Abstract:
:The contribution of Gal alpha 1,3Gal (alpha-Gal) to cell-mediated organ xenograft rejection is controversial. We have used recombinant lentiviruses encoding a porcine alpha 1,3 galactosyltransferase (alpha 1,3GalT) to obtain alpha-Gal-expressing primary human aortic endothelial cells (HAEC) at a frequency of 70-90%. These cells were compared to non-transduced and mock-transduced HAEC with regard to their susceptibility to human NK cell-mediated lysis, ability to stimulate IFN-gamma production by NK cells, and support of NK cell adhesion under static and dynamic conditions. Using green fluorescent protein (GFP) as a reporter gene, it was shown that the frequency of green fluorescent HAEC increased until day 5 post-transduction, and at a multiplicity of infection of 2.5, it reached 98%. Lentiviral transduction did not result in activation of HAEC, and transduced HAEC responded as expected to TNF-alpha and IFN-gamma stimulation. No differences were detected between non-alpha-Gal- and alpha-Gal-expressing HAEC in terms of their susceptibility to NK cell-mediated lysis, ability to stimulate IFN-gamma production by NK cells, or ability to support NK cell adhesion under static and dynamic conditions. In conclusion, these data argue against an important role for the alpha-Gal epitope in the direct interaction between endothelium and NK cells and prove that recombinant lentiviruses are efficient gene carriers for primary human endothelial cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
He Z,Ehrnfelt C,Kumagai-Braesch M,Islam KB,Holgersson Jdoi
10.1002/eji.200324683keywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1185-95issue
4eissn
0014-2980issn
1521-4141journal_volume
34pub_type
杂志文章abstract::Several groups have described that a low percentage of in vitro cultured T cell receptor (TcR) gamma/delta cells express CD8. Contrary to TcR alpha/beta cells, however, CD8 on these TcR gamma/delta cells was shown to be a CD8 alpha homodimer. We describe here that addition of interleukin-7 (IL-7) to a short-term in vi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220902
更新日期:1992-09-01 00:00:00
abstract::Disseminated infections by the opportunistic yeast Cryptococcus neoformans are characterized by accumulation in tissues of glucuronoxylomannan (GXM), the major component of the capsular polysaccharide. We investigated binding, uptake, and disposal of GXM by peripheral blood neutrophils and monocytes, and the effect of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323388
更新日期:2003-04-01 00:00:00
abstract::Upon transplantation of T cells from a CD28 superagonist (CD28-SA) treated donor into an irradiated allogeneic host, the CD28-SA-induced activation and expansion of Treg cells inhibits acute graft versus host disease (aGvHD), while not abrogating the desired graft versus tumor effect. Human peripheral blood CD4(+) T c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445317
更新日期:2015-07-01 00:00:00
abstract::Anti-CD3 mAb can modulate graft rejection and attenuate autoimmune diseases but their mechanism(s) of action remain unclear. CD8(+) T cells with regulatory function are induced in vitro by Teplizumab, a humanized anti-CD3 antibody and inhibit responses of autologous and allogeneic T cells. They inhibit CD4(+) T-cell p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040485
更新日期:2010-10-01 00:00:00
abstract::We investigated here the potential role of Toll-like receptors (TLR) and the adaptor protein MyD88 in innate immunity responses to Cryptococcus neoformans, a pathogenic encapsulated yeast. Peritoneal macrophages from MyD88(-/-) or TLR2(-/-) mice released significantly less TNF-alpha, compared with wild-type controls, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425799
更新日期:2005-03-01 00:00:00
abstract::TGF-beta1 was examined for the ability to regulate Ag-presentation by B cells, using A20-HL B lymphoma cells bearing TNP-specific IgM receptors. Treatment of A20-HL cells with TGF-beta1 at 1 ng/ml, a concentration that inhibited proliferation, enhanced presentation of Ag internalized via surface IgM (sIgM), but not vi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324018
更新日期:2003-07-01 00:00:00
abstract::The modulatory activity of dextran sulfate with a relative molecular mass of 8 and 500 kDa and pentosan polysulfate with a relative molecular mass of 6 kDa on human T cell surface molecules CD2, CD3, CD4, CD8 and HLA-DR was investigated by analytical flow cytometry. The 500-kDa dextran sulfate induces a complete disap...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190630
更新日期:1989-06-01 00:00:00
