Abstract:
:Reactive oxygen species (ROS) are implicated in the modulation of diverse processes including immune responses. To evaluate the effects of metabolic ROS produced by mitochondria on B-cell function and development, we created transgenic (Tg) mice expressing a phosphorylation-defective mutant of succinate dehydrogenase A in B cells (bSDHAY215F ). Splenic B cells in male, but not female, bSDHAY215F mice produced three times more ROS than those in the control mice, and had decreased production of IgM, IgG1 , and IgG3 , and affinity maturation of IgG1 against T-cell-dependent antigens. Following immunization, the male bSDHAY215F mice further displayed suppressed germinal center (GC) formation, and proliferation of GC B cells. Signaling analysis revealed defects in the intrinsic BCR responses, such as activation of Lyn, Btk, and PLCγ2, thus resulting in reduced intracellular Ca2+ mobilization. Notably, the expression levels of B-cell co-receptor CD19 and its interaction with Lyn after BCR ligation were significantly reduced in B cells from male bSDHAY215F mice. These results suggest that mitochondrial ROS suppress humoral immune responses through reduction of CD19 expression and resultant BCR signaling in B cells. Therefore, B-cell immunity may be more labile to oxidative stress in male mice than in female mice.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Ogura M,Inoue T,Yamaki J,Homma MK,Kurosaki T,Homma Ydoi
10.1002/eji.201646342subject
Has Abstractpub_date
2017-02-01 00:00:00pages
406-418issue
2eissn
0014-2980issn
1521-4141journal_volume
47pub_type
杂志文章abstract::The zinc-finger transcription factor GATA3 serves as a master regulator of T-helper-2 (Th2) differentiation by inducing expression of the Th2 cytokines IL-4, IL-5 and IL-13 and by suppressing Th1 development. Here, we investigated how GATA3 affects Th17 differentiation, using transgenic mice with enforced GATA3 expres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737840
更新日期:2008-09-01 00:00:00
abstract::Twelve continuous rat tissue cultured mast cell (MC) lines were established by prolonged culture of rat peritoneal MC in the absence of added factors or feeder layers. Two of these lines, RCMC1 and RCMC2, have been briefly described previously, seven others are now also described. Both RCMC1 and RCMC2 lack a marker ch...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200127
更新日期:1990-01-01 00:00:00
abstract::In the present study, interleukin 1 (IL 1)-containing media from different sources, namely a murine macrophage cell line (P388D1), rabbit peritoneal macrophages, and human peripheral blood mononuclear cells, were compared for their effect on thymocyte proliferation and on collagenase and PGE2 secretion by chondrocytes...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140603
更新日期:1984-06-01 00:00:00
abstract::Deficiencies in terminal complement components, including the component C7, are uncommon and associated with an increased risk of recurrent systemic neisserial infection. A total of 22 molecular defects have been reported in the C7 gene with both complete (C7Q0) and subtotal (C7SD) C7 deficiencies. In this study we re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636812
更新日期:2007-05-01 00:00:00
abstract::Recognition of foreign DNA by cytosolic innate immune receptors triggers the production of IFN-beta. However, it is unclear whether different types of DNA ligands are recognized by similar receptors and whether the resulting response is distinct from the endosomal TLR response. To address these questions, we compared ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939344
更新日期:2009-07-01 00:00:00
abstract::The second HLA-DR molecules, which are encoded by loci different from HLA-DRB1 are weakly polymorphic. Predominant alleles such as HLA-DRB3*0101, HLA-DRB4*0101 and HLA-DRB5*0101 are therefore interesting targets to define antigenic peptides with major impact for the entire population. Strikingly, they have been poorly...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200106)31:6<1837::aid-immu1837>3
更新日期:2001-06-01 00:00:00
abstract::We have developed a quantitative assay for the measurement of class I assembly induced by peptide. We have applied this assay to H-2Db, Kb and HLA-A2.1 with a panel of 49 overlapping peptides derived from HIV-1 gag protein. We find that the effects of peptide on assembly form a continuous distribution. By defining pos...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210909
更新日期:1991-09-01 00:00:00
abstract::CD30 is a costimulatory molecule of the TNF receptor superfamily, expressed on activated T and B cells. Previously, we have shown in a murine asthma model the crucial role of CD30 signaling for the development of this Th2-cell-mediated disease. In the present study, we investigated the role of CD30 in the maintenance ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839004
