Abstract:
:NKp46 is a novel triggering receptor expressed by all human NK cells that is involved in natural cytotoxicity. In this study we show that the surface density of NKp46 may vary in different NK cells and that a precise correlation exists between the NKp46 phenotype of NK clones and their natural cytotoxicity against HLA-class I-unprotected allogeneic or xenogeneic cells. Thus, NKp46bright clones efficiently lysed human and murine tumor cells while NKp46dull clones were poorly cytolytic against both types of target cells. We also show that the NKp46 phenotype of NK clones correlates with their ability to lyse HLA-class I-unprotected autologous cells. Finally, NKp46 was found to be deeply involved in the natural cytotoxicity mediated by freshly derived NK cells. This was indicated both by the inhibition of cytolysis after monoclonal antibody-mediated masking of NKp46 and by the correlation existing between the natural cytotoxicity of fresh NK cells derived from different donors and their NKp46 phenotype. In conclusion, these studies strongly support the concept that NKp46 plays a central role in the physiological triggering of NK cells and, as a consequence (in concert with killer inhibitory receptors), in the NK-mediated clearance of abnormal cells expressing inadequate amounts of HLA-class I molecules.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Sivori S,Pende D,Bottino C,Marcenaro E,Pessino A,Biassoni R,Moretta L,Moretta Adoi
10.1002/(SICI)1521-4141(199905)29:05<1656::AID-IMMkeywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
1656-66issue
5eissn
0014-2980issn
1521-4141journal_volume
29pub_type
杂志文章abstract::The N-terminal amino acid sequence (26 residues) of a delta-chain of a human myeloma (Er I) is homologous with the prototype sequence of the human VHIII subgroup. This indicates that the delta-chain is using for its V region the same pool of V genes as the rest of the immunoglobulin classes. Implications of this findi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060315
更新日期:1976-03-01 00:00:00
abstract::The diversity of cytokine production patterns displayed by T cells activated in vivo was investigated by analyzing short-term antigen-specific CD4+ T cell clones and single CD4+ T cells derived from draining lymph nodes of mice undergoing a T helper 2 (Th2)-like response to keyhole limpet hemocyanin (KLH). On average,...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250506
更新日期:1995-05-01 00:00:00
abstract::Antioxidant systems maintain cellular redox homeostasis. The thioredoxin-1 (Trx1) and the glutathione (GSH)/glutaredoxin-1 (Grx1) systems are key players in preserving cytosolic redox balance. In fact, T lymphocytes critically rely on reducing equivalents from the Trx1 system for DNA biosynthesis during metabolic repr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848044
更新日期:2019-05-01 00:00:00
abstract::Activation-induced deaminase (AID) is the master regulator of class switch recombination (CSR) and somatic hypermutation (SHM), but the mechanisms regulating AID function are obscure. The differential pattern of switch plasmid activity in three IgM(+)/AID(+) and two IgG(+)/AID(+) B cell lines prompted an analysis of g...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737664
更新日期:2008-01-01 00:00:00
abstract::Using sheep erythrocytes (SRBC) as the antigen, two subpopulations of spleen antigen-binding lymphocytes could be distinguished by a marked difference in the susceptibility of their receptors to trypsin. In unimmunized animals, 30% of the antigen-binding cells were trypsin-resistant, whereas at 5 days after immunizati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050612
更新日期:1976-06-01 00:00:00
abstract::The studies described in this report were designed to investigate factors that could influence the behavior of erythrocytes following their interaction with monoclonal antibodies (mAb) in a fully homologous experimental opsonization system in vivo. The clearance profiles and tissue distribution of target erythrocytes ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210413
更新日期:1991-04-01 00:00:00
abstract::T-cell homeostasis preserves the numbers, the diversity and functional competence of different T-cell subsets that are required for adaptive immunity. Naïve CD4(+) T (TN ) cells are maintained in the periphery via the common γ-chain family cytokine IL-7 and weak antigenic signals. However, it is not clear how memory C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444701
更新日期:2014-10-01 00:00:00
