Abstract:
:We have analyzed the relationship between the signaling pathways coupled to surface immunoglobulin and interleukin (IL)-4 receptors in human B cells from the patterns of expression of a panel of phorbol ester-inducible early response genes (ERG) activated by anti-IgM and IL-4 stimulation in vitro. Anti-IgM stimulation led to the induction of all eleven ERG tested. Two of these, the proto-oncogene, c-fos and an anonymous ERG 1R20 were insensitive to protein kinase C (PKC) inhibition with the drug, staurosporine and retained inducibility after down-regulation of PKC activity by purging with phorbol ester. These observations are consistent with previous data showing anti-IgM signaling through both PKC-dependent and PKC-independent pathways. c-fos and 1R20 were also the only ERG inducible in response to IL-4 stimulation and whilst ionomycin induced only c-fos, dibutyryl cyclic adenosine monophosphate stimulation led to induction of both c-fos and 1R20. These observations lend support to a role for the adenylate cyclase pathway being important for coupling of IL-4-generated signals to B cells responses. None of the anti-IgM-responsive ERG was further induced when B cells were co-stimulated with a combination of anti-IgM and IL-4, suggesting that the signaling cascades from these two agents are integrated downstream of third messenger pathways to synergistically promote B cell proliferation.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Murphy JJ,Norton JDdoi
10.1002/eji.1830231122subject
Has Abstractpub_date
1993-11-01 00:00:00pages
2876-81issue
11eissn
0014-2980issn
1521-4141journal_volume
23pub_type
杂志文章abstract::T-cell homeostasis preserves the numbers, the diversity and functional competence of different T-cell subsets that are required for adaptive immunity. Naïve CD4(+) T (TN ) cells are maintained in the periphery via the common γ-chain family cytokine IL-7 and weak antigenic signals. However, it is not clear how memory C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444701
更新日期:2014-10-01 00:00:00
abstract::We investigated whether percutaneous sensitization with different allergens through barrier-disrupted skin regulates the balance of Th1/Th2 cytokine expression. When mice were sensitized with the typical hapten picryl chloride (PiCl) by a single topical application to intact skin, there was an up-regulation in the lym...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<769::AID-IMMU
更新日期:1998-03-01 00:00:00
abstract::The diversity of the human immune repertoire and how it relates to a functional immune response has not yet been studied in detail in humanized NOD.SCID.gammac(-/-) immunodeficient mice. Here, we used a multiplex PCR on genomic DNA to quantify the combinatorial diversity of all possible V-J rearrangements at the TCR-b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939480
更新日期:2009-08-01 00:00:00
abstract::Interleukin-31 (IL-31) is a Th2 cell-derived cytokine that has been closely linked to pruritic skin inflammation. More recently, enhanced IL-31 serum levels have also been observed in patients with allergic rhinitis and allergic asthma. Therefore, the main aim of this study was to unravel the contribution of IL-31 to ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048547
更新日期:2020-07-10 00:00:00
abstract::The multimeric Fc gamma RIIIA (CD16) complex is expressed on the surface of natural killer (NK) cells and is composed of a 50-70-kDa transmembrane glycoprotein Fc gamma receptor (CD16), the T cell receptor (TCR)-zeta chain, and the Fc epsilon RI gamma chain. Cross-linking Fc gamma RIIIA initiates the rapid tyrosine ph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231017
更新日期:1993-10-01 00:00:00
abstract::Co-culture of purified T and B cells obtained from cytochrome c-specific TCR- and hen egg lysozyme (HEL)-specific Ig-transgenic mice was used to examine the role of B cell receptor (BCR) ligation and TCR affinity on the efficiency of T-B cell collaboration. The results showed that BCR ligation of naive B cells with HE...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199812)28:12<4037::AID-IMM
更新日期:1998-12-01 00:00:00
abstract::PA28alpha/beta is a regulatory complex of the 20S proteasome which consists of two IFN-gamma inducible subunits. Both subunits, alpha and beta, contribute equally to the formation of hexa- or heptameric rings which can associate with the 20S proteasome. Previously, we have shown that overexpression of the PA28alpha su...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>
更新日期:2000-12-01 00:00:00
