Abstract:
:B cells isolated from germinal centers (GC) of immune mice 2-5 days after antigen (Ag) challenge migrate in response to chemotactic signals, whereas GC B cells isolated at other times and resting B cells do not. Since B cells are in direct contact with follicular dendritic cells (FDC) in GC we reasoned that FDC might play a role in enabling B cells to become chemotactically active. Resting B cells were co-cultured with FDC either with or without anti-mu-dextran (anti-mu-dex) as an Ag surrogate and/or recombinant interleukin (rIL)-4 as a T cell surrogate. After 3 days, the B cells were isolated and their migration to chemotactic factors contained in zymosan-activated serum assessed in microchemotaxis chambers. B cells incubated alone or with anti-mu-dex or rIL-4 showed minimal migration, which could be increased if both anti-mu-dex or rIL-4 were present. However, maximal migration was obtained when B cells were cultured with FDC, and this was not increased by addition of anti-mu-dex and/or rIL-4, indicating that the FDC signal was a primary signal and did not require pre-activation of the B cells. Checkerboard analysis using variation in concentration and location of the chemoattractant in chemotaxis chambers indicated that both chemotaxis and chemokinesis occurred. B cell migration began within 6 h of culture, peaked by 48 h and decreased thereafter. Removal of FDC or interference with FDC-B cell contact ablated or significantly decreased induction of B cell migration. Furthermore, induction did not require functional T cells. These data indicate that FDC can induce resting B cells to become responsive to chemotactic signals.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Burton GF,Kupp LI,McNalley EC,Tew JGdoi
10.1002/eji.1830250437subject
Has Abstractpub_date
1995-04-01 00:00:00pages
1105-8issue
4eissn
0014-2980issn
1521-4141journal_volume
25pub_type
杂志文章abstract::CD4+ alpha beta T cell populations that develop in mice deficient in MHC class II (through 'knockout' of either the Aalpha, or the Abeta chain of the I-A(b) molecule) comprise a major 'single-positive' (SP) CD4+ CD8- subset (60-90%) and a minor 'double-positive' (DP) CD4+ CD8alpha beta+ subset (10-40%). Many DP T cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324463
更新日期:2004-03-01 00:00:00
abstract::To investigate the role of TCR-pMHC interaction in regulating lung CD8 tissue-resident T cell (TR ) differentiation, polyclonal responses were compared against NP366-374 /Db and PA224-233 /Db , two immunodominant epitopes that arise during influenza A infection in mice. Memory niches distinct from iBALTs develop withi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747174
更新日期:2018-01-01 00:00:00
abstract::Interferon (IFN) was detected upon co-culture of cloned Sendai virus (SV)-specific T lymphocyte with SV-presenting syngeneic stimulator cells. The antiviral activity was defined as IFN-alpha, beta. The T cell clones, upon contact with antigen-presenting stimulator cells, stimulated adherent cells present in the stimul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121212
更新日期:1982-12-01 00:00:00
abstract::Immunomodulatory treatment paradigms have been applied to animal models of T cell-mediated autoimmune diseases in an attempt to develop an immunospecific and non-toxic form of therapy which can be applied to humans. These treatment paradigms are often directed to T cells with a restricted T cell receptor repertoire or...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240926
更新日期:1994-09-01 00:00:00
abstract::A monoclonal antibody (12-15) reactive with the mouse CD2 was used to study the expression of the antigen in different lymphoid cell subsets. By two-color immunofluorescence using B or T cell-specific reagents and cell sorting in combination with biochemical analysis we provide evidence that the CD2 antigen is present...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190826
更新日期:1989-08-01 00:00:00
abstract::LL-37, derived from human cathelicidin, stimulates immune responses in neutrophils. Although FPR2 and P2X7 were proposed as LL-37 receptors, we have shown that among 21 neutrophil receptors only CXCR2 was down-regulated by LL-37. LL-37 functions similarly to CXCR2-specific chemokines CXCL1 and CXCL7 in terms of recept...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939496
更新日期:2009-11-01 00:00:00
