Abstract:
:Immunodominant T cell epitopes from the autoantigen human cartilage glycoprotein 39 have previously been mapped in the context of HLA-DR*0401 and *0402, using mice expressing HLA-DR4 transgenes. We measured the dissociation rates of these epitopes from soluble recombinant DR*0401 and DR*0402 to assess the relationship between peptide/HLA-DR4 kinetic stability and immunogenicity. Experiments were performed at endosomal pH (5.5) and at cell surface pH (7), in the absence and presence of soluble recombinant HLA-DM (sDM). All (4/4) immunodominant peptide/HLA-DR complexes exhibit dissociation half-times of 1h to several days. In contrast, most (3/4) non-immunodominant complexes dissociate with half-times <30 min under at least one of these conditions. Interestingly, a complex which is stable except in the presence of HLA-DM at pH 5.5 is immunogenic only following peptide immunization, while a complex which is stable at acidic but not at neutral pH, is non-immunogenic following either whole protein or peptide immunization. These data indicate that kinetic stability of peptide/MHC complexes in vivo is a key determinant of immunogenicity.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Hall FC,Rabinowitz JD,Busch R,Visconti KC,Belmares M,Patil NS,Cope AP,Patel S,McConnell HM,Mellins ED,Sonderstrup Gdoi
10.1002/1521-4141(200203)32:3<662::AID-IMMU662>3.0keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
662-70issue
3eissn
0014-2980issn
1521-4141journal_volume
32pub_type
杂志文章abstract::To study allelic exclusion of TcR genes we analyzed two types (I and II) of TcR beta transgenic mice. T cells derived from both types of mice contained similar amounts of transgenic RNA transcripts; however, surface expression of the transgenic beta chain was drastically reduced in type II compared to type I. In type ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200227
更新日期:1990-02-01 00:00:00
abstract::A method is described for achieving the efficient production of proteins of biological interest by the establishment of hybridomas from lymphocytes of transgenic animals carrying a fusion gene having promoter-regulatory sequences functional in lymphocytes fused to the coding sequences of the protein to be produced. Wh...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160829
更新日期:1986-08-01 00:00:00
abstract::We have previously reported the development of a two-step culture system in which soluble anti-mu monoclonal antibodies prime small resting murine B cells to secrete immunoglobulin (Ig) in response to restimulation with a mixture of interleukin-4 (IL-4) and IL-5. Here we have extended these studies to investigate the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230243
更新日期:1993-02-01 00:00:00
abstract::We have investigated the vaccine potential of two peptides derived from the 6-kDa early secretory antigenic target (ESAT)-6 antigen in the mouse model of tuberculosis. The peptides were both strongly immunogenic in B6CBAF1 (H-2b,k) mice and primed recall responses of the same intensity after immunization. However, bot...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200006)30:6<1724::AID-IMMU1724>3
更新日期:2000-06-01 00:00:00
abstract::During mammalian pregnancy, one or more semiallogeneic fetuses gestate in direct contact with the maternal circulation and uterine tissue. However, a damaging maternal immune response is not normally provoked. We studied two possible mechanisms for this maternal-fetal tolerance, alone and in combination. First, we dir...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3479::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::Type I IFN play a very important role in immunity against viral infections. Murine type I IFN belongs to a multigene family including 14 IFN-alpha subtypes but the biological functions of IFN-alpha subtypes in retroviral infections are unknown. We have used the Friend retrovirus model to determine the anti-viral effec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838311
更新日期:2009-01-01 00:00:00
abstract::With the aid of a specific rabbit antibody preparation to purified monoclonal murine IgE, two plaque-forming cell (PFC) assays have been developed for the detection and enumeration of mouse IgE-secreting cells. The first assay, utilizing protein A-coated sheep red cells (protein A-SRC), detected antibody-secreting cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101210
更新日期:1980-12-01 00:00:00
abstract::A commonly held postulate regarding the etiology of rheumatoid arthritis (RA) is that of antigenic mimicry. Recent interest has focused on the mycobacterial 65-kDa heat-shock protein (hsp) as a putative causal agent. The 65-kDa hsp has over 40% sequence homology with the human hsp 60, and elevated synovial T cell resp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220529
