Abstract:
:The mechanism of synergistic complement-mediated lysis of rat red cells was investigated using rat monoclonal antibodies against class I RT1Aa antigens. The increased lytic activity when using two antibodies simultaneously is due to the increase in the number of activated C1 molecules on the cell surface and this results from (a) an increase in the number of binding sites for C1q, (b) an increase in the functional affinity constant for C1q binding and (c) an increase in the rate of activation of C1. Complete lysis of red cells was only achieved if one member of the synergistic pair was of the gamma 2b isotype, and this isotype was the only one to which binding of 125I-labeled C1q could be detected. A partial synergistic effect was seen using an F(ab')2 fragment of antibody. Increased uptake and activation of C1 probably results both from the presence of two antibodies attached to each antigen molecule and from the formation of antigen-antibody catenars.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Hughes-Jones NC,Gorick BD,Howard JCdoi
10.1002/eji.1830130806subject
Has Abstractpub_date
1983-08-01 00:00:00pages
635-41issue
8eissn
0014-2980issn
1521-4141journal_volume
13pub_type
杂志文章abstract::NK cells and cytotoxic T lymphocytes can induce apoptosis in virus-infected and transformed target cells via the granule exocytosis pathway. The key components of the cytolytic granules are perforin and several serine esterases, termed granzymes. While the cellular distribution of human granzymes A (GrA) and B (GrB) h...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526122
更新日期:2005-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200212)32:12<3395::AID-IMMU3395>
更新日期:2002-12-01 00:00:00
abstract::Defined tumor-associated antigens (TAA) are attractive targets for anti-tumor immunotherapy. Here, we describe a novel genome-wide approach to identify multiple TAA from any given tumor. A panel of transplantable thymomas was established from an inbred p53-/- mouse strain. The resulting tumors were examined for gene e...
journal_title:European journal of immunology
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更新日期:2001-04-01 00:00:00
abstract::Humanized BALB/c mice (termed trimera mice) conditioned by lethal total body irradiation and bone marrow transplantation from SCID mice have been described to support rapid engraftment of human peripheral blood mononuclear cells (PBMC) and the induction of strong B and T cell responses after immunization in vivo. More...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<2071::aid-immu2071>3
更新日期:2001-07-01 00:00:00
abstract::The aim of this study was to investigate the effects of IL-17-producing T cells, including Th17 and Tc17 cells, on acute graft-versus-host disease (aGVHD) in patients who had undergone granulocyte colony-stimulating factor (G-CSF)-mobilised peripheral blood progenitor cell (PBPC) and G-CSF-primed bone marrow (G-BM) tr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040793
更新日期:2011-02-01 00:00:00
abstract::Stimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists, or antagonists. Fo...
journal_title:European journal of immunology
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doi:10.1002/eji.1830271241
更新日期:1997-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1997-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100709
更新日期:1980-07-01 00:00:00
abstract::Mice with homologous disruption of the gene coding for either the p35 subunit or the p40 subunit of interleukin-12 (IL-12) and derived from a strain genetically resistant to infection with Leishmania major have been used to study further the role of this cytokine in resistance to infection and the differentiation of f...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260722
更新日期:1996-07-01 00:00:00
abstract::Mice deficient for the adapter protein SLP65 (BLNK) show a partial block in early B cell development, reduced numbers of mature B cells in the periphery, an absence of B1 cells and a reduction of IgM and IgG3 serum immunoglobulin levels. A strikingly similar phenotype is observed in Btk-deficient mice. To investigate ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<2164::aid-immu2164>3
更新日期:2001-07-01 00:00:00
abstract::Type I IFN are cytokines which play a central role in host resistance to viral or microbial infections and are important components linking innate and adaptive immunity. We and others have previously demonstrated that the production of IFN-beta by DC following bacterial infections or TLR triggering influences, in an a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737566
更新日期:2007-12-01 00:00:00
abstract::IL-23, a proximal regulator of IL-17, may be a major driving force in the induction of autoimmune inflammation. We have used a model of subdiabetogenic treatment with multiple low doses of streptozotocin (MLD-STZ; 4 x 40 mg/kg body weight) in male C57BL/6 mice to study the effect of IL-23 on immune-mediated beta cell ...
journal_title:European journal of immunology
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更新日期:2006-01-01 00:00:00
abstract::Alum has been the only adjuvant licensed for human vaccines for decades and is still widely used, but its mechanism of action remains obscure. Recently, the NLRP3 inflammasome has been linked to the immunostimulatory properties of alum and other particulate adjuvants, although it is disputed to what degree NLRP3 is ge...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
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更新日期:2010-03-01 00:00:00
abstract::This Viewpoint series provides authoritative and detailed outlines of exciting areas of DC research. Some of the subjects that frequently come up include development of DC; distribution of DC in lymphoid and non-lymphoid tissues such as skin, intestine and lung; different forms or subsets of DC; and the role of DC in ...
journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.201040733
更新日期:2010-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160702
更新日期:1986-07-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1989-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2003-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271024
更新日期:1997-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
abstract::Fourteen noncytotoxic human helper T cell clones were examined for autocrine proliferative responses and cytotoxicity to tumor cells after stimulation with 12-O-tetradecanoylphorbol 13-acetate (TPA) and ionomycin (Io). Although all clones responded to alloantigen, they could be divided into two groups based on their p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180729
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abstract::Development of Th1 and Th2 effector lymphocytes is driven primarily by IL-12 or IL-4, but is also influenced by the strength of antigenic stimulation. However, the mechanism by which TCR signaling contributes to T cell polarization remains elusive. We show that in the presence of IL-12 a short TCR stimulation can lead...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<4092::AID-IMM
更新日期:1999-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344072
更新日期:2014-07-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章,评审
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更新日期:2012-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131106
更新日期:1983-11-01 00:00:00
abstract::The mechanisms responsible for major histocompatibility complex (MHC)-linked unresponsiveness are still poorly understood. Here we examine the cellular events that follow when B10. A mice are immunized with cow insulin, an antigen to which they make no apparent immunologic response. Despite the fact that there is no d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180122
更新日期:1988-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445422
更新日期:2016-01-01 00:00:00