Abstract:
:A series of rat monoclonal antibodies (mAb) directed against the Thy-1 molecule have been evaluated for their ability to stimulate immature (CD4-8-) murine thymocytes. CD4-8- thymocytes could be induced to proliferate by several anti-Thy-1 mAb provided that exogenous interleukin 1 or interleukin 2 was provided. Apparently non-stimulatory anti-Thy-1 mAb could be rendered stimulatory if cross-linked by a rabbit anti-rat immunoglobulin reagent. Anti-Thy-1-stimulated CD4-8- thymocytes were found to uniformly express CD3 but did not stain positively with F23.1, a mAb reactive with alpha/beta T cell receptors utilizing the V beta 8 gene family. Immunoprecipitation analysis of such stimulated CD4-8- thymocytes indicated that they expressed a CD3-associated heterodimer of 45 kDa/35 kDa most likely corresponding to the product of the gamma and (putative) delta T cell receptor genes. Taken together, these data demonstrate that anti-Thy-1 mAb selectively activate a subset of CD4-8- thymocytes committed to the gamma/delta T cell receptor lineage.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
MacDonald HR,Miescher GC,Howe RCdoi
10.1002/eji.1830171127subject
Has Abstractpub_date
1987-11-01 00:00:00pages
1677-80issue
11eissn
0014-2980issn
1521-4141journal_volume
17pub_type
杂志文章abstract::By immunizing Lewis rats with myelin basic protein (MBP) peptide variants derived from the major encephalitogenic epitope of guinea pig (MBP(68-88) and then isolating encephalitogenic T cells from these animals, we demonstrated that the variant peptides do not elicit the same encephalitogenic T cell subsets as those i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250631
更新日期:1995-06-01 00:00:00
abstract::Indoleamine 2,3-dioxygenase (IDO) suppresses T cell responses by its action in catabolising tryptophan. It is important in maintenance of immune privilege in the placenta. We investigated the activity of IDO in the cornea, following corneal transplantation and the effect of IDO over-expression in donor corneal endothe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535238
更新日期:2006-03-01 00:00:00
abstract::The genetic control of in vitro primary syngeneic sensitization on monolayers of thyroid epithelial cells was investigated both at the level of the responder lymphocytes and that of stimulator thyroid epithelial cells using congenic resistant strains of mice. It clearly appears that similar to the in vivo model of exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131115
更新日期:1983-11-01 00:00:00
abstract::The desire to induce HIV-1-specific responses soon after birth to prevent breast milk transmission of HIV-1 led us to propose a vaccine regimen which primes HIV-1-specific T cells using a recombinant Mycobacterium bovis bacillus Calmette-Guérin (rBCG) vaccine. Because attenuated live bacterial vaccines are typically n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141962
更新日期:2011-12-01 00:00:00
abstract::MyD88 is a key adaptor molecule in innate resistance, engaged in most Toll-like receptor, as well as IL-1 and IL-18, signalling. Here, we analyzed the role of MyD88 in innate resistance during infection with vesicular stomatitis virus (VSV) using myd88(-/-) mice. We found an increased susceptibility to VSV in myd88(-/...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737310
更新日期:2007-09-01 00:00:00
abstract::Previous studies have shown that the fecal flora of patients with Crohn's disease (CD) differed from the flora of healthy subjects by a higher number of anaerobic gram-positive coccoid rods. Sera from patients with CD agglutinated four strains of coccoid rods (Me44, C18, Me46 and Me47) more frequently and stronger tha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150823
更新日期:1985-08-01 00:00:00
abstract::A cDNA encoding the human beta Fc gamma receptor II (FcRII) was isolated from a placental cDNA library. Analysis of the predicted amino acid sequence indicates that this receptor is synthesized with a 42-amino acid leader sequence. The mature protein consists of 249 amino acids. The leader sequence and the cytoplasmic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200624
更新日期:1990-06-01 00:00:00
abstract::Surfactant protein A (SP-A) and surfactant protein D (SP-D) are collectins, and both proteins were shown to interact with influenza A virus and alveolar macrophages. However, it is not known whether SP-A and SP-D can serve as opsonins for the phagocytosis of influenza A virus by alveolar macrophages. In the present st...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270413
