Abstract:
:Similarities between the physical structures of avian and mammalian major histocompatibility complex (MHC) class I glycoproteins have been proposed based on comparative alignment of their amino acid sequences. To investigate the physical structure of the chicken class I glycoprotein, we cloned the cDNA representing the BFIV locus of the B21 haplotype. A unique, chimeric class I glycoprotein was constructed by incorporating an epitope tag (FLAG) at the N terminus. Monoclonal antibodies to the FLAG epitope served to monitor cell-surface expression for functional analysis of the BFIV21 class I glycoprotein. The chimeric class I glycoprotein was expressed in target cells using an avian leukosis virus (ALV)-derived retrovirus vector (RCASBP). The presence of the FLAG epitope did not interfere with either alloantibody recognition or cytotoxic T lymphocyte interaction. Functional analysis employing site-directed mutagenesis identified BF amino acid residues forming serologic epitopes as well as residues important in antigen presentation to ALV-induced cytotoxic T lymphocytes. BF residues 78 and 81, corresponding to HLA 79 and 82, form an antibody epitope with a slight effect on ALV antigen presentation, consistent with their predicted orientation based on the HLA-A2 crystal structure. Alignment of the BFIV21 sequence with previously published BFIV sequences revealed polymorphisms at position 34 (HLA 34), a monomorphic residues in HLA and H-2. Residue 34 is located in pocket B and is predicted to contact the main-chain carbon of peptides bound in HLA-A2. A site-directed substitution in BFIV residue 34 dramatically alters ALV antigen presentation by the BFIV21 class I glycoprotein. These data indicate that the physical molecular structure of the chicken MHC class I glycoprotein is similar to HLA.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Fulton JE,Thacker EL,Bacon LD,Hunt HDdoi
10.1002/eji.1830250740subject
Has Abstractpub_date
1995-07-01 00:00:00pages
2069-76issue
7eissn
0014-2980issn
1521-4141journal_volume
25pub_type
杂志文章abstract::We studied the competitive repopulation by different B cells of irradiated mice reconstituted with bone marrow from either congenic or Ig-transgenic (TG) mice mixed at different ratios. We found that after reconstitution, the number of B cells recovered in the different chimeras is similar and independent of the ratio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250636
更新日期:1995-06-01 00:00:00
abstract::Peptides presented by MHC class I molecules are mostly derived from proteins synthesized by the antigen-presenting cell itself, while peptides presented by MHC class II molecules are predominantly from materials acquired by endocytosis. External antigens can also be presented by MHC class I molecules in a process refe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445156
更新日期:2015-02-01 00:00:00
abstract::The immunocytochemical characteristics of large granular lymphocytes (LGL), isolated from the liver sinusoids of euthymic and athymic (nude) rats, were investigated in electron microscopy by the immunoperoxidase technique. The LGL were found positive for MRC OX-8 (natural killer cells and cytotoxic/suppressor T cells)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171006
更新日期:1987-10-01 00:00:00
abstract::Antigen-presenting macrophages (M phi) were derived from day 7 cultures of bone marrow stem cells using L cell conditioned medium. The adherent bone marrow-derived macrophages (BMM phi) were 100% esterase-positive, 95% positive for C3 receptors, 93% positive for Fc receptors, and 95% actively phagocytic. Indirect immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121202
更新日期:1982-12-01 00:00:00
abstract::Polyclonal differentiation of unprimed B cells into IgM-producing cells induced by lipopolysaccharide (LPS) or T cell-derived lymphokine B151-TRF2 has been shown to contain a process of I-A-restricted B-B cell interaction, so that the B cell responses are inhibited by monoclonal antibodies (mAb) specific for I-A molec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191208
更新日期:1989-12-01 00:00:00
abstract::A hallmark of parasite infection is the accumulation of innate immune cells, notably granulocytes and mast cells, at the site of infection. While this is typically viewed as a transient response, with the tissue returning to steady state once the infection is cleared, we found that mast cells accumulated in the large-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646738
更新日期:2017-02-01 00:00:00
abstract::Rapid IgE desensitization provides temporary tolerization for patients who have presented severe hypersensitivity reactions to food and drugs, protecting them from anaphylaxis, but the underlying mechanisms are still incompletely understood. Thus, here we develop an effective and reproducible in vitro model of rapid I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040810