更新日期:2009-07-01 00:00:00
abstract::Vaccination of mice with irradiated Schistosoma mansoni larvae confers high levels of immunity which is mediated by Th1-type lymphocytes. To investigate a possible role for IL-12 in the induction of protection, we have compared the immune response of IL-12 p40-deficient (KO) mice and their C57BL/6 (WT) counterparts fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2827::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::Membrane-coated microvesicles (MVs) have been identified as important mediators in intercellular communication. During the process of apoptosis, dying cells dynamically release MVs. Neutrophils are the most abundant type of leukocytes in the circulation. Due to their very short lifespan, it is likely that they are the...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546203
更新日期:2017-05-01 00:00:00
abstract::The in vitro recognition of acetylsalicilyl ovalbumin, penicilloylated bovine IgG (BPO-BGG) and the multichain copolymer poly-L-(Tyr, Glu)-poly-D L-Alapoly-L-Lys [(T, G)-A-L] by primed (2 x 13) F1 cells could be markedly and specifically blocked by alloantisera directed against "receptors" (R) or recognition structure...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830051012
更新日期:1975-10-01 00:00:00
abstract::Newborn mouse spleen, whose cells strongly suppress the in vitro humoral response of adult spleen cells, is essentially a hematopoietic organ. It contains a large percentage of proliferating cells, among which about 50% are erythroblasts (identified by their spectrin content) and about 15% are cells of the myelocytic ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110112
更新日期:1981-01-01 00:00:00
abstract::Dendritic cells (DC) play the dual task of initiating cellular immunity against potentially harmful foreign antigens (Ag), while maintaining immunological tolerance to self-Ag and environmental Ag. As an approach to induce Ag-specific suppression, we and others introduced CD95 ligand (L) cDNA into DC. The resulting "k...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<1035::AID-IMMU1035>3
更新日期:2002-04-01 00:00:00
abstract::The cytokine IL-7 and its receptor are essential for normal B and T lymphopoiesis. We have analyzed the role of this receptor in B cell development throughout ontogeny in IL-7 receptor alpha-deficient mice. We demonstrate that the IL-7 receptor becomes progressively more important with age. B lymphopoiesis takes place...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425217
更新日期:2004-12-01 00:00:00
abstract::Exosomes from APC are nano-vesicles that can induce antigen-specific T cell responses and are presently explored as therapeutic tools in different clinical settings. Investigations of the capacity of exosomes to stimulate T cells in vitro have mostly been performed on T cell hybridomas, clones or lines. Whether exosom...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535615
更新日期:2006-07-01 00:00:00
abstract::Major histocompatibility complex (MHC) class II molecules have been implicated in cell adhesion in two ways. In addition to the well-established role of class II antigens in low-affinity adhesion provided by interactions between class II and CD4, recent data indicated that class II may also induce adhesion between T a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210921
更新日期:1991-09-01 00:00:00
abstract::This study analyzes the role of the measles virus (MV) receptor, i.e. the human CD46 molecule, in the MHC class II-restricted presentation of MV hemagglutinin (H). We generated transgenic mice ubiquitously expressing CD46, with a similar level of transgene expression on the surface of antigen-presenting cells (APC), i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199804)28:04<1301::AID-IMM
更新日期:1998-04-01 00:00:00
abstract::The transforming protein of mouse polyomavirus, the mouse middle T antigen (MomT), and its counterpart in the hamster polyomavirus, the hamster middle T antigen (HamT), interact with a number of cellular proteins. Among these are members of the Src family of tyrosine kinases, the phosphatidylinositol 3-kinase, the ser...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250212
更新日期:1995-02-01 00:00:00
abstract::Bispecific antibodies binding to the TCR/CD3 complex and to a tumor-associated surface molecule can be used to target cytotoxic T lymphocytes against tumor cells. We reasoned that high-affinity binding to CD3 may reduce the efficiency of T cell stimulation and target the bispecific reagent to T cells rather than to tu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200211)32:11<3102::AID-IMMU3102>