abstract::As discussed in this review, human cytomegalovirus (HCMV) infection in healthy individuals is associated with a variable and persistent increase of NK cells expressing the CD94/NKG2C activating receptor. The expansion of NKG2C(+) NK cells reported in other infectious diseases is systematically associated with HCMV co-...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201243117
更新日期:2013-05-01 00:00:00
abstract::Peptide ligands presented by MHC class I molecules are generated in a cascade of proteolytic events starting with the proteasome in the cytosol and frequently terminating with trimming aminopeptidases in the endoplasmic reticulum. Several cytosolic proteases can carry out intermediate proteolytic steps between these s...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200838181
更新日期:2008-03-01 00:00:00
abstract::The T cell repertoire of BALB/c mice contains clones capable of recognizing p-azobenzenearsonate (ABA)-tyrosine (Tyr) in association with both I-A and I-E-encoded class II molecules. Immunization of BALB/c animals with ABA-GAT (terpolymer of L-Glu60-L-Ala30-L-Tyr10) or ABA-GLT (terpolymer of L-Glu51-L-Lys34-L-Tyr15) i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151214
更新日期:1985-12-01 00:00:00
abstract::Activation pathways inducing the expression of the interferon (IFN)-gamma gene in a cytotoxic T lymphocyte (CTL) clone were studied for their effects on transcription and on mRNA stability. IFN-gamma was secreted by the CTL clone in response to the Ca2+ ionophore ionomycin when used in conjunction with either protein ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250405
更新日期:1995-04-01 00:00:00
abstract::Although potentially autoreactive T cells are present even in healthy subjects, most individuals do not develop autoimmune disease. It has been well demonstrated that CD4+ CD25+ regulatory T cells play a significant role in controlling the expansion of autoreactive T cells in the periphery. However, some healthy indiv...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636383
更新日期:2007-01-01 00:00:00
abstract::In this study, we demonstrated that antibodies reactive with a common beta 1 subunit (CD29) of the VLA family of antigens could synergize with CD3 but not CD2 in inducing CD4 cell proliferation in a serum-free culture system. The extent of activation was similar to that obtained when CD4 cells were cultured with anti-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210212
更新日期:1991-02-01 00:00:00
abstract::The C-terminal fragment of merozoite surface protein-1 (MSP-1) of the mouse malaria parasite Plasmodium chabaudi chabaudi (AS) stimulates a weak CD4 T cell response when compared to the response to a more structurally simple region of the molecule. The tertiary structure of the C-terminal region of MSP-1 is maintained...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200101)31:1<72::aid-immu72
更新日期:2001-01-01 00:00:00
abstract::Ptak and Askenase showed that both alphabeta and gammadelta cells are required for transfer of contact sensitivity (CS). This study confirms that day 4 immune cells depleted of gammadelta cells fail to transfer CS to trinitrochlorobenzene (TNP-Cl) systemically and demonstrates that administration of anti-gammadelta mo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270131
更新日期:1997-01-01 00:00:00
abstract::Mice with homologous disruption of the gene coding for either the p35 subunit or the p40 subunit of interleukin-12 (IL-12) and derived from a strain genetically resistant to infection with Leishmania major have been used to study further the role of this cytokine in resistance to infection and the differentiation of f...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260722
更新日期:1996-07-01 00:00:00
abstract::Mercury-induced autoimmune glomerulonephritis in the Brown-Norway (BN) rat is characterized by the successive appearance of linear and granular glomerular IgG deposits. Anti-laminin autoantibodies represent the major part of the anti-glomerular basement membrane (GBM) antibodies produced in this model. Fusions were pe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200114
更新日期:1990-01-01 00:00:00
abstract::In humans and in BALB/c mice, immune responses to the hormone insulin use evolutionarily related VHV (human) and VHIX (murine) gene families. To determine if these structural relationships include regulatory elements, BALB/c mice were pretreated with autologous immunoglobulin G (IgG) monoclonal antibodies (mAb) that r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220938
更新日期:1992-09-01 00:00:00