abstract::Targeted deletion of the heterotrimeric G protein, Galphai2, in mice induces lethal colitis closely resembling ulcerative colitis. In chronic colitis, migration of circulating leukocytes into the intestinal mucosa is partially dependent on alpha4 integrins. In previous studies, short-term administration of anti-alpha4...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526022
更新日期:2005-08-01 00:00:00
abstract::Ligand binding to beta1-integrins exerts multiple effects on cells of the immune system including adhesion, spreading, haptotaxis and costimulation of T cells activated by anti-CD3. Here we show that a high-affinity ligand for beta1-integrins, the invasin (Inv) protein of Yersinia pseudotuberculosis, can induce cell d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<1129::AID-IMMU1129>3
更新日期:2002-04-01 00:00:00
abstract::Human Vgamma9Vdelta2 T cells contribute to immunity against intracellular pathogens and recognize nonpeptidic antigens, such as the mycobacterial phosphoantigen TUBAg. HIV infection is associated with a polyclonal decrease of peripheral Vgamma9Vdelta2 T cells and we previously reported that the remaining cells show a ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199901)29:01<90::AID-IMMU9
更新日期:1999-01-01 00:00:00
abstract::Previously, we have shown that the intravenous (i.v.) injection of allogeneic lymphocytes across an H-2 class I-mutant disparity leads to specific skin allograft tolerance caused by irradiation-sensitive donor T cells, which function as veto cells. In the present study, we show that the i.v. injection of H-2 class II-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200232
更新日期:1990-02-01 00:00:00
abstract::Glycosylphosphatidylinositol-anchored (GPI)-Db molecules are defective in mediating cytotoxic T lymphocytes (CTL) lysis of transfected lymphoma cells, compared to their transmembrane (TM) counterpart. This defect is manifest when antigenic peptide must be processed and presented through the endogenous pathway. These s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260938
更新日期:1996-09-01 00:00:00
abstract::Activation of murine spleen cells in vitro with soluble anti-CD3 monoclonal antibody and phorbol 12-myristate 13-acetate (PMA) induced an initial production of interleukin 2 (IL2), interferon-gamma (IFN-gamma), IL4 and IL5, followed by a refractory state during which the T cells did not produce lymphokines when stimul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210816
更新日期:1991-08-01 00:00:00
abstract::An intracellular staining procedure for the cytoskeletal marker, glial fibrillary acidic protein of astrocytes, has been developed which allows flow cytometric phenotyping of astrocytes within complex mixtures of glial cells. Employing this technique, we show here that measles virus infection of rat mixed glial cell c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220126
更新日期:1992-01-01 00:00:00
abstract::X-linked agammaglobulinemia (XLA) is a primary immunodeficiency of the B-cell compartment caused by a defective gene encoding for the tyrosine kinase (btk) essential for B cell differentiation. Affected males undergo recurrent pyogenic infections and deficient immunoglobulin production. Peripheral blood T cells from 6...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200106)31:6<1927::aid-immu1927>3
更新日期:2001-06-01 00:00:00
abstract::It is widely accepted that females have superior immune responses than males, but the ways by which sex hormones may enhance T cell responses are still poorly understood. In the present study, we analyzed the effect of estrogens on CD4 T cell activation and differentiation after immunization with exogenous antigens. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/immu.200310027
更新日期:2003-02-01 00:00:00
abstract::Interleukin-31 (IL-31) is a Th2 cell-derived cytokine that has been closely linked to pruritic skin inflammation. More recently, enhanced IL-31 serum levels have also been observed in patients with allergic rhinitis and allergic asthma. Therefore, the main aim of this study was to unravel the contribution of IL-31 to ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048547
更新日期:2020-07-10 00:00:00
abstract::Polyclonal differentiation of unprimed B cells into IgM-producing cells induced by lipopolysaccharide (LPS) or T cell-derived lymphokine B151-TRF2 has been shown to contain a process of I-A-restricted B-B cell interaction, so that the B cell responses are inhibited by monoclonal antibodies (mAb) specific for I-A molec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191208
更新日期:1989-12-01 00:00:00
abstract::Natural killer (NK) cell activity can be stimulated by different surface receptors. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating human NK cell cytotoxicity and cytokine production. Here we show that stimulation of human NK cells by anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636146
