Abstract:
:The fate of the T cell receptor (TcR)/CD3 complex was examined on a cytotoxic T lymphocyte (CTL) clone (KB5.C20) activated either via binding of an anti-TcR monoclonal antibody (mAb) or by a Ca2+ ionophore and phorbol 12-myristate 13-acetate (PMA). After binding of the anti-TcR mAb, electron microscopy revealed internalization through coated vesicles followed by slow degradation of the antibody as shown by use of radiolabeled mAb. The influence of activation on TcR/CD3 internalization was analyzed. The Ca2+ ionophore alone had no effect on internalization, whereas PMA induced an accelerated internalization of anti-TcR mAb. PMA-induced internalization was dependent on protein kinase C (PKC) as shown by its absence in PKC-depleted cells or in the presence of the PKC inhibitor staurosporine. Anti-TcR mAb-induced internalization was maintained in PKC-depleted cells, but unexpectedly remained sensitive to inhibition by staurosporine. The monovalent anti-TcR mAb Fab fragment is non-stimulatory for the CTL. It was poorly internalized but its internalization was induced by PMA. Surprisingly, on PKC-depleted cells, the Fab was internalized more readily than in untreated cells and this internalization was sensitive to inhibition by staurosporine. Inhibition of PMA-induced phosphorylation of gamma and epsilon subunits of CD3 was demonstrated after depletion of PKC or in the presence of staurosporine, confirming that PKC function was inhibited in those conditions. Cross-linking of the TcR via plastic-coated anti-TcR mAb led to phosphorylation of CD3 gamma and epsilon and also of zeta, known to be phosphorylated on tyrosines. All of these phosphorylation events were inhibited by treatment with staurosporine. Our results indicate that staurosporine inhibits the receptor internalization induced by anti-TcR mAb by means other than inhibition of PKC, suggesting that other kinases may control a step of this internalization process.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Boyer C,Auphan N,Luton F,Malburet JM,Barad M,Bizozzero JP,Reggio H,Schmitt-Verhulst AMdoi
10.1002/eji.1830210707subject
Has Abstractpub_date
1991-07-01 00:00:00pages
1623-34issue
7eissn
0014-2980issn
1521-4141journal_volume
21pub_type
杂志文章abstract::GTPase immune-associated proteins (Gimap) genes encode evolutionarily conserved GTP-binding proteins that are preferentially expressed in immune cells. Specific members have been shown to be involved in lymphocyte development, or are associated with inflammatory and autoimmune diseases. However, the function of these ...
journal_title:European journal of immunology
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abstract::Natural killer T (NKT) lymphocytes rapidly produce several cytokines, including IL-4 and IFN-gamma, upon activation, and act as regulatory cells at an early interphase of innate and adaptive immune responses. They have been implicated as important elements in diverse immune responses including the regulation of autoim...
journal_title:European journal of immunology
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doi:10.1002/eji.200323963
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1995-06-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
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更新日期:1986-05-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1991-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 评论,杂志文章
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:2003-01-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1995-07-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1995-02-01 00:00:00
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journal_title:European journal of immunology
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abstract::Murine cytolytic T cell lines have been analyzed for the expression of two surface glycoproteins called T145 and T130. T145, known to be expressed by activated cytolytic T cells, is also expressed by such lines, but T130, which has been described by a universal T cell marker, is not. Our results suggest a structural r...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1980-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2014-02-01 00:00:00