Abstract：
:Regulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we characterized TCR signalling and recruitment of TCR signalling components to the immunological synapse (IS) in Treg cells and Tconv cells. With the exception of Themis which we detected in lower amounts in Treg cells, other major TCR signalling components were found equally abundant, however, their phosphorylation-status notably discriminates Treg cells from Tconv cells. Overall, this study identified 121 Treg cell-specific phosphorylations. Short-term triggering of T cell subsets via CD3 and CD28 widely harmonized these variations with the exception of eleven TCR signalling components that mainly regulate cytoskeleton dynamics and molecular transport. Accordingly, conjugation with B cells indeed caused variant cellular morphology and revealed a Treg cell-specific recruitment of TCR signalling components such as PKCθ, PLCγ1 and ZAP70 as well as B cell-derived CD86 into the IS. Together, results from this study support the existence of a Treg cell-specific IS and suggest Treg cell-specific cytoskeleton dynamics as a novel determinant for the unique functional properties of Treg cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
van Ham M,Teich R,Philipsen L,Niemz J,Amsberg N,Wissing J,Nimtz M,Gröbe L,Kliche S,Thiel N,Klawonn F,Hubo M,Jonuleit H,Reichardt P,Müller AJ,Huehn J,Jänsch Ldoi
10.1002/eji.201747041subject
Has Abstractpub_date
2017-12-01 00:00:00pages
2043-2058issue
12eissn
0014-2980issn
1521-4141journal_volume
47pub_type
杂志文章abstract：:Indirect immunofluorescence revealed that 13% of BALB/c and 33% of CBA spleen cells of B type carry specific binding sites at their surface for double-stranded poly(I).poly(C). Pretreatment of BALB/c spleen cells with anti-mouse immunoglobulin serum increased the number of B cells capable of binding poly(I).poly(C) in...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830081213
更新日期：1978-12-01 00:00:00
abstract：:A significant proportion of memory B cells home to bone marrow (BM) which is a major site of anamnestic antibody responses in mice. We hypothesized that memory T cells likewise accumulate in BM perhaps to provide help for antibody production, and that the compartment of CD4+ T cells in BM of unimmunized mice would be ...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/(SICI)1521-4141(199903)29:03<1051::AID-IMM
更新日期：1999-03-01 00:00:00
abstract：:The variable region nucleotide sequences of seven (five IgM and two IgG) anti-histone monoclonal antibodies from a single MRL/Mp-lpr/lpr mouse have been determined. These antibodies are not clonally related and used diverse V, D and J genes. However, six of the seven antibodies have VH segments encoded by genes from t...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830210721
更新日期：1991-07-01 00:00:00
abstract：:The involvement of Lyt-2+ T cells in the immunological control of cutaneous leishmaniasis was assessed by studying the course of the disease in mice in which the number of these cells was reduced by treatment with anti-Lyt-2 monoclonal antibody (mAb). Administration of anti-Lyt-2 mAb exacerbated Leishmania major-induc...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830171007
更新日期：1987-10-01 00:00:00
abstract：:Following immunization with a complex antigen, a B cell internalizes this antigen through an interaction between its surface immunoglobulins and an epitope of the antigen. Enzymatic processing of the antigen frees one or more short peptide determinants (TD) which bind to class II molecules of the B cell. If the comple...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830210633
更新日期：1991-06-01 00:00:00
abstract：:C1q, a subcomponent of C1--the first component of complement, is synthesized by macrophages (M phi). Immunofluorescence and immunoperoxidase studies first indicated the presence of C1q on the surface of guinea pig (gp) and human peritoneal M phi (Loos, M., Storz, R., Müller, W. and Lemmel, E. M., Immunobiology 1981. 1...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830230918
更新日期：1993-09-01 00:00:00
abstract：:Deleted in malignant brain tumors 1 (DMBT1) is a secreted glycoprotein displaying a broad bacterial-binding spectrum. Recent functional and genetic studies linked DMBT1 to the suppression of LPS-induced TLR4-mediated NF-kappaB activation and to the pathogenesis of Crohn's disease. Here, we aimed at unraveling the mole...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200838689
更新日期：2009-03-01 00:00:00
abstract：:Intravenous immunoglobulins (IVIgs) efficiently suppress a variety of autoimmune diseases. Over the past few years several potential mechanisms underlying this antiinflammatory activity have become apparent. Among these, terminal sialic acid residues in the sugar moiety of the immunoglobulin G constant fragment have b...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.201242710
更新日期：2012-12-01 00:00:00
abstract：:The recognition of lipid antigens by T cells is a complex and fascinating phenomenon. The MHC-like molecules of the CD1 family have evolved to present a wide variety of both self and foreign lipids for recognition by T-cell receptors. While much progress has been made in our understanding of the NKT cells that recogni...
