Abstract:
:A single T cell clone can be activated by many different peptides in the context of a particular HLA molecule. To quantify the number of peptides that can be recognized by a CD4(+) T cell clone, we screened a one-bead-one-peptide synthetic peptide library and a protein database for peptides that stimulate an HLA-DR3-restricted, human glutamic acid decarboxylase (GAD65)-reactive CD4(+) T cell clone. Both the library screening and the database analysis indicated that this T cell clone is able to recognize approximately 10(6) 11-mer peptides at low nanomolar concentration. Furthermore, we determined that the frequency of cross-reactivity increased only 1.5-3 times when the peptide concentration increased 10 times, in the range of 0.01 - 1 microM. These data imply that there is a considerable potential for T cell cross-reactivity and are useful for studies on the role of molecular mimicry in the etiology of T cell-mediated disease.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Hiemstra HS,van Veelen PA,Willemen SJ,Benckhuijsen WE,Geluk A,de Vries RR,Roep BO,Drijfhout JWdoi
10.1002/(SICI)1521-4141(199908)29:08<2385::AID-IMMkeywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
2385-91issue
8eissn
0014-2980issn
1521-4141journal_volume
29pub_type
杂志文章abstract::Two out of 25 monoclonal anti-DNA autoantibodies that were produced by human-human hybridoma were found to have lymphocytotoxic activity. The antibodies reacted with normal B and T lymphocytes at cold (4 degrees C) as well as at warm (37 degrees C) temperatures. The lymphocytotoxic activity of the monoclonal anti-DNA ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151012
更新日期:1985-10-01 00:00:00
abstract::Natural killer (NK) cell activity can be stimulated by different surface receptors. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating human NK cell cytotoxicity and cytokine production. Here we show that stimulation of human NK cells by anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636146
更新日期:2006-12-01 00:00:00
abstract::Ligand binding to beta1-integrins exerts multiple effects on cells of the immune system including adhesion, spreading, haptotaxis and costimulation of T cells activated by anti-CD3. Here we show that a high-affinity ligand for beta1-integrins, the invasin (Inv) protein of Yersinia pseudotuberculosis, can induce cell d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<1129::AID-IMMU1129>3
更新日期:2002-04-01 00:00:00
abstract::Cross-linking of the high-affinity IgE receptor (FcepsilonRI) on mast cell activates signaling pathways that trigger degranulation and the release of multiple pro-inflammatory mediators. Mature,immature and precursor mast cells are degranulation competent. We show here that the signaling protein SWAP-70 has a function...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<1121::AID-IMMU1121>3
更新日期:2002-04-01 00:00:00
abstract::The nonobese diabetic (NOD) mouse is a relevant model for studying human insulin-dependent diabetes mellitus (IDDM). The selective destruction of insulin-secreting cells in this model is subsequent to an autoimmune reaction directed towards the beta cells inside the islets of Langerhans of the pancreas. Given the key ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210511
更新日期:1991-05-01 00:00:00
abstract::Treg subsets play a role in sustaining peripheral tolerance, are characterized by markers such as forkhead winged-helix transcription factor (FOXP3) and CD25, and produce suppressive cytokines, such as IL-10 and TGF-β. Glucocorticoid-induced TNF receptor family-related (GITR) protein has been suggested to regulate Tre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040943
更新日期:2011-08-01 00:00:00
abstract::Gout manifests as recurrent episodes of acute joint inflammation and pain due to the deposition of monosodium urate (MSU) crystals within the affected tissue in a process dependent on NLRP3 inflammasome activation. The synthesis, activation, and release of IL-1β are crucial for MSU-induced inflammation. The current st...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545798
更新日期:2016-01-01 00:00:00
abstract::The HIV-1 Tat protein plays a critical role in the pathogenesis of HIV and has been considered as a candidate vaccine antigen. In an effort to design a non-invasive vaccination strategy against HIV-1 that stimulates the induction of systemic and mucosal immune responses, we studied the transcutaneous delivery of a syn...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425313
