Abstract:
:The effect of fractionated total lymphoid irradiation (TLI) on the maturation of B cells was examined in mice. At various times after irradiation of the mice, their spleen cells were tested for the mitogenic responses to dextran sulfate and lipopolysaccharide. In parallel the cells were stained with fluorescent anti-Thy-1 and various anti-Ig antisera, and were analyzed on a fluorescence-activated cell sorter (FACS). By comparison with normal spleen cells the cells of TLI-treated mice gave a high response to dextran sulfate and a low response to lipopolysaccharide. FACS analysis revealed that TLI-treated spleen contains elevated numbers of B cells bearing high IgM and low IgD on the surface. The B cells of TLI-treated mice retained these immature characteristics even two to four months after the last irradiation. These findings indicate that TLI causes a longlasting blockage in B cell maturation processes.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Zan-Bar Idoi
10.1002/eji.1830130109subject
Has Abstractpub_date
1983-01-01 00:00:00pages
35-40issue
1eissn
0014-2980issn
1521-4141journal_volume
13pub_type
杂志文章abstract::The cytokine interleukin 1 (IL 1) plays an important role in the induction of IL 2 secretion and high-affinity IL 2 receptor (IL 2R) expression by T cells. The events that follow binding of IL 1 to IL 1R, however, are still unknown. In this study we describe two variants of the murine thymoma EL4 (5D3 and D6/76) that ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190217
更新日期:1989-02-01 00:00:00
abstract::Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a member of the immunoglobulin gene superfamily (IgSF) which is expressed by activated leukocytes and thymic epithelial cells and is a ligand for the lymphocyte antigen CD6. Herein, we report on the isolation and characterization of cDNA clones encoding mous...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270625
更新日期:1997-06-01 00:00:00
abstract::NLRP3 inflammasome is a protein complex crucial to caspase-1 activation and IL-1β and IL-18 maturation. This receptor participates in innate immune responses to different pathogens, including the bacteria of genus Brucella. Our group recently demonstrated that Brucella abortus-induced IL-1β secretion involves NLRP3 in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848016
更新日期:2019-07-01 00:00:00
abstract::Cell ablation is a valuable complement to mutagenesis for experimentally defining specific cell functions in physiology and pathophysiology in small animal models. One of the most popular ablation strategies involves transgenic expression of a primate diphtheria toxin receptor (DTR) on murine cells that are otherwise ...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201847527
更新日期:2018-07-01 00:00:00
abstract::RNA from 170 different Ig-secreting hybridomas was hybridized with VH gene probes 7183, QUPC52, S107, J558, J606, 36-60, V31, X-24 and V-GAM 3.8, with the aim of comparing xid to non-xid mice, neonatal NZB to adult NZB, and the peritoneal cavity with the splenic compartments for VH gene family expression. Our results ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181217
更新日期:1988-12-01 00:00:00
abstract::Th cells can adopt a number of different phenotypes. We performed microarray-assisted mRNA profiling on antigen-stimulated, TCR transgenic murine splenocytes that were cultured in the presence of cytokines. Transcriptome snapshots of Th cells differentiating into Th1 and Th2 phenotypes were obtained at various time po...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242979
更新日期:2013-04-01 00:00:00
abstract::Dendritic cells isolated from sheep afferent lymph were examined for their ability to bind soluble protein and peptide antigens labeled with fluorescein both in in vitro assays and following intradermal injection of antigen in vivo. Analysis of dendritic cells by flow cytometry revealed weak direct binding of proteins...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201102
更新日期:1990-11-01 00:00:00
abstract::Mouse peritoneal macrophages were charged with IgG molecules in monomeric (mIgG), heat-aggregated (agIgG) or antigen-complexed (acIgG) form. Upon exposure to 37 degrees C, all bound IgG ligand types are redistributed on the cell surface due to the mobilization of their corresponding Fc receptor (FcR). The major findin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830091213
更新日期:1979-12-01 00:00:00