journal_title：European journal of immunology
pub_type： 评论,杂志文章
doi：10.1002/eji.201141408
更新日期：2011-03-01 00:00:00
abstract：:NKp46 is a novel triggering receptor expressed by all human NK cells that is involved in natural cytotoxicity. In this study we show that the surface density of NKp46 may vary in different NK cells and that a precise correlation exists between the NKp46 phenotype of NK clones and their natural cytotoxicity against HLA...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/(SICI)1521-4141(199905)29:05<1656::AID-IMM
更新日期：1999-05-01 00:00:00
abstract：:Signal transducer and activator of transcription 3 (STAT3) triggered production of Th-17 cytokines mediates protective immunity against fungi. Mutations affecting the STAT3/interleukin 17 (IL-17) pathway cause selective susceptibility to fungal (Candida) infections, a hallmark of chronic mucocutaneous candidiasis (CMC...
journal_title：European journal of immunology
pub_type： 临床试验,杂志文章
doi：10.1002/eji.201445344
更新日期：2015-10-01 00:00:00
abstract：:The role of B cells and antibody in the pathogenesis of experimental autoimmune encephalomyelitis (EAE) remains controversial. We previously demonstrated that B cells are required for EAE to be induced by the 120-amino acid extracellular domain of myelin oligodendrocyte glycoprotein (MOG). In the present study, the ro...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/1521-4141(200207)32:7<1905::AID-IMMU1905>3
更新日期：2002-07-01 00:00:00
abstract：:In June 2009, the National Institute of Allergy and Infectious Diseases (NIAID), Division of Allergy, Immunology and Transplantation (DAIT), sponsored a workshop entitled Mast Cells in Innate and Adaptive Immunity. International experts in mast cell biology discussed recent advances in the field and future areas of re...
journal_title：European journal of immunology
pub_type：
doi：10.1002/eji.200990325
更新日期：2010-01-01 00:00:00
abstract：:In a search for proteins involved in signal transduction through the T cell receptor (TcR/CD3 complex), a recently developed highly efficient method for labeling of nucleotide binding proteins in permeabilized cells was applied. Here, we report that human CD3-gamma could be labeled by periodate-oxidized [alpha-32P] GT...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830230224
更新日期：1993-02-01 00:00:00
abstract：:T cells are known to play an important role in beta cell destruction in the nonobese diabetic (NOD) mouse model of Type I diabetes and islet-specific T cell clones have been demonstrated to be capable of adoptive transfer of diabetes. One important issue involves the identity of beta cell antigens that are recognized ...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830250430
更新日期：1995-04-01 00:00:00
abstract：:The number of patients with severe atopic dermatitis (AD) has been on the rise recently. We are therefore urgently in need of a treatment that can suppress Th2 cell-mediated responses in an Ag-specific fashion. Oligodeoxynucleotides (ODN)containing CpG motifs (CpG ODN) have been highlighted as immunomodulators that re...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200526274
更新日期：2005-11-01 00:00:00
abstract：:About one hundred immunologists recently met in Capo Caccia, Sardinia, for the first of three events that will comprise the EMBO Conference Series, organised by the ENII, on Molecular and Cellular Mechanisms of Immune Regulation. The 2007 Conference focused on how immunity is shaped in healthy and diseased tissues. ...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200790031
更新日期：2007-08-01 00:00:00
abstract：:Inflammatory bowel diseases (IBD) are chronic conditions that result from uncontrolled intestinal inflammation. Pathogenic Th17 cells, characterized by production of IL-17A in the absence of IL-10, are thought to contribute to this inflammation, but in humans, antibody-mediated blockade of IL-17A is an ineffective IBD...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.201948435
更新日期：2020-09-01 00:00:00
abstract：:The in vitro immune response of murine spleen cells to the T cell-independent antigen, 2,4-dinitrophenyl-beta-alanyl-glycyl-glycyl-N(2-aminoethyl)carbamyl-methylated Ficoll (DAGG-Ficoll), requires the presence of macrophages, or 24-hr culture supernates from peritoneal cells. Complete reconstitution of the response by...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830081105
更新日期：1978-11-01 00:00:00