更新日期:1992-05-01 00:00:00
abstract::Cells expressing the chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) and the chemokine C receptor (CCR)4 were consistently detected in the circulation of healthy subjects, whereas numbers of cells expressing CCR3 were much lower. While all CCR4+ cells were T cells, a small proportion of CRT...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200010)30:10<2972::AID-IMMU2972>
更新日期:2000-10-01 00:00:00
abstract::On the premise that an individual with an intact immune system has the capability to develop both cellular and antibody immune responses supported by the balance between the lymphokines secreted by T helper (Th) cells, we studied the interaction between different types of Th cell clones in vivo and the parameters that...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250312
更新日期:1995-03-01 00:00:00
abstract::The immaturity of the neonatal immune system in mice is associated with defective IFN-gamma production and Th2-biased immune responses. In this study, infants vaccinated at birth with BCG produced similar concentrations of IFN-gamma in response to PPD and showed similar frequencies of IFN-gamma-producing lymphocytes a...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/1521-4141(200105)31:5<1531::AID-IMMU1531>3
更新日期:2001-05-01 00:00:00
abstract::Alpha4-mediated signaling is involved in a variety of functions in mammalian cells. To determine whether this is true for immunocompetent cells, we generated mutant (Lck-alpha4-) mice in which the alpha4 gene was deleted in a T cell-specific manner using the Cre/loxP system. These mice showed impaired early T cell dev...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323720
更新日期:2003-07-01 00:00:00
abstract::The use of fetal bovine serum (FBS) for the culture and expansion of mesenchymal stromal cells (MSCs) limits their possible clinical applications. Although some recent studies recommended substituting FBS with human platelet lysate (HPL) for the expansion of MSCs for clinical use, the functional capacity of the expand...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141542
更新日期:2011-11-01 00:00:00
abstract::Human T cell clones expressing the T cell receptor (TcR) gamma/delta were isolated from peripheral blood lymphocytes of two unrelated donors. The TcR gamma/delta+ clones derived from one of these donors were all of the Ti gamma A+, delta-TCS1-, BB3+ phenotype indicating the exclusive use of the V gamma 9 and V delta 3...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191120
更新日期:1989-11-01 00:00:00
abstract::Mast cells are important cellular constituents of epithelial-mesenchymal interactions, densely located at sites of microbial entry into the host where they are continuously exposed to products from commensals. In order to avoid excessive activation and the associated pathology, mast cell responses to TLR agonists must...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242786
更新日期:2013-04-01 00:00:00
abstract::Even if omalizumab is broadly used in the treatment of severe, allergic asthma, the immunological effects in long-term treated patients have not been fully elucidated. To this aim, a cohort of 15 allergic asthmatic patients treated with omalizumab for at least three years was compared with 12 allergic asthma patients ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847668
更新日期:2018-12-01 00:00:00
abstract::Using a culture system which supports primary T cell proliferative responses to various antigens we have detected mouse red blood cell (RBC)-reactive T cells in lymphoid tissues from untreated mice. The release of significant amounts of interleukin 2 (IL2) indicates that T helper (or helper/inducer) cells are activate...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170610
更新日期:1987-06-01 00:00:00
abstract::The autoimmune regulator (AIRE) promotes "promiscuous" expression of tissue-restricted antigens (TRA) in thymic medullary epithelial cells to facilitate thymic deletion of autoreactive T-cells. Here, we show that AIRE-deficient mice showed an earlier development of myelin oligonucleotide glycoprotein (MOG)-induced exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040679
更新日期:2010-12-01 00:00:00
