CD11c provides an effective immunotarget for the generation of both CD4 and CD8 T cell responses.

Abstract:

:The magnitude and quality of T cell responses generated when Ag is targeted to receptors on DC is influenced by both the specific receptor targeted and its distribution among DC subsets. Here we examine the targeting of the model Ag OVA to potential DC targets, including CD11c, CD205, MHC class II, CD40, TLR2 and FcgammaRII/III, using a panel of (Fab' x OVA) conjugates. In vitro studies identified CD11c, CD205 and MHC class II as superior and comparably effective immunotargets for the delivery of OVA to APC for presentation to T cells. In vivo studies, however, showed a marked advantage of targeting Ag to CD11c for both CD4 (OT-II) and CD8 (OT-I) responses, with robust stimulation after a single, low dose (equivalent to 0.5 microg OVA); in contrast, (anti-CD205 x OVA) and (anti-MHC class II x OVA) resulted in markedly less proliferation of both OT-I and OT-II cells. Biodistribution and immunohistochemical studies suggest that the exceptional ability of CD11c to capture Ag in lymphoid tissues may, at least partially, explain its ability to promote T cell responses. These results suggest that targeting antigen via CD11c offers a previously unappreciated strategy for vaccine development which, unlike most targets, delivers robust responses of both CD4 and CD8 T cells.

journal_name

Eur J Immunol

authors

Castro FV,Tutt AL,White AL,Teeling JL,James S,French RR,Glennie MJ

doi

10.1002/eji.200838302

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

2263-73

issue

8

eissn

0014-2980

issn

1521-4141

journal_volume

38

pub_type

杂志文章
  • DNA fusion gene vaccines induce cytotoxic T-cell attack on naturally processed peptides of human prostate-specific membrane antigen.

    abstract::For long-term attack on tumor cells in patients with prostate cancer, induction of cytolytic T cells is desirable. Several lineage-specific target proteins are known and algorithms have identified candidate MHC class I-binding peptides, particularly for HLA-A*0201. We have designed tolerance-breaking DNA fusion vaccin...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201141518

    authors: Vittes GE,Harden EL,Ottensmeier CH,Rice J,Stevenson FK

    更新日期:2011-08-01 00:00:00

  • Cyclosporin A inhibits the delayed-type hypersensitivity reaction: impaired production of early pro-inflammatory mediator(s).

    abstract::The effect of cyclosporin A (CsA) on the delayed-type hypersensitivity (DTH) elicited in mice by sheep red blood cells was investigated. Evidence is presented that a single injection of CsA adversely affects the inflammatory reaction. Sensitized T lymphocytes initiate the DTH reaction by their recruiting activity on p...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830140407

    authors: Shidani B,Milon G,Marchal G,Truffa-Bachi P

    更新日期:1984-04-01 00:00:00

  • Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody.

    abstract::A monoclonal antibody, B20.1, was generated by fusing spleen cells from a Lou rat immunized with a soluble alpha/beta T cell receptor (TcR; V alpha 2/V beta 2) to mouse myeloma cells. Analysis of a panel of V alpha 2 mRNA-expressing T cell lines, hybridomas and transfectants revealed that the B20.1 antibody was specif...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830220217

    authors: Pircher H,Rebaï N,Groettrup M,Grégoire C,Speiser DE,Happ MP,Palmer E,Zinkernagel RM,Hengartner H,Malissen B

    更新日期:1992-02-01 00:00:00

  • Functional analysis of avian class I (BFIV) glycoproteins by epitope tagging and mutagenesis in vitro.

    abstract::Similarities between the physical structures of avian and mammalian major histocompatibility complex (MHC) class I glycoproteins have been proposed based on comparative alignment of their amino acid sequences. To investigate the physical structure of the chicken class I glycoprotein, we cloned the cDNA representing th...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830250740

    authors: Fulton JE,Thacker EL,Bacon LD,Hunt HD

    更新日期:1995-07-01 00:00:00

  • CD8(+) T cells regulate immune responses in a murine model of allergen-induced sensitization and airway inflammation.

