Abstract:
:The MHC-related 1 (MR1) protein is a monomorphic, evolutionarily conserved MHC class I-like molecule, which is necessary for the development and functions of mucosal-associated invariant T (MAIT) cells, a new subset of innate-like lymphocytes. Multiple isoforms of the MR1 gene are naturally transcribed, but only the full-length MR1A has been analyzed so far. Using transfected cell lines expressing an alternative spliced transcript, MR1B, characterized by the absence of the α3 extracellular domain, we show that MR1B is transcribed and glycosylated but remains in an immature (endoglycosidase H-sensitive) state. MR1B mostly accumulates in the ER, without interacting with proteins of the peptide-loading complex such as tapasin. Interestingly, it is nevertheless found expressed at the cell surface, independently of β2-microglobulin, in a homodimeric form. MR1B is functional as its overexpression induces MAIT cell activation in vitro in the presence of bacteria. Altogether, these data show that MR1B displays several remarkable features, and probably plays a physiological role complementary to MR1A with respect to MAIT cell development and/or function.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Lion J,Debuysscher V,Wlodarczyk A,Hodroge A,Serriari NE,Choteau L,Ouled-haddou H,Plistat M,Lassoued K,Lantz O,Treiner Edoi
10.1002/eji.201242461subject
Has Abstractpub_date
2013-05-01 00:00:00pages
1363-73issue
5eissn
0014-2980issn
1521-4141journal_volume
43pub_type
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