更新日期:2004-12-01 00:00:00
abstract::Physical exercise, mental stress, or infusion of beta-adrenergic agonists result in an increase in the number of natural killer (NK) cells in the peripheral circulation. In view of the specific migration pattern of NK cells in vivo, it has been suggested that these cells may be released from the marginating pool in bl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231230
更新日期:1993-12-01 00:00:00
abstract::Previously we implicated c-Jun N-terminal kinase (JNK) as an element that is involved in signal integration during co-stimulation of T lymphocytes. This pathway has now been traced to an upper level, comprising MAPKK SEK1/MKK4/JNKK1 which, similarly to JNK, must receive input both from the TCR and CD28. A large portio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199808)28:08<2320::AID-IMM
更新日期:1998-08-01 00:00:00
abstract::The T cell receptor (TcR) on CD8+ T lymphocytes recognizes a complex which consists of a major histocompatibility complex (MHC) heavy chain, beta 2-microglobulin (beta 2M), and peptide on the surface of antigen-presenting cells. Mutational analyses have suggested that the TcR recognizes both the alpha 1 and alpha 2 do...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231145
更新日期:1993-11-01 00:00:00
abstract::Our understanding of the biology of human natural killer (NK) cells has significantly advanced in recent years upon identification of a family of NK cell-expressed genes that encode killer cell inhibitory receptors (KIR). Individual KIR can selectively bind various HLA class I allotypes and consequently transduce inhi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199802)28:02<599::AID-IMMU
更新日期:1998-02-01 00:00:00
abstract::We here report the cDNA sequences of 11 new rat Ly49 genes with full and three with incomplete open reading frames. Although obtained from different inbred rat strains, these as well as six previously published cDNA represent non-allelic genes matching different loci in the Brown Norway (BN) rat genome, which is predi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425429
更新日期:2005-01-01 00:00:00
abstract::Anti-immunoglobulin antibodies (anti-Ig) provoke the rapid breakdown of phosphatidylinositol bisphosphate (PIP2), elevation of cytoplasmic Ca2+ levels ([Ca2+]i) and activation of protein kinase C (PKC) in B lymphocytes. Tumor-promoting phorbol esters, like phorbol myristate acetate, also activate PKC, but inhibit anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170119
更新日期:1987-01-01 00:00:00
abstract::The in vitro immune response of murine spleen cells to the T cell-independent antigen, 2,4-dinitrophenyl-beta-alanyl-glycyl-glycyl-N(2-aminoethyl)carbamyl-methylated Ficoll (DAGG-Ficoll), requires the presence of macrophages, or 24-hr culture supernates from peritoneal cells. Complete reconstitution of the response by...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081105
更新日期:1978-11-01 00:00:00
abstract::The inflammatory process that underlies allergic diseases such as asthma is characterized by tissue infiltration of eosinophils and T cells. We have used the Stamper-Woodruff frozen-section assay to characterize the receptors involved in adhesion of human peripheral blood T cells to nasal polyp endothelium (NPE) as a ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199904)29:04<1324::AID-IMM
更新日期:1999-04-01 00:00:00
abstract::Natural killer (NK) cells in normal mouse spleen have so far been found to lack conventional cell surface markers. Recently, neuraminidase treatment has been shown to uncover receptors for the A hemagglutinin of the snail Helix pomatia (HP) on populations (preferentially T cells) of human blood lymphocytes. We have us...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081103
更新日期:1978-11-01 00:00:00
abstract::In humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonst...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948474
更新日期:2020-06-01 00:00:00
abstract::Co-culture of purified T and B cells obtained from cytochrome c-specific TCR- and hen egg lysozyme (HEL)-specific Ig-transgenic mice was used to examine the role of B cell receptor (BCR) ligation and TCR affinity on the efficiency of T-B cell collaboration. The results showed that BCR ligation of naive B cells with HE...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199812)28:12<4037::AID-IMM
更新日期:1998-12-01 00:00:00