    abstract::The role of CD8(+) T cells in the development of allergic airway disease is controversial. On the one hand, CD8(+) T cells are known to inhibit the development of airway hyperreactivity (AHR) in murine models of asthma. In humans, IL-10-producing CD8(+) T cells were shown to act as regulatory cells, inhibiting both pr...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200324623

    authors: Stock P,Kallinich T,Akbari O,Quarcoo D,Gerhold K,Wahn U,Umetsu DT,Hamelmann E

    更新日期:2004-07-01 00:00:00

  • The threshold for autoimmune T cell killing is influenced by B7-1.

    abstract::The concept that naive CD4+ and CD8+ T cells require co-stimulatory signals for activation and proliferation is well documented. Less clear is the need for co-stimulation during the effector phase of the T cell response. Here we examined the influence of B7-1 (CD80) during the effector phase of an autoimmune response ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(199803)28:03<949::AID-IMMU

    authors: Allison J,Stephens LA,Kay TW,Kurts C,Heath WR,Miller JF,Krummel MF

    更新日期:1998-03-01 00:00:00

  • A new method of radioiodine labeling of polyanionic macromolecules for radioimmunoassay.

    abstract::A method is described for radiolabeling polyanionic macromolecules by the formation of an electrostatic complex with a radioiodinated tyramine conjugated DEAE-dextran. Data is presented on the use of this technique for the detection and quantitation of immunospecific binding of polynitrophenylated proteins, deoxyribon...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830050604

    authors: Little JR,Blanke TJ,Little KD

    更新日期:1976-06-01 00:00:00

  • Maintenance of memory CD8 T cells: Divided over division.

    abstract::Once generated during an infection, memory CD8+ T cells can provide long-lasting protection against reinfection with an intracellular pathogen, but the longevity of this defense depends on the ability of these pathogen-specific memory cells to be maintained. It is generally believed that the bone marrow plays an impor...

    journal_title:European journal of immunology

    pub_type: 评论,杂志文章

    doi:10.1002/eji.201747249

    authors: Nolte MA,Goedhart M,Geginat J

    更新日期:2017-11-01 00:00:00

  • IL-23 leads to diabetes induction after subdiabetogenic treatment with multiple low doses of streptozotocin.

    abstract::IL-23, a proximal regulator of IL-17, may be a major driving force in the induction of autoimmune inflammation. We have used a model of subdiabetogenic treatment with multiple low doses of streptozotocin (MLD-STZ; 4 x 40 mg/kg body weight) in male C57BL/6 mice to study the effect of IL-23 on immune-mediated beta cell ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200535325

    authors: Mensah-Brown EP,Shahin A,Al-Shamisi M,Wei X,Lukic ML

    更新日期:2006-01-01 00:00:00

  • Negative regulation of autoreactive B cells in transgenic mice expressing a human pathogenic cold agglutinin.

    abstract::Cold agglutinins (CA) are autoantibodies that bind to erythrocyte carbohydrates at low temperatures and induce complement-mediated cell lysis, thus causing hemolytic anemia. Tolerance mechanisms towards CA-expressing B cells and the factors inducing pathogenic CA production are unknown. In order to develop an animal m...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(2000)30:8<2290::AID-IMMU2290>3.0

    authors: Havouis S,Dumas G,Avé P,Pritsch O,Huerre M,Dighiero G,Pourcel C

    更新日期:2000-08-01 00:00:00

  • Purified lymphocyte function-associated antigen-3 and T11 target structure are active in CD2-mediated T cell stimulation.

