Abstract:
:Membrane-bound IgG was found only on old populations of platelets from normal individuals. This IgG could be dissociated from senescent cells by repeatedly heating the cells. Heat-eluted IgG (He-IgG) prepared from senescent red blood cells was capable of binding to either heat-treated old platelets or Vibrio cholerae neuraminidase (VCN)-treated young platelets, suggesting expression of a common age-dependent antigen on the senescent red blood cells and old platelets. We analyzed the role of membrane-bound IgG in the immune elimination of aging platelets by direct phagocytosis of different platelet subpopulations by autologous monocytes in vitro. While removal of He-IgG from old platelets inhibited their phagocytosis, preincubation of either heat-treated old or VCN-treated young platelets promoted phagocytosis of these cells by autologous monocytes. The phagocytosis of senescent cells required intact IgG on these cells. Either removal of Fc fragments from He-IgG or treatment of autologous monocytes with Fc fragments prior to the phagocytosis assay resulted in a marked reduction of phagocytosis (greater than 75%). We conclude that Fc receptors on the monocytes and the presence of membrane-specific IgG are crucial elements for immune elimination of senescent platelets.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Khansari N,Fudenberg HHdoi
10.1002/eji.1830131207subject
Has Abstractpub_date
1983-12-01 00:00:00pages
990-4issue
12eissn
0014-2980issn
1521-4141journal_volume
13pub_type
杂志文章,收录出版abstract::The involvement of the CD2 (T11) molecule, an alternative activation pathway for T lymphocytes, in the regulation of tumor necrosis factor (TNF)-alpha/TNF receptor system in human T lymphocytes has been investigated. It has been found that both TNF-alpha synthesis and secretion were induced after incubation of purifie...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221219
更新日期:1992-12-01 00:00:00
abstract::Macrophage inflammatory protein-3alpha (MIP-3alpha)/CCL20 and MIP-3beta/CCL19 are members of the CC chemokine subfamily which exert their effects through specific receptors, CCR6 and CCR7, respectively. Previously, we have reported that human neutrophils have the capacity to produce a number of chemokines, including I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<1981::aid-immu19
更新日期:2001-07-01 00:00:00
abstract::Mercuric chloride (HgCl2) has contrasting effects on different rat strains: susceptible strains, e.g. Brown Norway (BN) develop polyclonal B cell activation, multiple autoantibodies and widespread tissue injury. Lewis (LEW) rats are resistant: no autoimmune response occurs after HgCl2; instead, there is immunosuppress...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261018
更新日期:1996-10-01 00:00:00
abstract::We have developed a long-term culture system using the murine bone marrow stromal cells MS-5 to support the growth of progenitor B cells with CD34-, CD10+, CD19+, and cytoplasmic mu chain (C mu)-negative surface phenotype from human CD34+ cells purified from umbilical cord blood (CB). When 10(3) CD34+ cells/well were ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<855::AID-IMMU
更新日期:1998-03-01 00:00:00
abstract::This Viewpoint series provides authoritative and detailed outlines of exciting areas of DC research. Some of the subjects that frequently come up include development of DC; distribution of DC in lymphoid and non-lymphoid tissues such as skin, intestine and lung; different forms or subsets of DC; and the role of DC in ...
journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.201040733
更新日期:2010-08-01 00:00:00
abstract::All leukocytes express the cell surface glycoprotein CD45, which has intrinsic intracellular protein tyrosine phosphatase activity. CD45 is known to play a regulatory role in activation-induced signaling in lymphocytes; however, little is known of its role in non-lymphoid leukocytes. Therefore, we examined the potenti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199905)29:05<1467::AID-IMM
更新日期:1999-05-01 00:00:00
abstract::Recently, it has been shown that cloned L1/1 T helper cells of type 2 (TH2-cells), when stimulated with antigen, are able to induce polyclonal B cell proliferation. Here we present evidence that this process is dependent on direct cell-cell interaction between T and B cells, which in the effector phase, i.e., during s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190113
更新日期:1989-01-01 00:00:00
abstract::A human monoclonal IgE from patient DES, IgE (DES), has been shown to activate the classical pathway of complement. The mechanism of this activation has been investigated and can be summarized as follows: (a) IgE (DES) is able to bind and activate C1 in a dose-dependent fashion. This activation increases with the size...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140310
更新日期:1984-03-01 00:00:00
abstract::The magnitude of the output of lymphocytes from Peyer's patches (PP), the morphology of the lymphocytes and their route of exit from PP has been examined in sheep. An extracorporeal perfusion system was used to selectively label a 3-4 m length of the terminal ileum of lambs at 6-10 weeks of age (the mesenteric lymph n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140103
更新日期:1984-01-01 00:00:00
abstract::Human cytomegalovirus (HCMV) employs multiple mechanisms to evade the immune system and succeeds to persist lifelong in the host. Human dendritic cells (DC) are the main antigen-presenting cells and play the key role in inducing and maintaining immune responses. Here, we studied the interaction of HCMV with DC. We fou...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323612
更新日期:2003-06-01 00:00:00
abstract::Intravenous immunoglobulins (IVIgs) efficiently suppress a variety of autoimmune diseases. Over the past few years several potential mechanisms underlying this antiinflammatory activity have become apparent. Among these, terminal sialic acid residues in the sugar moiety of the immunoglobulin G constant fragment have b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242710
更新日期:2012-12-01 00:00:00
abstract::The functional relevance of CD2-mediated signal transduction in triggering T cell receptor/CD3-independent natural killer cell cytotoxicity was investigated by introducing wild-type CD2 or signaling-deficient CD2 with truncated cytoplasmic region into a murine natural killer-like large granular lymphocyte clone lackin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210345
更新日期:1991-03-01 00:00:00
abstract::In June 2009, the National Institute of Allergy and Infectious Diseases (NIAID), Division of Allergy, Immunology and Transplantation (DAIT), sponsored a workshop entitled Mast Cells in Innate and Adaptive Immunity. International experts in mast cell biology discussed recent advances in the field and future areas of re...
journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.200990325
更新日期:2010-01-01 00:00:00
abstract::Mice deficient for the adapter protein SLP65 (BLNK) show a partial block in early B cell development, reduced numbers of mature B cells in the periphery, an absence of B1 cells and a reduction of IgM and IgG3 serum immunoglobulin levels. A strikingly similar phenotype is observed in Btk-deficient mice. To investigate ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<2164::aid-immu2164>3
更新日期:2001-07-01 00:00:00
abstract::Here we demonstrate that components of the entire complement cascade are fixed on the surface of erythrocytes infected with the human malarial parasite Plasmodium falciparum. Despite the activation of lytic complement factors, no complement-mediated lysis of P. falciparum-infected erythrocytes occurred only in the abs...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271034
更新日期:1997-10-01 00:00:00
abstract::The encephalitogenic peptide pMOG 35-55 from the myelin oligodendrocyte glycoprotein was used to induce experimental autoimmune encephalomyelitis (EAE) in H-2b mice with the interleukin-6 (IL-6) gene intact or disrupted. The IL-6+/+ mice developed a chronic form of EAE ascending paralysis, whereas the IL-6-/- mice wer...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199805)28:05<1727::AID-IMM
更新日期:1998-05-01 00:00:00
abstract::The thymic medulla is composed of distinct epithelial cell subsets, defined in this report by the reactivity of two novel antibodies, 95 and 29, raised against mouse thymic epithelial cell lines. These antibodies were used to probe the development of medulla in wild-type or mutant thymuses. In CD3 epsilon-deficient mi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270615
更新日期:1997-06-01 00:00:00