abstract::Somatic hypermutation in B cells undergoing T cell dependent immune responses generates high affinity antibodies that provide protective immunity. B cells also switch from the expression of immunoglobulin (Ig) M and IgD to that of other Ig classes through somatic DNA recombination. Recent work has implicated DNA stran...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200202)32:2<316::AID-IMMU316>3.0
更新日期:2002-02-01 00:00:00
abstract::In this study the genetic control of major histocompatibility complex (MHC) class II gene expression during the transition from B cell to plasma cell has been analyzed. Class II molecules are not expressed in plasma cells because of an active suppression resulting in the abrogation of class II gene transcription. We s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261028
更新日期:1996-10-01 00:00:00
abstract::Adoptive T-cell therapy (ATCT) can result in tumor rejection, yet the behavior and fate of the introduced T cells remain unclear. We developed a novel bioluminescence mouse model, which enabled highly sensitive detection of T-cell signals at the single-cell level. Transferred T cells preferentially accumulated within ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141452
更新日期:2011-11-01 00:00:00
abstract::This study was performed as a means to examine the effects of environment and stem cell origin on the molding of T cell receptor gene rearrangements during T cell development. Lethally irradiated adult mice were first reconstituted with (C57BL/6 x DBA/2)F1 stem cells, which derived either from a fetal liver or adult b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211208
更新日期:1991-12-01 00:00:00
abstract::As discussed in this review, human cytomegalovirus (HCMV) infection in healthy individuals is associated with a variable and persistent increase of NK cells expressing the CD94/NKG2C activating receptor. The expansion of NKG2C(+) NK cells reported in other infectious diseases is systematically associated with HCMV co-...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201243117
更新日期:2013-05-01 00:00:00
abstract::Natural resistance to hemopoietic allograft results in functional elimination of the graft from the host within 24 to 48 h. The resistance is specific and is directed to the cell surface target structures of an unidentified nature. These determinants are controlled by the major histocompatibility complex-linked hemopo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190702
更新日期:1989-07-01 00:00:00
abstract::The contribution of the costimulatory molecules B7-1 and B7-2 to the in vivo differentiation of Th cells remains controversial. The infection of resistant and susceptible strains of mice with the parasite Leishmania major provides a well-established model for studying in vivo differentiation of CD4+ T cells. We have i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1764::AID-IMMU1764>3
更新日期:2002-06-01 00:00:00
abstract::Activation-induced deaminase (AID) is the master regulator of class switch recombination (CSR) and somatic hypermutation (SHM), but the mechanisms regulating AID function are obscure. The differential pattern of switch plasmid activity in three IgM(+)/AID(+) and two IgG(+)/AID(+) B cell lines prompted an analysis of g...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737664
更新日期:2008-01-01 00:00:00
abstract::Gammadelta T cells are expanded in human IgA nephropathy and in a rat model of adriamycin (ADR)-induced nephropathy. Despite different diseases and species, these renal gammadelta T cells use a restricted set of gammadelta T cell receptor (TCR) genes. To explore whether this phenomenon of post injury expansion of gamm...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324780
更新日期:2004-08-01 00:00:00
abstract::Previous studies from our laboratory have shown that prolonged exposure of mouse macrophages to IFN-beta interferes with their subsequent ability to become activated for tumor cell killing. Data reported here show that such inhibition is due to reduced production of NO, resulting from decreased transcription of the ge...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200006)30:6<1551::AID-IMMU1551>3
更新日期:2000-06-01 00:00:00
abstract::Whereas the role of CD4 T cells in B cell memory generation is well established and unequivocal, the role that CD4 T cells play in CD8 responses was until recently far more elusive and controversial. A series of recent reports, however, have re-assessed the role of CD4 help on CD8 responses and have given rise to surp...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200324576
更新日期:2003-12-01 00:00:00
