Abstract:
:Dendritic cells isolated from sheep afferent lymph were examined for their ability to bind soluble protein and peptide antigens labeled with fluorescein both in in vitro assays and following intradermal injection of antigen in vivo. Analysis of dendritic cells by flow cytometry revealed weak direct binding of proteins and peptide antigens. However, the degree of uptake was greatly enhanced in the presence of specific antibody in vitro or if antigen was injected intradermally into antigen-primed sheep. About 60% of dendritic cells possessed the ability to take up antigen in both the in vitro and in vivo experiments. The uptake of antigen occurred very rapidly, reaching maximum values in terms of cell numbers and fluorescence intensity in less than 5 min in vitro and 20-40 min following in vivo challenge. Both sheep IgG subclasses could mediate this effect, but F(ab')2 fragments were ineffective. Procedures adopted to remove complement components from the in vitro test mixtures did not result in any reduction in the binding of antigen by dendritic cells. Two-color flow cytometry analysis of the dendritic cell population further showed that 43% of cells taking up the antigen/antibody complexes were CD1+, suggesting a relationship between these cells and Langerhans' cells or other dendritic cells in skin. The results, thus, indicate that approximately two thirds of sheep afferent lymph dendritic cells bind antigen/antibody complexes via an Fc receptor, a mechanism which could be important in the accentuated accessory function of these cells known to occur following secondary antigen challenge.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Harkiss GD,Hopkins J,McConnell Idoi
10.1002/eji.1830201102subject
Has Abstractpub_date
1990-11-01 00:00:00pages
2367-73issue
11eissn
0014-2980issn
1521-4141journal_volume
20pub_type
杂志文章abstract::Tumor-associated macrophages (TAMs) are key components of the tumor macroenvironment. Cancer- and host cell-derived signals generally drive the functions of TAMs towards an M2-like polarized, tumor-propelling mode; however, when appropriately re-educated. TAMs also have the potential to elicit tumor destructive reacti...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
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更新日期:2011-09-01 00:00:00
abstract::The induction of B cell tolerance to 2,4-dinitrophenyl conjugates of polysaccharide antigens (levan or dextran) was studied in mice primed with keyhole limpet hemocyanin (KLH) or 2,4,6-trinitrophenylated KLH. The relationship of the epitope density of the tolerogen with avidity of B cell receptors (as judged indirectl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060910
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abstract::Pre-B cells which show a delayed development of responsiveness to the B cell mitogen lipopolysaccharide (LPS) are found in murine embryonic blood between day 10 and 14 of gestation. The highest frequency of pre-B cells in embryonic blood is found at day 12 of gestation. All pre-B cells of embryonic blood, isolated at ...
journal_title:European journal of immunology
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更新日期:1980-10-01 00:00:00
abstract::A T3 complex-bearing subpopulation was characterized within an in vivo cycling T4-8- early thymocyte compartment which contains cells constitutively expressing interleukin 2 and transferrin receptors. We show differentiation in vitro of both mature subsets of thymocytes (T3+4+8- and T3+4-8+) from the above T4-8- compa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160611
更新日期:1986-06-01 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma (PPARgamma ), a member of the nuclear receptor superfamily, has recently been described as a modulator of macrophage functions and as an inhibitor of T cell proliferation. Here, we investigated the role of PPARgamma in dendritic cells (DC), the most potent antigen-prese...
journal_title:European journal of immunology
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更新日期:2001-10-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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doi:10.1002/eji.1830140906
更新日期:1984-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040647
更新日期:2011-02-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1984-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2001-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130210
更新日期:1983-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.1830210416
更新日期:1991-04-01 00:00:00
abstract::Heterologous adenovirus-based vectors hold promise as preventative HIV vaccines but their capacity to induce effective T-cell immunity in established infection has not been explored. We vaccinated rhesus macaques chronically infected with SIVmac251 and undergoing antiretroviral therapy (ART) with human adenovirus sero...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839130
更新日期:2009-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<855::AID-IMMU
更新日期:1998-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1999-03-01 00:00:00
abstract::Interleukin (IL)-6 transsignaling plays a pivotal role in the shift from neutrophil to monocyte recruitment at the inflammatory site. Release of neutrophil IL-6 receptor-alpha (IL-6Ralpha, CD126) in its soluble form is a key step of IL-6 transsignaling, however, its physiological inducers are poorly known. Here, we ob...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1993-07-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:2007-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160518
更新日期:1986-05-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1985-05-01 00:00:00
abstract::Antigen-specific CD4(+) T cells are central to natural and vaccine-induced immunity. An ongoing antigen-specific T-cell response can, however, influence surrounding T cells with unrelated antigen specificities. We previously observed this bystander effect in healthy human subjects following recall vaccination with tet...
journal_title:European journal of immunology
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更新日期:2010-04-01 00:00:00