Abstract:
:Here we show that tumor cells (TC) from renal cancers regulate the migratory properties of autologous tumor-infiltrating lymphocytes (TIL), enhancing their ability to invade the extracellular matrix. A similar effect is exerted by human recombinant macrophage chemotactic protein 1 (MCP-1) and IL-8, chemokines known to increase T lymphocyte migration both across vascular endothelium and subendothelial matrix. We found that TC freshly derived from renal cell carcinoma surgical specimens constitutively secrete both IL-8 and MCP-1 and that TIL express both specific receptors. TIL matrix invasion elicited by TC is inhibited by the addition of neutralizing antisera specific for IL-8 and MCP-1, demonstrating the direct relationship between chemokine release by TC and TIL invasion. Of note, TIL invasion of the extracellular matrix requires the alpha1 integrin, which acts through its I-domain that is upregulated upon culture with MCP-1 and IL-8. Collectively, these findings suggest that TC may actively recruit TIL via the release of chemotactic factors that enhance an alpha1 integrin-mediated pathway of matrix invasion.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Ferrero E,Fabbri M,Poggi A,Galati G,Bernasconi S,Zocchi MRdoi
10.1002/(SICI)1521-4141(199808)28:08<2530::AID-IMMsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
2530-6issue
8eissn
0014-2980issn
1521-4141pii
10.1002/(SICI)1521-4141(199808)28:08<2530::AID-IMMjournal_volume
28pub_type
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