abstract::Rapid IgE desensitization provides temporary tolerization for patients who have presented severe hypersensitivity reactions to food and drugs, protecting them from anaphylaxis, but the underlying mechanisms are still incompletely understood. Thus, here we develop an effective and reproducible in vitro model of rapid I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040810
更新日期:2011-04-01 00:00:00
abstract::A large panel of T cell clones (TCC) specific for the recombinant form of Poa pratensis allergen (rKBG7.2 or Poa p9) were established from the peripheral blood of grass pollen-sensitive donor in the absence or presence of recombinant interferon-alpha (IFN-alpha) in bulk culture and their pattern of cytokine secretion,...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260328
更新日期:1996-03-01 00:00:00
abstract::Fas is a cell surface molecule that is expressed on a wide array of cell types and triggers apoptosis. While in most situations Fas ligation activates programmed cell death, on resting T lymphocytes it can co-stimulate proliferation with the T cell receptor (TCR)/CD3 complex. This incongruity suggests that Fas may eli...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260505
更新日期:1996-05-01 00:00:00
abstract::Despite the presence of tumor-specific effector cells in the circulation of cancer patients, the immune response of the majority of these patients is not sufficient to prevent the growth and spread of their tumors. That tumor cells can be killed in vitro by tumor-reactive cytotoxic T cells is testimony to the fact tha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200424938
更新日期:2004-06-01 00:00:00
abstract::In contrast to previous studies, we report that heterologous anti-delta antibodies can act as a powerful multi-class humoral immunosuppressant. Primary direct plaque-forming cell (IgM) responses of BALB/c mice injected from birth with a rabbit anti-delta antiserum were reduced to 3% of control levels against a T-depen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160816
更新日期:1986-08-01 00:00:00
abstract::An effective immune response against the intracellular pathogen Mycobacterium tuberculosis is strictly dependent on T cell activation. Although this protective response mainly depends on local release of pro-inflammatory cytokines by Th1 CD4(+) T cells, contribution of Vgamma9 / Vdelta2 T lymphocytes to immune protect...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(200005)30:5<1512::AID-IMMU
更新日期:2000-05-01 00:00:00
abstract::IL-33 is a novel multi-functional IL-1 family member that, in contrast to other family members, is associated with Th2 responses. IL-33 signals via a heterodimer composed of its receptor, IL-1 receptor-like-1 (IL-1RL1), more commonly known as ST2L, and the IL-1R accessory protein. ST2L is expressed by endothelial cell...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200940026
更新日期:2009-12-01 00:00:00
abstract::The variable region nucleotide sequences of seven (five IgM and two IgG) anti-histone monoclonal antibodies from a single MRL/Mp-lpr/lpr mouse have been determined. These antibodies are not clonally related and used diverse V, D and J genes. However, six of the seven antibodies have VH segments encoded by genes from t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210721
更新日期:1991-07-01 00:00:00
abstract::Bone marrow (BM) stromal cells are important in the development and maintenance of cells of the immune system. Using single cell RNA sequencing, we here explore the functional and phenotypic heterogeneity of individual transcriptomes of 1167 murine BM mesenchymal stromal cells. These cells exhibit a tremendous heterog...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848053
更新日期:2019-09-01 00:00:00
abstract::IL-10 plays an important role in the regulation of immune responses. We and others have demonstrated recently that cyclic adenosine monophosphate (cAMP)-elevating substances up-regulate monocytic IL-10 expression in vitro and in vivo. Computer analysis of the IL-10 promoter/enhancer region localized four putative cAMP...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199910)29:10<3098::AID-IMM
更新日期:1999-10-01 00:00:00
abstract::Two monoclonal anti-idiotypic antibodies (HP-Id20 and HP-Id22) recognizing two different public idiotopes expressed in the anti-poly(Glu60,Ala30,Tyr10) (GAT) response were used to immunize BALB/c and C57BL/6 mice. From these animals hybridomas were isolated. From BALB/c and C57BL/6 mice eight and seven monoclonal anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150609
更新日期:1985-06-01 00:00:00
abstract::We have studied the kinetics of the antigen induced response of naive and memory CD8 T cells expressing a transgenic T cell receptor (TCR) specific for the glycoprotein peptide amino acid 33-41 (GP33) of the lymphocytic choriomeningitis virus (LCMV). Memory T cells were generated in vivo by adoptive transfer of LCMV T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199901)29:01<284::AID-IMMU
更新日期:1999-01-01 00:00:00
abstract::A simple and efficient procedure for the construction of bifunctional molecules is described and their use in a variety of applications documented. This procedure is based on our observation that mouse IgG1 monoclonal antibodies, when mixed with equimolar amounts of a high-affinity rat monoclonal antibody specific for...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160615
更新日期:1986-06-01 00:00:00