Abstract:
:B cells in rabbit preferentially utilize the 3'-most VH gene, VH1, in VDJ genes. To determine whether the preferential utilization of VH1 results from preferential rearrangement, we examined VH gene usage in nonproductive VDJ gene rearrangements, genes that would not be influenced by antigen selection. Since nonproductive VDJ gene rearrangements are found infrequently in neonatal and adult rabbits, we examined the VDJ gene rearrangements during early fetal development. According to nested-primer polymerase chain reaction analyses, VDJ genes in fetal liver appeared around day 14 of gestation and nucleotide sequence analyses of VDJ genes cloned from 14- to 28-day-old fetuses showed that many of the genes were nonproductive. We found that most of these nonproductive genes had utilized VH1 indicating to us that the preferential utilization of VH1 is due to preferential rearrangement. We compared the nucleotide sequence of the promoter region of VH1 with sequences of several other unutilized and infrequently utilized VH genes and found that the core transcriptional factor motifs were similar in all VH promoters examined. We suggest that transcriptional activity of the VH1 promoter region does not explain the preferential rearrangement of VH1.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Tunyaplin C,Knight KLdoi
10.1002/eji.1830250927subject
Has Abstractpub_date
1995-09-01 00:00:00pages
2583-7issue
9eissn
0014-2980issn
1521-4141journal_volume
25pub_type
杂志文章abstract::Cartilage-specific proteins are considered potential autoantigens that could continuously fuel autoimmune responses directed to the joints in rheumatoid arthritis (RA). Using recombinant chimeric collagen type II we have identified one major type II collagen (CII) epitope (denoted U1) recognized by RA sera. The U1 epi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200106)31:6<1666::aid-immu1666>3
更新日期:2001-06-01 00:00:00
abstract::Cells from the monoclonal B cell lymphoma I.29 expressing surface IgM (mu +) are capable of differentiating in vitro to IgM secretion and of switching to IgA or IgE production in response to lipopolysaccharide (LPS) stimulation. To determine whether a single mu + B cell is capable of undertaking both differentiative p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170419
更新日期:1987-04-01 00:00:00
abstract::The mechanisms responsible for the down-modulation of the activation of separated CD4(+) or CD8(+) human T cell blasts were studied using cells obtained from healthy donors. In the presence of IL-2, human CD8(+) T cell blasts were more sensitive than CD4(+) T cell blasts to regulation by APO2 ligand/TNF-related apopto...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526046
更新日期:2005-06-01 00:00:00
abstract::Antigen-specific human T lymphocytes were selected by adsorption of peripheral blood mononuclear cells on monolayers of antigen-treated autologous monocytes. The enriched cell populations were propagated in interleukin 2-containing medium for 60-80 days. Their activity was tested by a proliferative response against th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131116
更新日期:1983-11-01 00:00:00
abstract::Mast cells are important cellular constituents of epithelial-mesenchymal interactions, densely located at sites of microbial entry into the host where they are continuously exposed to products from commensals. In order to avoid excessive activation and the associated pathology, mast cell responses to TLR agonists must...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242786
更新日期:2013-04-01 00:00:00
abstract::Mucosal boosting of BCG-immunised individuals with a subunit tuberculosis (TB) vaccine would be highly desirable, considering that the lungs are the principal port of entry for Mycobacterium tuberculosis (MTB) and the site of the primary infection and reactivation. However, the main roadblock for subunit TB vaccine de...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343887
更新日期:2014-02-01 00:00:00
abstract::Natural interferon-producing cells (NIPC), also called plasmacytoid dendritic cells, are the most potent producers of IFN-alpha in response to viral and bacterial components, serving an important function in innate immune defences. The present work demonstrates that NIPC responsiveness can be primed by immunisation, i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200525998
更新日期:2005-08-01 00:00:00
abstract::The magnitude of the IgE antibody response against the phosphorylcholine (PC) hapten and the rate of age-related change of IgE formation is polymorphic in the three long-lived and six relatively short-lived strains of mice studied. Long-lived mice showed an early increase, followed by a decline in the levels of anti-P...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160621
