当前位置： SCI文献检索 > EUROPEAN JOURNAL OF IMMUNOLOGY期刊下所有文献 > Co-expression of TNFR2 and CD25 identifies more of the functional CD4+FOXP3+ regulatory T cells in human peripheral blood.

Co-expression of TNFR2 and CD25 identifies more of the functional CD4+FOXP3+ regulatory T cells in human peripheral blood.

Abstract：

:Previously, we found that co-expression of CD25 and TNFR2 identified the most suppressive subset of mouse Treg. In this study, we report that human peripheral blood (PB) FOXP3(+) cells present in CD25(high), CD25(low) and even CD25(-) subsets of CD4(+) cells expressed high levels of TNFR2. Consequently, TNFR2-expressing CD4(+)CD25(+) Treg included all of the FOXP3(+) cells present in the CD4(+)CD25(high) subset as well as a substantial proportion of the FOXP3(+) cells present in the CD4(+)CD25(low) subset. Flow cytometric analysis of PB identified five-fold more Treg, determined by FOXP3 expression, in the CD4(+)CD25(+)TNFR2(+) subset than in the CD4(+)CD25(high) subset. In addition, similar levels of FOXP3(+) cells were identified in both the CD4(+)CD25(+)TNFR2(+) and CD4(+)CD25(+)CD127(low/-) subsets. Furthermore, the CD4(+)CD25(+)TNFR2(+) subset expressed high levels of CTLA-4, CD45RO, CCR4 and low levels of CD45RA and CD127, a phenotype characteristic of Treg. Upon TCR stimulation, human PB CD4(+)CD25(+)TNFR2(+) cells were anergic and markedly inhibited the proliferation and cytokine production of co-cultured T-responder cells. In contrast, CD4(+)CD25(+)TNFR2(-) and CD4(+)CD25(-)TNFR2(+) T cells did not show inhibitory activity. As some non-Treg express TNFR2, the combination of CD25 and TNFR2 must be used to identify a larger population of human Treg, a population that may prove to be of diagnostic and therapeutic benefit in cancer and autoimmune diseases.

journal_name

Eur J Immunol

journal_title

European journal of immunology

authors

Chen X,Subleski JJ,Hamano R,Howard OM,Wiltrout RH,Oppenheim JJ

doi

10.1002/eji.200940022

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

1099-106

issue

4

eissn

0014-2980

issn

1521-4141

journal_volume

40

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF IMMUNOLOGY文献大全
  • Maternal immunization modulates the primary immune response to 2-phenyl-oxazolone in BALB/c mice.

    abstract：:The development of the antibody repertoire in newborn mice is greatly influenced by idiotype network interactions. It has been demonstrated that anti-idiotypic antibodies either directly injected or transferred from the mother may alter the repertoire for life. For an elucidation of the underlying mechanisms we have a...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830241216

    authors： Lemke H,Lange H,Berek C

    更新日期：1994-12-01 00:00:00

  • Role of the thymus in the generation of skin-homing alpha beta and gamma delta virgin T cells.

    abstract：:Current models of lymphocyte traffic suggest that homing specificities of T cells to tissues such as skin are generated outside the thymus as a result of activation of naive T cells by antigen in lymph nodes. Virgin T cells are thought to home to high endothelial venules in lymph nodes, but are thought to be unable to...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830250314

    authors： Washington EA,Kimpton WG,Holder JE,Cahill RN

    更新日期：1995-03-01 00:00:00

  • Proteins in normal and malignant cells, cross-reacting with the latent membrane protein encoded by Epstein-Barr virus.

    abstract：:Human B lymphocytes transformed by infection with the Epstein-Barr virus (EBV) express a new membrane protein of 63 kDa (latent membrane protein, LMP) encoded by the virus. The function of this protein in the virus-cell interaction is not known. In this work we have identified in EBV- human and mouse cell molecules wh...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830180820

    authors： Hatzubai A,Lerner RA,Klein G,Sulitzeanu D

    更新日期：1988-08-01 00:00:00

  • Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation.