更新日期:1997-04-01 00:00:00
abstract::CBA/N mice have an X-linked immune defect in B lymphocyte function which leads to their inability to respond to several thymus-independent antigens. We report here that these mice and immunologically defective F1 male (CBA/N X DBA/2N) mice can respond to Brucella abortus and to 2,4,6-trinitrophenyl derivatives of Bruc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830080703
更新日期:1978-07-01 00:00:00
abstract::The T cell mitogens, concanavalin A and the monoclonal antibody OKT3, cause a rapid activation of ornithine decarboxylase (ODC) activity in human T lymphocytes, maximal within 10 min of mitogen addition. Nonmitogenic ligands to T cell surface structures do not induce ODC. The enzyme induction is dependent upon an inta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150808
更新日期:1985-08-01 00:00:00
abstract::Similarities between the physical structures of avian and mammalian major histocompatibility complex (MHC) class I glycoproteins have been proposed based on comparative alignment of their amino acid sequences. To investigate the physical structure of the chicken class I glycoprotein, we cloned the cDNA representing th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250740
更新日期:1995-07-01 00:00:00
abstract::The initiation of CD8(+) T cell (CTL) immune responses can occur via cross-priming. Recent data suggested a relationship between cross-presentation and immunodominance of epitope-specific T cells. To test this association, we evaluated the efficacy of cross-presentation for several virus epitopes in vitro and examined...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939973
更新日期:2010-08-01 00:00:00
abstract::Cytomegalovirus latently infects myeloid cells; however, the acute effects of the virus on this cell subset are poorly characterised. We demonstrate that systemic cytomegalovirus infection induced rapid activation of monocytes in the bone marrow, characterised by upregulation of CD69, CD11c, Ly6C and M-CSF receptor. A...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201243109
更新日期:2014-02-01 00:00:00
abstract::Low zone tolerance (LZT) to contact allergens is induced by epicutaneous exposure to haptens in subsensitizing doses resulting in an inhibition of contact hypersensitivity (CHS), which, in contrast, occurs after sensitization with immunogenic doses of allergens. Performing the protocol of tolerance induction resulted ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425402
更新日期:2004-11-01 00:00:00
abstract::Strains CBA (M523) (= M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B 10.D2 (M504 (= M504) and b 10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CB...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090208
更新日期:1979-02-01 00:00:00
abstract::The murine T cell response to heterologous insulins provides a good model system for studying the mechanism of immune response (Ir)-gene function, since insulin is a small, chemically well-defined molecule. H-2b mice respond predominantly to A chain loop determinants of beef insulin, presented by the I-A epitope Ia. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830141208
更新日期:1984-12-01 00:00:00
abstract::The immune mechanisms underlying immunization-induced protection of mice from lethal central nervous system (CNS) listeriosis were evaluated by immunohistochemistry, flow cytometry of leukocytes isolated from the brain, reverse transcription-polymerase chain reaction analysis of intracerebral (i.c.) tumor-necrosis fac...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250839
更新日期:1995-08-01 00:00:00
abstract::The approximately 20-kDa heat-labile toxin produced by enterotoxigenic Bacteroides fragilis is known to be associated with the development of enteritis. However, the molecular mechanism involved is not yet fully understood. In this study, we assessed whether B. fragilis enterotoxin (BFT)-induced enteritis is related t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526321
更新日期:2005-09-01 00:00:00
abstract::Th2 lymphocytes deliver essential signals for induction of asthmatic airway inflammation. We previously found that airway antigen challenge induces recruitment of Gr-1(+) neutrophils prior to the recruitment of Th2 cells. We examined, therefore, whether Gr-1(+) cells contribute to the development of Th2-dependent airw...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838985
更新日期:2009-08-01 00:00:00