abstract::Genetic control has been studied of the response to the encephalitogenic nonapeptide (NP) determinant of myelin basic protein (BP) in inbred guinea pigs of strains resistant or susceptible to induction of experimental autoimmune encephalomyelitis (EAE). By studying bone marrow-reconstituted animals, we found that susc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110409
更新日期:1981-04-01 00:00:00
abstract::Previous studies from our laboratory have shown that prolonged exposure of mouse macrophages to IFN-beta interferes with their subsequent ability to become activated for tumor cell killing. Data reported here show that such inhibition is due to reduced production of NO, resulting from decreased transcription of the ge...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200006)30:6<1551::AID-IMMU1551>3
更新日期:2000-06-01 00:00:00
abstract::The effect of fractionated total lymphoid irradiation (TLI) on the maturation of B cells was examined in mice. At various times after irradiation of the mice, their spleen cells were tested for the mitogenic responses to dextran sulfate and lipopolysaccharide. In parallel the cells were stained with fluorescent anti-T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130109
更新日期:1983-01-01 00:00:00
abstract::The pool of hematopoietic stem cells (HSCs) in the bone marrow is a mixture of resting, proliferating, and differentiating cells. Long-term repopulating HSCs (LT-HSC) are routinely enriched as Lin- Sca1+ c-Kit+ CD34- Flt3- CD150+ CD48- cells. The Flt3 ligand (Flt3L) and its receptor Flt3 are important regulators of HS...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646730
更新日期:2017-09-01 00:00:00
abstract::CBA/J mice immunized with thyroglobulin (Tg) develop an experimental autoimmune thyroiditis (EAT) with lymphocytic infiltration of the thyroid glands, autoantibodies to Tg and occurrence of EAT-specific T cells. When these mice were treated for 4 weeks after immunization with 1 mg/week of a monoclonal antibody (mAb) t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230143
更新日期:1993-01-01 00:00:00
abstract::Immunotherapy generally fails to induce tumour regression in spontaneously arising tumours. Failure is attributed to both tumour-related factors and an ineffective immune response. As a model of tumour immunotherapy, without the confounding effects of potential tumour-determined mechanisms of immune evasion, we studie...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737599
更新日期:2008-01-01 00:00:00
abstract::In this report, we demonstrate that gp40, a molecule previously shown to be expressed by thymic epithelial cell lines in vitro and by thymic epithelial cells in vivo, is the murine homolog of human Ep-CAM, a calcium-independent homotypic adhesion molecule. gp40 is also expressed at low levels by thymocytes and periphe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260220
更新日期:1996-02-01 00:00:00
abstract::The CD38 antigen displays restricted functional associations with surface molecules involved in immune system and complement. Capping of the CD38 molecule in normal or neoplastic T cells is followed by rapid and specific co-modulation of the CD3-T cell receptor (TcR) complex. In normal and tumor cells of B lineage, CD...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231005
更新日期:1993-10-01 00:00:00
abstract::The importance of human herpesvirus 6 (HHV-6) species as human pathogens is increasingly appreciated. However, we do not understand how infection is controlled in healthy virus carriers, and why control fails in patients with disease. Other persistent viruses are under continuous surveillance by antigen-specific T cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242439
更新日期:2012-11-01 00:00:00
abstract::This report describes the study of the expression of an idiotype in the human population which is associated with antibodies to N-acetyl-D-glucosamine (GlcNAc) present in most human sera presumably due to streptococcal infections. The idiotype is identified with antisera and monoclonal antibodies prepared against the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130402
更新日期:1983-04-01 00:00:00
abstract::Membrane-bound IgG was found only on old populations of platelets from normal individuals. This IgG could be dissociated from senescent cells by repeatedly heating the cells. Heat-eluted IgG (He-IgG) prepared from senescent red blood cells was capable of binding to either heat-treated old platelets or Vibrio cholerae ...
