Abstract:
:We investigated here the potential role of Toll-like receptors (TLR) and the adaptor protein MyD88 in innate immunity responses to Cryptococcus neoformans, a pathogenic encapsulated yeast. Peritoneal macrophages from MyD88(-/-) or TLR2(-/-) mice released significantly less TNF-alpha, compared with wild-type controls, after in vitro stimulation with whole yeasts. In contrast, no differences in TNF-alpha release were noted between macrophages from C3H/HeJ mice, which have a loss of function mutation in TLR4, relative to C3H/HeN controls. When MyD88- or TLR2-deficient mice were infected with low doses of the H99 serotype A strain, all of the control animals, but none of MyD88(-/-) and only 38% of the TLR2(-/-) animals survived, in association with higher fungal burden in the mutant mice. Both MyD88(-/-) and TLR2(-/-) animals showed decreased TNF-alpha, IL-12p40 and/or IFN-gamma expression in various organs during infection. No difference in susceptibility to experimental cryptococcosis was found between C3H/HeJ mice and C3H/HeN controls. In conclusion, our data indicate that TLR2 and MyD88, but not TLR4, critically contribute to anti-cryptococcal defenses through the induction of increased TNF-alpha, IL-12 and IFN-gamma expression.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Biondo C,Midiri A,Messina L,Tomasello F,Garufi G,Catania MR,Bombaci M,Beninati C,Teti G,Mancuso Gdoi
10.1002/eji.200425799keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
870-8issue
3eissn
0014-2980issn
1521-4141journal_volume
35pub_type
杂志文章abstract::Experimental autoimmune encephalomyelitis, an experimental murine model for multiple sclerosis, is induced by stimulation of myelin-specific T lymphocytes. Myelin oligodendrocyte glycoprotein (MOG), a minor component of myelin proteins, is a potent autoantigen which contributes extensively to the anti-myelin response....
journal_title:European journal of immunology
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doi:10.1002/eji.200535021
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abstract::Patients with X-linked lymphoproliferative syndrome type 2 (XLP-2) (BIRC4 deficiency) suffer from hyperinflammation often observed during the conditioning regimen prior to allogeneic bone marrow transplant. This article shows that in mice hematopoietic recipient cells contribute to graft-versus-host disease by the sec...
journal_title:European journal of immunology
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journal_title:European journal of immunology
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doi:10.1002/eji.1830220529
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abstract::Antigen-specific human T lymphocytes were selected by adsorption of peripheral blood mononuclear cells on monolayers of antigen-treated autologous monocytes. The enriched cell populations were propagated in interleukin 2-containing medium for 60-80 days. Their activity was tested by a proliferative response against th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131116
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journal_title:European journal of immunology
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abstract::The DC-derived chemokine CCL17, a ligand of CCR4, has been shown to promote various inflammatory diseases such as atopic dermatitis, atherosclerosis, and inflammatory bowel disease. Under steady-state conditions, and even after systemic stimulation with LPS, CCL17 is not expressed in resident splenic DCs as opposed to...
journal_title:European journal of immunology
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journal_title:European journal of immunology
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abstract::Interleukin (IL)-6 transsignaling plays a pivotal role in the shift from neutrophil to monocyte recruitment at the inflammatory site. Release of neutrophil IL-6 receptor-alpha (IL-6Ralpha, CD126) in its soluble form is a key step of IL-6 transsignaling, however, its physiological inducers are poorly known. Here, we ob...
journal_title:European journal of immunology
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doi:10.1002/1521-4141(2002010)32:10<2965::AID-IMMU2965
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abstract::Murine spleen and lymph node L3T4+ T cells were found to spontaneously produce high levels of interleukin 3 (IL3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in cultures containing 10% fetal calf serum (FCS) in the absence of other stimulation. The IL3 and GM-CSF activities in culture supernatants pe...
journal_title:European journal of immunology
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journal_title:European journal of immunology
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doi:10.1002/eji.1830221111
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050803
更新日期:1975-08-01 00:00:00
abstract::Treatment of systemic lupus erythematosus patients with epratuzumab (Emab), a humanized monoclonal antibody targeting CD22, leads to moderately reduced B-cell numbers but does not completely deplete B cells. Emab appears to induce immunomodulation of B cells, but the exact mode of action has not been defined. In the p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646383
更新日期:2016-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324076
更新日期:2003-08-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.201040704
更新日期:2010-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220634
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abstract::Gene targeting in hybridoma cells provides a tool for generating chimeric antibodies with great ease and at high yield. We present an evaluation of integration vectors for the chimerization of the immunoglobulin heavy chain locus which are universally applicable to hybridomas of different isotypes and mouse strains. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250325
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journal_title:European journal of immunology
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doi:10.1002/eji.1830260732
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abstract::The genetic control of in vitro primary syngeneic sensitization on monolayers of thyroid epithelial cells was investigated both at the level of the responder lymphocytes and that of stimulator thyroid epithelial cells using congenic resistant strains of mice. It clearly appears that similar to the in vivo model of exp...
journal_title:European journal of immunology
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doi:10.1002/eji.1830131115
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journal_title:European journal of immunology
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doi:10.1002/eji.1830270423
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260518
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abstract::Fc gamma receptor-bearing U937 cells, when incubated in serum-free buffer, were found to release spontaneously a suppressor material for pokeweed mitogen-drigen IgG synthesis which could be retained on Sepharose 4B-IgG immunosorbents. Immunosorbents coupled with IgM or F (ab')2 fragments of IgG were unable to retain t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160106
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abstract::Interphotoreceptor retinoid-binding protein (IRBP) is an immunologically privileged retinal antigen that can elicit experimental autoimmune uveitis (EAU). The nature and extent of tolerance to immunologically privileged self antigens is poorly understood. To investigate whether transgenic expression of IRBP extraocula...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200001)30:1<272::AID-IMMU272>3.0
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abstract::By comparing the restriction patterns of the DNA from 23 unrelated individuals 16 polymorphisms were defined which allowed us to differentiate between the duplicated copies Op, Ap, Lp and Od, Ad, Ld of the kappa locus (p for the C kappa proximal, d for the distal copy). Some of these duplication-differentiating polymo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210808
更新日期:1991-08-01 00:00:00
abstract::Deoxyguanosine (dGuo) has been implicated as the toxic metabolite causing a severe impairment of cellular immunity in children with a genetic deficiency of purine nucleoside phosphorylase (PNP). In peripheral blood T cells of normal donors both the pathway which leads to phosphorylation of dGuo (ultimately resulting i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160412
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180322
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199909)29:09<2658::AID-IMM
更新日期:1999-09-01 00:00:00
abstract::Mycoplasma arthritidis produces in culture a polyclonal mitogen which is active for murine and human T lymphocytes in the presence of accessory cells (AC). We studied the requirements for processing and presentation by AC of Mycoplasma arthritidis supernatant (MAS) mitogen to human T cells. As inhibitors of AC process...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181239
更新日期:1988-12-01 00:00:00
abstract::We used 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester labeled TCR-transgenic CD4(+) T cells to investigate the contribution of B7 costimulation to T cell activation and clonal expansion. B7 costimulation was blocked with the fusion protein cytotoxic T lymphocyte-associated antigen-4 (CTLA-4)-Ig, which pre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323929
更新日期:2003-08-01 00:00:00