abstract::Allergic asthma is a chronic inflammation of the airways mediated by an adaptive type 2 immune response. Upon allergen exposure, group 2 innate lymphoid cells (ILC2s) can be rapidly activated and represent an early innate source of IL-5 and IL-13. Here, we used a house dust mite (HDM)-driven asthma mouse model to stud...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546119
更新日期:2016-06-01 00:00:00
abstract::Infection with parasitic worms (helminths) alters host immune responses and can inhibit pathogenic inflammation. Helminth infection promotes a strong Th2 and T regulatory response while suppressing Th1 and Th17 function. Th2 responses are largely dependent on transcriptional programs directed by Stat6-signaling. We ex...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848072
更新日期:2020-10-16 00:00:00
abstract::Natural antibodies (Ab) reacting with self antigens have been shown to be present in all individuals. These autoantibodies (auto-Ab) can be either pathogenic or non-pathogenic. Auto-Ab reacting with the alpha-subunit of the high-affinity receptor for IgE (FcepsilonRIalpha) have been implicated in the pathogenesis of a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199904)29:04<1149::AID-IMM
更新日期:1999-04-01 00:00:00
abstract::To identify environmental stimuli that induce dendritic cell (DC) maturation, we exposed human monocyte-derived immature DC to apoptotic or necrotic cells and measured the levels of expression of costimulatory molecules and cytokine production. While most necrotic or apoptotic cells did not have any effect, some induc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200002)30:2<705::AID-IMMU705>3.0
更新日期:2000-02-01 00:00:00
abstract::Dendritic cells (DC) play the dual task of initiating cellular immunity against potentially harmful foreign antigens (Ag), while maintaining immunological tolerance to self-Ag and environmental Ag. As an approach to induce Ag-specific suppression, we and others introduced CD95 ligand (L) cDNA into DC. The resulting "k...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<1035::AID-IMMU1035>3
更新日期:2002-04-01 00:00:00
abstract::The expression of an X-linked defect in the CBA/N strain of mice has been found to result in a number of immune abnormalities. These include low responsiveness to antigens and a greatly reduced ability to respond to many of the common B cell mitogens. An in vitro manifestation of this condition is the virtual inabilit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220419
更新日期:1992-04-01 00:00:00
abstract::The early activation antigen CD69 is a member of a supergene family of type II integral membrane proteins with a C-type lectin domain. In recent reports the genes encoding the natural killer (NK) cell-related molecules of this supergene family, NKR-P1, NK1.1 and Ly-49, were shown to be clustered in a chromosomal regio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231051
更新日期:1993-10-01 00:00:00
abstract::The death of individual cells is a frequent and physiological event in the mammalian immune system and most often occurs by apoptosis. It is becoming increasingly clear that cell death is also induced during bacterial infections. Here we report that, in addition to the apoptotic form already established, a necrosis-li...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324582
更新日期:2004-05-01 00:00:00
abstract::Hypersensitivity to nickel (Ni) represents the most common manifestation of contact allergy in humans. The role of metal-specific T cells in this disease is well established, but the molecular interactions involved in their activation are poorly understood. We examined the T cell receptor (TCR) repertoire in T cells a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270808
更新日期:1997-08-01 00:00:00
abstract::IL-5 preferentially activates B1 cells to produce natural antibodies cross-reactive to self antigens. To determine the role of IL-5 in antibody-mediated autoimmune disease, we generated systemic lupus erythematosus (SLE)-prone (NZB x NZW)F1 mice congenic for IL-5 transgene (TG-F1). The transgene unexpectedly reduced t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425267
更新日期:2004-10-01 00:00:00
abstract::Treatment of human peripheral blood lymphocytes with cloned N alpha-desacetylthymosin alpha 1 induced interferon production. The kinetics is similar to that of mitogen-induced interferon induction. N alpha-desacetylthymosin alpha 1, in combination with mitogen, augments the amount of interferon produced. This interfer...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120314
更新日期:1982-03-01 00:00:00