更新日期:1986-06-01 00:00:00
abstract::Tuberculosis (TB) is one of the world's most pernicious diseases mainly due to immune evasion strategies displayed by its causative agent Mycobacterium tuberculosis (Mtb). Blood monocytes (Mos) represent an important source of DCs during chronic infections; consequently, the alteration of their differentiation constit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242557
更新日期:2013-02-01 00:00:00
abstract::In contrast to previous studies, we report that heterologous anti-delta antibodies can act as a powerful multi-class humoral immunosuppressant. Primary direct plaque-forming cell (IgM) responses of BALB/c mice injected from birth with a rabbit anti-delta antiserum were reduced to 3% of control levels against a T-depen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160816
更新日期:1986-08-01 00:00:00
abstract::We studied the competitive repopulation by different B cells of irradiated mice reconstituted with bone marrow from either congenic or Ig-transgenic (TG) mice mixed at different ratios. We found that after reconstitution, the number of B cells recovered in the different chimeras is similar and independent of the ratio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250636
更新日期:1995-06-01 00:00:00
abstract::We have fractionated human tonsillar B cells on the basis of CD5 expression and determined the nucleotide sequences of immunoglobin light chain variable (V) regions encoded by the single member of the V chi 4 gene family in both CD5+ and CD5- populations. The majority of cDNA from both CD5+ and CD5- B cells population...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230636
更新日期:1993-06-01 00:00:00
abstract::Immunologic control of C3 gene expression by tissue macrophages can be accomplished by treatment of spleen fragments with anti-C3 antibody. We now demonstrate that suppression of C3 requires participation of T lymphocytes of both the CD4+ and CD8+ phenotypes. Pretreatment of splenic tissue with anti-Thy-1.2 monoclonal...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221212
更新日期:1992-12-01 00:00:00
abstract::NKp46 is a novel triggering receptor expressed by all human NK cells that is involved in natural cytotoxicity. In this study we show that the surface density of NKp46 may vary in different NK cells and that a precise correlation exists between the NKp46 phenotype of NK clones and their natural cytotoxicity against HLA...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199905)29:05<1656::AID-IMM
更新日期:1999-05-01 00:00:00
abstract::A multiparametric analysis of the resident immune populations in the parotid, submandibular and sublingual salivary glands was done in single-cell suspensions. The incidence of T and B cells and of macrophages was assessed using phenotypic markers in immunofluorescent staining and a functional assay was used to enumer...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160313
更新日期:1986-03-01 00:00:00
abstract::Co-culture of purified T and B cells obtained from cytochrome c-specific TCR- and hen egg lysozyme (HEL)-specific Ig-transgenic mice was used to examine the role of B cell receptor (BCR) ligation and TCR affinity on the efficiency of T-B cell collaboration. The results showed that BCR ligation of naive B cells with HE...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199812)28:12<4037::AID-IMM
更新日期:1998-12-01 00:00:00
abstract::We have developed a long-term culture system using the murine bone marrow stromal cells MS-5 to support the growth of progenitor B cells with CD34-, CD10+, CD19+, and cytoplasmic mu chain (C mu)-negative surface phenotype from human CD34+ cells purified from umbilical cord blood (CB). When 10(3) CD34+ cells/well were ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<855::AID-IMMU
更新日期:1998-03-01 00:00:00
abstract::The mer receptor tyrosine kinase mediates phagocytosis of apoptotic cells and modulates cytokine production; it is also required for prevention of systemic autoimmune disease. Using a mer-specific antibody, we have confirmed the presence of mer on macrophages and now report its expression on NK cells, NKT cells, and d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324076
更新日期:2003-08-01 00:00:00
abstract::Just over a decade ago, it was established that NK cells in the thymus do not follow precisely the same developmental pathway as conventional NK cells that develop in the bone marrow. Subsequently, it has emerged that NK cells are one branch of a family of innate lymphoid cells (ILCs). ILC1s and thymic NK cells have, ...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201747038