更新日期:2002-11-01 00:00:00
abstract::Murine splenic T lymphocytes display maximal cellular myc gene (c-myc) expression already 3 h after concanavalin A stimulation and subsequent down-regulation before the onset of DNA synthesis. Stimulation by leucoagglutinin in the presence or absence of interleukin 2 leads to only low initial levels of c-myc-specific ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160319
更新日期:1986-03-01 00:00:00
abstract::Responding B cells in specific immune responses diversify their immunoglobulin genes and are selected on their variant antigen receptors in the microenvironment of the germinal center. The patterns of mutations previously reported for immunglobulin (Ig) genes have supported mechanistic hypotheses of either error-prone...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240814
更新日期:1994-08-01 00:00:00
abstract::Tetanus toxin has been a valuable model antigen to study the MHC class II-restricted antigen processing pathway and is also frequently used to provide T helper determinants in vaccine formulations. To date most basic studies on the processing of this antigen have utilized human T and B cell clones. As a first step tow...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200202)32:2<530::AID-IMMU530>3.0
更新日期:2002-02-01 00:00:00
abstract::Mouse peritoneal macrophages were charged with IgG molecules in monomeric (mIgG), heat-aggregated (agIgG) or antigen-complexed (acIgG) form. Upon exposure to 37 degrees C, all bound IgG ligand types are redistributed on the cell surface due to the mobilization of their corresponding Fc receptor (FcR). The major findin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830091213
更新日期:1979-12-01 00:00:00
abstract::Dendritic cells (DCs) are essential in dictating the nature and effectiveness of immune responses. In the intestine DCs can be separated into discrete subsets, defined by expression of CD11b and CD103, each with different developmental requirements and distinct functional potential. Recent evidence has shown that diff...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747073
更新日期:2018-01-01 00:00:00
abstract::The major role of cells of the dendritic family in immunity and tolerance has been amply documented. Since their discovery in 1973, these cells have gained increasing interest from immunologists, as they are able to detect infectious agents, migrate to secondary lymphoid tissue, and prime naive T lymphocytes, thereby ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201242480
更新日期:2012-10-01 00:00:00
abstract::Antigenic peptides have been found associated with heat shock proteins (HSP) including cytoplasmic HSP70 and heat shock cognate protein 70 as well as the endoplasmic reticulum-resident glucose-regulated protein 94. Recently, HSP70 transfection has been reported to increase MHC class I cell surface expression and antig...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<3925::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::The costimulatory receptor OX40 is expressed on activated T cells and regulates T-cell responses. Here, we show the efficacy and mechanism of action of an OX40 blocking antibody using the chronic house dust mite (HDM) mouse model of lung inflammation and in vitro HDM stimulation of cells from HDM allergic human donors...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445163
更新日期:2015-04-01 00:00:00
abstract::Transgenic CBA (H-2k haplotype) mice expressing the H-2 Kb major histocompatibility complex (MHC) class I gene under control of transcriptional promoter elements from a milk protein gene display high-level H-2 Kb transcription in lactating mammary glands and low-level transcription in skin and thymus of male and virgi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221027
更新日期:1992-10-01 00:00:00
abstract::Pathogens have developed a plethora of strategies to undermine host immune defenses in order to guarantee their survival. For large DNA viruses, these immune evasion mechanisms frequently rely on the expression of genes acquired from host genomes. Horizontally transferred genes include members of the immunoglobulin su...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201746984
更新日期:2017-05-01 00:00:00
abstract::Gout manifests as recurrent episodes of acute joint inflammation and pain due to the deposition of monosodium urate (MSU) crystals within the affected tissue in a process dependent on NLRP3 inflammasome activation. The synthesis, activation, and release of IL-1β are crucial for MSU-induced inflammation. The current st...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545798
更新日期:2016-01-01 00:00:00