abstract::Peptides presented by MHC class I molecules are mostly derived from proteins synthesized by the antigen-presenting cell itself, while peptides presented by MHC class II molecules are predominantly from materials acquired by endocytosis. External antigens can also be presented by MHC class I molecules in a process refe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445156
更新日期:2015-02-01 00:00:00
abstract::The aim of this study was to investigate the effects of IL-17-producing T cells, including Th17 and Tc17 cells, on acute graft-versus-host disease (aGVHD) in patients who had undergone granulocyte colony-stimulating factor (G-CSF)-mobilised peripheral blood progenitor cell (PBPC) and G-CSF-primed bone marrow (G-BM) tr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040793
更新日期:2011-02-01 00:00:00
abstract::Crosslinking of Fc γ receptor II B (FcγRIIB) and the BCR by immune complexes (IC) can downregulate antigen-specific B-cell responses. Accordingly, FcγRIIB deficiencies have been associated with B-cell hyperactivity in patients with systemic lupus erythematosus and mouse models of lupus. However, we have previously sho...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940184
更新日期:2010-10-01 00:00:00
abstract::We examined the ability of oral or parenteral immunization with immune stimulating complexes containing ovalbumin (ISCOMS-OVA) to prime T cell proliferative and cytokine responses. A single subcutaneous immunization with ISCOMS-OVA primed potent antigen-specific proliferative responses in the draining popliteal lymph ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251019
更新日期:1995-10-01 00:00:00
abstract::Previous studies demonstrated that mannan is a potent inhibitor of splenic entry of lymphocytes and mediates its inhibitory effect at an unidentified site in the spleen rather than acting directly on lymphocytes. This report describes the in vivo site of action of mannan. In vivo localization studies with fluoresceina...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220804
更新日期:1992-08-01 00:00:00
abstract::The immaturity of the neonatal immune system in mice is associated with defective IFN-gamma production and Th2-biased immune responses. In this study, infants vaccinated at birth with BCG produced similar concentrations of IFN-gamma in response to PPD and showed similar frequencies of IFN-gamma-producing lymphocytes a...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/1521-4141(200105)31:5<1531::AID-IMMU1531>3
更新日期:2001-05-01 00:00:00
abstract::H-2 gene-dependent nonresponsiveness and responsiveness to H-2-matched male cells can be observed in cytotoxic assays in several mouse strains. H-2 genes of several low responder strains can complement each other in cis or trans position to produce high responders. One of the H-2 gene products functions at the level o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830070708
更新日期:1977-07-01 00:00:00
abstract::The development and the differentiation of CD8(+) T cells are dependent on IL-15. Here, we have studied the source and mechanism of how IL-15 modulates CD8(+) T cell-mediated Th1 immune responses by employing two delayed-type hypersensitivity (DTH) models. IL-15-deficient (IL-15(-/-)) mice or mice treated with soluble...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324545
更新日期:2003-12-01 00:00:00
abstract::Antigen-specific immunotherapy is an attractive strategy for cancer control. In the context of antiviral vaccines, adenoviral vectors have emerged as a favorable means for immunization. Therefore, we chose a strategy combining use of these vectors with another successful approach, namely linkage of the vaccine antigen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939543
更新日期:2009-10-01 00:00:00
abstract::Dendritic cells (DC) are known to support the activation of natural killer (NK) cells. However, little is known about the role for DC in NK-cell homeostasis. In order to investigate this question, a novel bacterial artificial chromosome transgenic mouse model was generated in which the diphtheria toxin receptor is exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838659
更新日期:2008-10-01 00:00:00
abstract::The covalent binding reaction of the third component of complement (C3) with rabbit IgG immune aggregates has been studied by enzymic digestion of C3b-IgG adducts. In these adducts C3b was radioactively labeled in the free thiol group generated during activation of the internal thioester of C3. Trypsin digestion of 14...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240316
更新日期:1994-03-01 00:00:00