abstract::The effector function of activated CD4(+) T cells and secretion of cytokines are important in the establishment of productive immune responses and tolerance. We identified expression by CD4(+) T cells of Notch receptors and ligands and enhanced Notch signaling upon activation. Notch1 expression was up regulated and co...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425562
更新日期:2005-03-01 00:00:00
abstract::C1q, a subcomponent of C1--the first component of complement, is synthesized by macrophages (M phi). Immunofluorescence and immunoperoxidase studies first indicated the presence of C1q on the surface of guinea pig (gp) and human peritoneal M phi (Loos, M., Storz, R., Müller, W. and Lemmel, E. M., Immunobiology 1981. 1...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230918
更新日期:1993-09-01 00:00:00
abstract::Proliferation of BCL1 cells, generally used for the measurement of murine interleukin (IL)5, is shown to be strongly suppressed by interferon (IFN)-gamma. This effect can be abrogated by the addition of anti-IFN-gamma antibodies. Thus, the IL5 activity of culture supernatants derived from T cells simultaneously produc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190726
更新日期:1989-07-01 00:00:00
abstract::We have analyzed the relationship between the signaling pathways coupled to surface immunoglobulin and interleukin (IL)-4 receptors in human B cells from the patterns of expression of a panel of phorbol ester-inducible early response genes (ERG) activated by anti-IgM and IL-4 stimulation in vitro. Anti-IgM stimulation...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231122
更新日期:1993-11-01 00:00:00
abstract::We have shown previously that complement factor H (CFH) and complement factor B (CFB) are constitutively expressed by retinal pigment epithelial cells and their production is regulated by inflammatory cytokines, suggesting that the alternative pathway (AP) of complement activation might play a role in retinal inflamma...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040323
更新日期:2010-10-01 00:00:00
abstract::The MHC-related 1 (MR1) protein is a monomorphic, evolutionarily conserved MHC class I-like molecule, which is necessary for the development and functions of mucosal-associated invariant T (MAIT) cells, a new subset of innate-like lymphocytes. Multiple isoforms of the MR1 gene are naturally transcribed, but only the f...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242461
更新日期:2013-05-01 00:00:00
abstract::CTLA-4 is a crucial immune regulator that mediates both negative costimulation signals to T cells, and regulatory T (Treg)-cell extrinsic control of effector responses. Here we present evidence supporting a novel mechanism for this extrinsic suppression, executed by the alternatively spliced soluble CTLA-4 isoform (sC...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242529
更新日期:2013-05-01 00:00:00
abstract::We have determined the cell cycle position of individual mouse leukemia L 1210 cells by cytophotometric and autoradiographic techniques and simultaneously determined the amount of histocompatibility antigens expressed on the surface of individual cells by quantitative immunofluorescence. Interferon treatment of L 1210...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060112
更新日期:1976-01-01 00:00:00
abstract::Specific monoclonal antibodies (mAb) directed against the CD23 antigen were used to study human interleukin 4 (hIL4)-induced IgE production by blood and tonsillar mononuclear cells. Both peripheral blood and tonsillar mononuclear cells stimulated by hIL4 expressed membrane CD23 as detected by the binding of all anti-C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200120
更新日期:1990-01-01 00:00:00
abstract::A murine monoclonal IgM antibody recognizes an antigen (Mo3e) found on the surface of human peripheral blood monocytes stimulated with phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide and muramyl dipeptide and blocks the monocyte response to migration inhibitory factor (MIF). We utilized Western blot and immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190423
更新日期:1989-04-01 00:00:00
abstract::Reactive oxygen species (ROS) are implicated in the modulation of diverse processes including immune responses. To evaluate the effects of metabolic ROS produced by mitochondria on B-cell function and development, we created transgenic (Tg) mice expressing a phosphorylation-defective mutant of succinate dehydrogenase ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646342
更新日期:2017-02-01 00:00:00