    abstract：:Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. Since Stx11 mutations are causally associated with a familial hemophagocytic lymphohistio-cytosis, we wanted to clarify whether STX11 is functionally important for key immune cell populations. This was studied in primary cells obtain...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.201142343

    authors： D'Orlando O,Zhao F,Kasper B,Orinska Z,Müller J,Hermans-Borgmeyer I,Griffiths GM,Zur Stadt U,Bulfone-Paus S

    更新日期：2013-01-01 00:00:00

  • Aggravation of experimental cutaneous leishmaniasis in mice by administration of interleukin 3.

    abstract：:Previous studies from our laboratory have shown that some in vitro maintained Leishmania major-specific L3T4+ T cells were capable of exacerbating cutaneous leishmaniasis after adoptive transfer to normal syngeneic mice. Results presented in this report show that these cells released substantial amounts of interleukin...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830180815

    authors： Feng ZY,Louis J,Kindler V,Pedrazzini T,Eliason JF,Behin R,Vassalli P

    更新日期：1988-08-01 00:00:00

  • Modulation of B and T cell subsets in mice treated with fractionated total lymphoid irradiation. I. Blockage of differentiating B cell pathways.

    abstract：:The effect of fractionated total lymphoid irradiation (TLI) on the maturation of B cells was examined in mice. At various times after irradiation of the mice, their spleen cells were tested for the mitogenic responses to dextran sulfate and lipopolysaccharide. In parallel the cells were stained with fluorescent anti-T...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830130109

    authors： Zan-Bar I

    更新日期：1983-01-01 00:00:00

  • Delay in expression of a mammary tumor provirus is responsible for defective clonal deletion during postnatal period.

    abstract：:A gene-encoding ligand for deletion of T cells bearing TcRV beta 6 and V beta 8.1 cosegregates a new mammary tumor provirus locus, Mtv-50 in NC mice. The sequence of the open reading frame (ORF) in the 3' long terminal repeat (LTR) of Mtv-50 was strikingly similar to those of Mtv-7, Mtv-43 and exogenous mouse mammary ...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830240235

    authors： Niimi N,Wajjwalku W,Ando Y,Tomida S,Takeuchi M,Ueda M,Kaneda T,Yoshikai Y

    更新日期：1994-02-01 00:00:00

  • Expression of interleukin (IL)-1 beta, IL-6 and their respective receptors in the normal rat brain and after injury.

    abstract：:The expression of interleukin (IL)-1 beta, IL-6 and their respective receptors has been studied in the rat brain before and up to 24 h after injury. Messenger RNA transcripts of these four genes were detected by in situ hybridization (ISH) in different structures of the intact brain. The distribution was very similar ...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830221131

    authors： Yan HQ,Banos MA,Herregodts P,Hooghe R,Hooghe-Peters EL

    更新日期：1992-11-01 00:00:00

  • Binding of lipopeptide to CD14 induces physical proximity of CD14, TLR2 and TLR1.

    abstract：:Lipoproteins or lipopeptides (LP) are bacterial cell wall components detected by the innate immune system. For LP, it has been shown that TLR2 is the essential receptor in cellular activation. However, molecular mechanisms of LP recognition are not yet clear. We used a FLAG-labeled derivative of the synthetic lipopept...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.200425336

    authors： Manukyan M,Triantafilou K,Triantafilou M,Mackie A,Nilsen N,Espevik T,Wiesmüller KH,Ulmer AJ,Heine H

    更新日期：2005-03-01 00:00:00

  • Phenotypical and functional characterization of Fc gamma receptor I (CD64)-negative monocytes, a minor human monocyte subpopulation with high accessory and antiviral activity.