abstract：:Peritoneal mast cells (PMC) and intestinal mucosal mast cells (IMMC) were purified from rats infected with the nematode Nippostrongylus brasiliensis. Overall protein constituents of both mast cell subtypes were analyzed by two-dimensional gel electrophoresis using either nonequilibrium pH gradient electrophoresis (NEP...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830200911
更新日期：1990-09-01 00:00:00
abstract：:Interleukin-HP1 (HP1) is a murine T cell-derived lymphokine, originally described as a growth factor for B cell hybridomas and plasmacytomas, that was recently shown to stimulate growth and differentiation of normal B and T lymphocytes. Here, we describe a cDNA for HP1 that was isolated from a library prepared using m...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830180202
更新日期：1988-02-01 00:00:00
abstract：:Macrophage scavenger receptors are trimeric integral membrane glycoproteins which have been implicated in various macrophage functions including uptake of oxidized lipoprotein and the serum-dependent, divalent cation-independent adhesion of macrophages to tissue culture-treated plastic. In this study we have used a re...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830250224
更新日期：1995-02-01 00:00:00
abstract：:HLA class II association with insulin-dependent diabetes mellitus (IDDM) is well established but is still difficult to map to a particular locus. Polymorphism of the genes coding for transporter associated with antigen processing (TAP1 and TAP2), and located in the HLA class II region, was studied in 167 IDDM patients...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830230808
更新日期：1993-08-01 00:00:00
abstract：:Although anti-inflammatory properties of glucocorticoids (GC) are well documented, their activity in allergic diseases is still controversial. Recently, it has been reported that GC can increase, both in vivo and in vitro, the polyclonal production of total IgE. In this study we investigated the effects of GC on the a...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830270933
更新日期：1997-09-01 00:00:00
abstract：:Interferon regulatory factor-1 (IRF-1) is a transcription factor regulating the expression of several cytokines. Using IRF-1 knockout (KO) mice, the role of IRF-1 in the production and activity of IL-18 was evaluated. Administration of IL-12 or concanavalin A significantly increased levels of circulating IL-18 in wild...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/1521-4141(200102)31:2<369::aid-immu369
更新日期：2001-02-01 00:00:00
abstract：:We show that, in vitro, the response of thymocytes to certain stimuli, and their survival largely depend on the nature of the culture environment, i.e. whether thymocytes are stimulated within intact thymus lobes or in cell suspension. Exposure of isolated thymocytes to 12-O-tetra-decanoylphorbol 13-acetate (TPA)+iono...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830221011
更新日期：1992-10-01 00:00:00
abstract：:A previously designed isotopic in vivo method for measuring delayed hypersensitivity was used with nonantigenic (or weakly antigenic) phlogogenic substances. A strong reaction was observed in some circumstances, witnessing the fact that the method has a specific and a nonspecific component. The reaction is drastically...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830060606
更新日期：1976-06-01 00:00:00
abstract：:Patients undergoing allogeneic bone marrow transplantation offer a unique system to analyze NK cell development in vivo. We analyzed NK cells from 23 such patients to assess the acquisition of activating receptors. Four patients displayed an immature NK cell surface phenotype at engraftment, as their cells were CD16(-...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200324668
更新日期：2004-02-01 00:00:00
abstract：:Activation pathways inducing the expression of the interferon (IFN)-gamma gene in a cytotoxic T lymphocyte (CTL) clone were studied for their effects on transcription and on mRNA stability. IFN-gamma was secreted by the CTL clone in response to the Ca2+ ionophore ionomycin when used in conjunction with either protein ...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.1830250405
更新日期：1995-04-01 00:00:00
abstract：:In recent years, factors potentially involved in pathogenesis of rheumatoid arthritis have mostly been identified by studying well-defined patient cohorts. Characterization of antibodies from sera of affected patients, family-based heritability studies, genome-wide association scans, the analysis of environmental fact...
journal_title：European journal of immunology
pub_type： 杂志文章
doi：10.1002/eji.200939514
更新日期：2009-08-01 00:00:00
