Abstract:
:The interaction between T cell receptors (TCR) and peptide-major histocompatibility complex (pMHC) antigens can lead to varying degrees of agonism (T cell activation), or antagonism. The P14 TCR recognises the lymphocytic choriomeningitis virus (LCMV)-derived peptide, gp33 residues 33-41 (KAVYNFATC), presented in the context of H-2D(b). The cellular responses to various related H-2D(b) peptide ligands are very well characterised, and P14 TCR-transgenic mice have been used extensively in models of virus infection, autoimmunity and tumour rejection. Here, we analyse the binding of the P14 soluble TCR to a broad panel of related H-2D(b)-peptide complexes by surface plasmon resonance, and compare this with their diverse cellular responses. P14 TCR binds H-2D(b)-gp33 with a KD of 3 microM (+/-0.5 microM), typical of an immunodominant antiviral TCR, but with unusually fast kinetics (k(off) = 1 s(-1)), corresponding to a half-life of 0.7 s at 25 degrees C, outside the range previously observed for murine agonist TCR/pMHC interactions. The most striking feature of these data is that a very short half-life does not preclude the ability of a TCR/pMHC interaction to induce antiviral immunity, autoimmune disease and tumour rejection.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Boulter JM,Schmitz N,Sewell AK,Godkin AJ,Bachmann MF,Gallimore AMdoi
10.1002/eji.200636743subject
Has Abstractpub_date
2007-03-01 00:00:00pages
798-806issue
3eissn
0014-2980issn
1521-4141journal_volume
37pub_type
杂志文章abstract::The transferrin receptor (TfR) was recently proposed as putative natural killer (NK) cell target structure. Here data are presented against this hypothesis and it is shown that low TfR expression and high NK sensitivity can occur concommitantly . K562 cells were studied at various stages of cell proliferation. No chan...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140412
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abstract::The killer cell lectin-like receptor G1 (KLRG1) is an inhibitory receptor expressed by memory T cells and NK cells in man and mice. It is frequently used as a cell differentiation marker and members of the cadherin family are ligands for KLRG1. The present study provides new insights into the interaction of mouse KLRG...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
abstract::Protein kinase C (PKC) has been implicated in the signaling of a number of cellular responses including activation of T cells. In the present report we have evaluated the effect of increased cAMP levels on PKC activation after stimulation of two distinct receptor systems on normal human T cells. PKC substrate phosphor...
journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
abstract::The level of cyclic AMP (cAMP) in human lymphocytes in the presence or absence of isoproterenol stimulation was studied in various lymphocyte subpopulations containing different proportions of B and T lymphocytes. Lymphocytes from thymus and peripheral blood (which contain a majority of T cells) were more sensitive to...
journal_title:European journal of immunology
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abstract::The essential role of the transcription factor Foxp3 in the development and function of Treg has been well documented. The role of Foxp3 in non-Treg, however, is not fully understood. Emerging evidence indicates that Foxp3 expression is not confined to CD4+CD25+ Treg. The present study shows that in Foxp3 transgenic (...
journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:2001-02-01 00:00:00
abstract::Allogeneic bone marrow transplantation in the neonate is an effective way of inducing permanent tolerance to donor tissue. To do the same in the immunocompetent adult requires immunosuppression to counter host-versus-graft alloreactivity. Conditioning with monoclonal antibodies (mAb) to CD4 and CD8 has been sufficient...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221111
更新日期:1992-11-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2001-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
abstract::To determine the molecular mechanisms of T cell stimulation by staphylococcal enterotoxin A (SEA), we examined the expression of T cell receptor (TcR) V beta on the T cells from four strains of mice stimulated in vitro with SEA, using flow cytometric analysis for the number of T cells bearing V beta 3, V beta 6, V bet...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200323
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abstract::It is well established that therapeutic impairment of Foxp3+ Treg in mice and humans favors immune rejection of solid tumors. Less explored is the impact Foxp3 allelic variants may have on tumor incidence, progression and therapy. In this work, we tested and demonstrate that the Foxp3fgfp reporter allele, found previo...
journal_title:European journal of immunology
pub_type: 杂志文章
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abstract::Crosslinking of Fc γ receptor II B (FcγRIIB) and the BCR by immune complexes (IC) can downregulate antigen-specific B-cell responses. Accordingly, FcγRIIB deficiencies have been associated with B-cell hyperactivity in patients with systemic lupus erythematosus and mouse models of lupus. However, we have previously sho...
journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
pub_type: 杂志文章,收录出版
doi:10.1002/eji.1830131207
更新日期:1983-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270818
更新日期:1997-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
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更新日期:2012-04-01 00:00:00
abstract::Dendritic cells expanded from mouse bone marrow (BMDC) with granulocyte/macrophage-colony-stimulating factor have potent T cell-stimulatory properties both in vitro and in vivo. This has been well documented for major histocompatibility complex (MHC) class II-restricted responses, and more recently using peptide-loade...
journal_title:European journal of immunology
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doi:10.1002/eji.1830270141
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180620
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324053
更新日期:2004-01-01 00:00:00
abstract::In this study we have investigated the specificity of bioassays in which interleukin (IL) 1 and/or IL 6 are active. The thymocyte assay cannot be used to discriminate between IL 1 and IL 6; both monokines are active in this assay. Moreover the detection limit for both IL 1 and IL 6 is around 100 pg/ml. IL 6 activity c...
journal_title:European journal of immunology
pub_type: 杂志文章
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abstract::B1 cells are a subset of B lymphocytes found in many species and are implicated in the development of autoimmunity. B1 cells have previously been shown to be suppressed by the T helper (Th)1 cytokine interferon (IFN)-gamma, and to be stimulated by the Th2 cytokines interleukin (IL)-2, IL-4, IL-5 and IL-10. To examine ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260134
更新日期:1996-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250114
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journal_title:European journal of immunology
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abstract::Interleukin-5 (IL-5) plays a critical role in the pathogenesis of eosinophil-associated allergic disorders, such as asthma. IL-5 may also play a major role in the development of eosinophilia-associated lymphoproliferative disorders caused by human T lymphotropic virus type I (HTLV-I). In this study, we have investigat...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
abstract::Dendritic cells (DC) were cultured from mouse bone marrow (BM) progenitors in low concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) (GM(lo) DC) by two different protocols. The phenotype and functional properties of these GM(lo) DC were compared to those of standard BM-DC cultures generated in...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2000-07-01 00:00:00
abstract::Reciprocal induction of the Th1 and Th17 immune responses is essential for optimal protection against Mycobacterium tuberculosis (Mtb); however, only a few Mtb antigens are known to fulfill this task. A functional role for resuscitation-promoting factor (Rpf) E, a latency-associated member of the Rpf family, in promot...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445329
更新日期:2015-07-01 00:00:00