    abstract：:Fc gamma receptor I-positive (CD64+) and Fc gamma receptor I-negative (CD64-) monocytes were prepared from highly purified (elutriation-derived) human monocytes by cytofluorograph cell sorting, and a phenotypical and functional dissociation of the isolated CD64+ and CD64- monocyte subsets is demonstrated. Surface anal...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830231213

    authors： Grage-Griebenow E,Lorenzen D,Fetting R,Flad HD,Ernst M

    更新日期：1993-12-01 00:00:00

  • Active suppression of the class II transactivator-encoding AIR-1 locus is responsible for the lack of major histocompatibility complex class II gene expression observed during differentiation from B cells to plasma cells.

    abstract：:In this study the genetic control of major histocompatibility complex (MHC) class II gene expression during the transition from B cell to plasma cell has been analyzed. Class II molecules are not expressed in plasma cells because of an active suppression resulting in the abrogation of class II gene transcription. We s...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830261028

    authors： Sartoris S,Tosi G,De Lerma Barbaro A,Cestari T,Accolla RS

    更新日期：1996-10-01 00:00:00

  • Selection of dual Valpha T cells.

    abstract：:Incomplete allelic exclusion of TCRa gene rearrangement permits the generation of dual Valpha T cells, though the issues of their frequency and whether both alphabeta pairs participate in thymic selection have not been resolved. Both questions have been investigated using lymphocytes from mice hemizygous at the TCRa l...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/(SICI)1521-4141(199807)28:07<2115::AID-IMM

    authors： Elliott JI

    更新日期：1998-07-01 00:00:00

  • Epitope specificity of the T cell proliferative response to lysozyme: proliferative T cells react predominantly to different determinants from those recognized by B cells.

    abstract：:The fine specificity of murine B 10.A/SgSn (B 10.A) T cells reactive with hen egg-white lysozyme (HEL) has been studied through the use of reduced, carboxymethylated HEL, a set of peptides encompassing the entire molecule, and a set of variant lysozymes from other species. Cells were taken from the lymph nodes drainin...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830100705

    authors： Maizels RM,Clarke JA,Harvey MA,Miller A,Sercarz EE

    更新日期：1980-07-01 00:00:00

  • Response of human B cells to different anti-immunoglobulin isotypes: absence of a correlation between early activation events and cell proliferation.

    abstract：:Cross-linking of surface immunoglobulin (sIg) by antibodies against IgM, IgG and IgD activates B cells and in some circumstances can induce cell proliferation. We studied the potential link between anti-Ig-induced changes in the cytosolic free Ca2+ concentration ([Ca2+]i), inositol phosphate production and the ability...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830171209

    authors： Roifman CM,Mills GB,Stewart D,Cheung RK,Grinstein S,Gelfand EW

    更新日期：1987-12-01 00:00:00

  • Grancalcin (GCA) modulates Toll-like receptor 9 (TLR9) mediated signaling through its direct interaction with TLR9.

    abstract：:Toll-like receptors (TLRs) are playing important roles in stimulating the innate immune response and intensifying adaptive immune response against invading pathogens. Appropriate regulation of TLR activation is important to maintain a balance between preventing tumor activation and inhibiting autoimmunity. Toll-like r...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.201545567

    authors： Kim TW,Hong S,Talukder AH,Pascual V,Liu YJ

    更新日期：2016-03-01 00:00:00

  • A CD40/CD40L feedback loop drives the breakdown of CD8(+) T-cell tolerance following depletion of suppressive CD4(+) T cells.

    abstract：:Dendritic cells (DCs) are the key APCs not only for the priming of naïve T cells, but also for the induction and maintenance of peripheral T-cell tolerance. We have recently shown that cognate interactions between Foxp3(+) Tregs and steady-state DCs are crucial to maintain the tolerogenic potential of DCs. Using DIETE...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.201343738

    authors： Muth S,Schütze K,Hain T,Yagita H,Schild H,Probst HC

    更新日期：2014-04-01 00:00:00

  • Evidence for a stromal cell-dependent, self-renewing B cell population in lymphoid follicles of the ileal Peyer's patch of sheep.