abstract::Allergic asthma is a chronic inflammation of the airways mediated by an adaptive type 2 immune response. Upon allergen exposure, group 2 innate lymphoid cells (ILC2s) can be rapidly activated and represent an early innate source of IL-5 and IL-13. Here, we used a house dust mite (HDM)-driven asthma mouse model to stud...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546119
更新日期:2016-06-01 00:00:00
abstract::Membrane regulatory molecules normally prevent complement activation by autologous cells, therefore we compared the membrane control system of human lymphoid cell lines which activate or not human complement through the alternative pathway (AP). Membrane expression of decay-accelerating factor (DAF), membrane cofactor...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201218
更新日期:1990-12-01 00:00:00
abstract::A previously designed isotopic in vivo method for measuring delayed hypersensitivity was used with nonantigenic (or weakly antigenic) phlogogenic substances. A strong reaction was observed in some circumstances, witnessing the fact that the method has a specific and a nonspecific component. The reaction is drastically...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060606
更新日期:1976-06-01 00:00:00
abstract::In the bone marrow, the passage of developing B cells through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636569
更新日期:2007-04-01 00:00:00
abstract::B cells in rabbit preferentially utilize the 3'-most VH gene, VH1, in VDJ genes. To determine whether the preferential utilization of VH1 results from preferential rearrangement, we examined VH gene usage in nonproductive VDJ gene rearrangements, genes that would not be influenced by antigen selection. Since nonproduc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250927
更新日期:1995-09-01 00:00:00
abstract::Graft-versus-host disease (GVHD) caused by donor T cells attacking recipient tissues is a major cause of morbidity and mortality following allogeneic hematopoietic stem cell transplantation (alloSCT). Studies have shown that effector memory T (T(EM) ) cells do not cause GVHD but are capable of immune functions post-tr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141678
更新日期:2011-09-01 00:00:00
abstract::LL-37, derived from human cathelicidin, stimulates immune responses in neutrophils. Although FPR2 and P2X7 were proposed as LL-37 receptors, we have shown that among 21 neutrophil receptors only CXCR2 was down-regulated by LL-37. LL-37 functions similarly to CXCR2-specific chemokines CXCL1 and CXCL7 in terms of recept...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939496
更新日期:2009-11-01 00:00:00
abstract::This article describes the study of the functional relationship between auto-tumor-reactive CD4(+) T cell clones (TCC) and autologous malignant B cells. Four auto-tumor-reactive CD4(+) TCC were derived from tumor-infiltrating T lymphocytes (TIL-T) from a freshly isolated human follicular lymphoma by the following tech...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199910)29:10<3188::AID-IMM
更新日期:1999-10-01 00:00:00
abstract::Growing evidence suggests that some immune responses are mediated not only by conventional and distinct NK cells and CTL, but also by T cell subsets expressing NK receptors and NK cell-associated molecules. Consistent with our previously published finding that the mAb 1F8 identifies non-T/non-B cells in Xenopus that e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1574::AID-IMMU1574>3
更新日期:2002-06-01 00:00:00
abstract::Protein antigens with both allergenic and immunoprotective properties represent appropriate molecules to study IgE and IgG regulation. We have established a panel of T cell clones specific to bee venom phospholipase A2 (PLA) from human individuals allergic, hyposensitized or immune (protected) to bee sting. All clones...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220605
更新日期:1992-06-01 00:00:00
abstract::CBA/J mice immunized with thyroglobulin (Tg) develop an experimental autoimmune thyroiditis (EAT) with lymphocytic infiltration of the thyroid glands, autoantibodies to Tg and occurrence of EAT-specific T cells. When these mice were treated for 4 weeks after immunization with 1 mg/week of a monoclonal antibody (mAb) t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230143
更新日期:1993-01-01 00:00:00
abstract::A monoclonal antibody (12-15) reactive with the mouse CD2 was used to study the expression of the antigen in different lymphoid cell subsets. By two-color immunofluorescence using B or T cell-specific reagents and cell sorting in combination with biochemical analysis we provide evidence that the CD2 antigen is present...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190826
更新日期:1989-08-01 00:00:00