更新日期:2017-05-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) and natural killer (NK) cells elaborate a cytolytic protein named perforin or cytolysin. It was widely held that, in vivo, high quantities of perforin are not present in resting lymphocytes and are usually produced only by activated lymphocytes found under pathological conditions. Until n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230907
更新日期:1993-09-01 00:00:00
abstract::The influence of Th2 cytokines in tuberculosis has been a matter of dispute. Here we report that IL-4 has a profound regulatory effect on the infection of BALB/c mice with Mycobacterium tuberculosis. Depletion of IL-4 with a neutralizing mAb caused only evanescent reduction of lung infection, but when combined with i....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636764
更新日期:2007-03-01 00:00:00
abstract::Mycoplasma arthritidis produces an as yet undefined soluble molecule (MAS) that has a potent mitogenic effect on T cells of several species. We have used cloned human cytotoxic and proliferative T lymphocytes to dissect the molecular mechanism of T cell activation by this mitogen. Reactivity to MAS is clonally express...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181112
更新日期:1988-11-01 00:00:00
abstract::Cytomegaloviruses (CMVs) are highly prevalent, persistent human pathogens that not only evade but also shape our immune responses. Natural killer (NK) cells play an important role in the control of CMV and CMVs have in turn developed a plethora of immunoevasion mechanisms targeting NK cells. This complex interplay can...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201646762
更新日期:2018-01-01 00:00:00
abstract::The human T lymphocyte-specific CD2 (T11) molecule, that regulates T cell activation, is capable of mediating increases of intracellular cAMP. Monoclonal antibodies directed to different epitopes of the CD2 molecule which either induce or inhibit T cell proliferation are capable of triggering an increase of cAMP compa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180620
更新日期:1988-06-01 00:00:00
abstract::The B cell line 721.174 has lost the ability to present intracellular antigens to major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTL). This phenotype results from a homozygous deletion in the MHC that includes the peptide transporter genes TAP1 and TAP2, and the proteasome subunits ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250238
更新日期:1995-02-01 00:00:00
abstract::Disseminated infections by the opportunistic yeast Cryptococcus neoformans are characterized by accumulation in tissues of glucuronoxylomannan (GXM), the major component of the capsular polysaccharide. We investigated binding, uptake, and disposal of GXM by peripheral blood neutrophils and monocytes, and the effect of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323388
更新日期:2003-04-01 00:00:00
abstract::Langerhans cells (LC) are the principal dendritic cell (DC) population in the epidermis of the skin. Owing to their prominent position at the environmental barrier, LC have long been considered to be prototypic sentinel DC. More recently, the precise role of LC in the initiation and control of cutaneous immune respons...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200838397
更新日期:2008-09-01 00:00:00
abstract::Cholera toxin (CT) can function as a potent adjuvant in the mucosal immune response. However, we have found that treatment of A20-HL murine B lymphoma cells with CT severely inhibits the presentation of ovalbumin (OVA) to cells of the T cell clone 42-6A specific for OVA(323-339)/I-Ad, whereas it does not affect the pr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199905)29:05<1561::AID-IMM
更新日期:1999-05-01 00:00:00
abstract::In this investigation we compare the repopulation of the CD8+ T cell compartments of bone marrow (BM) chimeras by either normal nontransgenic or T cell receptor (TcR) alpha beta-transgenic (TG) CD8+ T cells, the fate of TG and non-TG CD8+ T cells in different parabionts and the survival of TG and non-TG peripheral CD8...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261115
更新日期:1996-11-01 00:00:00
abstract::Tissue macrophages and their precursors-the blood monocytes-respond rapidly to a bacterial infection with the release of inflammatory mediators. These mediators are involved in the recruitment of phagocytic cells, principally neutrophils, from the blood to the site of infection. To initiate this process macrophages an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250545
更新日期:1995-05-01 00:00:00