更新日期:2011-04-01 00:00:00
abstract::The identification of soluble factors involved in stem cell renewal is a major goal in the assessment of the BM niche. We have previously shown that human endothelial cell (EC) supernatants can induce the proliferation of hematopoietic progenitor cells (HPCs), especially after stimulation with IL-1β. To identify new p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040986
更新日期:2011-06-01 00:00:00
abstract::An expanding spectrum of acute and chronic non-infectious inflammatory diseases is uniquely responsive to IL-1β neutralization. IL-1β-mediated diseases are often called "auto-inflammatory" and the dominant finding is the release of the active form of IL-1β driven by endogenous molecules acting on the monocyte/macropha...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201141550
更新日期:2011-05-01 00:00:00
abstract::A murine monoclonal IgM antibody recognizes an antigen (Mo3e) found on the surface of human peripheral blood monocytes stimulated with phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide and muramyl dipeptide and blocks the monocyte response to migration inhibitory factor (MIF). We utilized Western blot and immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190423
更新日期:1989-04-01 00:00:00
abstract::Major histocompatibility complex (MHC) class II molecules have been implicated in cell adhesion in two ways. In addition to the well-established role of class II antigens in low-affinity adhesion provided by interactions between class II and CD4, recent data indicated that class II may also induce adhesion between T a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210921
更新日期:1991-09-01 00:00:00
abstract::Somatic hypermutation in B cells undergoing T cell dependent immune responses generates high affinity antibodies that provide protective immunity. B cells also switch from the expression of immunoglobulin (Ig) M and IgD to that of other Ig classes through somatic DNA recombination. Recent work has implicated DNA stran...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200202)32:2<316::AID-IMMU316>3.0
更新日期:2002-02-01 00:00:00
abstract::IL-22 is an IFN-IL-10 cytokine family member, which is produced by activated Th1 and NK cells and acts primarily on epithelial cells. Here we demonstrate that IL-22, in contrast to its relative IFN-gamma, regulates the expression of only a few genes in keratinocytes. This is due to varied signal transduction. Gene exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535503
更新日期:2006-05-01 00:00:00
abstract::Interferon regulatory factor-1 (IRF-1) is a transcription factor regulating the expression of several cytokines. Using IRF-1 knockout (KO) mice, the role of IRF-1 in the production and activity of IL-18 was evaluated. Administration of IL-12 or concanavalin A significantly increased levels of circulating IL-18 in wild...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200102)31:2<369::aid-immu369
更新日期:2001-02-01 00:00:00
abstract::Atopic dermatitis (AD) or eczema is the most common chronic inflammatory skin disease. It is a multifactorial disease with local and systemic immune changes. Current therapies focus on restoring the local skin barrier or inhibiting immune responses. In this issue of the European Journal of Immunology, Sehra et al. [Eu...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201646708
更新日期:2016-11-01 00:00:00
abstract::Follicular helper T (Tfh) cells provide crucial help to germinal center B (GCB) cells for proper antibody production, and a specialized subset of regulatory T cells, follicular regulatory T (Tfr) cells, modulate this process. However, Tfr-cell function in the GC is not well understood. Here, we define Tfr cells as a C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546094
更新日期:2016-05-01 00:00:00
abstract::Exosomes from APC are nano-vesicles that can induce antigen-specific T cell responses and are presently explored as therapeutic tools in different clinical settings. Investigations of the capacity of exosomes to stimulate T cells in vitro have mostly been performed on T cell hybridomas, clones or lines. Whether exosom...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535615
更新日期:2006-07-01 00:00:00
abstract::Dendritic cells isolated from sheep afferent lymph were examined for their ability to bind soluble protein and peptide antigens labeled with fluorescein both in in vitro assays and following intradermal injection of antigen in vivo. Analysis of dendritic cells by flow cytometry revealed weak direct binding of proteins...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201102
更新日期:1990-11-01 00:00:00