    abstract：:Lymphoid follicles of the ileal Peyer's patch (PP) of young sheep function as the major source of B cells and a site of immunoglobulin (Ig) receptor diversification. However, extensive cell death in culture has restricted investigations of ileal PP follicular (iPf)B cell biology. We investigated the possibility that s...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830240220

    authors： Griebel PJ,Ferrari G

    更新日期：1994-02-01 00:00:00

  • T cells are necessary for ILC2 activation in house dust mite-induced allergic airway inflammation in mice.

    abstract：:Allergic asthma is a chronic inflammation of the airways mediated by an adaptive type 2 immune response. Upon allergen exposure, group 2 innate lymphoid cells (ILC2s) can be rapidly activated and represent an early innate source of IL-5 and IL-13. Here, we used a house dust mite (HDM)-driven asthma mouse model to stud...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.201546119

    authors： Li BW,de Bruijn MJ,Tindemans I,Lukkes M,KleinJan A,Hoogsteden HC,Hendriks RW

    更新日期：2016-06-01 00:00:00

  • Homeostatic proliferation and survival of naïve and memory T cells.

    abstract：:The immune system relies on homeostatic mechanisms in order to adapt to the changing requirements encountered during steady-state existence and activation by antigen. For T cells, this involves maintenance of a diverse repertoire of naïve cells, rapid elimination of effector cells after pathogen clearance, and long-te...

    journal_title：European journal of immunology

    pub_type： 杂志文章,评审

    doi：10.1002/eji.200939444

    authors： Boyman O,Létourneau S,Krieg C,Sprent J

    更新日期：2009-08-01 00:00:00

  • Functional characteristics of murine macrophages responding to migration inhibitory factors.

    abstract：:Mouse bone marrow cells differentiate in culture in the presence of L cell-conditioned medium to macrophages (M phi). Proliferation, release of plasminogen activator, expression of transglutaminase, random motility and response to a standard preparation of purified M phi migration inhibitory factor (MIF) was recorded ...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830141008

    authors： Michels E,Stenzinger W,Sorg C

    更新日期：1984-10-01 00:00:00

  • Normal immunosuppressive protein inhibition of human and murine lymphoblastoid cell line proliferation.

    abstract：:Normal immunosuppressive protein, prepared from human plasma by DEAE-cellulose chromatography, inhibits DNA synthesis in human cell lines of lymphocytes of both T and B origin. It also inhibits [3H]thymidine incorporation in mouse cell lines. Normal immunosuppressive protein was able to inhibit the proliferation of th...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830090216

    authors： Nelken D,Ovadia H,Hanna N

    更新日期：1979-02-01 00:00:00

  • Recombinant C5a enhances interleukin 1 and tumor necrosis factor release by lipopolysaccharide-stimulated monocytes and macrophages.

    abstract：:Lipopolysaccharide (LPS) is a potent inducer of interleukin 1 (IL 1) synthesis and release, and of tumor necrosis factor (TNF) secretion. Many signals can enhance the LPS-induced production of these cytokines. We have previously observed that addition of low amounts of normal human serum to the culture medium enhances...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830200204

    authors： Cavaillon JM,Fitting C,Haeffner-Cavaillon N

    更新日期：1990-02-01 00:00:00

  • B lineage-restricted rearrangement of a human Ig kappa transgene.

    abstract：:To study the control of immunoglobulin kappa light chain gene rearrangement, we generated transgenic mice carrying a germ-line human kappa minilocus (HK) containing the J kappa-proximal V gene, V kappa IV, the V-J intergenic region, the five J kappa segments and the C kappa gene. This construct includes the intronic, ...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：

    authors： Cavelier P,Nato F,Coquilleau I,Rolink A,Rougeon F,Goodhardt M

    更新日期：1997-07-01 00:00:00

  • MLR blast cells generated in mutant-standard strain combinations bind H-2K and H-2D antigens.