abstract::This article describes the study of the functional relationship between auto-tumor-reactive CD4(+) T cell clones (TCC) and autologous malignant B cells. Four auto-tumor-reactive CD4(+) TCC were derived from tumor-infiltrating T lymphocytes (TIL-T) from a freshly isolated human follicular lymphoma by the following tech...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199910)29:10<3188::AID-IMM
更新日期:1999-10-01 00:00:00
abstract::The contribution of Gal alpha 1,3Gal (alpha-Gal) to cell-mediated organ xenograft rejection is controversial. We have used recombinant lentiviruses encoding a porcine alpha 1,3 galactosyltransferase (alpha 1,3GalT) to obtain alpha-Gal-expressing primary human aortic endothelial cells (HAEC) at a frequency of 70-90%. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324683
更新日期:2004-04-01 00:00:00
abstract::Current concepts of activation of the alternative pathway of complement (APC) focus on the central role of an amplification mechanism triggered by C3b which is covalently bound to the surfact of activating substances. Using sulfated polyanions as model substances, an efficient fluid-phase activation of complement is d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110405
更新日期:1981-04-01 00:00:00
abstract::The interaction of CD40 ligand (CD40L) on activated T cells with CD40 on B cells, monocytes and dendritic cells is essential for humoral immunity and for up-regulation of antigen-presenting cell (APC) functions, as a result of signaling through CD40. There are also some indications that after interaction with CD40, CD...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260732
更新日期:1996-07-01 00:00:00
abstract::Toll-like receptors (TLR) 7 and 8 are closely related members of the TLR family of pathogen-associated molecular pattern recognition receptors and have an important function in activation of innate immune responses upon viral infection. TLR7 can be activated selectively by the guanosine analogue loxoribine, whereas th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535806
更新日期:2006-07-01 00:00:00
abstract::CD40 ligand (CD40L) and IL-4 are sufficient to induce resting murine B cells to divide and switch isotypes from IgM and IgD to IgG1 and IgE. Tracking of cell division following (5- and 6) carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling revealed that B cells expressed IgG1 after three cell divisions, an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<1040::AID-IMM
更新日期:1998-03-01 00:00:00
abstract::Activation of invariant natural killer T (iNKT) cells by treatment with their α-galactosyl ceramide ligand provides therapeutic benefits in several immune inflammatory settings. Given the artificial nature of this stimulation, the natural regulatory functions of iNKT remain uncertain. Addressing this issue in a mouse ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040647
更新日期:2011-02-01 00:00:00
abstract::Endogenous superantigens (SAg) presented by MHC class II IA molecules induce slow-evolving negative selection of alpha beta T cells. The role of both B and gamma delta T cells on the regulation of these SAg-specific alpha beta T cell responses was addressed in IA(b+)IE(b-) C57BL/6 mice bearing genetically induced B ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200109)31:9<2811::aid-immu2811>3
更新日期:2001-09-01 00:00:00
abstract::Previous studies have demonstrated that immunization of BALB/c mice with alpha(1----3) dextran (Dex) is accompanied by a reduction in the subsequent immune response to the acetylcholine receptor (AChR), depending on the timing of the Dex administration relative to AChR challenge. Here, we report that suppression of th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200803
更新日期:1990-08-01 00:00:00
abstract::Based upon the success of using polyclonal, Epstein-Barr virus (EBV)-specific CTL lines for the prophylaxis and treatment of patients with post-transplant lymphoproliferative disease (PTLPD), there is now considerable incentive to develop CTL directed against the sub-dominant EBV antigens EBNA1, LMP1 and LMP2, which a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200103)31:3<947::aid-immu947>3.0
更新日期:2001-03-01 00:00:00
abstract::The diversity of the human immune repertoire and how it relates to a functional immune response has not yet been studied in detail in humanized NOD.SCID.gammac(-/-) immunodeficient mice. Here, we used a multiplex PCR on genomic DNA to quantify the combinatorial diversity of all possible V-J rearrangements at the TCR-b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939480
更新日期:2009-08-01 00:00:00