    abstract::In this study we have used cells expressing LFA-3 or T11TS, the human and sheep forms of the ligand of CD2, as well as the purified LFA-3 and T11TS molecules themselves to study their effects on T cell activation via the CD2-mediated "alternative pathway". Sheep red blood cells, which bind to CD2 via T11TS in E-rosett...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830171227

    authors: Tiefenthaler G,Hünig T,Dustin ML,Springer TA,Meuer SC

    更新日期:1987-12-01 00:00:00

  • The granzyme B inhibitor proteinase inhibitor 9 (PI9) is expressed by human mast cells.

    abstract::The activity of granzyme B, a main effector molecule of cytotoxic T lymphocytes (CTL) and natural killer cells, is regulated by the human intracellular serpin proteinase inhibitor 9 (PI9). This inhibitor is particularly expressed by CTL and dendritic cells, in which it serves to protect these cells against endogenous ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200425949

    authors: Bladergroen BA,Strik MC,Wolbink AM,Wouters D,Broekhuizen R,Kummer JA,Hack CE

    更新日期:2005-04-01 00:00:00

  • 5' and 3' untranslated regions contribute to the differential expression of specific HLA-A alleles.

    abstract::In hematopoietic stem cell transplantation (HSCT), when no HLA full-matched donor is available, alternative donors could include one HLA-mismatched donor. Recently, the low expressed HLA-C alleles have been identified as permissive mismatches for the best donor choice. Concerning HLA-A, the degree of variability of ex...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201545927

    authors: René C,Lozano C,Villalba M,Eliaou JF

    更新日期:2015-12-01 00:00:00

  • Interleukin-10 regulates TNF-alpha-converting enzyme (TACE/ADAM-17) involving a TIMP-3 dependent and independent mechanism.

    abstract::IL-10 is a potent anti-inflammatory molecule, which regulates TNF-alpha at multiple levels. We investigated whether IL-10 also modulated the activity of the TNF-alpha-converting enzyme (TACE). Using an ex vivo fluorogenic assay we observed that LPS rapidly induced TACE activity in monocytes coinciding with release of ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200737821

    authors: Brennan FM,Green P,Amjadi P,Robertshaw HJ,Alvarez-Iglesias M,Takata M

    更新日期:2008-04-01 00:00:00

  • Human dendritic cells activated with MV130 induce Th1, Th17 and IL-10 responses via RIPK2 and MyD88 signalling pathways.

    abstract::Recurrent respiratory tract infections (RRTIs) are the first leading cause of community- and nosocomial-acquired infections. Antibiotics remain the mainstay of treatment, enhancing the potential to develop antibiotic resistances. Therefore, the development of new alternative approaches to prevent and treat RRTIs is hi...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201747024

    authors: Cirauqui C,Benito-Villalvilla C,Sánchez-Ramón S,Sirvent S,Diez-Rivero CM,Conejero L,Brandi P,Hernández-Cillero L,Ochoa JL,Pérez-Villamil B,Sancho D,Subiza JL,Palomares O

    更新日期:2018-01-01 00:00:00

  • Role of epitope density in the induction of tolerance and immunity with thymus-independent antigens. III. Interaction of epitope density and receptor avidity.

    abstract::The induction of B cell tolerance to 2,4-dinitrophenyl conjugates of polysaccharide antigens (levan or dextran) was studied in mice primed with keyhole limpet hemocyanin (KLH) or 2,4,6-trinitrophenylated KLH. The relationship of the epitope density of the tolerogen with avidity of B cell receptors (as judged indirectl...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830060910

    authors: Desaymard C,Pearce B,Feldmann M

    更新日期:1976-09-01 00:00:00

  • Follicular regulatory T cells repress cytokine production by follicular helper T cells and optimize IgG responses in mice.

    abstract::Follicular helper T (Tfh) cells provide crucial help to germinal center B (GCB) cells for proper antibody production, and a specialized subset of regulatory T cells, follicular regulatory T (Tfr) cells, modulate this process. However, Tfr-cell function in the GC is not well understood. Here, we define Tfr cells as a C...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201546094

    authors: Wu H,Chen Y,Liu H,Xu LL,Teuscher P,Wang S,Lu S,Dent AL

    更新日期:2016-05-01 00:00:00

  • Aggravation of experimental cutaneous leishmaniasis in mice by administration of interleukin 3.

