Abstract:
:All leukocytes express the cell surface glycoprotein CD45, which has intrinsic intracellular protein tyrosine phosphatase activity. CD45 is known to play a regulatory role in activation-induced signaling in lymphocytes; however, little is known of its role in non-lymphoid leukocytes. Therefore, we examined the potential effect of CD45 on chemokine-induced signaling in human neutrophils (polymorphonuclear cells, PMN). Treating isolated PMN for 2 h with an anti-CD45RB antibody (Bra11) down-modulated expression of the chemokine receptors CXCR1 and CXCR2 to 44 +/- 10% and 47 +/- 9% of their respective controls. The tyrosine kinase inhibitors genistein and herbimycin A significantly inhibited the Bra11-induced down-modulation of CXCR1 and CXCR2. Furthermore, Bra11-treated PMN were functionally inhibited in their capacity to exhibit IL-8-induced transient intracellular Ca2+ increases. Selected targeting of CXC receptors is indicated by the fact that N-formyl-Met-Leu-Phe (fMLP) receptor expression and function were not lost following Bra11 treatment. The effect of Bra11 on IL-8-mediated function and receptor expression was paralleled by decreased tyrosine phosphorylation of a 54- to 60-kDa protein. These findings indicate that CD45 can act to modulate PMN responses to chemokines; thus agents regulating CD45 can potentially modulate leukocyte traffic and may represent a novel therapeutic approach towards the treatment of inflammatory diseases.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Mitchell GB,Khandaker MH,Rahimpour R,Xu L,Lazarovits AI,Pickering JG,Suria H,Madrenas J,Pomerantz DK,Feldman RD,Kelvin DJdoi
10.1002/(SICI)1521-4141(199905)29:05<1467::AID-IMMkeywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
1467-76issue
5eissn
0014-2980issn
1521-4141journal_volume
29pub_type
杂志文章abstract::Many pathogens are initially encountered in the gut, where the decision is made to mount an immune response or induce tolerance. The mesentric lymph node (mLN) has been shown to be involved in immune response and much more in oral tolerance induction. Furthermore, using an in vivo transplantation model, we showed rece...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040681
更新日期:2011-03-01 00:00:00
abstract::Ikaros is a transcriptional regulator whose function is essential for B cell development. It is expressed in the hematopoietic stem cell (HSC) through the mature B cell stage. Using genetically engineered mice in which the endogenous Ikaros gene is disrupted, it has been shown that a lack of Ikaros leads to a block in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200637026
更新日期:2007-04-01 00:00:00
abstract::Effects of carrageenan (CAR) treatment on the response of interferon (IFN) production in vivo and in vitro after stimulation with an IFN-gamma inducer, staphylococcal enterotoxin A (SEA), was investigated. The IFN-gamma production in mice stimulated with SEA was impaired after i.v. administration of a 20 mg/kg dose of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160411
更新日期:1986-04-01 00:00:00
abstract::A mouse T cell hybrid specific for pigeon cytochrome c in the context of I-Ek responds by secreting interleukin 2 when co-cultured with the native antigen and the B cell lymphoma, LK-35.2, or naive splenic B cells as antigen-presenting cells (APC). Cytochromes c and their corresponding C-terminal fragments which are n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160702
更新日期:1986-07-01 00:00:00
abstract::Interleukin-12 (IL-12) and Flt3 ligand (FL) regulate hematopoiesis by stimulating proliferation, differentiation and migration of progenitor and/or stem cells. In this study, we evaluated the in vivo effect of IL-12 alone or in combination with FL on dendritic cell (DC) generation and accumulation in murine spleen, ly...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200009)30:9<2565::AID-IMMU2565>3
更新日期:2000-09-01 00:00:00
abstract::A majority of immunodominant epitopes of mycobacterial antigens are known to be recognized by murine T cells in the context of several H-2 haplotypes. In this study, we established the frequency of T cells able to recognize these peptides promiscuously, i.e. in the context of allogeneic antigen-presenting cells, using...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251009
更新日期:1995-10-01 00:00:00
abstract::The CD11c(+) cell population in the non-parenchymal cell population of the mouse liver contains dendritic cells (DC), NK cells, B cells and T cells. In the hepatic CD11c(+) DC population from immunocompetent or immunodeficient [recombinase-activating gene-1 (RAG1)(-/-)] C57BL/6 mice (rigorously depleted of T cells, B ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324336
更新日期:2004-02-01 00:00:00
