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A quantitative assay of peptide-dependent class I assembly.

Abstract：

:We have developed a quantitative assay for the measurement of class I assembly induced by peptide. We have applied this assay to H-2Db, Kb and HLA-A2.1 with a panel of 49 overlapping peptides derived from HIV-1 gag protein. We find that the effects of peptide on assembly form a continuous distribution. By defining positives as those that increase the concentration of folded heavy chains more than three standard deviations from the control we show that 7/48 bind A2.1, 11/49 bind Db and 7/47 bind Kb. The assembly assay contrasts with solid-phase assays in being more discriminating (fewer peptides binding any given class I molecule), and showing less overlap in the patterns of peptides bound by the three class I molecules.

journal_name

Eur J Immunol

journal_title

European journal of immunology

authors

Elvin J,Cerundolo V,Elliott T,Townsend A

doi

10.1002/eji.1830210909

subject

Has Abstract

pub_date

1991-09-01 00:00:00

pages

2025-31

issue

9

eissn

0014-2980

issn

1521-4141

journal_volume

21

pub_type

杂志文章

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