    abstract::Previous studies from our laboratory have shown that some in vitro maintained Leishmania major-specific L3T4+ T cells were capable of exacerbating cutaneous leishmaniasis after adoptive transfer to normal syngeneic mice. Results presented in this report show that these cells released substantial amounts of interleukin...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830180815

    authors: Feng ZY,Louis J,Kindler V,Pedrazzini T,Eliason JF,Behin R,Vassalli P

    更新日期:1988-08-01 00:00:00

  • Human CD8+ T cell blasts are more sensitive than CD4+ T cell blasts to regulation by APO2L/TRAIL.

    abstract::The mechanisms responsible for the down-modulation of the activation of separated CD4(+) or CD8(+) human T cell blasts were studied using cells obtained from healthy donors. In the presence of IL-2, human CD8(+) T cell blasts were more sensitive than CD4(+) T cell blasts to regulation by APO2 ligand/TNF-related apopto...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200526046

    authors: Bosque A,Pardo J,Martínez-Lorenzo MJ,Lasierra P,Larrad L,Marzo I,Naval J,Anel A

    更新日期:2005-06-01 00:00:00

  • Protein tyrosine kinase p59fyn is associated with the T cell receptor-CD3 complex in functional human lymphocytes.

    abstract::The binding of antigen to the multicomponent T cell antigen receptor (TcR)-CD3 complex leads to the activation of several signal transduction pathways which result in T lymphocyte proliferation and lymphokine secretion by molecular mechanisms and catalytic molecules as yet poorly defined. One of the earliest events th...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830220142

    authors: Gassmann M,Guttinger M,Amrein KE,Burn P

    更新日期:1992-01-01 00:00:00

  • Role of Ia. W39 in the interaction of antigen-presenting cells with T and B lymphocytes.

    abstract::Ia. W39 is a B cell differentiation antigen whose membrane expression is controlled by the xid gene. In this report we show that, analogous to its B cell expression, Ia. W39 is also present on a subset of Ia+ macrophages, indicating heterogeneity within that cell population. The Ir gene(s) for the antigenic determinan...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830120109

    authors: Huber BT,Rosenwasser LJ

    更新日期:1982-01-01 00:00:00

  • Cross-presentation of oral antigens by liver sinusoidal endothelial cells leads to CD8 T cell tolerance.

    abstract::After ingestion, oral antigens distribute systemically and provoke T cell stimulation outside the gastrointestinal tract. Within the liver, scavenger liver sinusoidal endothelial cells (LSEC) eliminate blood-borne antigens and induce T cell tolerance. Here we investigated whether LSEC contribute to oral tolerance. Ora...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200526034

    authors: Limmer A,Ohl J,Wingender G,Berg M,Jüngerkes F,Schumak B,Djandji D,Scholz K,Klevenz A,Hegenbarth S,Momburg F,Hämmerling GJ,Arnold B,Knolle PA

    更新日期:2005-10-01 00:00:00

  • Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists.

    abstract::Stimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists, or antagonists. Fo...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830271241

    authors: Bachmann MF,Mariathasan S,Bouchard D,Speiser DE,Ohashi PS

    更新日期:1997-12-01 00:00:00

  • Spontaneous apoptosis of dendritic cells is efficiently inhibited by TRAP (CD40-ligand) and TNF-alpha, but strongly enhanced by interleukin-10.