abstract::The physical association of CD5 and the T cell antigen receptor (TcR)/CD3 complex on the surface of intact human lymphocytes was investigated using co-capping experiments and fluorescence resonance energy transfer (FRET) analyses. Antibody-induced capping of CD5 or CD3 and double indirect immunofluorescence labeling r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230530
更新日期:1993-05-01 00:00:00
abstract::Caspases are essential mediators of cytokine release and apoptosis. Additionally, caspase activity is required for the proliferation of naive T lymphocytes. It remained unclear how proliferating cells are able to cope with the pro-apoptotic activity especially of effector caspases-3 and -7. Possible reasons might incl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838211
更新日期:2008-07-01 00:00:00
abstract::Dopamine by itself has not up to now been reported to activate T cell function. We show here that dopamine interacts directly with dopaminergic receptors on normal human T cells and triggers beta1 integrin-mediated T cell adhesion to a major extracellular matrix component, fibronectin (FN). Such adhesion is a characte...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200112)31:12<3504::aid-immu3504>
更新日期:2001-12-01 00:00:00
abstract::B cell-activating factor belonging to the TNF family (BAFF) and its receptor BAFF-R play critical roles in the maturation and survival of conventional peripheral B cells. However, they appeared to be dispensable for the generation and maintenance of CD5(+) B-1 cells as BAFF(-/-) and BAFF-R(-/-) mice have normal B-1 ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200635956
更新日期:2006-07-01 00:00:00
abstract::Maintenance of homeostasis at body barriers that are constantly challenged by microbes, toxins and potentially bioactive (macro)molecules requires complex, highly orchestrated mechanisms of protection. Recent discoveries in respiratory research have shed light on the unprecedented role of airway epithelial cells (AEC)...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201847811
更新日期:2020-05-01 00:00:00
abstract::The effector function of activated CD4(+) T cells and secretion of cytokines are important in the establishment of productive immune responses and tolerance. We identified expression by CD4(+) T cells of Notch receptors and ligands and enhanced Notch signaling upon activation. Notch1 expression was up regulated and co...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425562
更新日期:2005-03-01 00:00:00
abstract::Nonhematopoietic stromal cells contribute to the organization and homeostasis of secondary lymphoid organs by producing cytokines and chemokines. The development and maintenance of these stromal cells are thought to be regulated by innate immune cells. Indeed, we recently showed that signal regulatory protein α (SIRPα...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948103
更新日期:2019-09-01 00:00:00
abstract::CD11a/CD18 (beta2)-integrins are expressed on leukocytes and are involved in cell adhesion and signaling. Despite extensive studies the signaling pathways and molecular mechanisms involved in integrin regulation in T cells remain not completely understood. We have now studied the involvement of the tyrosine kinase Lck...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1670::AID-IMMU1670>3
更新日期:2002-06-01 00:00:00
abstract::Mice deficient for the adapter protein SLP65 (BLNK) show a partial block in early B cell development, reduced numbers of mature B cells in the periphery, an absence of B1 cells and a reduction of IgM and IgG3 serum immunoglobulin levels. A strikingly similar phenotype is observed in Btk-deficient mice. To investigate ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<2164::aid-immu2164>3
更新日期:2001-07-01 00:00:00
abstract::The CBA/N mouse carries the X-linked immunodeficiency xid, resulting in defective B cell development. B cells from these animals cannot mount antibody responses to type 2 T-independent antigens, and do not synthesize DNA when stimulated with anti-immunoglobulin (Ig) antibodies which are mitogenic for normal B cells. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240123
更新日期:1994-01-01 00:00:00
abstract::Anti-HLA donor-specific antibodies are deleterious for organ transplant survival. Class I HLA donor-specific antibodies are identified by using the Luminex single antigen beads (LSAB) assay, which also detects anti-denatured HLA antibodies (anti-dHLAs). Anti-dHLAs are thought to be unable to recognize native HLA (nHLA...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445340
更新日期:2015-07-01 00:00:00