journal_title:European journal of immunology
pub_type: 杂志文章,收录出版
doi:10.1002/eji.1830131207
更新日期:1983-12-01 00:00:00
abstract::The functional role of the T8 antigen of human T cells was studied by inhibition with anti-T8 monoclonal antibodies (mAb) of the cytotoxic action of T8+ cytotoxic T lymphocyte clones (CTL). All clones were allospecific and directed against HLA-B7. The ability of seven different anti-T8 mAb to inhibit the cytotoxicity ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161109
更新日期:1986-11-01 00:00:00
abstract::Aging is accompanied by altered immunity, resulting in a variable state of poorly understood immunodeficiency. While both the numbers and the functionality of naïve T cells are decreased by aging, the impact of these changes upon immune defense against bacterial pathogens in vivo remains understudied. Using a model of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041141
更新日期:2011-05-01 00:00:00
abstract::We tested the effect of interferon (IFN)-alpha on the specific anti-2,4,6-trinitrophenyl (TNP) response induced by TNP-conjugated polyacrylamide beads in cultures of purified human B cells. IFN-alpha alone had no effect. Interleukin 2 (IL2) alone had a restorative effect which could be considerably (10X) enhanced by I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180912
更新日期:1988-09-01 00:00:00
abstract::Bispecific antibodies binding to the TCR/CD3 complex and to a tumor-associated surface molecule can be used to target cytotoxic T lymphocytes against tumor cells. We reasoned that high-affinity binding to CD3 may reduce the efficiency of T cell stimulation and target the bispecific reagent to T cells rather than to tu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200211)32:11<3102::AID-IMMU3102>
更新日期:2002-11-01 00:00:00
abstract::DT9301-0229737 the TcR are believed to provide the peptide fragments bound to major histocompatibility (MHC) molecules. TcR have an immunoglobulin (Ig)-like structure and, in an analogous manner to antigen recognition by Ig, the third complementarity determining regions (CDR3) of the TcR are believed to provide the pr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240519
更新日期:1994-05-01 00:00:00
abstract::When cytotoxic titrations were made of rabbit anti-mouse brain and anti-Thy-1.2 sera against mouse lymphoid cells in the presence of sodium barbital or xylocaine, it was observed that a proportion of T cells were much more sensitive (50 x) than the remainder. This effect could be detected with ethidium or fluorescein ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050408
更新日期:1975-04-01 00:00:00
abstract::X-linked agammaglobulinemia (XLA) is a humoral immunodeficiency disease in man, characterized by an arrest in B lymphocyte differentiation at the precursor B cell stage. The structure of expressed immunoglobulin (Ig) kappa light (L) chain rearrangements of nine B lymphoblastoid cell lines from one XLA patient was inve...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230306
更新日期:1993-03-01 00:00:00
abstract::Muramyl peptides are the building blocks of bacterial peptidoglycan, and their biological functions in mammals have been extensively studied. In particular, muramyl peptides trigger inflammation, contribute to host defense against microbial infections, and modulate the adaptive immune response to antigens. These bacte...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040827
更新日期:2011-05-01 00:00:00
abstract::B cells have multiple functions in adaptive immunity, including antibody production, antigen presentation and regulation of T-cell responses. Recent evidences indicate that B cells have more subsets than previously thought and may have non-classical functions, such as involvement in innate immunity and immune regulati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040840
更新日期:2011-03-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) and natural killer (NK) cells elaborate a cytolytic protein named perforin or cytolysin. It was widely held that, in vivo, high quantities of perforin are not present in resting lymphocytes and are usually produced only by activated lymphocytes found under pathological conditions. Until n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230907
更新日期:1993-09-01 00:00:00
abstract::The CD11c(+) cell population in the non-parenchymal cell population of the mouse liver contains dendritic cells (DC), NK cells, B cells and T cells. In the hepatic CD11c(+) DC population from immunocompetent or immunodeficient [recombinase-activating gene-1 (RAG1)(-/-)] C57BL/6 mice (rigorously depleted of T cells, B ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324336
更新日期:2004-02-01 00:00:00
abstract::The recently discovered B6.C-H-2bm12 (bm12) histocompatibility mutant bears a mutation of the I-A subregion of the H-2b haplotype. We have studied cytotoxic T lymphocytes (CTL) generated in secondary mixed lymphocyte culture (MLC) between the bm12 mutant and the strain of origin C57BL/6 (B6). In both directions, stron...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110317
更新日期:1981-03-01 00:00:00
abstract::In vitro prepared antigen-specific helper factors reactive to the synthetic polypeptide antigens poly-L(Tyr, Glu)-poly-DLAla--poly-LLys [(T, G)-A--L] or LGlu60-LAla30-LTyr10 (GAT) and bearing Ia determinants were analyzed serologically to determine the nature of the Ia determinants they expressed. I subregion-specific...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090703
更新日期:1979-07-01 00:00:00