abstract::IFN-γ-activated keratinocytes are key contributors to the pathogenetic mechanisms leading to type-1 immune-mediated skin disorders. In these epidermal cells, SOCS1 negatively regulates the molecular cascades triggered by IFN-γ by disabling JAK2 phosphorylation through its kinase inhibitory region (KIR). Aimed at poten...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343370
更新日期:2013-07-01 00:00:00
abstract::Atopic dermatitis (AD) or eczema is the most common chronic inflammatory skin disease. It is a multifactorial disease with local and systemic immune changes. Current therapies focus on restoring the local skin barrier or inhibiting immune responses. In this issue of the European Journal of Immunology, Sehra et al. [Eu...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201646708
更新日期:2016-11-01 00:00:00
abstract::Cooperation between B cells specific for an antigen exposed on a viral structure and T helper (Th) cells specific for an internal antigen, as demonstrated with influenza, hepatitis B and rabies viruses, has been termed intrastructural help. Th cells specific for internal proteins of HIV, which are much less mutated th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250513
更新日期:1995-05-01 00:00:00
abstract::Transgenic mice in which mouse interleukin (IL)-7 cDNA is expressed under the control of the mouse major histocompatibility complex (MHC) class II (E alpha) promoter develop a lymphoproliferative disease characterized by the early polyclonal expansion of T cells followed in many cases by the development of lymphomas o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260105
更新日期:1996-01-01 00:00:00
abstract::Regulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we character...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747041
更新日期:2017-12-01 00:00:00
abstract::As we approach the centenary of Elie Metchnikoff's Nobel Prize (1908), it is opportune to reflect upon the history of macrophage immunobiology, take stock of current knowledge and anticipate questions for the future. Starting from his appreciation of phagocytosis as an important determinant of host defence against inf...
journal_title:European journal of immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1002/eji.200737638
更新日期:2007-11-01 00:00:00
abstract::Deleted in malignant brain tumors 1 (DMBT1) is a secreted glycoprotein displaying a broad bacterial-binding spectrum. Recent functional and genetic studies linked DMBT1 to the suppression of LPS-induced TLR4-mediated NF-kappaB activation and to the pathogenesis of Crohn's disease. Here, we aimed at unraveling the mole...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838689
更新日期:2009-03-01 00:00:00
abstract::Human T cell clones expressing the T cell receptor (TcR) gamma/delta were isolated from peripheral blood lymphocytes of two unrelated donors. The TcR gamma/delta+ clones derived from one of these donors were all of the Ti gamma A+, delta-TCS1-, BB3+ phenotype indicating the exclusive use of the V gamma 9 and V delta 3...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191120
更新日期:1989-11-01 00:00:00
abstract::Differential screening of a subtracted cDNA library led to the detection of two distinct but homologous mouse cDNA, called CTLA-2 alpha and CTLA-2 beta. The corresponding transcripts have a tissue distribution restricted to T lymphocytes, where they are inducible upon activation, and to mast cells. The open-frame regi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190409
更新日期:1989-04-01 00:00:00
abstract::Respiratory syncytial virus (RSV) causes severe bronchiolitis in infants worldwide. The immunological factors responsible for RSV susceptibility in infants are poorly understood. Here, we used the BALB/c mouse model of neonatal RSV infection to study the mechanisms leading to severe disease upon reexposure to the viru...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545929
更新日期:2016-04-01 00:00:00
abstract::We isolated immunoglobulin (Ig) VH4 genes that were rearranged in the genomic DNA of 160 day human fetal spleen. Productively rearranged VH 4-21 genes were cloned into pRTM1, a human IgM expression vector. This allowed us to generate IgM kappa-expressing transfectomas by co-transfecting each of these constructs with p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241204
更新日期:1994-12-01 00:00:00
abstract::Plasmodium falciparum stimulates peripheral blood gamma delta + T cells from unexposed donors. The responding cells bear V gamma 9+ chains of the T cell receptor, the majority of which, but not all, are associated with V delta 2 chains. We have analyzed whether the precursor frequency of these V gamma 9+ cells approac...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221045
更新日期:1992-10-01 00:00:00