abstract::The immune response against human adenovirus (HAdV) has gained interest because of the application of HAdV-based vectors in gene therapy and the high incidence of infections in pediatric recipients of allogeneic stem cell grafts. Because antiviral medication is frequently ineffective, the option of adoptive transfer o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535786
更新日期:2006-09-01 00:00:00
abstract::Transgenic mice in which mouse interleukin (IL)-7 cDNA is expressed under the control of the mouse major histocompatibility complex (MHC) class II (E alpha) promoter develop a lymphoproliferative disease characterized by the early polyclonal expansion of T cells followed in many cases by the development of lymphomas o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260105
更新日期:1996-01-01 00:00:00
abstract::Mast cells are important cellular constituents of epithelial-mesenchymal interactions, densely located at sites of microbial entry into the host where they are continuously exposed to products from commensals. In order to avoid excessive activation and the associated pathology, mast cell responses to TLR agonists must...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242786
更新日期:2013-04-01 00:00:00
abstract::The influence of muramyl dipeptide (N-acetylmuramyl-L-alanyl-D-isoglutamine) on the rejection of mouse skin allografts was investigated. While the 0.1-mg dose administered on days 7, 6, 5 prior to transplantation caused significant prolongation of the graft survival, the 0.5-mg dose administered on days 3, 2, 1 prior ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131015
更新日期:1983-10-01 00:00:00
abstract::When cytotoxic titrations were made of rabbit anti-mouse brain and anti-Thy-1.2 sera against mouse lymphoid cells in the presence of sodium barbital or xylocaine, it was observed that a proportion of T cells were much more sensitive (50 x) than the remainder. This effect could be detected with ethidium or fluorescein ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050408
更新日期:1975-04-01 00:00:00
abstract::The contribution of Gal alpha 1,3Gal (alpha-Gal) to cell-mediated organ xenograft rejection is controversial. We have used recombinant lentiviruses encoding a porcine alpha 1,3 galactosyltransferase (alpha 1,3GalT) to obtain alpha-Gal-expressing primary human aortic endothelial cells (HAEC) at a frequency of 70-90%. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324683
更新日期:2004-04-01 00:00:00
abstract::Here we show that tumor cells (TC) from renal cancers regulate the migratory properties of autologous tumor-infiltrating lymphocytes (TIL), enhancing their ability to invade the extracellular matrix. A similar effect is exerted by human recombinant macrophage chemotactic protein 1 (MCP-1) and IL-8, chemokines known to...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199808)28:08<2530::AID-IMM
更新日期:1998-08-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis, an experimental murine model for multiple sclerosis, is induced by stimulation of myelin-specific T lymphocytes. Myelin oligodendrocyte glycoprotein (MOG), a minor component of myelin proteins, is a potent autoantigen which contributes extensively to the anti-myelin response....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535021
更新日期:2006-03-01 00:00:00
abstract::Regulatory T cells (Treg) are vital in maintaining the homeostasis of immune reactions. In chronic infections, such as schistosomiasis, it remains unclear whether engagement of the TLR family is required to induce Treg activity. Thus, we performed in vivo studies using TLR2-/- mice infected with Schistosoma mansoni an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737063
更新日期:2007-08-01 00:00:00
abstract::Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays an important role in regulating the local inflammatory immune response, but regulatory mechanisms of this cytokine have not been fully elucidated. Here, we demonstrate that IL-10 deficiency renders LPS treatment ineffective in regulating the expression...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141733
更新日期:2012-06-01 00:00:00
abstract::An anti-T cell receptor (TcR) monoclonal antibody (mAb), LC4, directed against a human leukemic T cell line, SUP-T13, caused DNA fragmentation ("apoptosis") and cell death upon binding to this cell line. Cross-linking of receptor molecules was necessary for this effect since F(ab')2, but not Fab', fragments of LC4 cou...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191023
更新日期:1989-10-01 00:00:00
abstract::Following immunization with a complex antigen, a B cell internalizes this antigen through an interaction between its surface immunoglobulins and an epitope of the antigen. Enzymatic processing of the antigen frees one or more short peptide determinants (TD) which bind to class II molecules of the B cell. If the comple...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210633
更新日期:1991-06-01 00:00:00