更新日期:2006-12-01 00:00:00
abstract::ICAM-1 is a cell surface glycoprotein which is one of the ligands for the leukocyte function-associated antigen (LFA-1). It is involved in leukocyte adhesion to endothelial cells as well as in immune functions requiring cell-cell contact. The quantitative expression of ICAM-1 in various cell types can be either induce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201210
更新日期:1990-12-01 00:00:00
abstract::The third component of complement C3 has been implied in the stimulation of B lymphocytes to proliferation and maturation for Ig secretion. We have reinvestigated the extent of this activation with either activated or resting murine splenic lymphocytes in serum-substituted cultures. Human C3 was used in either soluble...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150214
更新日期:1985-02-01 00:00:00
abstract::IL-12 is a potent inducer of IFN-gamma production and drives the development of Th1 cells. Human polarized Th2 cells do not express the signaling beta2-subunit of the IL-12R and, therefore, do not signal in response to IL-12. The question was raised as to what extent the loss of the IL-12Rbeta2 chain in Th2 cells has ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200104)31:4<1055::aid-immu1055>3
更新日期:2001-04-01 00:00:00
abstract::The BCR plays a central role in B cell development, survival, activation, and differentiation. We have identified the B cell novel protein 1 (BCNP1) as a new regulator of BCR signaling. BCNP1 contains a pleckstrin homology domain, three proline-rich motifs, and a potential SH2 binding site, and is predominantly expres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847985
更新日期:2019-06-01 00:00:00
abstract::We have previously demonstrated that mycobacterial lipoproteins engage TLR2 on human CD4(+) T cells and upregulate TCR-triggered IFN-γ secretion and cell proliferation in vitro. Here we examined the role of CD4(+) T-cell-expressed TLR2 in Mycobacterium tuberculosis (MTB) Ag-specific T-cell priming and in protection ag...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344100
更新日期:2014-05-01 00:00:00
abstract::A number of applications of antibodies in diagnosis and therapy require multivalent reagents either because of the polymeric nature of the antigens or because biological activity depends on an effect on the formation of homodimeric species. Here, we report a procedure for mass screening of phage-derived monomeric anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270307
更新日期:1997-03-01 00:00:00
abstract::Germinal centers support the differentiation of memory B cells and long-lived antibody-secreting cells during infection or upon vaccination. Here, we constructed mice with T cells that selectively lack the chemokine receptor CXCR5 to determine if expression of this receptor by T cells is mandatory for germinal center ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636486
更新日期:2007-01-01 00:00:00
abstract::Antioxidant systems maintain cellular redox homeostasis. The thioredoxin-1 (Trx1) and the glutathione (GSH)/glutaredoxin-1 (Grx1) systems are key players in preserving cytosolic redox balance. In fact, T lymphocytes critically rely on reducing equivalents from the Trx1 system for DNA biosynthesis during metabolic repr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848044
更新日期:2019-05-01 00:00:00
abstract::A single T cell clone can be activated by many different peptides in the context of a particular HLA molecule. To quantify the number of peptides that can be recognized by a CD4(+) T cell clone, we screened a one-bead-one-peptide synthetic peptide library and a protein database for peptides that stimulate an HLA-DR3-r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199908)29:08<2385::AID-IMM
更新日期:1999-08-01 00:00:00
abstract::Longitudinal studies on the kinetics of viral antigen specific CD8 T cell responses have led to a model whereby a relatively small subset of the primary effector CD8 T cells expanding after the first week of acute viral infection initiate a program of cell survival and differentiation into long lived memory T cells. T...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200535516
更新日期:2005-11-01 00:00:00
abstract::DNase II in macrophages cleaves the DNA of engulfed apoptotic cells and of nuclei expelled from erythroid precursor cells. Macrophages in DNase II-deficient mice accumulate undigested DNA and constitutively produce IFN-beta as well as TNF-alpha. The IFN-beta causes severe anemia in the DNase II(-/-) embryos, which die...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838559
更新日期:2008-11-01 00:00:00