILC3s, and LTi cells. Here, we identify a previously unrecognized subset within the CD127+ ILC population, characterized by the expression of the cytotoxic marker CD94. These CD94+ I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048696
更新日期:2020-12-09 00:00:00
abstract::The B cell line 721.174 has lost the ability to present intracellular antigens to major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTL). This phenotype results from a homozygous deletion in the MHC that includes the peptide transporter genes TAP1 and TAP2, and the proteasome subunits ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250238
更新日期:1995-02-01 00:00:00