Abstract:
:Mouse peritoneal macrophages activated with interferon-gamma (IFN-gamma) and lipopolysaccharide produce substantial amounts of nitric oxide (NO), which correlates with the elimination of the intracellular protozoan parasite Leishmania major. Both the production of NO and the leishmanicidal function of the activated macrophages can be significantly inhibited by catalase in a dose- and time-dependent manner. These results could not be interpreted by the reduction of H2O2 by catalase since the removal of H2O2 by the addition of glutathione peroxidase had no effect on the NO synthesis or the leishmanicidal function of activated macrophages. Furthermore, catalase did not affect the induction of NO synthase in IFN-gamma-activated macrophages. In contrast, the inhibition of NO synthesis and leishmanicidal activity by catalase was reversed in a dose-dependent manner by the addition of tetrahydrobiopterin, a cofactor of NO synthase. Taken together, these results not only further support the central role of NO as the cytotoxic moiety, but also suggest that hydrogen peroxide may interfere with NO production by affecting the levels of cofactor needed for its synthesis.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Li Y,Severn A,Rogers MV,Palmer RM,Moncada S,Liew FYdoi
10.1002/eji.1830220223keywords:
subject
Has Abstractpub_date
1992-02-01 00:00:00pages
441-6issue
2eissn
0014-2980issn
1521-4141journal_volume
22pub_type
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1993-03-01 00:00:00
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pub_type: 评论,杂志文章
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更新日期:2005-02-01 00:00:00