    abstract：:Strains CBA (M523) (= M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B 10.D2 (M504 (= M504) and b 10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CB...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830090208

    authors： Geib RW,Klein J

    更新日期：1979-02-01 00:00:00

  • Evidence that cathelicidin peptide LL-37 may act as a functional ligand for CXCR2 on human neutrophils.

    abstract：:LL-37, derived from human cathelicidin, stimulates immune responses in neutrophils. Although FPR2 and P2X7 were proposed as LL-37 receptors, we have shown that among 21 neutrophil receptors only CXCR2 was down-regulated by LL-37. LL-37 functions similarly to CXCR2-specific chemokines CXCL1 and CXCL7 in terms of recept...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.200939496

    authors： Zhang Z,Cherryholmes G,Chang F,Rose DM,Schraufstatter I,Shively JE

    更新日期：2009-11-01 00:00:00

  • HLA-A*0201 presents TAP-dependent peptide epitopes to cytotoxic T lymphocytes in the absence of tapasin.

    abstract：:Tapasin is a 48-kDa endoplasmic reticulum (ER)-resident glycoprotein that binds to the transporter associated with antigen processing (TAP) and mediates an interaction between TAP and newly synthesized MHC class I molecules. It is also essential for the proper antigen presenting function of HLA-A*0101 (HLA-A1), HLA-A*...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/(SICI)1521-4141(199810)28:10<3214::AID-IMM

    authors： Lewis JW,Sewell A,Price D,Elliott T

    更新日期：1998-10-01 00:00:00

  • Antibody-dependent cellular cytotoxicity against tumor cells. II. The promonocyte identified as effector cell.

    abstract：:Macrophage precursor cells were cultured from the bone marrow of mice in a liquid culture system in the presence of conditioned medium. To separate their different maturation stages, they were passed through a discontinuous Ficoll density gradient, treated with iron plus magnet or passed through glass bead columns. Th...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830090404

    authors： Domzig W,Lohmann-Matthes ML

    更新日期：1979-04-01 00:00:00

  • DNA polymerase kappa deficiency does not affect somatic hypermutation in mice.

    abstract：:Somatic hypermutation (SH) in B cells undergoing T cell-dependent immune responses generates high-affinity antibodies that provide protective immunity. Most current models of SH postulate the introduction of a nick into the DNA and subsequent replication-independent, error-prone short-patch synthesis by one or more DN...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/1521-4141(200211)32:11<3152::AID-IMMU3152>

    authors： Schenten D,Gerlach VL,Guo C,Velasco-Miguel S,Hladik CL,White CL,Friedberg EC,Rajewsky K,Esposito G

    更新日期：2002-11-01 00:00:00

  • Neuronal interferon-gamma immunoreactive molecule: bioactivities and purification.

    abstract：:An interferon (IFN)-gamma immunoreactive molecule, localized to small neurons in peripheral sensory ganglia (N-IFN-gamma), has been detected with two mouse monoclonal antibodies (DB1 and DB16) directed against different epitopes of rat IFN-gamma. To define N-IFN-gamma with regard to its protein characteristics and bio...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830240205

    authors： Olsson T,Kelic S,Edlund C,Bakhiet M,Höjeberg B,van der Meide PH,Ljungdahl A,Kristensson K

    更新日期：1994-02-01 00:00:00

  • Identification of a new epitope of the 4F2/44D7 molecular complex present on sarcolemma and isolated cardiac fibers.

    abstract：:The murine monoclonal antibody (mAb) CB43, raised against the K-562 erythroleukemia line, reacts with monocytes, tissue macrophages, thymocytes and with all the human lines tested but not with resting lymphocytes, large granular lymphocytes, granulocytes and erythrocytes. However, activated lymphocytes and natural kil...

    journal_title：European journal of immunology

    pub_type： 杂志文章

    doi：10.1002/eji.1830190102

    authors： Bellone G,Alloatti G,Levi R,Geuna M,Tetta C,Peruzzi L,Letarte M,Malavasi F

    更新日期：1989-01-01 00:00:00