    abstract::In the lymphoid tissues, adaptive immune responses are initiated by the interaction of interdigitating dendritic cells (IDC) with naive T cells. To understand this interplay better, we used mature Langerhans cells (mLC), migrating from human epidermis, as the correlate of IDC ex vivo to evaluate the different effects ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830250722

    authors: Ludewig B,Graf D,Gelderblom HR,Becker Y,Kroczek RA,Pauli G

    更新日期:1995-07-01 00:00:00

  • Expression of major histocompatibility complex class I antigens at low levels in the thymus induces T cell tolerance via a non-deletional mechanism.

    abstract::Transgenic CBA (H-2k haplotype) mice expressing the H-2 Kb major histocompatibility complex (MHC) class I gene under control of transcriptional promoter elements from a milk protein gene display high-level H-2 Kb transcription in lactating mammary glands and low-level transcription in skin and thymus of male and virgi...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830221027

    authors: Husbands SD,Schönrich G,Arnold B,Chandler PR,Simpson E,Philpott KL,Tomlinson P,O'Reilly L,Cooke A,Mellor AL

    更新日期:1992-10-01 00:00:00

  • Cyclosporin A inhibits T cell receptor-induced interleukin-2 synthesis of human T lymphocytes by selectively preventing a transmembrane signal transduction pathway leading to sustained activation of a protein kinase C isoenzyme, protein kinase C-beta.

    abstract::Stimulation of human peripheral blood lymphocytes via T cell receptor/CD3 complex resulted in a bimodal activation of protein kinase(s) C (PKC). Within 10 min of stimulation PKC-alpha was translocated to, and thus activated in, the plasma membranes of human lymphocytes, followed by a fast dissociation of this isotype ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830231205

    authors: Szamel M,Bartels F,Resch K

    更新日期:1993-12-01 00:00:00

  • Genetic control of B cell function. III. IgVH-controlled polymorphism in the frequencies of B cells that recognize xenogeneic red blood cells.

    abstract::Previous work has shown that the primary IgM plaque-forming cell response of inbred mice to xenogeneic red blood cells (RBC) including sheep, horse and chicken RBC is under the control of two polymorphic genes or sets of genes, one linked to the Igh linkage group and the other of unknown linkage but unlinked to H-2 an...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830150205

    authors: Saizawa KM,Melchers I,Eichmann K

    更新日期:1985-02-01 00:00:00

  • Poxvirus semaphorin A39R inhibits phagocytosis by dendritic cells and neutrophils.

    abstract::The poxvirus A39R protein is a member of the semaphorin family that binds to Plexin C1, a molecule expressed on neutrophils and dendritic cells (DC). We previously showed that binding of A39R to Plexin C1 induces local rearrangement of the actin cytoskeleton and inhibits integrin-mediated adhesion, leading to cell ret...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200425669

    authors: Walzer T,Galibert L,De Smedt T

    更新日期:2005-02-01 00:00:00

  • Frequency, heterogeneity and encephalitogenicity of T cells specific for myelin oligodendrocyte glycoprotein in naive outbred primates.

    abstract::Auto-reactive T cells present in healthy subjects remain in a state of unresponsiveness, but may trigger autoimmunity under various situations. Although myelin oligodendrocyte glycoprotein (MOG) is a potential target antigen in multiple sclerosis (MS), MOG-reactive T cell responses are present in the blood of both hea...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(2001010)31:10<2942::aid-immu2942

    authors: Villoslada P,Abel K,Heald N,Goertsches R,Hauser SL,Genain CP

    更新日期:2001-10-01 00:00:00

  • CD8alpha+ DC are not the sole subset cross-presenting cell-associated tumor antigens from a solid tumor.

    abstract::One of the clear paradoxes in tumor immunology is the fact that cross-presentation of cell-associated tumor antigens to CD8(+) T cells is efficient, yet CTL generation is weak, and tumors continue to grow. We examined, for the first time whether this may be due to alterations in the phenotype or function of cross-pres...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200940153

    authors: McDonnell AM,Prosser AC,van Bruggen I,Robinson BW,Currie AJ

    更新日期:2010-06-01 00:00:00