abstract::The MHC class Ib molecule H2-M3 presents N-formylated peptides from the N terminus of proteins encoded by the mitochondrial genome to CTL. A panel of CTL specific for a peptide derived from a mitochondrial protein, either COI or ND1, was used to determine the optimal peptide length for sensitizing antigen-deficient ta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3773::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::This study describes a general strategy to produce hybrid monoclonal antibodies that are capable of targeting human cytotoxic T lymphocytes (CTL) against any cell carrying the appropriate target antigen. This is done by fusing a HAT-sensitive, G418-resistant anti-T3 hybridoma with immune spleen cells (or with other hy...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170118
更新日期:1987-01-01 00:00:00
abstract::Antigen-independent adhesion of CD4+ T lymphocytes to Epstein-Barr virus (EBV)-transformed B cells is mediated by CD2/lymphocyte function-associated antigen (LFA)-3 and LFA-1/intracellular adhesion molecule (ICAM)-1. Although some anti-CD4 antibodies block the antigen-independent adhesion of CD4+ T lymphocytes, the CD...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200326
更新日期:1990-03-01 00:00:00
abstract::Mutations in TNFRSF1A encoding TNF receptor 1 (TNFR1) cause the autosomal dominant TNF receptor-associated periodic syndrome (TRAPS): a systemic autoinflammatory disorder. Misfolding, intracellular aggregation, and ligand-independent signaling by mutant TNFR1 are central to disease pathophysiology. Our aim was to unde...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344328
更新日期:2014-07-01 00:00:00
abstract::Accumulating evidence suggests that alloreactive memory T cells (Tm) may form a barrier to tolerance induction in large animals and humans due in part to a resistance to suppression by Treg. However, why Tm are resistant to regulation and how the Tm response to an allograft differs from that of naïve T cells, which ar...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040671
更新日期:2010-11-01 00:00:00
abstract::Lymphocyte infiltration to pancreatic islets is associated to chemoattraction, as are other inflammatory autoimmune processes. We examined whether development of insulitis and diabetes depends on chemoattraction of lymphocytes via the CCR5 chemokine receptor. In non-obese diabetic (NOD) mice, a substantial fraction of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324285
更新日期:2004-02-01 00:00:00
abstract::Induction of cytolytic activity of peripheral blood mononuclear cells towards target cells was studied by preparing bispecific F(ab')2 which was composed of two Fab' fragments, one of which was derived from anti-CD3 monoclonal antibody and the other from anti-tumor monoclonal antibody. After reduction of the interchan...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190814
更新日期:1989-08-01 00:00:00
abstract::Among human peripheral blood B cells we localized the precursors of two interleukin-dependent B cell activation processes: the specific response to a particulate antigen, trinitrophenylated polyacrylamide beads (TNP-PAA) and the polyclonally induced response to pokeweed mitogen. In both cases the precursors belong to ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161019
更新日期:1986-10-01 00:00:00
abstract::Interleukin-15 (IL-15) is an inflammatory cytokine whose role in autoimmune diseases has not been fully elucidated. Th17 cells have been shown to play critical roles in experimental autoimmune encephalomyelitis (EAE) models. In this study, we demonstrate that blockade of IL-15 signaling by TMβ-1 mAb treatment aggravat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444675
更新日期:2014-11-01 00:00:00
abstract::In humans and in BALB/c mice, immune responses to the hormone insulin use evolutionarily related VHV (human) and VHIX (murine) gene families. To determine if these structural relationships include regulatory elements, BALB/c mice were pretreated with autologous immunoglobulin G (IgG) monoclonal antibodies (mAb) that r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220938
更新日期:1992-09-01 00:00:00
abstract::The migration of immunocytes within the extracellular matrix (ECM) is influenced by the activation state of the incoming cell and its responses to the presence of chemokines and cytokines. We studied the regulatory role of TGF-beta1 on T cell homing to secondary lymphatic organs, such as the spleen, and chemotaxis wit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200201)32:1<193::AID-IMMU193>3.0
更新日期:2002-01-01 00:00:00
abstract::Peptides presented by MHC class I molecules are mostly derived from proteins synthesized by the antigen-presenting cell itself, while peptides presented by MHC class II molecules are predominantly from materials acquired by endocytosis. External antigens can also be presented by MHC class I molecules in a process refe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445156
更新日期:2015-02-01 00:00:00