Abstract:
:Tumor necrosis factor (TNF) is a cytokine which, among other properties, is a principle mediator of inflammation and septic shock. It acts upon target cells by binding to specific cell surface receptors. A10G10 is a murine monoclonal antibody which recognizes human TNF and neutralizes its activity. A rabbit polyclonal antibody directed at the antigen-binding site of A10G10 was raised and affinity purified over an A10G10 column. The resultant anti-idiotypic antibody recognized not only A10G10 but also both TNF receptors. It showed TNF agonist activity in two different TNF bioassays, and competed with several anti-TNF receptor monoclonal antibodies and TNF itself for binding to cells. These results represent an example of a method for obtaining antibodies to a ligand-specific receptor in the absence of the receptor itself.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Galloway CJ,Madanat MS,Sarr T,Espevik T,Dumas ML,Mitra G,Ranges GEdoi
10.1002/eji.1830221143keywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
3045-8issue
11eissn
0014-2980issn
1521-4141journal_volume
22pub_type
杂志文章abstract::Reactive nitrogen intermediates (RNI), like nitric oxide (NO) and peroxynitrite, have antiviral effects against certain viruses. Hantaviruses, like other members of the Bunyaviridae family, have previously not been shown to be sensitive to RNI. In this study, we compared the effects of NO and peroxynitrite on hantavir...
journal_title:European journal of immunology
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更新日期:2006-10-01 00:00:00
abstract::The pathways of human anti-pig T cell xenorecognition have been investigated. Freshly isolated porcine alveolar lavage (AL) cells induced primary proliferative responses by human peripheral and cord blood mononuclear cells which were inhibited by anti-HLA-DR antibody (indirect xenorecognition). Following over-night cu...
journal_title:European journal of immunology
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doi:10.1002/eji.1830260630
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160106
更新日期:1986-01-01 00:00:00
abstract::Mice lacking the tumor suppressor gene p53 spontaneously develop T-cell lymphomas at a high rate, suggesting that in these mice lymphomas arise due to defective apoptosis mechanisms in T cells mediated by p53. However, a role of p53 in regulation of T-cell responses or apoptosis has been poorly defined. TCR-mediated s...
journal_title:European journal of immunology
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doi:10.1002/eji.200939736
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abstract::CD23-deficient and anti-CD23 monoclonal antibody-treated mice were used to investigate the role of the low-affinity receptor for IgE (CD23) in allergic airway inflammation and airway hyperresponsiveness (AHR). While there were no significant differences in ovalbumin (OVA)-specific IgE titers and tissue eosinophilia, e...
journal_title:European journal of immunology
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更新日期:1999-09-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.1830110510
更新日期:1981-05-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.1830150512
更新日期:1985-05-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2003-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210527
更新日期:1991-05-01 00:00:00
abstract::Although many B lymphocytes contain two completely rearranged immunoglobulin (Ig) heavy (H) chain genes, only one of the alleles specifies a protein product. This phenomenon is termed allelic exclusion and is controlled in part by the membrane portion of Ig H chains. We have identified a mature B cell line derived fro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210110
更新日期:1991-01-01 00:00:00
abstract::Endoglin is an homodimeric membrane antigen with capacity to bind transforming growth factor-beta (TGF-beta) and whose expression is up-regulated on myeloid cells upon differentiation to macrophages. We have isolated full-length cDNA clones from a lambda gt 10 library, prepared from phorbol 12-myristate 13-acetate-dif...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230943
更新日期:1993-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230835
更新日期:1993-08-01 00:00:00
abstract::Glycosylphosphatidylinositol-anchored (GPI)-Db molecules are defective in mediating cytotoxic T lymphocytes (CTL) lysis of transfected lymphoma cells, compared to their transmembrane (TM) counterpart. This defect is manifest when antigenic peptide must be processed and presented through the endogenous pathway. These s...
journal_title:European journal of immunology
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doi:10.1002/eji.1830260938
更新日期:1996-09-01 00:00:00
abstract::Prostaglandins (PG) are important modulators of immune and inflammatory responses. We recently demonstrated that the production of PGD(2) by the helminthic parasite Schistosoma mansoni inhibits the migration of epidermal Langerhans cells (LC) to the draining lymph nodes (DLN). Here, we identify the responsible parasit...
journal_title:European journal of immunology
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更新日期:2003-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160411
更新日期:1986-04-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.201646730
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journal_title:European journal of immunology
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doi:10.1002/1521-4141(200002)30:2<626::AID-IMMU626>3.0
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1995-11-01 00:00:00
abstract::Membrane-bound immunoglobulins of the IgM and IgD class are expressed on the B cell surface in association with a disulfide-linked heterodimer consisting of alpha and beta subunits. While the alpha component of the IgM antigen receptor (IgM-alpha, 34 kDa) is encoded by the B cell-specific gene mb-1, the gene coding fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211012
更新日期:1991-10-01 00:00:00
abstract::Targeting exogenous antigen into the MHC class I-restricted presentation pathway is a prerequisite for the induction of cytotoxic T lymphocytes (CTL) which have been shown to represent an important component of the protective and therapeutic immune response to viral infections and tumors. In this study, we produced re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2726::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::We have demonstrated that analogues of alpha-mannosyl ceramide (alpha-ManCer) consisting of a series of immunosuppressive 2-aminoalcohol derivatives in place of sphingosine promote a greater immune response from mouse invariant Valpha19-Jalpha26 (AV19-AJ33) TCR-bearing NKT (Valpha19 NKT) cells than alpha-ManCer itself...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection frequently develops into liver disease and is accompanied by extra-hepatic autoimmune manifestations. The tetraspanin CD81 is a putative HCV receptor as it binds the E2 envelope glycoprotein of HCV and bona fide HCV particles. Here we show that HCV E2 binding to CD81 on human ...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221131
更新日期:1992-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1989-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261241
更新日期:1996-12-01 00:00:00
abstract::The human type I interferon (IFN) receptor was characterized by ligand blotting. In this method, plasmalemma proteins or detergent-lysed whole-cell extracts from human Burkitt lymphoma cell lines were separated on polyacrylamide gels and subsequently transferred onto nitrocellulose sheets. Probing the blots with 3 x 1...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181221
更新日期:1988-12-01 00:00:00
abstract::High BDCA3 expression is associated with a specific human IFN-λ-producing dendritic cell (DC) subset. However, BDCA3 has also been detected on other DC subsets. Thus far, development and function of BDCA3 expression on DCs remains poorly understood. Human Langerhans cells (LCs) and interstitial DCs (intDCs) can be gen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444802
更新日期:2015-05-01 00:00:00