abstract::MRL/MP-lpr/lpr mice are homozygous for the lpr mutation that results in the accumulation of phenotypically abnormal cells (CD3+CD4+CD8-) in all lymphoid issues. Although no major abnormalities in the T cell receptor repertoire expressed by such lpr cells have been reported, the lpr mutation is a major disease-accelera...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220432
更新日期:1992-04-01 00:00:00
abstract::We studied the capacity of human factor H to promote the secretion of a lymphocyte-activating factor (LAF) by human monocytes cultured under serum-free conditions. Presence of LAF in the culture supernatants was assessed with the mouse thymocyte assay. Highly purified factor H alone had no effect on thymocyte prolifer...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210416
更新日期:1991-04-01 00:00:00
abstract::HSP gp96-based vaccines have been trialled in rodent models and, more recently, in humans. Better understanding of gp96's immunomodulatory role will help with the design of more effective strategies for treatment of cancer and infectious diseases. In this study, we monitored the activities of T cells and activation of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939593
更新日期:2009-11-01 00:00:00
abstract::Mutations in TNFRSF1A encoding TNF receptor 1 (TNFR1) cause the autosomal dominant TNF receptor-associated periodic syndrome (TRAPS): a systemic autoinflammatory disorder. Misfolding, intracellular aggregation, and ligand-independent signaling by mutant TNFR1 are central to disease pathophysiology. Our aim was to unde...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344328
更新日期:2014-07-01 00:00:00
abstract::Twelve continuous rat tissue cultured mast cell (MC) lines were established by prolonged culture of rat peritoneal MC in the absence of added factors or feeder layers. Two of these lines, RCMC1 and RCMC2, have been briefly described previously, seven others are now also described. Both RCMC1 and RCMC2 lack a marker ch...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200127
更新日期:1990-01-01 00:00:00
abstract::Studies based on either MHC class II-knockout or CD4+ T cell-depleted murine models have demonstrated a critical role for CD4+ T cells in the generation of CD8+ T cell memory. However, it is difficult to extend these findings to immunocompromised humans where a complete loss of CD4+ T cells is rarely observed. Here, w...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737933
更新日期:2008-07-01 00:00:00
abstract::Soluble HLA class I (sHLA-I) molecules can regulate survival of NK cells and their anti-tumor killing activity. Herein, we have analysed whether interaction of sHLA-I with CD8 and/or different isoforms of killer Ig-like receptors (KIR) induced secretion of transforming growth factor (TGF)-beta1. CD8+KIR- NK cell clone...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939728
更新日期:2009-12-01 00:00:00
abstract::It has been reported that Thy-1+CD3+CD4-CD8- cells as well as Langerhans cells migrate from organ-cultured murine skin into culture medium. We examined whether these Thy-1+ populations of migrating cells were derived from Thy-1+ dendritic epidermal T cells (Thy-1+ DEC) and found that they were Thy-1+CD3+CD4-CD8-gamma/...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230746
更新日期:1993-07-01 00:00:00
abstract::We have constructed four different deletion mutants of a chimeric mouse-human IgG3 anti-(4-hydroxy-3-nitrophenyl)acetyl/(5-iodo-4 hydroxy-3 nitrophenyl) acetyl (NP/NIP) antibody lacking one or more of the four exons coding for the hinge region. The mutant variants all retained intact hinge region epitopes since they a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190912
更新日期:1989-09-01 00:00:00
abstract::The effect of anti-Ia antibody on the development of murine neonatal self-Ia-reactive T lymphocytes in the thymus was studied. A C57BL/6 (B6) mouse pregnant with (B6 X C3H/He)F1 (B6C3F1) embryos was injected with monoclonal anti-I-Ek (m alpha I-Ek) antibody every other day starting from the 12th day of gestation. The ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160719
更新日期:1986-07-01 00:00:00
abstract::The interaction between T cell receptors (TCR) and peptide-major histocompatibility complex (pMHC) antigens can lead to varying degrees of agonism (T cell activation), or antagonism. The P14 TCR recognises the lymphocytic choriomeningitis virus (LCMV)-derived peptide, gp33 residues 33-41 (KAVYNFATC), presented in the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636743
更新日期:2007-03-01 00:00:00
abstract::The contribution of Gal alpha 1,3Gal (alpha-Gal) to cell-mediated organ xenograft rejection is controversial. We have used recombinant lentiviruses encoding a porcine alpha 1,3 galactosyltransferase (alpha 1,3GalT) to obtain alpha-Gal-expressing primary human aortic endothelial cells (HAEC) at a frequency of 70-90%. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324683
更新日期:2004-04-01 00:00:00